Stress Biology
·
2023
·Vol. 3
·期 1
·1-12
·
IF 0
Fei Yu-chen,Cheng Qin,Zhang Huan,Han Chuang,Wang Xu,Li Yan-feng,Li Shi-qian,Zhao Xiao-hu
Sclerotinia sclerotiorum is a necrotrophic plant pathogenic fungus with broad distribution and host range. Bioactive compounds derived from plant extracts have been proven to be effective in controlling S. sclerotiorum . In this study, the mycelial growth of S. sclerotiorum was effectively inhibited by maleic acid, malonic acid, and their combination at a concentration of 2 mg/mL, with respective inhibition rates of 32.5%, 9.98%, and 67.6%. The treatment of detached leaves with the two acids resulted in a decrease in lesion diameters. Interestingly, maleic acid and malonic acid decreased the number of sclerotia while simultaneously increasing their weight. The two acids also disrupted the cell structure of sclerotia, leading to sheet-like electron-thin regions. On a molecular level, maleic acid reduced oxalic acid secretion, upregulated the expression of Ss-Odc2 and downregulated CWDE10 , Ss-Bi1 and Ss-Ggt1 . Differently, malonic acid downregulated CWDE2 and Ss-Odc1 . These findings verified that maleic acid and malonic acid could effectively inhibit S. sclerotiorum , providing promising evidence for the development of an environmentally friendly biocontrol agent.
研究领域:
植物病理学
·
生物化学
✓ 已验证
DOI
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Hua-jin Zeng,Cong-hui Cheng,Si-meng Liu,Yan Ding,Ran Yang,Ling-bo Qu
In this study, the liquid culture system of Pleurotus nebrodensis mycelium with high yield were established by using orthogonal experiments. Results indicated a 58.08 % increase in mycelium biomass and a 2.22 % increase in polysaccharide content after condition optimization. Experiments showed that the extracted polysaccharides have significant antioxidant and anti-exercise fatigue activities. They could effectively scavenge DPPH, ABTS, hydroxyl free radicals and superoxide anaion, prolong weight-loaded swimming time in mice, reduce levels of MDA, LD, and LDH in serum, enhance SOD activity, as well as increase hepatic and muscle glycogen reserves. The mechanism may be attributed to the activation of the Nrf2/Keap1 signaling pathway, inhibition of oxidative stress, and subsequent exerting anti-fatigue effects. The results in this work provides new avenue for easily accessible natural polysaccharide resources with a very convenient approach, which is beneficial for the development of anti-fatigue functional foods for public consumption.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 10
·1443
·
IF 5.561
Hongbo Li,Suya Xie,Shangqiao Cao,Liangbin Hu,Dan Xu,Jiayi Zhang,Haizhen Mo,Zhenbin Liu
Edible fungi play an important role in material and energy cycling. This study explored the role ofPleurotus eryngiimycelia in the transformation of potato high-calorie starch to low-calorie β-glucan. First, the 3D printing performance of the potato medium was optimized. After inoculating the fermentation broth ofPleurotus eryngiiin 3D printing, we studied the microstructure and material composition of the product. Along with the increase in 3D printing filling ratio, the starch content of the culture product decreased from 84.18% to 60.35%, while the starch content in the solid medium prepared using the mold was 67.74%. The change in β-glucan content in cultured products was opposite to that of starch, and the content of the culture product increased from 12.57% to 24.31%, while the β-glucan content in the solid medium prepared using the mold was 22.17%. The amino acid composition and content of the 3D printing culture system and solid culture products prepared using the mold were similar. The 3D printing culture system promoted the bioconversion efficiency of mycelia. It also showed high application potential ofPleurotus eryngiimycelia for the preparation of low-calorie food.Keywords:bioconversion;Pleurotus eryngiimycelia;starch;3D printing;β-glucan
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 20
·7997-8007
·
IF 4.813
Shu Lili,Zeng Zhiheng,Dai Jianqing,Cheng Yi,Lu Yuanping,Chen Meiyuan,Zeng Hui
Agaricus bisporus is the most widely cultivated edible mushroom in the world. Strain quality has an important influence on the yield of A. bisporus , with strains that exhibit aging being a common problem during cultivation. However, little is known about the aging mechanisms of A. bisporus strain. In this study, the normal A. bisporus As2796 strain was compared to the aging A. bisporus As2796Y strain (which was previously discovered during cultivation). In the aging As2796Y mycelia, the mycelial growth rate and fruiting body yield were decreased and the chitin level and cell wall thickness were increased. Additionally, intracellular vacuoles increased, there was cytoplasmic shrinkage, and the sterol level which stabilizes the cell membrane decreased, which led to cytoplasmic outflow and the exudation of a large amount of yellow water from the mycelia. Additionally, there was increased electrolyte leakage. Furthermore, gas chromatography-mass spectrometry (GC/MS) was used to profile the metabolic changes in the aging As2796Y mycelia compared to the normal As2796 mycelia. A total of 52 differential metabolites were identified (75% were downregulated and 25% were upregulated in As2796Y). The reduction of many metabolites decreased the mycelial viability and the ability to maintain cell stability. Overall, this study is the first to report on the morphologic and metabolic changes in aged A. bisporus mycelia, which will aid future research on the mechanisms underlying A. bisporus mycelial aging.
Key points
• Aging of Agaricus bisporus strains will greatly reduce the fruiting body yield. • Aging of Agaricus bisporus strains can significantly change the cell structure of mycelia. • Many metabolites in the mycelium of aging spawn As2796Y significantly changed.
研究领域:
真菌学
·
代谢组学
·
植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2011
·
IF 3.458
Yang Tang,Hong-Mei Li,Ya-Jie Tang
Truffle, belonging to Tuber genera, is a nutritious and sterol-rich edible fungus, and sterol is a potential health beneficial compound. A comparison of Tuber sterol composition indicates that the total sterol contents in the fermentation mycelia (i.e., 10.5 mg g −1 ) ( n = 3) were approximately 3.2–5.6 times higher than that of the fruiting bodies ( p < 0.05) with the addition of soybean flour into the basal fermentation media. Moreover, the phytosterol profile of fermentation mycelia could be significantly improved by adding soybean flour into the fermentation media. After the addition of soybean flour, stigmasterol and β-sitosterol appeared in the fermentation mycelia and the contents of total phytosterols (2279 μg g −1 ) ( n = 3), brassicasterol (943 μg g −1 ) ( n = 3), and campesterol (418 μg g −1 ) ( n = 3) were all increased significantly ( p < 0.05). Moreover, the total contents of sterols and phytosterols in the fermentation mycelia cultured in the soybean media were much higher than those in the fruiting bodies (i.e., 1883–3240 and 479–1832 μg g −1 , respectively) ( n = 3, p < 0.05). This work confirms the potentiality of Tuber fermentation mycelia as the alternative resource for its fruiting bodies from the viewpoint of sterols production.
研究领域:
真菌学
·
营养学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 44
·25480-25487
·
IF 3.049
Siji Chen,Zhixiao Wang,Yuhan Xia,Bolun Zhang,Huan Chen,Guang Chen,Shanshan Tang
In this work, fungal hyphae (FH, Irpex lacteus) was used as the carbon resource for the preparation of porous carbon materials (PCFH) using mixed alkali as the activator. The SEM, N2 adsorption/desorption, FT-IR, XRD, Raman, and XPS were used to characterize the structure and surface properties of PCFH. The results showed that the PCFH not only has a huge Brunauer–Emmett–Teller (BET) surface area (2480 m2 g−1), but also has abundant functional groups containing carbon, oxygen, and nitrogen. Rhodamine B (RhB) was selected to evaluate the adsorption properties of the PCFH prepared under different conditions in dyeing wastewater. A fast adsorption rate was observed, and an uptake capacity of 765 mg g−1 was achieved in the initial 5 min. The maximum adsorption capacity of PCFH to RhB reached 1912 mg g−1 at the pH value of 9, which could efficiently remove RhB from the aqueous solution. The adsorption process was fitted better by a pseudo-second order model, and the adsorption isotherm for the RhB was well fitted by the Freundlich model. Moreover, the probable mechanism of adsorption was analyzed. In short, the good adsorption performance of PCFH indicated that it has a broad application prospect for dye water pollution control.
研究领域:
环境科学
·
材料科学
·
生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Chen Xiao,Zhang Zheng,Liu Xiaoxia,Cui Bo,Miao Wentao,Cheng Weiwei,Zhao Fengyun
Volvariella volvacea is a typical edible Basidiomycete with a high-temperature tolerance. It has a strong fibrinolysis capability and consumes abundant agricultural wastes. In agricultural cultivation, mycelial subculturing has been adopted, leading to serious strain degeneration. In this study, continuous mycelial subculturing of the common V. volvacea strain V971 (original strain recorded as M0) was performed in potato dextrose agar (PDA) medium. One generation of the strain was preserved every 3 months (90 days); thus, six generations of degenerated strains (M1–M6) were obtained after 18 months of mycelial subculturing. The original and degenerated strains were preserved in sterile paraffin liquid at room temperature (18–25°C). The biological traits and nutrients of M0 and M1–M6 were studied. The mycelial growth rate and biomass initially increased and then decreased as the degeneration progressed, reaching minimum levels of 0.041 ± 0.001 cm/h and 1.82 ± 0.25 g, respectively, at M6. Additionally, the polysaccharide, protein, polyphenol, flavone, total amino acid, and total mineral element contents of the strains decreased continuously, reaching minimum levels of 30.12 ± 3.12 g/100 g, 26.42 ± 2.1 g/100 g, 1.08 ± 0.05 g/100 g, 4.23 ± 0.21 g/100 g, 12.51 mg/g, and 398.05 mg/kg, respectively, at M6. The decolorization capability of V. volvacea in liquid medium supplemented with bromothymol blue and lactose reflected the degree of strain degeneration, with the capability weakening as the degeneration intensified. These results are highly significant for V. volvacea production. The mycelial characteristics during subculture-associated degeneration were described and provide an early identification method for V. volvacea’s degeneration.
研究领域:
真菌学
·
食用菌研究
·
农业微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Junhua Kuang,Shuaishuai Zhang,Jia Yu,Yuting Zhang,Chun-Kuo Peng,Chen Zou,Jiaran Li,Li Peng,Lu Lin,Yan-Gu Lin,Pengbo Lyu,Shuliang Yang,Jian-Feng Li
Iron, one of the most abundant elements on earth and an essential element for living organisms, plays a crucial role in our daily metabolism. In the field of catalysis, the development of high-performance catalysts based on less toxic iron element is also of significant importance for green chemistry and a sustainable future. To construct Fe-based heterogeneous catalysts with excellent hydrogenation performance, precise modulation of the atomic coordination structure is a key strategy for enhancing catalytic activity. In this study, we present an in-situ coating method for applying a zeolitic imidazolate framework (ZIF) onto the surface of fungal hyphae. The asymmetric coordination structure of Fe 1 -N 3 P 1 was precisely tailored by utilizing the phosphorus source from the fungus and the nitrogen source in the ZIFs. Detailed characterizations and density functional theory calculations revealed that the incorporation of ZIFs not only increased the specific surface area of catalysts, but also facilitated the dispersion of Fe 2 P nanoparticles into the Fe 1 -N 3 P 1 center, making the lowest reaction energy barrier and resulting in the best performance for nitrobenzene hydrogenation when compared to the Fe 2 P nanoparticles and clusters. This research introduces a novel design concept for constructing asymmetric monoatomic configuration based on the inherent characteristics of natural microorganisms and the exogenous porous coordination polymers.
研究领域:
催化化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2024
·
IF 2.9
Chen Huajiang,Luo Yanran,Xing Guiqing,Shi Yongqing,Gu Shuang,Wang Xiangyang
Aspergillus spp. are common dominant fungi in the moldy agricultural products. Different Aspergillus species exhibit high morphological and genetic sequence similarity, making identification challenging. The measurement of mycelial growth in these Aspergillus species often requires a relatively long time. Therefore, there is a need to develop a new rapid method for identifying Aspergillus species and monitoring their growth. The volatile compounds released by five Aspergillus species on culture media were detected by gas chromatography-mass spectrometer (GC–MS), electronic nose and headspace-gas chromatography-ion migration spectrometry (HS-GC-IMS), their characteristic volatile signals were captured, the models for identifying Aspergillus species were established, and prediction models for mycelial growth of five Aspergillus species were developed. The results showed that the LDA classification training set model constructed using the fusion of GC-IMS and electronic nose data achieved a 100% accuracy rate for identifying Aspergillus species in the training set and an 83.3% accuracy rate in the test set, significantly outperforming models based on HS-GC-IMS or electronic nose data alone. When the result combined with morphological analysis, a 100% accuracy rate in identifying Aspergillus species could be achieved. The partial least squares regression (PLSR) prediction models for mycelial growth in Aspergillus species, constructed using both fusion electronic nose data and HS-GC-IMS data, demonstrated high accuracy with prediction accuracies greater than 0.972 for all five species. The prediction model based solely on electronic nose data had lower accuracy, while models using HS-GC-IMS data alone or its combined with electronic nose data achieved good results. This fusion approach of HS-GC-IMS and electronic nose might have the potential to serve as a preliminary screening tool for fungi identification.
研究领域:
微生物学
·
食品科学
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Man Xu,Xue Luo,Xin-Li Yan,Xiao-Yu Cai,Lin-Lin Wang,Feng Ge,Hong-Wei Wang
Etoxazole is a widely used insecticide that poses a serious threat to both ecosystems and human health. In present study, a novel strain Paracoccus versutus Y4 was isolated and identified. More than 98 % of the etoxazole (10 mg/L) was degraded as the sole carbon source within 8 d by strain Y4 in liquid culture. HPLC MS/MS analysis revealed three possible intermediates, and a novel metabolic pathway of etoxazole including oxidation, dehydrogenation, and hydrolysis reactions was proposed. The Toxicity Estimation Software Tool suggests that the biodegradation intermediates were less harmful than etoxazole. Whole-genome sequencing revealed that the genome size of P. versutus Y4 was 5320,902 bp containing 5187 coding sequences. Among them, the gene coding monooxygenase, dehydrogenase and hydrolase may be responsible for etoxazole biodegradation. The results of molecular docking analysis suggested that the monooxygenase, dehydrogenase, and hydrolase from strain Y4 may facilitate catalytic degradation through efficient substrate binding. Compared with diatomite carrier, fungal mycelium carrier can promote the growth of strain Y4. In the soil degradation experiments, the fungal mycelium carrier promoted etoxazole degradation by strain Y4 in both fresh and sterilized soil. Treatment with Y4 +fungal mycelium significantly reduced the half-life of etoxazole in fresh soil from 24.2 to 6.3 d. Our study is the first to isolate etoxazole-degrading bacteria and provides a new strategy for the bioremediation of pesticide pollution by combining degrading microbes and fungal mycelium carriers.
研究领域:
环境科学
·
微生物学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Jiao Chen,Jiahui Hu,Kuichang Zuo,Xiao-yan Li,Liyuan Zhang
Selective adsorption is of high value in many important areas, but this is a well-known challenge. Here, a self-supporting carbon film electrode, used as anode for selective adsorption of NO 3 – , was fabricated successfully by using fungus hyphae as feedstock. The removal of NO 3 – by the carbon film electrode is 0.345 m mol/g anode , accounting for 85.2 % of the total removed anions. The selectivity of NO 3 – over Cl - and NO 3 – over SO 4 2- is 6.7 and 13.8, respectively. The preferential electrosorption is relevant to the narrow micropores structure and the nitrogen doping of the carbon film, based on the pore size analysis and density functional calculation. This work opens a hopeful window in providing an effective approach to fabricate electrosorption materials and also could inspire the future development of selective materials for electrosorption of target ions.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 27
·2212570
·
IF 19
Xiao Fu,Shuai Zhang,Xu Zhang,Yan Zhang,Baoxi Li,Keda Jin,Xingwei Feng,Juan Hong,Xiang Huang,Hongliang Cao,Qiaoxia Yuan,Ping Ai,Hongbo Yu,Qiang Li
Plastic-induced pollution has recently triggered global environmental, biodiversity, and public health concerns. Plastic micro/nanoparticles suspended in water that are non-recyclable and non-degradable are found in plants, animals, and even human blood, and their remediation represents an emergent societal need. In this study, a highly efficient strategy is reported to remove microplastics by using a sustainable framework derived from fungal mycelium (FM), which has reached a record capacity at 2.49 g g −1 , as it is known. This excellent removal capacity results from both the inherent properties and surface cationization of the FM. First, FM has a loose entanglement and porous structure with extracellular polymeric substances on the surface, which endows FM with the capacity to adsorb microplastics. Second, FM is engineered with 2,3-epoxypropyltrimethylammonium chloride (EPTAC) to enable its positively charged surface, which significantly enhances the adsorption of microplastics. Kinetic analysis and density functional theory reveal that the excellent microplastic removal is attributed to the enhanced electrostatic interaction between microplastics and EPTAC- g -FM. Along with the inherent merits of FM, which are natural, renewable, biodegradable, environmentally friendly, and easy to scale up, FM represents a green, facile, and cost-effective next-generation technology for remediating microplastics in clean water.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）