Animals
·
2023
·Vol. 13
·期 9
·1447
·
IF 3
Zhenglie Dai,Huixian Wang,Jinsong Liu,Haoran Zhang,Qing Li,Xiaorong Yu,Ruiqiang Zhang,Caimei Yang
Simple SummaryAntibiotics are banned in animal feed, and substances used to replace antibiotics are being found continuously.Yucca schidigera,Quillaja saponaria, andYucca saponinare natural plants and their components, which have many beneficial health functions for animals, are suitable as animal feed additives to replace antibiotics. By comparing the impacts of three substances on the growth, immunity, and intestinal health of broilers, this study found thatYucca schidigera,Quillaja saponaria, andYucca saponinall promote the healthy growth of broilers, andYucca saponinhas better effects than the other two substances.AbstractThe purpose of this study is to investigate the effects ofYucca saponin(YSa),Yucca schidigera(YS), andQuillaja Saponaria(QS) on growth performance, nitrogen metabolism, immune ability, antioxidant capability, and intestinal flora of yellow-feather broilers. This study randomly divided a total of 480 1-day yellow-feather broilers into 4 treatment groups. Factors in the 4 groups included CON group (basic diet), YSa group (basic diet mixed with 500 mg/kg YSa), YS group (basic diet mixed with 500 mg/kg YS), and QS group (basic diet mixed with 500 mg/kg QS). Throughout the 56-day study period, YSa, YS, and QS groups had higher average daily gain in broilers than the CON group (p< 0.01). The YS group had a lower feed gain ratio (F: G) in broilers than the CON group (p< 0.05). YSa, YS, and QS showed increased serum immunoglobin A (IgA), immunoglobin Y (IgY), immunoglobin M (IgM), and total antioxidant capacity (T-AOC) levels; enhanced acetic acid, butyric acid, and valeric acid levels of cecal content; and reduced contents of ammonia nitrogen, urea nitrogen, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and malondialdehyde (MDA) in serum in broilers (p< 0.05). The relative abundance of Lachnoclostridium in the QS group was decreased compared with that in the CON group (p< 0.05). Higher IgA and IgY sera contents were observed in the YS group compared to the YSa and QS groups (p< 0.05). In contrast with the QS group, the serum IL-6 concentration of the YS group was reduced (p< 0.05). In conclusion, YSa, YS, and QS promoted growth performance, nitrogen metabolism, immunity, antioxidant capability, and intestinal flora in broilers. Through the comparison of YSa, YS, and QS, it was found that YS is more suitable as a feed additive to ameliorate the healthy growth of broilers.Keywords:Yucca saponin;Quillaja saponaria;yellow-feather broilers;growth performance;intestinal flora
研究领域:
动物营养
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家禽科学
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饲料添加剂
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免疫学
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抗氧化研究
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微生物组研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Yu-Min Zhang,Qi Luo,Meng Lu,Xue Gong,Ya-Wei Guo,Xiang-Bin Zeng,Ying Zhu,Dan Shu,Yue-Ling Lin,Xu-Ran Guo,Zhang-Yin Ming
Ethnopharmacological relevance Ilex pubescens Hook. & Arn. is a traditional Chinese medicine for promoting blood circulation. Ilexsaponin A1 (IsA), a monomer of the compound, exhibits pro-angiogenic, anti-apoptotic and anti-inflammatory activities. Nevertheless, the pharmacological effects and specific mechanisms by which IsA affects platelets remain unknown. Aim of the study This study aims to investigate the antiplatelet effects of IsA and the underlying molecular mechanisms. Materials and methods Platelet aggregation and ATP release were assessed using platelet aggregometry. Flow cytometry was employed to evaluate the exposure of P-selectin, integrin α Ⅱb β 3 activation and calcium mobilization. Fluorescence microscopy was applied to observe platelet spreading. Clot retraction was imaged by digital camera. Protein phosphorylation regulation of major signaling pathways in platelets was determined by immunoblotting analysis. Doppler flowmetry was used to investigate the in vivo effect of IsA on FeCl 3 -induced carotid artery injury model. Tail vein transection was used to measure bleeding time. Results IsA dose-dependently inhibited platelet aggregation and ATP release induced by collagen, U46619, thrombin and ADP. It also suppressed thrombin-induced P-selectin exposure and PAC-1 binding. Furthermore, IsA inhibited intracellular Ca 2+ mobilization and the inward flow of extracellular Ca 2+ . It also influenced integrin α Ⅱb β 3 outside-in signaling pathways, including the inhibition of platelet spreading, clot retraction and phosphorylation of outside-in signaling molecules. In addition, IsA suppressed the phosphorylation of Syk-PLCγ2, PI3K-Akt-GSK3β and MAPKs proteins, which are downstream effectors of the collagen and thrombin receptors. Conclusion IsA inhibited platelet function and thrombus formation. This has potential to be developed into a novel therapeutic agent for the treatment of thrombotic diseases.
研究领域:
药理学
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分子生物学
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中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yongxian Niu,Xi zhang,Wenbing Yu,Han Yin,Rui Lu,Zhenggang Liu,Fang Lu
Herein, a renewable system consisted of tartaric acid and isopropanol was developed to produce diosgenin efficiently from Dioscorea zingiberensis rhizomes. The yield of diosgenin reached 4.15% comparable to the highest value reported in previous researches. The combination of experiment and DFT calculation revealed that the acid-alcohol system could regulate the hydrogen bond interaction of each component in the rhizomes to disassemble the connection network and promote the release of saponin. Meanwhile, the intramolecular hydrogen bond of tartaric acid and the steric hindrance effect of isopropanol could prevent the esterification reaction and maintain the strong acidity of the system for the high yield of diosgenin. The scale-up experiment with a 100-fold feeding capacity was also conducted successfully. Furthermore, the technological process integrated with purification of diosgenin, recovery of organic acid and solvent and upgrading of residual solid to porous carbon materials has been established systematically to verify the prospective of industrialization.
研究领域:
植物化学
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药物化学
✓ 已验证
产品关联
DOI
高影响因子
CATALYSIS LETTERS
·
2022
·Vol. 152
·期 11
·3453-3464
·
IF 2.936
Zhao Mengqi,Pan Zhiquan,Chen Qingrong,Zhou Hong
Diosgenin is an important intermediate of drugs, the special structure similar to hormone determines its important position in drug synthesis. Diosgenin was generally obtained by sulfuric acid hydrolysis of dioscorea, which produced too much acid wastewater, and caused environmental issues. A new solid acid based on TiO 2 was designed and synthesized through the sulfonation of the prepared TiO 2 , the structure was fully characterized by FTIR, SEM, EDX, XPS and XRD. Under the optimized conditions, the yield of diosgenin reached 30.75%, the value was much higher than that of H 2 SO 4 of the same quality. To explore the factors that affect the yield, the usage amount of the solid acid, the temperature, the solvent volume and the extraction time were taken into the analysis, and the results of the recycling experiments indicated that the prepared solid acid can be used for three time and maintain its high activity. A highly efficient solid acid catalyst for diosgenin extraction from saponins was successfully synthesized, and the extraction method was environmentally friendly. Graphical Abstract
研究领域:
药物化学
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环境科学
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DOI
高影响因子
MOLECULES
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2020
·Vol. 25
·期 22
·5295
·
IF 3.267
Xinyu Zhao,Ruyi Chen,Yueyue Shi,Xiaoxi Zhang,Chongmei Tian,Daozong Xia
This study aimed to isolate, prepare and identify the main flavonoids from a standardizedSmilax glabraflavonoids extract (SGF) using preparative HPLC, MS,1H NMR and13C NMR, determine the contents of these flavonoids using UPLC, then compare their pharmacological activities in vitro. We obtained six flavonoids from SGF: astilbin (18.10%), neoastilbin (11.04%), isoastilbin (5.03%), neoisoastilbin (4.09%), engeletin (2.58%) and (−)-epicatechin (1.77%). The antioxidant activity of six flavonoids were evaluated by determining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS+) radical scavenging activity and ferric reducing antioxidant power (FRAP). In addition, the anti-inflammatory activity of six flavonoids were evaluated by determining the production of cytokines (IL-1β, IL-6), nitric oxide (NO) using enzyme linked immunosorbent assay and the NF-κB p65 expression using Western blotting in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that (−)-epicatechin, astilbin, neoastilbin, isoastilbin and neoisoastilbin had strong antioxidant activities, not only in DPPH and ABTS+radicals scavenging capacities, but in FRAP system. Furthermore, all the six flavonoids could significantly inhibit the secretion of IL-1β, IL-6, NO (p< 0.01) and the protein expression of NF-κB p-p65 (p< 0.01) in LPS-stimulated RAW264.7 cells. This study preliminarily verified the antioxidant and anti-inflammatory activities of six flavonoids inS. glabra.Keywords:flavonoids;Smilax glabra;preparative HPLC;antioxidant;anti-inflammatory
研究领域:
药理学
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生物化学
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植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Oxidative Medicine and Cellular Longevity
·
2016
·
IF 4.492
Zhuo Fan,Ting Wen,Yaoxu Chen,Lijie Huang,Wei Lin,Chunxia Yin,Wen Tan
channel is an important mediator or factor in physiological and pathological metabolic pathway. Activation of channel has been identified to be a critical step in the cardioprotective mechanism against IR injury. On the other hand, desensitization of the channel to its opener or the metabolic ligand ATP in pathological conditions, like cardiac hypertrophy, would decrease the adaption of myocardium to metabolic stress and is a disadvantage for drug therapy. Isosteviol, obtained by acid hydrolysis of stevioside, has been demonstrated to play a cardioprotective role against diseases of cardiovascular system, like anti-IR injury, antihypertension, antihyperglycemia, and so forth. The present study investigated the effect of isosteviol (STV) on sarc channel current induced by pinacidil and mitochondrial flavoprotein oxidation induced by diazoxide. Our results showed that preincubating cells with STV not only increased the current amplitude and activating rate of sarc channels induced by pinacidil but also potentiated diazoxide-elicited oxidation of flavoprotein in mitochondria.
研究领域:
药理学
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心血管生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL SCIENCE
·
2023
·Vol. 30
·期 1
·1-14
·
IF 11
Shen Weiyi,Shao Wentao,Wang Qihan,Wang Bo,Zhao Gang,Gu Aihua,Jiang Zhaoyan,Hu Hai
Background Cholesterol gallstone disease is a common disease. Reducing cholesterol burden is important to prevent/treat gallstone. In this study, we investigated the application of diosgenin (DG) to prevent the formation of gallstone in mice.Methods Adult male C57BL/6J mice were fed with the lithogenic diet (LD) only or LD supplemented with DG or ezetimibe for 8 weeks. Incidences of gallstone formation were documented. Intestine and liver tissues were collected to measure the lipid contents and expression of genes in cholesterol metabolism. Caco2 cells were treated with DG to monitor the regulation on cholesterol absorption and the transcriptional regulation of Npc1l1 gene. Changes of gut microbiota by DG was analyzed. Intraperitoneal injection of LPS on mice was performed to verify its effects on STAT3 activation and Npc1l1 expression in the small intestine.Results LD led to 100% formation of gallstones in mice. In comparison, dietary DG or ezetimibe supplementary completely prevents gallstones formation. DG inhibited intestinal cholesterol absorption in mice as well as in Caco2 cells by down-regulation of Npc1l1 expression. DG could directly inhibit phosphorylation of STAT3 and its transcriptional regulation of Npc1l1 expression. Furthermore, DG could modulate gut microbiota profiles and LPS mediated STAT3 activation and Npc1l1 expression.Conclusion Our results demonstrated that dietary DG could inhibit intestinal cholesterol absorption through decreasing NPC1L1 expression to prevent cholesterol gallstone formation.
研究领域:
药理学
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胃肠病学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Yunchang Fan,Wenjin Li,Sheli Zhang,Shuaihao Sun,Lei Yang
In Asian, rhizoma smilacis glabrae (RSG) is viewed as traditional food , and herb, and its main bioactive component is astilbin. The extraction of astilbin from RSG by traditional solvents suffers from the lower extraction efficiency, and longer extraction time. In this work, green solvents, vitamin B 3 -based protic ionic liquids (VB 3 PILs) were used to extract astilbin from RSG in combination with microwave-assisted extraction (MAE) method. Compared with the traditional solvents, for instance alcohols, and β -cyclodextrin ( β -CD) aqueous solution, the VB 3 PIL, nicotinamidium lactate (NALA), exhibits higher extraction ability, and shorter extraction time (20 s). The extraction ability of VB 3 PILs depends on their physicochemical properties, such as viscosity, hydrophobicity, and hydrogen-bonding ability. To comprehensively conclude the above results, it can be suggested that VB 3 PILs are green and, high efficient solvents for the extraction of astilbin from RSG, which may have great potential applications in extracting natural products from plant food sources.
研究领域:
天然产物化学
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绿色化学
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药用植物学
✓ 已验证
DOI
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
·
2017
·
IF 3.498
Zhuo Fan,Nanying Lv,Xiao Luo,Wen Tan
Cardiac hypertrophy is a thickening of the heart muscle that is associated with cardiovascular diseases such as hypertension and myocardial infarction. It occurs initially as an adaptive process against increased workloads and often leads to sudden arrhythmic deaths. Studies suggest that the lethal arrhythmia is attributed to hypertrophy-induced destabilization of cardiac electrical activity, especially the prolongation of the action potential. The reduced activity of I to is demonstrated to be responsible for the ionic mechanism of prolonged action potential duration and arrhythmogeneity. Isosteviol (STV), a derivative of stevioside, plays a protective role in a variety of stress-induced cardiac diseases. Here we report effects of STV on rat ISO-induced hypertrophic cardiomyocytes. STV alleviated ISO-induced hypertrophy of cardiomyocytes by decreasing cell area of hypertrophied cardiomyocytes. STV application prevented the prolongation of action potential which was prominent in hypertrophied cells. The decrease and increase of current densities for I to and I CaL observed in hypertrophied myocytes were both prevented by STV application. In addition, the results of qRT-PCR suggested that the changes of electrophysiological activity of I to and I CaL are correlated to the alterations of the mRNA transcription level.
研究领域:
药理学
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心血管病学
✓ 已验证
DOI
PubMed
高影响因子
Metabolites
·
2024
·Vol. 14
·期 1
·49
·
IF 3.4
Lili Yan,Yuchan Deng,Yulan Du,Xutong Fang,Xin Fang,Qiang Zhang
Smilax chinaL. (Chinaroot) is a natural herb that has multiple uses, such as being used to make tea and food. Both its roots and leaves have different uses due to their unique components. In this study, we analyzed the extract ofS. china.roots using LC-HRMS and evaluated the neuroprotective effects and metabolic regulation ofS. chinaonCaenorhabditis elegans.Chinaroot extract prolonged the life span of healthy nematodes, delayed the paralysis time of transgenic CL4176, and reduced the level of β-amyloid deposition in transgenic CL2006. The comprehensive analysis of metabolomics and qRT-PCR revealed that Chinaroot extract exerted neuroprotective effects through the valine, leucine and isoleucine degradation and fatty acid degradation pathways. Moreover, we first discovered that the expressions ofT09B4.8,ech-7, andagxt-1were linked to the neuroprotective effects of Chinaroot. The material exerted neuroprotective effects by modulating metabolic abnormalities in AD modelC. elegans. Our study provides a new foundation for the development of functional food properties and functions.Keywords:Alzheimer’s disease;Smilax chinaL.;β-amyloid;Caenorhabditis elegans;metabolomics;neuroprotective
研究领域:
神经科学
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药理学
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代谢组学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AUTOIMMUNITY
·
2020
·
IF 2.125
Cao Yongjun,Qiao Nan,Sun Yumeng,Jin Xiaowen,Wen Weibo
Dioscin has been used as a treatment for Hashimoto’s thyroiditis (HT) in China. However, the molecular mechanisms governing the modes of action of dioscin have not been elucidated. In this study, flow cytometry and Western blotting were used to identify the proportions of CD4+CD25+ regulatory T (Treg) cells and the expression of forkhead box P3 (Foxp3) and SUMO-specific protease 1 (SENP1) in HT patients’ peripheral blood mononuclear cells (PBMCs). A pTg-induced rat model of HT was established by injection of 100 μg pTg. Then, the model rats were randomly divided into three groups (n = 5): control (NC), model (HT) and dioscin treatment. After oral administration of dioscin each day for two weeks, CD4+CD25+Foxp3+ Treg cells were analysed by flow cytometry, and the protein expression levels of SENP1, Foxp3, SUMO-1 and SUMO-2/3 were measured by Western blotting. Co-immunoprecipitation (Co-IP) was used to identify the SUMOylation of interferon regulatory factor 4 (IRF4). The results showed that the proportions of CD4+CD25+ Treg cells and the expression of Foxp3 were significantly decreased in HT patients, but the expression of SENP1 was enhanced compared to healthy controls (HCs). However, compared to the pTg-induced HT rat group, the expression of Foxp3, SUMO-1, and SUMO-2/3 and the proportions of CD4+CD25+Foxp3+ Treg cells were increased, whereas the expression of SENP1 was decreased, in the dioscin-treated group. Furthermore, the SUMOylation of IRF4 was increased after SENP1 was knocked down. The results of our study indicate that dioscin can promote the differentiation of the CD4+CD25+Foxp3+ Treg cells and subsequently upregulate the SUMOylation of IRF4 by downregulating SENP1 expression.
研究领域:
免疫学
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分子生物学
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内分泌学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Xin Qiao,Cheng Qu,Qiming Luo,Yuanzhong Wang,Jie Yang,Hua Yang,Xiaodong Wen
Rhizoma Paridis (RP) with significant anti-tumor and haemostatic effects, has been used as the raw material of many Traditional Chinese preparations. However, its active ingredients are still unclear. The present study aimed to discover bioactive ingredients from RP based on spectrum-relationship and chemometric methods. Firstly, the saponins extract was prepared by phytochemical methods. Furthermore, UHPLC-QTOF-MS and UHPLC-qMS were incorporated to establish an efficient and sensitive method for obtaining the chemical profiles of RP. A total of 34 saponins were characterized in RP and 13 of them were assigned as common peaks in 25 batches of samples. After evaluation of the anti-tumor and haemostatic activities of samples, spectrum-effect relationships were investigated by the grey relational analysis (GRA), orthogonal projections to latent structures (OPLS) and back propagation artificial neural network (BP-ANN). These analyses showed that polyphyllin VII (P27), polyphyllin II (P30), dioscin (P31) and polyphyllin I (P33) play a role in the haemostatic effects of RP whereas polyphyllin VII (P27), dioscin (P31), polyphyllin I (P33), progenin III (P34) were assigned as candidate ingredients accounting for the anti-tumor activity of RP. The anti-tumor and haemostatic activities of these screened ingredients were subsequently verified in vitro . Collectively, the present study established the spectrum-effect relationship mode of RP and discovered the bioactive compounds of RP, which could be also used for exploration of bioactive compounds in herbal medicines, especially for trace compounds.
研究领域:
药理学
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分析化学
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中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）