PEST MANAGEMENT SCIENCE
·
2020
·Vol. 76
·期 9
·2978-2985
·
IF 3.75
Xianfei Zhang,Jiuyong Xu,Solange Muhayimana,Hui Xiong,Xuefeng Liu,Qingchun Huang
BACKGROUND Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum threatens oilseed rape cultivation, and the emergence of fungicide-resistant strains has led to control failures worldwide. Identifying novel chemical alternatives with different modes of action and high antifungal activities is thus crucial. Herein we evaluated the antifungal effects of 3-(2-pyridyl)methyl-2-(4-chlorphenyl)imino- thiazolidine (PMAS) on S. sclerotiorum to determine its efficacy for SSR management. RESULTS PMAS had an inhibitory effect on mycelial growth; the EC 50 values were 17.83 and 21.15 μg mL −1 for the carbendazim-susceptible strain Ss01 and carbendazim-resistant strain Hm25, respectively. PMAS treatment changed the color of inhibited mycelia to green, and the hyphae were sustained in the undifferentiated stage. Cysteine supplementation made this green color disappear, whereas methionine enhanced the color. Moreover, PMAS treatment markedly inhibited oxalic acid biogenesis, increased free thiol content in mycelia, and weakened the activities of oxaloacetase and malate dehydrogenase, but had little effect on the activity of glyoxylate dehydrogenase. Cysteine could reverse the inhibitory effects of PMAS on mycelial morphogenesis and biochemical constituents, except thiol production. In the pot-culture experiment, PMAS showed a good protective effect, with the control efficacy being >91% on SSR. CONCLUSION PMAS appears to be an effective fungicide for SSR management. © 2020 Society of Chemical Industry
研究领域:
植物病理学
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农学
·
分子生物学
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Xuan Wang,Caicheng Long,Xiaoyan Xu,Taiping Qing,Peng Zhang,Bo Feng
Micrococcal nuclease (MNase) is a nonspecific endo-exonuclease that digests single-stranded/double-stranded DNA and RNA. The existence of MNase can serves as an important diagnostic biomarker of Staphylococcus aureus ( S. aureus ) infection. However, most of the substrates in MNase-based sensors are single-stranded DNA, which could also be digested by exonuclease I or S1 nuclease and interfere the MNase detection. In this work, we developed a highly selective fluorescent method for MNase detection using a specific dsDNA and nucleic acid dye SYBR Green I (SGI) as the indicator. After rational design, an AT-rich dsDNA with 3′ protruding termini was screened as the high-specific substrate of MNase assay and efficient enhancer of SGI. The AT-rich dsDNA substrate can resist the digestion of other exonuclease and greatly enhance the fluorescence of SGI. This high-specific substrate-based probe can detect MNase in buffer as well as biological sample with highly selectivity . Moreover, this method was also applied to monitor the MNase secreted by S. aureus . Thus, the proposed MNase-based assay has a strong potential to identify S. aureus in food safety and microbial infection due to its excellent analytical sensitivity and high selectivity.
研究领域:
微生物学
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分子生物学
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医学诊断
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
·
IF 5.401
Dongxia Li,Jin Qin,Guiqin Yan
To solve interference from background fluorescence and scattering light of real biological samples, we developed a room-temperature phosphorescence (RTP) sensor to detect Micrococcal nuclease (MNase). The sensor was based on the phosphorescent resonance energy transfer (PRET) between 3-mercaptopropionic acid (MPA)-capped Mn-doped ZnS quantum dots (QDs) and single-marked oligonucleotide (DNA-ROX). Specifically, the poly-(diallyldimethylammonium chloride) (PDAD)-modified QDs (PDAD-QDs) were prepared as the energy donor, and the DNA-ROX was chosen as the energy receptor. The DNA-ROX could be adsorbed to the surface of the PDAD-QDs through electrostatic interaction, which induced PRET and quenched the RTP of PDAD-QDs. The MNase could efficiently degrade the DNA-ROX into small DNA segments, which were less prone to electrostatic interaction with PDAD-QDs, and thus the PRET efficiency decreased. The RTP intensity of PDAD-QDs was gradually enhanced with the increment of MNase concentration. Under the optimal conditions, the change of RTP intensity was proportional to the logarithm of MNase concentration in the range from 2 × 10 −3 to 8.0 × 10 −2 U mL −1 , with a high correlation coefficient of 0.993 and a detection limit of 6 × 10 −4 U mL −1 . This proposed RTP sensor can avoid interferences from the background fluorescence or scattering light of the matrix that are encountered in spectrofluorometry. Thus, this biosensor can be applied to detect MNase in culture media.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Bochi Yu,Yanli Man,Pingping Wang,Chi Wu,Jun Xie,Wei Wang,Hongyun Jiang,Lan Zhang,Yanning Zhang,Liangang Mao,Lizhen Zhu,Yongquan Zheng,Xingang Liu
N-doped biochar is widely used for activating persulfate to degrade organic pollutants. Which type of N atom is the key factor for activation is still unclear and needs to be further explored and analyzed. In this study, four kinds of biochar were prepared using urea and rice husk as precursors, and tested for the catalytic degradation of dimethomorph. Increasing the nitrogen doping level caused the catalytic removal efficiency of dimethomorph in the presence of peroxymonosulfate increased from 16.6% to 86.8%. A correlation analysis showed that the ability of N-doped biochar to activate PMS is mainly related to the content of pyrrole N, graphite N and carbonyl and the degree of defects. In experiments on electron paramagnetic resonance and free radical suppression, the reactive species of SO 4 •- , 1 O 2 ,·OH and O 2 .− were detected, among which 1 O 2 was found to be the main agent in the nonradical pathway. The degradation pathways for dimethomorph were analyzed based on a total of 8 degradation products identified by high-performance liquid chromatography-time of flight mass spectrometry (HPLC-Q-TOFMS). The results of this study provide a fundamental basis for using agricultural waste to produce inexpensive and efficient nonmetal catalysts that are highly effective in reducing dimethomorph levels in agricultural lands.
研究领域:
环境科学
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农业科学
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DOI
PubMed
高影响因子
JOURNAL OF PEST SCIENCE
·
2012
·Vol. 86
·期 2
·353-360
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IF 1.509
Shen Zhenzhong,Fan Yongxian,Li Fuge,Chen Xiaolong,Shen Yinchu
Compounds with maleimide, both natural and synthesized, have good biological activities, especially the antifungal activity. In order to investigate the antifungal activity of dimethylmaleimides, 17 N -substituted dimethylmaleimides were prepared from the reactions of 2,3-dimethyl maleic anhydride and amines using a facile synthetic method in this paper. These compounds were evaluated for antifungal activities against Sclerotinia sclerotiorum by the mycelium growth rate method. They exhibited minimum inhibitory concentrations (MICs) ranging from 0.01–50.0 μg/mL, with N -(2-benzimidazole)-3,4-dimethylmaleimide being the most active one with an MIC of 0.01 μg/mL. The structure and activity relationship on these compounds indicated that the hydrophobicity of the N -substituents is associated with their antifungal activity. Compared to current antifungals, most of N -substituted dimethylmaleimides have a perfect activity for S. sclerotiorum control and low toxicity.
研究领域:
植物病理学
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有机化学
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农药学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Long Yang,Hui Zhao,Yucong Li,Yanqiong Zhang,Hanzhang Ye,Genfu Zhao,Xin Ran,Feng Liu,Can-Peng Li
In this work, the molecular recognition of dimethomorph by disulfide bridged β–cyclodextrin (SS–β–CD) was studied by UV spectroscopy , 2 D NMR, and molecular modeling . The results indicated that the SS–β–CD/dimethomorph was more stable than β–CD/dimethomorph, which is ascribed to the fact that the disulfide chain plays an important role in stabilizing the appropriate dual-CD conformation and also promoting the inclusion of the host and guest. In addition, a robust fluorescence method for dimethomorph sensing has been developed based on competitive host–guest interaction by selecting safranine T (ST) as optical indicator and SS–β–CD functionalized reduced graphene oxide (SS–β–CD–RGO) as the receptor. Upon the presence of dimethomorph to the pre-formed SS–β–CD–RGO·ST complex, the ST molecule is displaced by dimethomorph, leading to a “switch–on” fluorescence response. That is due to the fact that the binding constant of the dimethomorph/SS–β–CD complex was more than 5 times greater than that of ST/SS–β–CD. The fluorescence intensity of SS–β–CD–RGO·ST complex increased linearly with increasing concentration of dimethomorph ranging from 0.50 to 20.0 μM. The proposed method showed a detection limit of 0.11 μM for dimethomorph, and was successfully applied for the determination of dimethomorph residues in vegetables (cabbage, spinach) and environmental samples (water, soil) with good precision and recoveries from 96.5% to 104%.
研究领域:
化学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Tao Tang,Fanfan Wang,Houyun Huang,Nengneng Xie,Jie Guo,Xiaoliang Guo,Yuanyuan Duan,Xiaoyue Wang,Qingfang Wang,Jingmao You
This study explores the antipathogenic properties of volatile organic compounds (VOCs) produced by Bacillus velezensis LT1, isolated from the rhizosphere soil of Coptis chinensis. The impact of these VOCs on the mycelial growth of Sclerotium rolfsii LC1, the causative agent of southern blight in C. chinensis, was evaluated using a double Petri-dish assay. The biocontrol efficacy of these VOCs was further assessed through leaf inoculation and pot experiments. Antifungal VOCs were collected using headspace solid-phase microextraction (SPME), and their components were identified via gas chromatography–mass spectrometry (GC–MS). The results revealed that the VOCs significantly inhibited the mycelial growth and sclerotia germination of S. rolfsii LC1 and disrupted the morphological integrity of fungal mycelia. Under the influence of these VOCs, genes associated with chitin synthesis were upregulated, while those related to cell wall degrading enzymes were downregulated. Notably, 2-dodecanone and 2-undecanone exhibited inhibition rates of 81.67% and 80.08%, respectively. This research provides a novel approach for the prevention and management of southern blight in C. chinensis, highlighting the potential of microbial VOCs in biocontrol strategies.
研究领域:
植物病理学
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微生物学
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DOI
PubMed
高影响因子
mSphere
·
2024
·
IF 3.7
Chen Tang,Haifeng Wang,Xianjiang Jin,Wenwen Li,Yonglin Wang
Verticillium dahliae causes Verticillium wilt in more than 200 plant species worldwide. As a soilborne fungus, it forms melanized microsclerotia and colonizes the xylem of host plants. Our previous study revealed a subfamily of C2H2-homeobox transcription factors in V. dahliae, but their biological roles remain unknown. In this study, we systematically characterized the functions of seven C2H2-homeobox transcription factors in V. dahliae. Deletion of VdChtf3 and VdChtf6 significantly decreased the production of melanized microsclerotia, and knockout of VdChtf1 and VdChtf4 enhanced virulence. Loss of VdChtf2 and VdChtf6 increased conidium production, whereas loss of VdChtf5 and VdChtf7 did not affect growth, conidiation, microsclerotial formation, or virulence. Further research showed that VdChtf3 activated the expression of genes encoding pectic enzymes to participate in microsclerotial formation. In addition, VdChtf4 reduced the expression of VdSOD1 to disturb the scavenging of superoxide radicals but induced the expression of genes related to cell wall synthesis to maintain cell wall integrity. These findings highlight the diverse roles of different members of the C2H2-homeobox gene family in V. dahliae.
研究领域:
真菌学
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植物病理学
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转录调控
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DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
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2018
·
IF 2.975
Chenyun Dong,Wanling You,Ruqi Liuyang,Yufeng Lei,Anqiang Zhang,Yaling Lin
Polymeric quaternary ammonium salts (PQASs) exhibit antibacterial action and are less toxic, less stimulatory to the human body and have easier-to-modify functionalities than small molecular antibacterial agents . However, few studies on the structure-activity relationship and toxicity mechanism of PQASs against fungi have been reported. We previously described the synthesis of a novel PQAS, namely, a homopolymer of (2-methacrylamido)propyltetrabenzyldimethylammonium chloride (PQD-BC), and discovered that the polymer exhibits antifungal activities not only against Fusarium oxysporum f. sp. Cubense tropical race 4 (Foc 4), the pathogen of banana wilt, but also against Rhizoctonia solani ( R. solani ), the pathogen of rice sheath blight (RShB). Furthermore, we studied the mechanism of action of PQD-BC against Foc 4, which is markedly different from R. solani in morphology and life cycle. Therefore, the structure-antifungal activity relationship and toxicity mechanism of PQD-BC against R. solani were extensively studied in this work and compared with those of the low-molecular-weight quaternary ammonium salt benzyldimethyldodecylammonium chloride (BC), and the results play an important role in identifying long-term and low-toxicity fungicides that can suppress the sclerotia of R. solani . The results showed that PQD-BC and BC can destroy the structural integrity and morphology of a cell, such as by loss of the cell wall and plasma membrane integrity, leading to the release of intracellular contents and can induce mitochondrial dysfunction and interference with genomic DNA and inhibit the formation of sclerotia. However, PQD-BC showed a special mechanism for causing the lipid peroxidation of the cell membrane; this mechanism was not observed with BC. The newly elucidated mechanism accounts for differences between polymers and small-molecule compounds and provides a theoretical basis for further application of PQAS against fungi and sclerotia.
研究领域:
植物病理学
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真菌学
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药物化学
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生物技术
✓ 已验证
DOI
高影响因子
Current Protocols
·
2025
·Vol. 5
·期 7
·e70179
·
IF 2.2
Bingyan Yuan,Jingjun Hong
In eukaryotic cells, nucleosomes are octameric structures composed of DNA and histones, and their tandem arrangement ultimately forms chromosomes. Nucleosome assembly is governed in vivo by a series of molecular mechanisms, particularly histone chaperones such as Swr1, which is indispensable for H2A.Z-nucleosome formation. The assessment of nucleosome assembly using micrococcal nuclease (MNase) digestion has been widely accepted. We describe a simple and reproducible protocol for analyzing the chaperone activity of SRCAP (Snf2-related CREBBP activator protein), the histone H2A.Z chaperone. This approach involves purifying a complex of H2A.Z, H2B, and SRCAP via fast protein liquid chromatography (FPLC) only, followed by its assembly with DNA-(H3.1-H4 A15C ) 2 . The formation of nucleosomes is then determined in vitro using electrophoretic mobility shift assay (EMSA) and MNase digestion. This approach can also be adapted to evaluate whether other histone chaperones or regulatory factors promote nucleosome assembly activity. © 2025 Wiley Periodicals LLC. Basic Protocol : In vitro nucleosome assembly facilitated by the histone chaperone SRCAP
研究领域:
分子生物学
·
生物化学
·
细胞生物学
·
表观遗传学
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Zhang Shujing,Liu Qi,Han Yunfei,Han Jinghua,Yan Zhiqiang,Wang Yonghong,Zhang Xing
This study was conducted to purify and identify metabolites of antimicrobial activity against phytopathogens from Xenorhabdus nematophila YL001. Three dipeptide compounds were purified from its cell-free cultural broth and identified as (±)-nematophin, cyclo (L-Pro-Gly), and N, N′-dimethyl-cyclo (L-Phe-L-Leu). Nematophin demonstrated a wider antifungal spectrum than the other two compounds. It also exhibited strong inhibitory effects on mycelial growth of Rhizoctonia solani and Phytophthora infestans with EC50 values of 40.00 and 51.25 μg/ml, respectively. Its (S)-configuration structure [(+)-nematophin] was also synthesized and exhibited higher antimicrobial activity than the enantiomeric mixture. The detached leaf assay revealed that nematophin possessed significant preventive and curative efficacy against R. solani on broad bean leaves showing corresponding control efficacies of 93.01 and 94.93% at 1,000 μg/ml, comparable to those of a chemical fungicide (carbendazim) at 500 μg/ml. Additionally, the pot experiments indicated that nematophin could effectively inhibit the disease extension on rice and broad bean plants caused by R. solani. Nematophin also exerted some adverse influences on the sclerotial development of R. solani by dramatically suppressing their formation and maturation at 40.00 μg/ml, as well as their germination at 15.00 μg/ml. Morphological and ultrastructural observations showed that the hyphae of R. solani became twisted, shriveled, and deformed at the growing points after exposure to nematophin at 40.00 μg/ml, and that the subcellular fractions also became abnormal concurrently, especially the mitochondrial structure. These results indicate that nematophin has great potential to be used as a bio-pesticide in agricultural production.
研究领域:
农业科学
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微生物学
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植物病理学
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生物技术
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PLANT PATHOLOGY
·
2023
·
IF 1.8
Xiang Ligang,Yu Zhihe,Wang Hancheng,Cai Liuti,Hsiang Tom,Li Wenhong,Guo Moyan,Li Tong,Sun Meili
Tobacco, as one of the major cash crops in Guizhou province, China, is severely affected by diseases caused by Rhizoctonia solani . Azoxystrobin is a quinone outside inhibitor (QoI) fungicide extensively used to control various crop diseases in China. To evaluate the potential effect of azoxystrobin on the physiology, morphology, and sclerotial formation of Rhizoctonia solani , 104 isolates of R. solani from tobacco collected in Guizhou were evaluated for in vitro sensitivity to azoxystrobin, and scanning electron microscopy was used to observe the effects of azoxystrobin on mycelial morphology. The effects of azoxystrobin on reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), and succinate dehydrogenase (SDH) in hyphae at three time points (at 5, 10 and 15 days) and on sclerotial differentiation were examined. The effective concentration that caused 50% growth inhibition (EC 50 ) ranged from 0.3 to 552 μg/mL with a geometric mean of 8.4 μg/mL, and the frequency distribution of EC 50 values was unimodal. Scanning electron microscopy revealed that azoxystrobin caused folding, collapse and crumpling of hyphae. Under azoxystrobin stress, sclerotial differentiation was inhibited, and the production of hydrogen peroxide (H 2 O 2 ) and superoxide anion (O 2− ) as well as activities of SOD, CAT and SDH in hyphae increased significantly compared to the untreated control at day 5; while at day 10 or 15, H 2 O 2 production decreased significantly, CAT activity increased significantly, and O 2− , SOD, and SDH concentrations returned to normal levels. In general, the results provide a better understanding of the physiological effects of azoxystrobin on R. solani .
研究领域:
植物病理学
·
真菌生物学
·
农业科学
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产品关联
DOI
高影响因子
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