Coatings
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2022
·Vol. 12
·期 3
·385
·
IF 3.236
Xiaotong Zhou,Liu Liu,Jianke Li,Lin Wang,Xueying Song
To prolong the storage period of blueberry and improve its shelf-life quality, an edible coating based on chitosan was developed, and different contents of pectin were added to the coating. In this study, Jerusalem artichoke residue was used as a source of pectin, which is a byproduct of the processing of inulin. The extracted pectin has a low cost and high quality, which is very suitable for the preservation of coating. The coating was prepared and the chemical properties of the coating were characterized by SEM, XRD, TG, and FTIR. The barrier properties of the coating were analyzed by thickness, water content, solubility, and water vapor permeability. The results showed that the pectin coating exhibited excellent performance in blueberry preservation. Following 16 days of storage, the decay and weight loss rates of blueberry treated with 0.2% pectin coating decreased by 33 and 22%, respectively. Moreover, the organic acid consumption of the coated blueberry slowed and the anthocyanins were better preserved. As a low-cost, safe, and efficient technology, the pectin chitosan composite coating has significant potential in the berries preservation industry.Keywords:Jerusalem artichoke;pectin;coating;blueberry preservation
研究领域:
食品科学
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植物化学
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DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 8
·1065
·
IF 5.561
Xiaotong Zhou,Liu Liu,Jianke Li,Lin Wang,Xueying Song
The utilization of industrial by-products is becoming more and more important for resource utilization. In this study, soluble dietary fiber (SDF) was extracted from Jerusalem artichoke residue, and a series of characterizations of SDF were carried out. The results showed that SDF had good properties. SDF (0%, 0.1%, 0.2%, 0.3%, and 0.4%) and chitosan (2%) were further used to prepare the coating that was used for the preservation of blueberry. The chemical structure of the film was obtained by FT-IR and XRD analysis. The microstructure of the film was analyzed by SEM, and the properties of the film were tested. The blueberry fresh-keeping test proved that the SDF-added film could effectively prolong the quality of blueberries in storage for 16 days. After 16 days of storage, compared with the control group, the decay rate of the coating group with 0.2% SDF decreased by 16.3%, the consumption of organic acids decreased by 43.7%, and the content of anthocyanin increased by 29.3%. SDF has a potential application in food preservation.Keywords:Jerusalem artichoke;soluble dietary fiber;blueberry
研究领域:
食品科学
·
资源利用
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2020
·Vol. 10
·期 1
·1-10
·
IF 3.998
Ma Huiyan,Chen Yu,Chen Jinhui,Zhang Yunqiu,Zhang Tian,He He
Mikania micrantha and Ipomoea cairica are two invasive plants widely distribute and seriously damage in Hainan island. In this study, the leaves extracts of two weeds were collected and determined for their allelopathic potentials on Chrysanthemum coronarium . The phytotoxicity bioassay showed that when the extract concentration was 50 and 100 mg/ml, the inhibited effects of M. micrantha on growth of C. coronarium were greater than by I. cairica . However, when the extract concertation at 400 mg/ml, the opposite inhibited effects were observed. We speculated this phenomenon was caused by different allelopathic compounds. Therefore, using gas chromatography–mass spectrometry, 19 and 23 compounds were identified respectively, benzoic acid and cinnamic acid were the main components in the two leaves extracts, which were selected to carry out the further bioassays. Subsequent bioassay results showed the effects of two allelochemicals on morphological index and chlorophyll content and POD activity were all negative to C. coronarium , whereas the content of MDA and activity of SOD, CAT represented adverse changes. Moreover, the inhibitions by cinnamic acid were generally greater than those by benzoic acid. Thus, the phenolic acids played the most crucial roles in the allelopathic effccts of M. micrantha and I. cairica leaves extracts.
研究领域:
生态学
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植物学
·
农学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2016
·
IF 2.687
Jin-hua Tao,Jin-ao Duan,Shu Jiang,Jian-ming Guo,Yi-yun Qian,Da-wei Qian
Short-chain fatty acids (SCFAs) produced by the intestinal bacteria are very critical for the intestinal barrier, mucosal cytoprotection and normal intestinal biology. However, accumulation of SCFAs promoted by the polysaccharides from Chrysanthemum morifolium Ramat remains unknown. Thus, it is necessary to investigate SCFAs in the colonic contents of dextran sulfate sodium (DSS) induced colitis mice after oral administration of the polysaccharides from C. morifolium Ramat which is very helpful to unravel how it works. In this study, a rapid and reliable gas chromatographic method with flame ionization detector (GC-FID) for simultaneous determination of six SCFAs such as acetic acid (AA), propionic acid (PA), butyric acid (BA), isobutyric acid (IBA), valeric acid (VA) and isovaleric acid (IVA) has been developed and validated. Under the optimized chromatographic conditions and sample extraction procedure, good separation for 6 target compounds was obtained on a HP-INNOWAX column within 12 min. Results revealed that polysaccharides from C. morifolium Ramat positively affected the SCFAs intestinal production. The polysaccharides group had greater SCFAs concentration in colonic content than the DSS-treated group (P < 0.05), which was decreased remarkably compared to the normal group (P < 0.01). With the decrease of the polysaccharides dosage, the contents of AA, PA and VA increased gradually, while the change of BA concentration was the opposite. There was no significant difference in the content of IBA at the different administration concentrations. And the content of IVA reached the highest concentration 0.953 mg/g at lower dose of the polysaccharides. Additionally, oral administration of the polysaccharides prominently attenuated the body weight loss, reduced the disease activity index, rectal bleeding and stool consistency, improved colon shortening and macroscopic score of colitis. Our results indicated that the polysaccharides of C. morifolium Ramat might be used as prebiotic agents to prevent gut dysbiosis and inflammatory bowel disease.
研究领域:
生物化学
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肠道微生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL SCIENCES
·
2025
·
IF 3.8
Zifan Song,Qing Chang,Yunying Jiang,Xiaodan Qi,Guohui Jiang,Yimin Song
To develop a novel Jerusalem artichoke polysaccharide (JAP)-based delivery system for poorly water-soluble drugs (PWSDs), JAP-SA polymer and florfenicol (FFC)/JAP-SA polymer micelles were prepared. The preparation process was optimized using response surface methodology, with encapsulation efficiency (EE) and drug loading capacity (DLC) as evaluation criteria. Under the optimized conditions [mass ratio of FFC to JAP-SA (1:1), stirring time (5.4 h), and stirring speed (700 r/min)], the EE and DLC reached 69.11 ± 2.04% and 11.99 ± 2.02%, respectively. SEM, TEM, and laser diffraction particle size analysis revealed that the drug-loaded micelles exhibited a unimodal size distribution with an average diameter of 198.33 ± 0.25 nm and a near-spherical morphology. The micelles demonstrated favorable stability, blood compatibility, and cytocompatibility, as assessed through stability testing (STS), erythrocyte hemolysis assay (EHT), and MTT assay. The critical micelle concentration (CMC) of the FFC/JAP-SA micelles, as determined by pyrene fluorescence spectroscopy (PFS), was 19.92 ± 2.01 µg/mL in water and 10.96 ± 1.18 µg/mL in phosphate-buffered saline (PBS). In vitro release studies showed that the FFC/JAP-SA micelles provided sustained drug release in both simulated gastric fluid (SGF, pH 1.2) and simulated intestinal fluid (SIF, pH 6.8), with release kinetics indicating acid-responsive behavior. Additionally, antibacterial efficacy was observed to be concentration-dependent. Pharmacokinetic analysis yielded a time to peak concentration (T max ) of 1.00 ± 0.18 h, a mean residence time (MRT 0-t ) of 8.34 ± 0.68 h, and an elimination half-life (T 1/2β ) of 2.93 ± 2.72 h. These results demonstrate that the FFC/JAP-SA micelles significantly enhanced the aqueous solubility of encapsulated FFC compared to FFC powder, thereby establishing a promising foundation for improving therapeutic efficacy and reducing side effects associated with PWSDs.
研究领域:
药剂学
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高分子材料科学
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药物递送系统
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生物相容性
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药代动力学
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抗菌药理学
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产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2025
·Vol. 14
·期 9
·1109
·
IF 6.6
Yuwen Mu,Bohua Zhang,Shiqi Lv,Fencan Li,Changming Zhao
Jerusalem artichoke ( Helianthus tuberosus L.) is valued for its high inulin content and adaptability to marginal lands. This study investigated the changes in inulin content, antioxidant capacity, polyphenol concentrations, and enzymatic activities of eight cultivars during 60 days of cold storage. Inulin levels ranged from 582.43 g/kg (LZJ006) to 809.70 g/kg (LZJ055), with LZJ047 maintaining the highest content throughout storage. The antioxidant potential, as measured by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, declined across all cultivars, correlating with the reduction in inulin content. The polyphenol content varied significantly, with LZJ119 having 2.17 times more than LZJ010. POD activity increased, while catalase (CAT) and superoxide dismutase (SOD) activities fluctuated during the storage period. Hierarchical Cluster Analysis identified three distinct antioxidant clusters, revealing significant correlations between inulin content and key antioxidant parameters (CAT, FRAP, DPPH). These findings highlight the pivotal role of inulin in preserving the antioxidant system and bioactive properties of Jerusalem artichoke tubers during extended cold storage, providing valuable insights for post-harvest management and cultivar selection.
研究领域:
植物生理学
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采后生物学
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食品科学
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生物化学
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作物遗传育种
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产品关联
DOI
PubMed
高影响因子
TheScientificWorldJOURNAL
·
2014
·
IF 1.219
Fujia Chen,Xiaohua Long,Zhaopu Liu,Hongbo Shao,Ling Liu
Plant phenolics can have applications in pharmaceutical and other industries. To identify and quantify the phenolic compounds in Helianthus tuberosus leaves, qualitative analysis was performed by a reversed phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and quantitative analysis by HPLC. Ten chlorogenic acids (CGAs) were identified (3-o-caffeoylquinic acid, two isomers of caffeoylquinic acid, caffeic acid, p-coumaroyl-quinic acid, feruloylquinic acid, 3,4-dicaffeoyquinic acid, 3,5-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) by comparing their retention times, UV-Vis absorption spectra, and MS/MS spectra with standards. In addition, four other phenolic compounds, including caffeoyl glucopyranose, isorhamnetin glucoside, kaempferol glucuronide, and kaempferol-3-o-glucoside, were tentatively identified in Helianthus tuberosus leaves for the first time. The 3-o-caffeoylquinic acid (7.752 mg/g DW), 4,5-dicaffeoylquinic acid (5.633 mg/g DW), and 3,5-dicaffeoylquinic acid (4.900 mg/g DW) were the major phenolic compounds in leaves of Helianthus tuberosus cultivar NanYu in maturity. The variations in phenolic concentrations and proportions in Helianthus tuberosus leaves were influenced by genotype and plant growth stage. Cultivar NanYu had the highest concentration of phenolic compounds, in particular 3-o-caffeoylquinic acid and 4,5-dicaffeoylquinic acid compared with the other genotypes (wild accession and QingYu). Considering various growth stages, the concentration of total phenolics in cultivar NanYu was higher at flowering stage (5.270 mg/g DW) than at budding and tuber swelling stages. Cultivar NanYu of Helianthus tuberosus is a potential source of natural phenolics that may play an important role in the development of pharmaceuticals.
研究领域:
药理学
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农业化学
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植物科学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Ping Zhu,Yan Zeng,Peng Chen,Yan Men,Jiangang Yang,Xiaoping Yue,Jiangang Zhang,Yueming Zhu,Yuanxia Sun
A one-pot two-enzyme reaction system was developed to produce high-fructose syrup (HFS) containing healthy rare sugar d-allulose from Jerusalem artichoke (JA). Inulin in JA was converted through the cascade reaction of a novel exo-inulinase from Bacillus velezensis (BvInu) and D-allulose 3-epimerase from Ruminococcu s sp. (RDAE). BvInu and RDAE were expressed in Bacillus subtilis and successfully secreted into the supernatant, which decrease the production cost and avoid enzyme purification. The optimal temperature and stability of extracellular BvInu significantly increased, thereby promoting the catalytic activity of the two enzymes for the cascade reaction in one pot. Inulin in JA powder was almost completely converted into monosaccharides in the treatment with the optimal ratio of BvInu : RDAE (80 : 40 U/g inulin) at 50 °C for 2 h. The ratio of D-glucose, D-fructose, and D-allulose in the product was approximately 1:3:1, and the yield on JA powder was 67%. The system exhibits potential high-valued application of non-grain crops on producing HFS with D-allulose.
研究领域:
生物技术
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食品科学
·
酶学
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Ze-Wang Guo,Zi-Fu Ni,Min-Hua Zong,Wen-Yong Lou
Consolidated bioprocessing (CBP) has emerged as a promising strategy for green synthesis of 2,3-butanediol (BDO) from Jerusalem artichoke tubers (JAT). Here, modular metabolic engineering of Bacillus licheniformis MW3 was implemented, in which the overall BDO synthesis process was divided into four modules (inulin hydrolysis, sporulation elimination, carbon flux redistribution, and enantiopure meso-BDO synthesis). First, endo-inulinase was introduced to boost inulin utilization. Second, sporulation was blocked by spoIIE knockout, thus reducing the leakage risk of the genetically engineered strain from the fermentation plant. Third, more carbon flux was shunted into BDO production via exchanging the alsS start codon, blocking acetoin catabolism, and competing pathways. Finally, enantiopure meso-BDO was synthesized by gdh knockout. Encouragingly, the final engineered strain, MWO-8, produced meso-BDO with 99.3% purity at a titer, productivity, and yield of 82.51 g/L, 1.38 g/L/h, and 0.439 g/g, respectively. To our knowledge, this is the highest titer of BDO from JAT by CBP so far. Collectively, this work provides a highly promising alternative approach for sustainable and cost-effective production of BDO.
研究领域:
生物技术
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代谢工程
·
生化工程
·
微生物学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2013
·
IF 2.468
Fujia Chen,Xiaohua Long,Mengni Yu,Zhaopu Liu,Ling Liu,Hongbo Shao
Spoilage of industrial fruits and vegetables in storage and transportation due to fungal infection results in significant losses, and new natural antifungal treatments would have a large economic value. The extracts of antifungal compounds and phenolic acids from Jerusalem artichoke ( Helianthus tuberosus L.) leaves were investigated for potential use in enhancing preservation of fruits and vegetables in storage. Either crude leaf extract or n -butanol fraction was active against Botrytis cinerea, Colletotrichum gloeosporioides, Phytophthora capsici Leonian and Rhizoctonia cerealis , with the values of IC 50 (half of the maximum inhibitory concentration) ranging from 2.166 to 2.534 g L −1 for the crude leaf extract and 0.232–1.911 g L −1 for n -butanol fraction. The severity of grey mould caused by B. cinerea was significantly reduced by n -butanol fraction applied at 1 and 2 g L −1 (the control efficiency of 71.3% and 77.8%, respectively, compared with commercial preparation Carbendazim . Following in vitro activity-guided fractionation by bioautography, six phenolic acids were separated from n -butanol fraction. Among them, caffeic acid, 3,4-dicaffeoylquinic acid and 1,5-dicaffeoylquinic acid played a dominant role and were active in bioassays against Gibberella zeae , with respective minimum inhibitory concentrations (MIC) being 108, 60 and 4.2 μg mL −1 . These results imply that Jerusalem artichoke leaves might be a potential source of natural fungicides.
研究领域:
植物科学
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食品科学
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药理学
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产品关联
DOI
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 4
·2635-2648
·
IF 3.9
Ying Li,Yingxin Tian,Qixue Wang,Xinyi Gu,Long Chen,Yiqun Jia,Shan Cao,Ting Zhang,Mingmei Zhou,Xiaojun Gou
Gynura segetum (Lour.) Merr. (GS), has been widely used in Chinese folk medicine and can promote circulation, relieve pain and remove stasis. In recent years, the hepatotoxicity caused by GS has been reported, however its mechanism is not fully elucidated. Metabolomic techniques are powerful means to explore the toxicological mechanism and therapeutic effects of traditional Chinese medicine. The purpose of this study was to establish a serum metabolomics method based on Gas Chromatography-Mass Spectrometry (GC-MS) to explore the hepatotoxicity mechanism of different exposure times and doses of GS in rats. Sprague Dawley (SD) rats were administered daily with distilled water, 7.5 g kg−1 GS, or 15 g kg−1 GS by intragastrical gavage for either 10 or 21 days. The methods adopted included enzyme-linked immunosorbent assay (ELISA), Hematoxylin and Eosin (H&E) staining and GC-MS-based serum metabolomics. Serum biochemistry analysis showed that the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), total bilirubin (TBIL) and total bile acid (TBA) significantly (P < 0.05) increased while the levels of albumin (ALB) and high-density lipoprotein (HDL) significantly (P < 0.05) decreased in GS-treated groups, compared with the control group. Interestingly, the ALT, AST, TG and ALB levels changed in a time- and dose-dependent manner. The results of H&E staining showed the degree of liver damage after administration of GS gradually deepened with the extension of administration time and the increase of the dose. According to the results of metabolomics analysis, 26 differential metabolites were identified, which were involved in 8 metabolic pathways including phenylalanine metabolism, glyoxylic acid and dicarboxylic acid metabolism and so on. Meanwhile, the number of differential metabolites in different GS-treated groups was associated with GS exposure time and dose. Therefore, we concluded that GS might induce hepatotoxicity depending on the exposure time and dose.
研究领域:
药理学
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毒理学
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代谢组学
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传统中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）