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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2024 · IF 7.7

Liquid culture of Pleurotus nebrodensis mycelium with high yield and extraction and anti-fatigue activity of its polysaccharides

Hua-jin Zeng,Cong-hui Cheng,Si-meng Liu,Yan Ding,Ran Yang,Ling-bo Qu

In this study, the liquid culture system of Pleurotus nebrodensis mycelium with high yield were established by using orthogonal experiments. Results indicated a 58.08 % increase in mycelium biomass and a 2.22 % increase in polysaccharide content after condition optimization. Experiments showed that the extracted polysaccharides have significant antioxidant and anti-exercise fatigue activities. They could effectively scavenge DPPH, ABTS, hydroxyl free radicals and superoxide anaion, prolong weight-loaded swimming time in mice, reduce levels of MDA, LD, and LDH in serum, enhance SOD activity, as well as increase hepatic and muscle glycogen reserves. The mechanism may be attributed to the activation of the Nrf2/Keap1 signaling pathway, inhibition of oxidative stress, and subsequent exerting anti-fatigue effects. The results in this work provides new avenue for easily accessible natural polysaccharide resources with a very convenient approach, which is beneficial for the development of anti-fatigue functional foods for public consumption.

CAS / MDL: 9012-54-8 501-36-0
研究领域: 食品科学 · 微生物学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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FOOD CHEMISTRY · 2011 · IF 3.458

Comparison of sterol composition between Tuber fermentation mycelia and natural fruiting bodies

Yang Tang,Hong-Mei Li,Ya-Jie Tang

Truffle, belonging to Tuber genera, is a nutritious and sterol-rich edible fungus, and sterol is a potential health beneficial compound. A comparison of Tuber sterol composition indicates that the total sterol contents in the fermentation mycelia (i.e., 10.5   mg   g −1 ) ( n   =   3) were approximately 3.2–5.6 times higher than that of the fruiting bodies ( p   <   0.05) with the addition of soybean flour into the basal fermentation media. Moreover, the phytosterol profile of fermentation mycelia could be significantly improved by adding soybean flour into the fermentation media. After the addition of soybean flour, stigmasterol and β-sitosterol appeared in the fermentation mycelia and the contents of total phytosterols (2279   μg   g −1 ) ( n   =   3), brassicasterol (943   μg   g −1 ) ( n   =   3), and campesterol (418   μg   g −1 ) ( n   =   3) were all increased significantly ( p   <   0.05). Moreover, the total contents of sterols and phytosterols in the fermentation mycelia cultured in the soybean media were much higher than those in the fruiting bodies (i.e., 1883–3240 and 479–1832   μg   g −1 , respectively) ( n   =   3, p   <   0.05). This work confirms the potentiality of Tuber fermentation mycelia as the alternative resource for its fruiting bodies from the viewpoint of sterols production.

研究领域: 真菌学 · 营养学 · 食品科学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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KEY ENGINEERING MATERIALS · 2016 · IF 0

Preparation of Gypsum Retarding Material and Activated Carbon from Waste Mycelium

Mi Mi Chen,Meng Meng Zhang,Feng Qing Zhao

A clean and safe process of utilizing waste penicillin mycelium (WPM) was proposed in this paper. We treated WPM by soaking it in aqueous alkaline solution, to produce retarders for hemihydrate gypsum (the protein hydrolysate) by filtration. The filter cake was used to produce activated carbon, while the waste water can be reused for soaking process. This retarder can effectively prolong the setting time of gypsum with less drop in mechanical strength of gypsum plaster. The activated carbon produced with 674 mg/g of Iodine value, which meets the requirements of general industrial applications.

研究领域: 生物工程 · 材料科学 · 环境科学
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Journal of Cleaner Production · 2024 · IF 9.7

Torrefaction: A promising approach for the harmless treatment of low-moisture tetracycline mycelial residue

Enfang Gou,Xuesong Hui,Siqi Song,Huiling Liu,Jie Yao,Xiaohu Dai

Tetracycline mycelial residue (TMR), a byproduct derived from the tetracycline fermentation process, is rich in organic matter, making it a promising resource. However, the presence of residual tetracycline and antibiotic resistance genes (ARGs) limits its potential for resource utilization. In this study, torrefaction was employed for the harmless treatment of low-moisture TMR, and the optimized condition was 200.0 °C for 30.0 min. Under this condition, 97.2% of tetracycline (TC) in TMR could be efficiently removed, with more organic matter reserved for resource utilization. The plausible degradation pathway was proposed, involving the formation of lactone structure and anhydro-tetracycline (ATC) during TC degradation, followed by a series of reactions among main functional groups, including concerted reactions, retro-Diels-Alder (RDA), cleavage reactions, and rearrangement. Furthermore, the antibacterial efficacy of degradation products from TC has significantly diminished, and the structure of ARGs and bacteria was disrupted during the torrefaction of TMR. After the treated TMR was amended into the soil as fertilizer, the dissemination of ARGs could also be effectively controlled. Therefore, torrefaction could be recommended as a promising harmless treatment for the resource utilization of low-moisture TMR.

研究领域: 环境科学 · 微生物学
✓ 已验证 DOI 高影响因子
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JOURNAL OF POWER SOURCES · 2024 · IF 8.1

High performance biochar derived from mycelium-based leather composites waste for energy storage applications

Shenglong Li,Shan Cao,Xintong Li,Yizhi Zhang,Xinde Wang,Xiaojing Zhang,Wenhui Lu,Yang Wang

The mycelial waste generated during the production of mycelium-based leather composites presents both opportunities and challenges in maximizing biomass resource efficiency. In this study, we synthesized mesoporous biochar (AGLM-5) with a specific surface area of 3151.9 m 2 /g through the KOH activation method using Ganoderma lucidum mycelium waste as the precursor material. AGLM-5 exhibited exceptional performance in a three-electrode system test, demonstrating a specific capacitance of 303.5 F/g. In a two-electrode system test, it achieved an energy density of 45.5 Wh/kg at a power density of 450.7 W/kg. The symmetric supercapacitor assembled using AGLM-5 demonstrated outstanding cycling stability over 10,000 cycles, exhibiting a capacitance loss of less than 4 % throughout the entire testing period. Additionally, we employed a hydrothermal method to synthesize composite electrode sheets (δ-MnO 2 /AGLM-5), which not only supported the layered structure of δ-MnO 2 but also significantly improved the cycling stability and electrical conductivity of aqueous zinc ion batteries (AZIB). This study presents an environmentally friendly and sustainable approach for utilizing Ganoderma lucidum mycelial biomass, with particular emphasis on the potential application of mycelial carbon materials in battery energy storage.

研究领域: 材料科学 · 环境科学 · 能源存储
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FOOD HYDROCOLLOIDS · 2023 · IF 10.7

Green and sustainable biomaterials: Edible bioplastic films from mushroom mycelium

Guoqiang Shao,Dan Xu,Zhuojia Xu,Yamei Jin,Fengfeng Wu,Na Yang,Xueming Xu

Scalable mycelium biomaterials with excellent biodegradable and mechanical properties are attracting widespread attention. However, the inherent brittleness of biomass materials greatly limits their applications in reality, such as packaging and flexibility. In this work, three mycelium films were produced by the capacity of mycelium to spontaneously form three-dimensional biopolymer networks with a toughness of 127.32 kJ/m 3 ( Ganoderma lucidum ), 71.95 kJ/m 3 ( Auricularia polytricha ), and 87.52 kJ/m 3 ( Pleurotus ostreatus ), respectively. After glycerol treatment, the toughness of the films increased by more than 440%, and these films’ surfaces were made smooth and hydrophilic. It has been shown that the hydrogen bonding interactions between glycerol and polysaccharides as well as proteins in mycelium enhance the mechanical and hydration properties of mycelial films. The new strategy of using glycerol to improve the properties of mycelium films will advance on mycelium materials research.

CAS / MDL: 56-81-5 7325-16-8
研究领域: 材料科学 · 生物学
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CHEMICAL ENGINEERING JOURNAL · 2021 · IF 13.273

Fungal hypha-derived freestanding porous carbon pad as a high-capacity electrode for water desalination in membrane capacitive deionization

Jiao Chen,Kuichang Zuo,Bing Li,Jiahui Hu,Wenbao Liu,Dongsheng Xia,Lin Lin,Jiajin Liang,Xiao-yan Li

Capacitive deionization (CDI) is an emerging technology for water desalination, especially low-salinity water. Freestanding porous carbon-based electrodes with rapid electron transfer rate and effective ion diffusion features are desired to achieve high desalination performance. Here, a porous carbon pad derived from the fungal hyphae of Aspergillus niger was prepared as a precursor of the electrode. After carbonization and activation, the intertwined and hierarchical porous structure of the fungal hypha pad was well-preserved, producing a freestanding fungal hypha activated carbon pad (FhACPad) electrode for MCDI. The interconnected carbon fibers and open pore structure of the FhACPad yielded an electrode with high electrical conductivity and rapid ion transport properties. The novel FhACPad exhibited a gravimetric salt adsorption capacity of 35.6 ± 2.3 mg NaCl /g with an average desalination rate of 1.2 mg NaCl /g electrode /min from a 585 mg/L NaCl aqueous solution at a cell voltage of 1.2 V and a flow rate of 1 mL/min. As a result, the FhACPad outperformed conventional powdered activated carbon (PAC) electrodes and the most recently reported porous carbon electrodes under similar desalination conditions. This work also provides insights into the structure-performance relationships of CDI electrodes and shows a great potential of fungal biomass-derived porous carbon electrodes for high-performance MCDI applications.

研究领域: 环境科学 · 材料科学 · 生物学
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES · 2024 ·Vol. 25 ·期 21 ·11387 · IF 4.9

Mycelium-Doped Straw Biochars for Antibiotic Control

Bolun Zhang,Ruqi Li,Huiji Zhang,Ye Han,Yunzhe Jia,Siji Chen,Xiaoxiao Yu

Straw, a predominant agricultural residue, represents a significant waste product. Harnessing its potential is of paramount importance both in terms of research and economic value. In this study, chemically pretreated corn straw was infused with distinct microbial fungal mycelium variants and subsequently transformed into a series of biochars through a process involving carbonization and activation. The findings revealed enhancements in the specific surface area and total pore volume of mycelium-doped straw biochars compared to the original corn straw biochar (BCS). Additionally, discernible disparities were observed in their physical and chemical attributes, encompassing functional groups, surface chemistry, and micro-morphology. Notably, in water-based antibiotic removal experiments focusing on tetracycline hydrochloride (TH) and chloramphenicol (CP), the mycelium-doped straw biochars outperformed BCS. Their maximum adsorption capacities for TH and CP surpassed those of alternative adsorbents, including other biochars. Impressively, even after five cycles, the biochar exhibited a removal rate exceeding 80%, attesting to its robust stability. This study successfully emphasized the efficacy of incorporating fungal mycelium to enhance the adsorption properties of straw-based biochar, introducing a new theoretical basis for the development of lignocellulosic materials.

CAS / MDL: 56-75-7 64-75-5
研究领域: 环境科学 · 微生物学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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BIORESOURCE TECHNOLOGY · 2022 · IF 11.889

Preparation of phosphoric acid modified antibiotic mycelial residues biochar: Loading of nano zero-valent iron and promotion on biogas production

Zhifang Ning,Bin Xu,Weizhang Zhong,Chun Liu,Xue Qin,Weibo Feng,Lin Zhu

Antibiotic mycelial residues (AMRs), as recyclable hazardous waste, can realize efficient utilization by reasonable treatment. To solve the problems of undeveloped pore structure and low specific surface area existed in AMR biochar, this study first modified biochar by phosphoric acid (H 3 PO 4 ) to prepare PBC (H 3 PO 4 -modified biochar). Then, PBC was used as carrier to load nano zero-valent iron (nZVI) for preparation of nZVI/PBC. Finally, the biochar materials were used to promote anaerobic digestion (AD) of corn straw. The results showed that H 3 PO 4 -modification can effectively improve the specific surface area, pore structure, and electron donating capacity of AMRs biochar. The using of PBC as carrier to load nZVI attenuated the agglomeration of nZVI particles. Both PBC and nZVI/PBC improved the AD process, with biogas yield enhanced by 29.63% and 29.26%, respectively. The nZVI/PBC exhibited higher ability in maintaining the stability of AD system and promotion of fiber degradation than PBC.

CAS / MDL: 7664-38-2
研究领域: 环境科学 · 微生物学 · 可再生能源
✓ 已验证 产品关联 DOI PubMed 高影响因子
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JOURNAL OF FOOD SCIENCE · 2016 ·Vol. 81 ·期 8 ·C1883 · IF 1.649

Autolysis of Aspergillus oryzae Mycelium and Effect on Volatile Flavor Compounds of Soy Sauce

Ning Xu,Yaqi Liu,Yong Hu,Mengzhou Zhou,Chao Wang,Dongsheng Li

en The autolyzed mycelia of Aspergillus oryzae are rich in proteins, nucleic acids, sugar, and other biomacromolecules, and are one of the main contributors to the flavor profile of commercially important fermented goods, including soy sauce and miso. We induced autolysis of the mycelia of A. oryzae over 1 to 10 d, and found that the maximum dissolved amounts of total protein and nucleic acid ratio accounted for 28.63% and 88.93%, respectively. The organic acid content, such as citric acid, tartaric acid, succinic acid, lactic acid, and acetic acid, initially increased and then decreased as autolysis progressed, corresponding to changes in pH levels. The main characteristic flavor compounds in soy sauce, namely, ethanol, 2-phenylethanol, and 2-methoxy-4-vinylphenol, were all detected in the autolysate. Subsequently, we tested the effect of adding mycelia of A. oryzae during the fermentation process of soy sauce for 60 d, and found that addition of 1.2‰ A. oryzae mycelia provided the richest flavor. Overall, our findings suggest that compounds found in the autolysate of A. oryzae may promote the flavor compounds of soy sauce, such as alcohols, aldehydes, phenols, and esters. Practical Application pt Autolysate of Aspergillus oryzae mycelium contains rich nutrients and volatile flavor compounds, which is an important component of fermentation seasoning. This study provides a theoretical basis for developing the autolysate products of mycelia of A. oryzae and studying the flavor formation of soy sauce. The autolysate of A. oryzae may also be used to prepare healthy drinks and seasoning products.

研究领域: 食品科学 · 微生物学 · 风味化学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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LWT-FOOD SCIENCE AND TECHNOLOGY · 2024 · IF 6

Effect of the mycelium of oyster mushrooms on the physical and flavor properties of a plant-based beef analogue

Zheqi Zhang,Mingwu Zang,Juan Chen,Kaihua Zhang,Shouwei Wang,Dan Li,Xiaoman Li,Meng Liu,Xiaoqian Pan

The influence of mycelium of oyster mushrooms addition on the physical and flavor properties of a fried plant-based beef analogue (PBBA) was evaluated. The key odor compounds of the PBBA were 2-methyl-3-furanthiol, furfuryl mercaptan, ( E ),( E )-2,4-decadienal, and hexanal. The content of volatiles increased with the increase in mycelium content when the mycelium content was <8%. E-nose analysis showed that nitrogen oxides and organic sulfides were the key compounds that contribute to the flavor of samples, and these varied with mycelium content. The umami taste of the PBBA increased slowly with the mycelium content; e-tongue and sensory evaluation showed that the mycelium content <8% may inhibit bitterness and astringency. In addition, mycelium can increase the springiness, brightness, and whiteness of the PBBA and reduce the redness and beany flavor. Overall, the addition of a moderate amount of mycelium can enhance the meat-like and fat-like aroma of PBBA, reduce some undesirable odor and taste, and made it closer to meat. These findings demonstrate the potential of mycelium as a raw material for PBBAs.

研究领域: 食品科学 · 农业 · 化学
✓ 已验证 DOI 高影响因子
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CHEMICAL ENGINEERING JOURNAL · 2014 · IF 4.058

A novel biosorbent formed of marine-derived Penicillium janthinellum mycelial pellets for removing dyes from dye-containing wastewater

Ming-Xia Wang,Qi-Lei Zhang,Shan-Jing Yao

Mycelial pellets prepared using a marine-derived Penicillium janthinellum sp. strain (P1) were applied as biosorbents to remove dyes from aqueous solutions. Nine different dyes were studied and the effects of pH and salt concentration on the decolorization processes were investigated. The results showed that the mycelial pellet was effective in removing dyes from their water solutions. Moreover, it was found that pH was an important factor of the decolorization process and the best dye removal performance was achieved when pH   =   5.0. The adsorption process of the live mycelial pellets was not affected by high salt concentrations. In addition, the mycelial pellets retained 91.3% of the original decolorization activity after five reusing cycles without any desorption treatments. The adsorption isotherms indicated that the biosorption process agreed well with the Langmuir isotherm model with a maximum biosorption capacity of 344.83   mg   g(dry) − 1 and the kinetic adsorption results showed that the biosorption process followed a pseudo-second-order kinetic model.

研究领域: 环境微生物学 · 环境工程 · 生物吸附 · 水污染控制 · 应用微生物学 · 环境化学
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