AGRICULTURAL RESEARCH
·
2024
·Vol. 13
·期 2
·198-203
·
IF 1.4
Lv Na,Li Li,Wang Na,Guo Chang-jie,Zhang Hao-yan
Snap bean ( Phaseolus vulgaris L.) is very sensitive to low temperature during postharvest storage. Methyl jasmonate (MeJA) is a volatile hormone ubiquitous in many kinds of plants and plays important role in regulating plant physiological functions. The alleviating effect of MeJA treatment on chilling injury (CI) in postharvest snap bean was studied. We found MeJA treatment markedly decreased the index of chilling injury, electrolyte leakage and content of malondialdehyde. MeJA-treated snap bean exhibited higher levels of total soluble solids and titratable acidity. Similarly, MeJA could prevent the degradation of chlorophyll and ascorbic acid. Not only total antioxidant capacity but also metal chelating ability of MeJA-treated snap bean was stronger than untreated control. MeJA-treated snap bean showed higher scavenging activities on superoxide radical (O 2 .- ), hydroxyl radical (·OH) and 1,1-diphenyl-2-trinitrophenylhydrazine radical either. MeJA application stimulated the activities of ascorbate peroxidase (APX), peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) in snap bean. In conclusion, this study reveals MeJA can be used to relieve chilling injury and maintain the quality of snap bean during low-temperature storage. 8 µM MeJA is the optimal concentration to alleviate chilling injury of snap bean.
研究领域:
植物学
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植物生理学
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采后生物学
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食品科学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Qi Han,Youwen Xing,Lulu Yan,Lulu Cao,Zehao Li,Jiandong Jiang,Xihui Xu,Chen Chen
Cyromazine, a triazine insecticide, raises food safety concerns due to residues in vegetables like cowpeas. Microbial metabolism is key for pesticide elimination, but bacteria efficient in cyromazine degradation are limited, with uncharacterized enzymes. This study isolated a highly efficient cyromazine-degrading bacterium, Mycobacterium sp. M15, from a cowpea field. M15 utilized cyromazine as the sole carbon source for its growth and completely degraded 0.5 mM cyromazine within 24 h. The degradation pathway involved hydrolyzing cyromazine to N-cyclopropylammeline and further to N-cyclopropylammelide, with amino groups removed sequentially. The cyclopropylamine group in N-cyclopropionamide continued to hydrolyze to cyanuric acid. A protein, CriA, identified as an aminohydrolase in M15, degraded cyromazine to N-cyclopropylammeline. Using CriA reduced cyromazine residues on cowpea surfaces and completely degraded them in immersion solutions. These findings offer insights into cyromazine’s microbial degradation mechanism and highlight the potential of cyromazine-degrading enzymes in enhancing food safety.
研究领域:
环境科学
·
微生物学
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生物技术
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农药化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 8
·4514
·
IF 4.9
Chaopu Ding,Yunfei Zhang,Chongbin Chen,Junfang Wang,Mingda Qin,Yu Gu,Shujing Zhang,Lanying Wang,Yanping Luo
In agriculture, soil-borne fungal pathogens, especiallyFusarium oxysporumstrains, are posing a serious threat to efforts to achieve global food security. In the search for safer agrochemicals, silica nanoparticles (SiO2NPs) have recently been proposed as a new tool to alleviate pathogen damage including Fusarium wilt. Hollow mesoporous silica nanoparticles (HMSNs), a unique class of SiO2NPs, have been widely accepted as desirable carriers for pesticides. However, their roles in enhancing disease resistance in plants and the specific mechanism remain unknown. In this study, three sizes of HMSNs (19, 96, and 406 nm as HMSNs-19, HMSNs-96, and HMSNs-406, respectively) were synthesized and characterized to determine their effects on seed germination, seedling growth, andFusarium oxysporumf. sp.phaseoli(FOP) suppression. The three HMSNs exhibited no side effects on cowpea seed germination and seedling growth at concentrations ranging from 100 to 1500 mg/L. The inhibitory effects of the three HMSNs on FOP mycelial growth were very weak, showing inhibition ratios of less than 20% even at 2000 mg/L. Foliar application of HMSNs, however, was demonstrated to reduce the FOP severity in cowpea roots in a size- and concentration-dependent manner. The three HMSNs at a low concentration of 100 mg/L, as well as HMSNs-19 at a high concentration of 1000 mg/L, were observed to have little effect on alleviating the disease incidence. HMSNs-406 were most effective at a concentration of 1000 mg/L, showing an up to 40.00% decline in the disease severity with significant growth-promoting effects on cowpea plants. Moreover, foliar application of HMSNs-406 (1000 mg/L) increased the salicylic acid (SA) content in cowpea roots by 4.3-fold, as well as the expression levels of SA marker genes ofPR-1(by 1.97-fold) andPR-5(by 9.38-fold), and its receptor gene ofNPR-1(by 1.62-fold), as compared with the FOP infected control plants. Meanwhile, another resistance-related gene ofPALwas also upregulated by 8.54-fold. Three defense-responsive enzymes of POD, PAL, and PPO were also involved in the HMSNs-enhanced disease resistance in cowpea roots, with varying degrees of reduction in activity. These results provide substantial evidence that HMSNs exert their Fusarium wilt suppression in cowpea plants by activating SA-dependent SAR (systemic acquired resistance) responses rather than directly suppressing FOP growth. Overall, for the first time, our results indicate a new role of HMSNs as a potent resistance inducer to serve as a low-cost, highly efficient, safe and sustainable alternative for plant disease protection.
研究领域:
农业
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植物病理学
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纳米技术
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分子生物学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Dan Liu,Wei Tang,Yue Xin,Ze-Xia Wang,Xiao-Jun Huang,Jie-Lun Hu,Jun-Yi Yin,Shao-Ping Nie,Ming-Yong Xie
Polysaccharides were extracted by hot water and alkali in sequence from Dolichos lablab L. hull, and further purified by ion-exchange and gel columns. Hot water extracted D. lablab hull polysaccharide (DLHP) was rich in glucuronoxylan and pectin, and alkali extracted polysaccharide (DLHAP) mostly embraced glucuronoxylan. The structures of purified glucuronoxylans from DLHP and DLHAP were mainly analyzed by HPAEC-PAD, methylation combined with GC–MS, NMR and SEC-MALLS. DLHP-1 was identified as acetylated glucuronoxylan containing →4)-β-Xyl p- (1→ backbone with substitution at O -2 site by α-Glc p A/4- O -methyl-α-Glc p A. The molar ratio of β-Xyl p to α-Glc p A was 6.9:1, and acetylation was mainly at O -3 site of β-Xyl p with acetylation degree of 21.5%. DLHP-1 and DLHP-2 had similar physicochemical properties, except for molecular weight ( M w ). DLHAP-1 was the non-methylated glucuronoxylan almost without acetylation, and it had the molar ratio of β-Xyl p to α-Glc p A of 5.6:1. Besides, DLHP-1 ( M w of 20.0 × 10 3 g mol −1 ) adopted semi-flexible chain, while DLHAP-1 ( M w of 15.4 × 10 3 g mol −1 ) showed flexible chain. These results provided a structural basis for study on polysaccharides from D. lablab hull, which was benefit for understanding biological activities and developing functional food or pharmaceuticals of D. lablab .
研究领域:
植物化学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Juyi Song,Chang Liu,Xin Zhang,Hui Chen
In this study, an active film was developed based on β-glucan (BG)/polyvinyl alcohol (PVA)/clove essential oil (CEO) and its preservation effects on fresh broad bean pods were examined. The impact of different BG:PVA ratios (50:50, 40:60 and 30:70) and CEO concentrations (0.25%, 0.50% and 1.00%) on the capacities of film were investigated. A BG:PVA ratio of 40:60 and CEO content of 0.5% (w/w) were adopted following the optimization of the physical, mechanical and barrier capacities of the blended film. Subsequent characterization of the film via Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy suggested that the BG hydroxyl groups interacted well with the PVA and CEO, and the good heat stability of the BG/PVA-CEO film was confirmed via thermal gravimetric analysis-differential scanning calorimetry. Importantly, the BG/PVA-CEO film also showed good antimicrobial activity. Moreover, the BG/PVA-0.5% CEO film effectively preserved fresh broad bean pods over a 30-day storage period, based on assessments of appearance, firmness, weight loss and the enzyme activities. These results indicate the promising applicability of the novel BG/PVA-CEO films as active coating materials for the preservation of fresh produce and food products.
研究领域:
食品科学
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材料科学
·
农业工程
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 11
·2160
·
IF 5.2
Si Li,Fangwei Liu,Mulan Wu,Yuhao Li,Xiaoxiao Song,Junyi Yin
In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans. The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared. The results indicated significant differences (p< 0.05) in nutritional composition, such as protein and soluble sugar content. Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters. In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying. The chemometric analysis revealed that the bioactive compounds in broad beans dried by three different methods were primarily composed of flavonoids, organic acids, and amino acids with significant differentiation. Notably, freeze-dried and sun-dried broad beans exhibited a higher concentration of differential substances.Keywords:broad beans;drying method;nutrition composition;bioactive substances
研究领域:
食品科学
·
营养学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT CELL
·
2024
·
IF 10
Wu Xinyang,Chen Shuting,Zhang Zixin,Zhou Weixin,Sun Ting,Ning Kang,Xu Min,Ke Xubo,Xu Pei
Virus-induced drought tolerance presents a fascinating facet of biotic-abiotic interaction in plants, yet its molecular intricacies remain unclear. Our study shows that cowpea mild mottle virus (CPMMV) infection enhances drought tolerance in common bean (Phaseolus vulgaris) plants through a virus-derived small interfering RNA (vsiRNA)-activated autophagy pathway. Specifically, a 21-bp vsiRNA originating from the CPMMV Triple Gene Block1 (TGB1) gene targeted the 5' untranslated region (UTR) of the host Teosinte branched 1, Cycloidea, Proliferating Cell Factor (TCP) transcription factor gene PvTCP2, independent of the known role of TGB1 as an RNA silencing suppressor. This targeting attenuated the expression of PvTCP2, which encodes a transcriptional repressor, and in turn upregulated the core autophagy-related gene (ATG) PvATG8c, leading to activated autophagy activity surpassing the level induced by drought or CPMMV infection alone. The downstream EARLY RESPONSIVE TO DEHYDRATION (ERD) effector PvERD15 is a homologue of Arabidopsis thaliana AtERD15, which positively regulates stomatal aperture. PvERD15 was degraded in PvATG8c-mediated autophagy. Therefore, we establish a TGB1-PvTCP2-PvATG8c-PvERD15 module as a trans-kingdom fine-tuning mechanism that contributes to virus-induced drought tolerance in plant-drought-virus interactions.
研究领域:
植物病毒学
·
分子生物学
·
植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Yiyun Gao,Wenjun Xiao,Chenhua Gu,Sheng Yu,Shengke Li,Aihua Zou
Background Conventional pesticide formulations are often griped about inefficiency due to the low biological uptake after spraying. Controlled release nanopesticides can precisely release pesticides in response to specific stimuli, thereby killing pests and pathogens with the least effective concentration. This study aims to develop nanocapsules-based photo decomposable nanopesticides for efficient pesticide control. Results The target nanopesticides were successfully fabricated by using layer-by-layer assembly of azobenzene grafted hyaluronic acid (Azo-HA) and PolyDADMAC, confirmed by UV–vis, DLS, Zeta potential and TEM measurements. Particle size and Zeta potential of the fabricated nanocapsules were 220 nm and + 46.1 mV, respectively, and the nanocapsules were found to maintain stable for up to 30 days. The optimized drug loading and encapsulation ratio of Imidacloprid (IMI) in IMI/Azo-HA@PolyDADMAC were 21.5% and 91.3%, respectively. Cumulative release of IMI from the nanopesticides was increased from ~50% to ~95% upon UV-light irradiation (365 nm). LC 50 value of the nanopesticides towards Aphis craccivora Koch was decreased from 2.22 mg/L to 0.55 mg/L upon UV-light irradiation. Conclusion The trans to cis transformation of Azo group in HA will decompose IMI/Azo-HA@PolyDADMAC nanopesticides upon UV irradiation, thus facilitated the IMI release, resulting in decreased concentration of pesticides for efficient pesticide control. Our work demonstrated the great potential of light-responsive nanocapsules as controlled release nanocarrier for efficient and eco-friendly pesticide-control in sustainable agriculture. This article is protected by copyright. All rights reserved.
研究领域:
材料科学
·
纳米技术
·
农药科学
·
农业工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2016
·Vol. 8
·期 43
·18400-18411
·
IF 7.76
Sen Lin,Tong Li,Peiling Xie,Qing Li,Bailiang Wang,Lei Wang,Lingli Li,Yuqin Wang,Hao Chen,Kaihui Nan
Targeted delivery of cytotoxic drugs to tumour tissue has great importance for successful chemotherapy. Legumain is an asparaginyl endopeptidase that is highly up-regulated in a number of solid tumours. The aim of this work was to prepare a novel hyaluronic acid (HA) based legumain sensitive nanogel for the delivery of doxorubicin with a high targeting efficiency both in vitro and in vivo. The legumain sensitive property is achieved by the conjugation of doxorubicin with HA via a legumain substrate peptide bridge. This HA derivative is further crosslinked in a water/oil solvent system to form a polygonal nanogel. Doxorubicin released in the tumour tissue is sustained thanks to the combined action of legumain and hyaluronidase, which are both overexpressed in tumour tissues. Hyaluronic acid could act as a targeting agent to CD44 (HA receptor), which further improved the in vivo target effect and enhanced in vitro cellular uptake. The developed nanogel exhibited a high therapeutic index that improved tumour inhibition effects and reduced system toxicity in a lung cancer mice model. These results highlighted the advantages of using this multi-functional material for a successful delivery of doxorubicin against cancer.
研究领域:
药学
·
癌症研究
·
纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2022
·
IF 6.819
Shanshan Zheng,Yue Cai,Yulu Hong,Yubei Gong,Licheng Gao,Qingyong Li,Le Li,Xuanrong Sun
After molecule targeted drug, monoclonal antibody and antibody–drug conjugates (ADCs), peptide–drug conjugates (PDCs) have become the next generation targeted anti-tumor drugs due to its properties of low molecule weight, efficient cell penetration, low immunogenicity, good pharmacokinetic and large-scale synthesis by solid phase synthesis. Herein, we present a lytic peptide PTP7-drug paclitaxel conjugate assembling nanoparticles (named PPP) that can sequentially respond to dual stimuli in the tumor microenvironment, which was designed for passive tumor-targeted delivery and on-demand release of a tumor lytic peptide (PTP-7) as well as a chemotherapeutic agent of paclitaxel (PTX). To achieve this, tumor lytic peptide PTP-7 was connected with polyethylene glycol by a peptide substrate of legumain to serve as hydrophobic segments of nanoparticles to protect the peptide from enzymatic degradation. After that, PTX was connected to the amino group of the polypeptide side chain through an acid-responsive chemical bond (2-propionic-3-methylmaleic anhydride, CDM). Therefore, the nanoparticle (PPP) collapsed when it encountered the weakly acidic tumor microenvironment where PTX molecules fell off, and further triggered the cleavage of the peptide substrate by legumain that is highly expressed in tumor stroma and tumor cell surface. Moreover, PPP presents improved stability, improved drug solubility, prolonged blood circulation and significant inhibition ability on tumor growth, which gives a reasonable strategy to accurately deliver small molecule drugs and active peptides simultaneously to tumor sites.
研究领域:
药学
·
纳米技术
·
癌症研究
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Lina Fu,Qi Gu,Su Zhang,Jiandong Wang,Zihui Cai,Yujie Fu
Developing green approaches for the simultaneous extraction and encapsulation of bioactive compounds is crucial in the food fields. In the present study, a one-pot approach to extract and encapsulate polyphenols from Cajanus cajan ( C. cajan ) leaves via 2-Hydroxypropyl- β -cyclodextrin (2-HP- β -CD) was established. The extraction efficiency of 2-HP- β -CD for polyphenols in C. cajan leaves (32.75 mg/g) was higher than that of ethanol (23.42 mg/g). In the release study, the encapsulated polyphenols nanoparticles (PNPs) showed a complete release property (the release rate was 96%). These PNPs had strong activities of scavenging DPPH radicals (IC 50 = 0.52 mg/mL), ·OH radicals (IC 50 = 1.42 mg/mL), and O 2– radicals (IC 50 = 0.30 mg/mL), and had inhibitory effects on gram-positive bacteria ( B. subtilis , S. aureus ) and HepG2 cells (IC 50 = 279.6 μg/mL). Additionally, almost 70% of polyphenols in PNPs were still retained over 15 days in both the gummy candy and beverage. Overall, the proposed method can provide a scientific reference for the manufacture of food and healthful products.
研究领域:
食品科学
·
药理学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Tingting Liu,Jian Luo,Shangke Liu,Tongtong Li,Haolin Li,Lingyan Zhang,Wei Mu,Nan Zou
Neonicotinoid insecticides have been widely used due to their excellent systemic activity and high insecticidal activity, but the problems of low utilization rate and environmental risk have attracted widespread attention. Controlled-release technology is an approach to realize the efficient utilization of pesticides and reduce environmental pressure. In this study, clothianidin (CLO) controlled-release granules ( [email protected] TA (tannic acid)/Fe (III)) were prepared with TA/Fe (III) coordination chelate as the coating material. These granules exhibited the core-shell structure with 500–1200 µm of particle size, and had obvious release performance and hydrolysis behavior of coating materials. Pot experiments by root application showed that the [email protected] /Fe (III) showed balanced and lasting control efficacy to broad bean aphids. The plants have a stronger capacity for absorption and enrichment and a higher utilization rate of CLO for [email protected] /Fe (III), than those for 10% suspension concentrate (SC). One of the hydrolysates of coating materials, TA, a polyphenolic antioxidant, could improve the bioaccumulation amount and alleviating the oxidative stress response of CLO in plants. Our study illustrates that the controlled-release granules base on TA have efficient controlled-release properties and free radical scavenging performance that may eventually be used as pesticide carriers and antioxidants in the field of plant protection.
研究领域:
农业科学
·
农药科学
·
植物保护
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）