PROCESS BIOCHEMISTRY
·
2020
·
IF 2.952
Jian-Ying Dai,Wen-Tian Guan,Zhi-Long Xiu
Inulin could be converted to bio-based chemicals by an inulinase producer without external inulinase, and the production of 2,3-butanediol was less than 50 g/L. In this work, a novel inulinase producer of Klebsiella pneumoniae H3 was isolated, and inulinase catalytic properties as well as 2,3-butanediol fermentation were investigated. The enzyme was an intracellular inulinase with an optimal pH of 6 ∼ 7 and a temperature of 30 °C. The use of inulin by H3 was dependent on the degree of polymerization (DP), and the average DP of inulin in fermentation broth increased from 2.82 to 8.08 in 24-h culture of batch fermentation. Acidic pretreatment was developed to increase inulin utilization by adjusting medium pH to 3.0 prior to sterilization. In batch fermentation with optimized medium and fermentation conditions, the concentration of target product (2,3-butanediol and acetoin) was 80.4 g/L with a productivity of 2.23 g/(L⋅h), and a yield of 0.426 g/g inulin.
研究领域:
微生物学
·
生物技术
·
酶学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2016
·Vol. 11
·期 2
·696-703
·
IF 0.521
Kong Lu,Zhao Xin-Huai
Three acids (acetic, propionic and butyric acids) yielded from simulated colonic fermentation of inulin and xylo-oligosaccharides (XOS) in the absence and presence of six exogenous strains were investigated in this study. Both inulin and XOS were fermented by fecal extract (FE) prepared from healthy adults and by an exogenous strain for 48 h, and three acids generated in fermentation products at five time periods were detected by gas chromatography. Inulin and XOS during fermentation were capable of producing more propionic and butyric acids, respectively, whilst inoculation using one strain culture into fermentation systems mostly increased yields of three acids. In inulin-containing fermentation systems, these strains increased yields of propionic and butyric acids by 6–34 and 206–250%, respectively. In XOS-containing fermentation systems, these strains did not enhance yield of propionic acid, but increased yield of butyric acid by 50–96%. In general, Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus rhamnosus, Enterococcus faecalis and Enterococcus faecium were more efficient than Lactobacillus acidophilus to increase yields of propionic and butyric acids. It is thus concluded that the six strains in total displayed beneficial effects on simulated fermentation of inulin and XOS through enhancing yields of three acids especially propionic and butyric acids with healthcare functions.
研究领域:
微生物学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 10
·1467
·
IF 5.561
Minxing Xue,Jin Wang,Meigui Huang
Cinnamaldehyde as an antioxidant was encapsulated in inulin-modified nanoliposomes in order to improve its physical and antioxidant stability. The microstructure, particle size and volume distribution of cinnamaldehyde liposomes were characterized by atomic force microscopy (AFM) and dynamic light scattering (DLS). The particle size and polydispersion index (PDI) values of the inulin modified liposomes were 72.52 ± 0.71 nm and 0.223 ± 0.031, respectively. The results showed that the liposomes after surface modification with inulin remained spherical. Raman and Fourier transform infrared (FTIR) spectra analysis showed that hydrogen bonds were formed between the inulin and the liposome membrane. Inulin binding also restricted the freedom of movement of lipid molecules and enhanced the order of the hydrophobic core of the membrane and the polar headgroup region in lipid molecules. Therefore, the addition of different concentrations of inulin influenced the permeability of the liposome bilayer membrane. However, when inulin was excessive, the capacity of the bilayer membrane to load the cinnamaldehyde was reduced, and the stability of the system was reduced. Additionally, the encapsulation efficiency (EE) and retention rate (RR) of cinnamaldehyde from inulin-modified liposomes during storage were determined. The EE value of the inulin modified liposomes was 70.71 ± 0.53%. The liposomes with 1.5% inulin concentration had the highest retention rate (RR) and the smallest particle size during storage at 4 °C. The addition of inulin also enhanced the thermal stability of the liposomes. Based on the results, the surface modification improved the oxidation stability of liposomes, especially the DPPH scavenging ability. In conclusion, these results might help to develop inulin as a potential candidate for the effective modification of the surface of liposomes and provide data and conclusions for it.Keywords:liposomes;surface modification;inulin;physical stability
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Hazardous Materials Advances
·
2025
·
IF 7.7
Peigen Chen,Lina Chen,Xing Yang,Tingting Li,Cong Fang
The pervasive contamination by Di(2-ethylhexyl) phthalate (DEHP) and nanoplastics (NPs) poses a significant threat to reproductive health, yet the combined effects of these pollutants on endometrial receptivity remain poorly understood. Our study investigates the impact of chronic DEHP and NPs co-exposure on endometrial ciliary function in mice and explores the potential protective role of dietary inulin. Utilizing a multifaceted approach—including transcriptomic analysis, immunohistochemistry, scanning electron microscopy, and immunofluorescence—we reveal that DEHP and NPs synergistically impair endometrial cilia, crucial for embryo implantation. Remarkably, inulin supplementation restores ciliary structure and function, mitigating the detrimental effects of these pollutants. Our findings suggest that dietary interventions could offer a practical strategy to counteract the reproductive toxicity of environmental contaminants, providing new insights into maintaining reproductive health in polluted environments. This research underscores the urgency of addressing environmental pollutants and highlights the potential of inulin as a protective agent against reproductive toxicity.
研究领域:
生殖生物学
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环境医学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Xiliang Yu,Chen Li,Mengdi Zhou,Sangeeta Prakash,Xiuping Dong
Many countries have aging populations, which continue to grow. Compound diets based on the nutritional needs of older adults were prepared using mixed grain rice and cooked surimi. The textural, rheological, and 3D-printing properties were regulated through the synergy between carrageenan (CG) and inulin (IN) (The total addition of CG and IN is 1.5% (w/w) and the ratio between CG and IN was 3/1, 1/1, 1/3(w/w)). The results show that all compound diets have shear thinning behavior, and the apparent viscosity, support properties, and recovery rate increased with increased CG/IN. The compound diets with a 3/1 CG/IN ratio had the best shear recovery rate (80.95%). Compared with the control group, adding CG/IN increased the yield stress and extrusion force of the compound diets, which decreased with the increased IN ratio. The combination of CG and IN in compound diets could facilitate easier chewing and safer swallowing. When the CG/IN ratio was 3/1or 1/1, the compound diets had the best 3D-printing properties, those which have the highest degree of similarity to the example design graphic and the most pronounced line texture. When the CG and IN were added, the hardness, springiness, and chewiness of the compound diets were enhanced, and the microstructure became denser and more regular. The compound diets are suitable for people with dysphagia and can be classified into levels 5–6 in the International Dysphagia Diet Standardization Initiative framework.
研究领域:
食品科学
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营养学
·
老年医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2017
·
IF 0
Peng Lu,Zhi-Ping Zhao,Xing-Ya Wang,Gong-Jia Lan,Xiao-Lan Wang
Developing a green hydrolysis process of biomass for the production of the value-added compounds has been widely concerned. This article aimed at elucidating the structure-property relationship of ionic liquids (ILs) as catalysts, i.e., effect of the chemical structures of anion and cation of ILs on their acidity and catalytic properties of inulin hydrolysis. Twenty kinds of imidazole-based ILs and one kind of quaternary ammonium ILs were synthesized. Inulin hydrolysis for the reducing sugar production was investigated with a series of functionalized ILs under moderate temperature (75 °C) and atmospheric pressure. The chemical structure, acidity and catalytic property of ILs were experimentally characterized and theoretically analyzed. The possible catalytic hydrolysis mechanisms of polysaccharide or lignocellulose by ILs were detailedly analyzed. This work demonstrated that the catalytic performance of catalysts depends not only on its acidity, but also on the chemical structure. It was revealed that imidazole-based ILs showed better catalytic performance than quaternary ammonium ILs in the inulin hydrolysis system, and SO 3 H-functionalized sulfonate ILs obtained more efficient catalytic property because the hydrolysis reaction was catalyzed by the synergy of anion and cation, compared with the sulfuric acid with stronger acidity, which can cause serious equipment corrosion in biomass hydrolysis process. In all these tested ILs, (1-(4-sulfonic acid)-butyl-3-ethylimidazolium hydrogen sulfate) ([C 2 MIM-PS][HSO 4 − ]) exhibited the best catalytic performance, and the yield of reducing sugar was 100%. Understanding the structure-property relationship of ILs will be helpful to design the covalent immobilization strategy of ILs to improve its reusability and eliminate the potential harmfulness to the environment.
研究领域:
绿色化学
·
催化科学
✓ 已验证
产品关联
DOI
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shiwen Zhao,Danyang Wang,Qiong Zhou,Beibei Wang,Zhao Tong,Honglei Tian,Jianke Li,Yuhuan Zhang
Nanozymes with oxidase or peroxidase-mimicking activity have emerged as a promising alternative for disinfecting resistant pathogens. However, further research and clinical applications of nanozymes are hampered by their low in vivo biosafety and biocompatibility. In this study, inulin-confined gold nanoparticles (IN@AuNP) are synthesized as an antibacterial agent via a straightforward in situ reduction of Au 3+ ions by the hydroxyl groups in inulin. The IN@AuNP exhibits both peroxidase-mimicking and oxidase-mimicking catalytic activities, of which the maximum reaction velocity ( V max ) for H 2 O 2 is 2.66 times higher than that of horseradish peroxidase. IN@AuNP can catalyze the production of reactive oxygen species (ROS), resulting in effective antibacterial behavior against both Gram-negative ( Escherichia coli ) and Gram-positive ( Staphylococcus aureus ) bacteria. Abundant hydroxyl groups retained in inulin endow the nanozyme with high adhesion to bacteria, reducing the distance between the captured bacteria and ROS, achieving an antibacterial ratio of 100 % within 1 h. Importantly, due to the natural biosafety and non-absorption of the dietary fiber inulin, as well as the inability of inulin-trapped AuNP to diffuse, the IN@AuNP exhibits high biosafety and biocompatibility under physiological conditions. This work is expected to open a new avenue for nanozymes with great clinical application value.
研究领域:
生物医学工程
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2020
·
IF 7.183
Han Jiang,Tao Wang,Shuo Li,Zhi-Ping Zhao
Catalytically active membranes are the core components of the membrane reactors . Polysulfone (PSF) grafted with ionic liquids (ILs), PSF-DG-ILs, was prepared via introducing chloromethyl group into benzene ring of PSF, then grafting ILs, imidazolyl acid ILs ([(CH 2 ) 3 SO 3 HVIm]HSO 4 ), by chemical bonding. Then PSF-DG-ILs membranes were prepared by non-solvent induced phase separation technique for hydrolysis of inulin. The effects of coagulation bath, grafting degree, and various dope blending on structure, physico-chemical characters, and catalytic performance of membranes were evaluated. And results revealed that catalytic performance could be efficiently enhanced by choosing ethanol as coagulation compared with ethyl acetate, which was mainly attributed to surface segregation. Membrane mechanical strength decreased with the enhancing ILs grafting degree, while the catalytic performance increased. However, PSF-DG-ILs with high degree of ILs grafting lost its membrane-forming property. The mechanical and catalytic properties of the membrane were synergistically enhanced by blending PSF-DG-ILs polymers with high and low ILs grafting degree, then the resulting membranes broke the trade-off effect between mechanical strength and catalytic ability. Specifically, membrane fabricated by mixing the polymers with ILs grafting degree of 0.31 and 0.59 integrates optimized mechanical property and catalysis performance. The strength and strain of this blending membrane are 3.17 MPa and 14.10%. The average pore size and porosity are 215.5 nm and 46.2%. The yield of inulin hydrolysis can reach 88.22% with 20 wt% concentration of inulin at 75 °C using a flux of 23.4 L/(m 2 ·h). Therefore, the PSF-DG-ILs membrane displayed promising potential for the application in biomass conversion .
研究领域:
生物工程
·
材料科学
✓ 已验证
DOI
高影响因子
Foods
·
2019
·Vol. 8
·期 6
·181
·
IF 3.011
Rui Li,Qi Ding,Xin-Huai Zhao
The impact of milk fortification on the microbiological and physicochemical properties of a set-type skimmed yoghurt using three commercial soluble prebiotics (inulin, iso-malto-oligosaccharides, and xylo-oligosaccharides) at either 3 or 5 g/kg was assessed. The three prebiotics had an insignificant impact on yoghurt fermentation because all yoghurt samples had similar titratable acidity and similar pH values after their lactic acid fermentation. Regarding the control yoghurt samples without prebiotics usage, the prebiotics-fortified yoghurt samples showed no difference in their main chemical compositions, hardness, syneresis extent, and apparent viscosity (p> 0.05), but had a slightly higher lactic acid content and a viable quantity of starter strains. All yoghurt samples had the same acetic acid content, while propionic and butyric acids were not produced. Yoghurt storage at 4 °C for 21 day gave these yoghurt samples decreased pH values and a viable quantity of starter strains (especiallyLactobacillus delbrueckiisubsp.bulgaricus) and unchanged acetic acid; however, it increased lactic acid contents. Overall, prebiotics fortification up to 5 g/kg had a completely insignificant impact on the fermentation and quality attributes of yoghurt samples but could possibly improve the health of consumers due to higher dietary fibers and starter strain populations.Keywords:set-type skimmed yoghurt;inulin;iso-malto-oligosaccharides;xylo-oligosaccharides;yoghurt quality
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Dongdong Wang,Feifei Sun,Chunbo Lu,Peng Chen,Zhaojie Wang,Yuanhao Qiu,Haibo Mu,Zehong Miao,Jinyou Duan
Colorectal cancer is one of the most common types of tumor in the world. Here we developed a lipoic acid esterified polysaccharide (inulin) delivery system for tanshinone IIA to treat colorectal cancer in vitro . The release of tanshinone IIA in the system was highly responsive to glutathione , which is commonly abundant in cancer cells. In addition, this drug delivery system was proliferative to Bifidobacterium longum , the common inhabitant of human intestine. Thus, this strategy might be useful to improve colon cancer therapy efficacy of anticancer drugs and meanwhile promote the growth of beneficial commensal flora in the gut.
研究领域:
药剂学
·
肿瘤学
·
肠道微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Sciences-Basel
·
2018
·Vol. 8
·期 5
·770
·
IF 1.689
Peng Lu,Yong Cao,Xiaolan Wang
Macroporous cardo polyetherketone (PEK-C) powder catalyst bearing covalently ionic liquids (PEK-C-ILs) was prepared, it exhibited a high catalytic performance for biomass hydrolysis. A hydrolysis mechanism of inulin over this new catalyst was proposed as well. The influences of degree of chloromethylation of PEK-C, catalyst dosage, reaction temperature, pore size of PEK-C-ILs and inulin concentration were investigated. A high conversion of inulin of 99.7% was obtained under the optimal reaction conditions. Meanwhile, the hydrolysis kinetics was studied under different conditions. The proposed kinetic model of inulin hydrolysis catalyzed by PEK-C-ILs was established and it successfully predicted the inulin hydrolysis in wider ranges of experimental conditions. The results demonstrated that the catalytic inulin hydrolysis performance of PEK-C-ILs is better than that of homogenous ILs due to that there was a high-density spatial distribution of active sites on the surface and inner pore wall of PEK-C after the ILs was immobilized. Furthermore, the stability including chemical structure and physical appearance and reusability tests showed that PEK-C-ILs catalyst could be effectively separated and recovered from hydrolysates and has excellent reusability. Importantly, using the PEK-C-ILs as catalyst makes the recycling of ILs simplified, efficient and the hydrolysis process more economical. At the same time, it avoided the potential hazards of homogenous ILs to the environment. Therefore, it is a kind of green catalyst with potential application prospect in many catalysis fields.Keywords:cardo polyetherketone;ionic liquids;immobilization;macroporous powder catalyst;inulin hydrolysis
研究领域:
化学工程
·
生物质能
·
催化科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yonggan Sun,Shanshan Zhang,Huijun He,Haihong Chen,Qixing Nie,Song Li,Jiaobo Cheng,Baojie Zhang,Zhitian Zheng,Shijie Pan,Ping Huang,Lu Lian,Jielun Hu,Shaoping Nie
Dietary fiber is crucial for human health mainly due to its impact on gut microbiota structure and metabolites. This study aimed to investigate the impact of Dendrobium officinale polysaccharides (DOP) and two common fibers (β-glucan and inulin) on the gut microbiome structure and metabolic profile in vitro . Fecal samples were obtained from 30 healthy volunteers, which were then individually subjected to fermentation with each type of fiber. The results revealed that all fibers were efficiently degraded by gut microbiota, with DOP exhibiting a slower fermentation rate compared to β-glucan and inulin . The fermentation of all fibers led to a significant increase in the production of short-chain fatty acids (SCFAs) and a reduction in branched-chain fatty acids (BCFAs), sulfides, phenols, and indole. Moreover, the abundance of unclassified Enterobacteriaceae , which was positively correlated with sulfide, phenols, and indole levels, was significantly reduced by all fibers. Additionally, DOP specifically promoted the growth of Parabacteroides , while β-glucan and inulin promoted the growth of Bifidobacterium and Faecalibacterium . Taken together, these findings enhance our understanding of the role of DOP, β-glucan, and inulin in modulating gut microbiota and metabolites, where the fermentation with fecal bacteria from different volunteers could provide valuable insights for personalized therapeutic approaches.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）