JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2015
·Vol. 39
·期 6
·2455-2465
·
IF 1.159
Kai Fan,Libing Chen,Xiaohong Wei,Jie He,Zankai Yan,Fengwei Yan
The antioxidative properties of rapeseed meal extract (RME; 0.014%, 0.028%, 0.042%, 0.056% and 0.070% w/w) were evaluated in soybean oil. Soybean oil oxidation was tested by measuring peroxide value, thiobarbituric acid reactive substances (TBARS) value, carbonyl value, acid value, conjugated diene value, p-anisidine value, iodine value and viscosity during accelerated storage at 68C for 19 days as compared with butylated hydroxyanisole (BHA) 0.014% (w/w). Results from different parameters were in agreement with each other; soybean oil containing RME 0.014% was better than soybean oil with BHA and control samples, with the exception of TBARS, which suggested higher efficiency of RME 0.028% over BHA. Antioxidative influence of soybean oil was not increased with increasing concentration of RME during 19 days of storage. Results revealed that RME added to soybean oil can retard the process of lipid peroxidation during storage. Practical Applications Oils are important functional components of foods and have a significant effect on the quality of foods. They not only contribute to flavor, odor, color and texture, but also confer a feeling of satiety and palatability to foods. However, the major problem in these oils lies in lipid oxidation during storage or food processing. The objective of this study was to evaluate the potentiality of rapeseed meal extract (RME) used as antioxidant. The antioxidative effects of RME was investigated in soybean oil under accelerated conditions and compared with butylated hydroxyanisole (BHA) during storage.
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2020
·
IF 4.939
Baofang Zhang,Renlian Chen,Zefan Yang,Yingxin Chen,Lihua Zhou,Yong Yuan
The heteroatom-doped porous carbon material as an alternative to commercial Pt/C catalysts in oxygen reduction reaction has attracted extensive attention. In this study, the rapeseed meal-based material (ARM-900) prepared by carbonization with high temperature and activation with ZnCl 2 had a porous structure and was doped with N and S heteroatoms. Compared to commercial Pt/C catalysts (onset potential of 0.95 V vs. RHE and limiting diffusion current of −5.7 mA cm −2 ), ARM-900 demonstrated excellent electrocatalytic performance with an onset potential of 0.98 V vs. RHE and limiting diffusion current of −8.1 mA cm −2 in O 2 saturated 0.1 M KOH solution. Meanwhile, ARM-900 had higher durability and more superior methanol tolerance than Pt/C catalyst. The excellent ORR performance of ARM-900 was derived from the formation of abundant pore structure and the doping of the autochthonous N and S heteroatoms. MFCs with ARM-900 as the cathode had the maximum power density of 808 mW/m 2 , which was obviously better than Pt/C (709 mW/m 2 ). This study provided an environment-friendly and effective strategy for the reuse of rapeseed meal and the preparation of N and S-doped non-metallic ORR catalysts.
研究领域:
能源材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2016
·Vol. 11
·期 6
·e0158351
·
IF 3.057
Yanzhou Zhang,Xunhang Li,Zhikui Hao,Ruchun Xi,Yujie Cai,Xiangru Liao
For the more efficient detoxification of phenolic compounds, a promising avenue would be to develop a multi-enzyme biocatalyst comprising peroxidase, laccase and other oxidases. However, the development of this multi-enzyme biocatalyst is limited by the vulnerability of fungal laccases and peroxidases to hydrogen peroxide (H 2 O 2 )-induced inactivation. Therefore, H 2 O 2 -resistant peroxidase and laccase should be exploited. In this study, H 2 O 2 -stable CotA and YjqC were isolated from the outer coat of Bacillus altitudinis SYBC hb4 spores. In addition to the thermal and alkali stability of catalytic activity, CotA also exhibited a much higher H 2 O 2 tolerance than fungal laccases from Trametes versicolor and Trametes trogii . YjqC is a sporulation-related manganese (Mn) catalase with striking peroxidase activity for sinapic acid (SA) and sinapine (SNP). In contrast to the typical heme-containing peroxidases, the peroxidase activity of YjqC was also highly resistant to inhibition by H 2 O 2 and heat. CotA could also catalyze the oxidation of SA and SNP. CotA had a much higher affinity for SA than B . subtilis CotA. CotA and YjqC rendered from B . altitudinis spores had promising laccase and peroxidase activities for SA and SNP. Specifically, the B . altitudinis spores could be regarded as a multi-enzyme biocatalyst composed of CotA and YjqC. The B . altitudinis spores were efficient for catalyzing the degradation of SA and SNP in rapeseed meal. Moreover, efficiency of the spore-catalyzed degradation of SA and SNP was greatly improved by the presence of 15 mM H 2 O 2 . This effect was largely attributed to synergistic biocatalysis of the H 2 O 2 -resistant CotA and YjqC toward SA and SNP.
研究领域:
微生物学
·
酶工程
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
ANIMAL FEED SCIENCE AND TECHNOLOGY
·
2021
·
IF 3.247
Xiaofeng Zhu,Lvyang Wang,Zhen Zhang,Liren Ding,Suqin Hang
As the increased rapeseed meal (RSM) production in last decades, improving the utilization of RSM is considered a major target in agricultural biotechnology. A series of in vitro studies were conducted to determine the effects of fiber-degrading enzymes and lactobacilli fermentation on the nutritional value of RSM. In experiment 1, the mixture of three strains of lactobacillus (L. reuteri L45, L. plantarum L47, and L. johnsonii L63) were applied to ferment RSM for 48 h after fiber-degrading enzymatic hydrolysis (a mixture of 10 FPU/g cellulase and 10 U/g pectinase) for 28 h. Four treatment groups were assigned: i) RSM without lactobacilli and enzymes (CON); ii) RSM with lactobacilli (LA); iii) RSM with enzymes (EN); and iv) RSM with enzymes and lactobacilli (EN + LA). Each group had 4 replicates. Lactobacilli growth characteristics were detected after the experiment. All RSM samples were dried and used for experiment 2. In experiment 2, samples of 4 groups were digested by pepsin and pancreatin to simulated small intestine digestion. Digestibility of dry matter and crude protein (CP) in vitro was measured. In experiment 3, undigested residues from each group were incubated with fresh pig rectum digesta as inoculums for 48 h to simulated the porcine large intestine fermentation in vitro . Accumulative gas production was recorded and modeled to estimate kinetics of gas production. Short-chain fatty acid (SCFA) concentration was measured at the end of fermentation. In experiment 1, the results demonstrated that reducing sugar concentration in EN was higher than that in CON. Compared with LA, lactic acid concentration and count of viable Lactobacillus were higher, and pH was lower in EN + LA. Rapeseed meal treated with EN + LA presented the lowest value of glucosinolate and the highest value of CP as well. In experiment 2, in vitro dry matter and CP digestibility of RSM was increased by 23 % and 20 %, respectively, by the addition of EN regardless of LA addition through a simulated small intestine digestion in vitro . The findings in experiment 3 showed that EN + LA reduced the accumulative gas production and SCFA compared with CON, indicating an improvement in utilization of carbohydrates and protein in both enzymatic hydrolysis and lactobacilli fermentation and in vitro digestion. Overall, fiber-degrading enzymes promoted the growth of lactobacilli on RSM, and the combination of fiber-degrading enzymatic hydrolysis and lactobacilli fermentation enhanced nutritional value and utilization of fiber and protein in RSM.
研究领域:
农业生物技术
·
动物营养
·
食品科学
✓ 已验证
DOI
高影响因子
mLife
·
2023
·Vol. 2
·期 2
·190-200
·
IF 0
Hongzhe Li,Jiazhi Ding,Longji Zhu,Fei Xu,Wenjing Li,Yanpo Yao,Li Cui
Application of agricultural waste such as rapeseed meal (RM) is regarded as a sustainable way to improve soil phosphorus (P) availability by direct nutrient supply and stimulation of native phosphate-solubilizing microorganisms (PSMs) in soils. However, exploration of the in situ microbial P solubilizing function in soils remains a challenge. Here, by applying both phenotype-based single-cell Raman with D 2 O labeling (Raman-D 2 O) and genotype-based high-throughput chips targeting carbon, nitrogen and P (CNP) functional genes, the effect of RM application on microbial P solubilization in three typical farmland soils was investigated. The abundances of PSMs increased in two alkaline soils after RM application identified by single-cell Raman D 2 O. RM application reduced the diversity of bacterial communities and increased the abundance of a few bacteria with reported P solubilization function. Genotypic analysis indicated that RM addition generally increased the relative abundance of CNP functional genes. A correlation analysis of the abundance of active PSMs with the abundance of soil microbes or functional genes was carried out to decipher the linkage between the phenotype and genotype of PSMs. Myxococcota and C degradation genes were found to potentially contribute to the enhanced microbial P release following RM application. This work provides important new insights into the in situ function of soil PSMs. It will lead to better harnessing of agricultural waste to mobilize soil legacy P and mitigate the P crisis. Impact statement Stimulation of native soil microorganisms to release soil P stocks is a sustainable means of agricultural production, but faces a major challenge in terms of the deciphering of in situ microbial phosphorus (P) solubilization functions. In this study, single-cell Raman spectroscopy and carbon, nitrogen and P (CNP) functional gene chips were used to reveal the phenotypes and genotypes of microbial P solubilization function in three farmland soils amended with agricultural waste of rapeseed meal (RM). Effects of RM on soil properties, active phosphate-solubilizing bacteria, and CNP functional genes that may contribute to P solubilization were revealed, providing important insights into the use of agricultural waste to improve P utilization efficiency.
研究领域:
微生物学
·
环境科学
·
农学
✓ 已验证
DOI
FOOD CHEMISTRY
·
2022
·
IF 9.231
Chong Xie,Wanmei Li,Rongguang Gao,Lihua Yan,Pei Wang,Zhenxin Gu,Runqiang Yang
The utilization of rapeseed meal in food is limited due to its abundant glucosinolates (GLs). In this study, an LC-MS/MS method for GLs determination in rapeseed meal was developed. Then, the degradation of GLs using rapeseed sprouts derived myrosinase (MYR) was investigated. Results showed that 11 kinds of GLs were identified in rapeseed meal. The LC-MS/MS method had a high linearity (R 2 greater than 0.9999), repeatability (RSD < 5%) and recovery rate (92%-102%). The optimum condition for hydrolyzing GLs in rapeseed meal was reacting for 4 h with the addition of 2236.35 U/g MYR, 9.63 μg/g ascorbic acid and 26.68 μg/g EDTA. Under this condition, more than 80% of GLs were degraded and the yields of isothiocyanates and oxazolidinone-2-thione were 859.30 μg/g and 685.59 μg/g, respectively. To conclude, this study reported a reliable method for GLs determination and an effective way to degrade GLs in rapeseed meal.
研究领域:
食品科学
·
分析化学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Miaochuan Lan,Yu Fu,Hongjie Dai,Liang Ma,Yong Yu,Hankun Zhu,Hongxia Wang,Yuhao Zhang
Rapeseed meal rich in protein serves as one of the major agricultural processing by-products; however, it is currently used as a fertilizer or feed, which causes a great waste of protein resources. The aim of this study was to enzymatically prepare rapeseed peptides (RPs) that can be self-assembled and used as a nanocarrier for encapsulation of hydrophobic β-carotene. During this process, the effects of rapeseed protein hydrolysis time, encapsulation time, and the ratio of β-carotene to RPs on the encapsulation results were investigated. The encapsulation efficiency (EE) and drug loading amount (LA) were used as the evaluation indicators. Through optimization of conditions, the optimal parameters of encapsulation were obtained, namely enzymatic hydrolysis for 60 min, encapsulation time for 3 h, the optimal ratio of β-carotene to peptide for 3/5, the EE for >80%, and the LA for 0.55 mg/mg. In addition, the encapsulation structure of RPs and β-carotene was systematically characterized by FTIR , TEM and CLSM, suggesting that β-carotene was successfully encapsulated by RPs. The present study contributes to a deep understanding of encapsulation of β-carotene by self-assembly of RPs.
研究领域:
食品科学
·
纳米技术
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2015
·
IF 2.416
Cunshan Zhou,Xiaojie Yu,Xiaopei Qin,Haile Ma,Abu ElGasim A. Yagoub,Jiali Hu
Kinetics modelling, DPPH radical scavenging activity and reducing power of rapeseed meal protein were investigated under simulated duodenum digestion. Kinetics of the reaction was considered in relation to initial substrate concentration, initial enzyme concentration, and hydrolysis time. Optimum hydrolysis conditions were trypsin concentration of 10 g/L, substrate concentration of 4 g/L, time of 40 min and temperature of 37 °C. From the results a general kinetic enzymolysis equation was suggested, providing a rational theoretical basis for determining the parameters of the reaction. The highest antioxidant activity was in accordance with the optimum reaction conditions. Antioxidant free amino acids increased after hydrolysis. Antioxidant oligopeptides of molecular weights of 2608, 1695 and 211 Da were identified. At a concentration of 2 mg/mL, the peptide fraction of CS-F 2 -CC 2 ; with molecular weight of 1000–1500 Da; showed the highest DPPH radical scavenging activity and reducing power, accounting for 70.32% and 0.538, respectively. The trypsin-hydrolysate displayed a potential capacity to scavenge free radicals and binding irons after duodenal digestion, appearing as promising ingredient to formulate functional foods with antioxidant activity.
研究领域:
食品科学
·
生物化学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 37
·15983-15993
·
IF 3.288
Yang Chenxi,Li Juan,Wang Jian,Guo Zhen,Wang Huanyuan,Shi Chendi,Hua Dongwen,Li Ling
In this work, we report the novel fabrication of polymerized octadecylsiloxane (PODS)/4-aminoazobenzene (AAB) coated rapeseed meal (RSM) using a facile one-pot dip-coating method. The as-prepared RSM-PODS/AAB employs RSM as a carrier, the PODS coating endows the material with hydrophobicity, thus improving the loading rate of pinoxaden (Pxd) of RSM-PODS/AAB, and the AAB moieties act as light-driven “stirrers” to stir the release of Pxd. The structure and composition were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, ultraviolet-visible spectroscopy and contact angle measurements. The experimental results indicate that the loading rate of Pxd was increased by 21.38% compared with pristine RSM. More importantly, the Pxd-loaded RSM-PODS/AAB showed perfect UV-visible and sunlight controlled release performance, the excellent biodegradability of RSM effectively supplying the nutrient elements of plant growth in the soil. Overall, this system provides a promising way to improve controlled drug release with UV-visible- and sunlight-responsive behavior.
研究领域:
材料科学
·
农业化学
·
环境科学
·
药学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）