FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Yuan Chen,Huan Zhang,Xin Feng,Liang Ma,Yuhao Zhang,Hongjie Dai
In this study, the pineapple peel treated with different degrees of delignification was used to isolate lignocellulose nanocrystals (LCNC) by sulfuric acid hydrolysis. Controlling delignification treatments can adjust the morphology and structure of pineapple peel and the retention of lignin, thereby achieving the regulation of the properties of LCNC, such as morphology, crystallinity, hydrophobicity and rheological properties. The results of atomic force microscope (AFM), confocal laser scanning microscopy (CLSM), UV/visible (UV–Vis) spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the presence of lignin in LCNC, showing a rod-like structure with the distribution of lignin. Regulating delignification of pineapple peel can adjust the average length (310 ∼ 460 nm), diameter (19 ∼ 38 nm), crystallinity (61% ∼ 71%) and hydrophobicity (contact angle 84° ∼ 60°) of the obtained LCNC by acid hydrolysis, and influence the performance of its stabilized Pickering emulsions. This work confirms that the properties of LCNC can be controlled through adjusting delignification degree, possessing great significance for the high value utilization of lignocellulosic agricultural waste.
研究领域:
材料科学
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食品科学
·
农业废弃物利用
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产品关联
DOI
PubMed
高影响因子
Genes
·
2025
·Vol. 16
·期 2
·217
·
IF 2.8
Haiyan Shu,Aiping Luan,Hidayat Ullah,Junhu He,You Wang,Chengjie Chen,Qing Wei,Rulin Zhan,Shenghe Chang
Background/Objectives: CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats)-associated protein 9 is now widely used in agriculture and medicine. Off-target effects can lead to unexpected results that may be harmful, and these effects are a common concern in both research and therapeutic applications. Methods: In this study, using pineapple as the gene-editing material, eighteen target sequences with varying numbers of PAM (Protospacer-Adjacent Motif) sites were used to construct gRNA vectors. Fifty mutant lines were generated for each target sequence, and the off-target rates were counted. Results: Selecting sequences with multiple flanking PAM sites as editing targets resulted in a lower off-target rate compared to those with a single PAM site. Target sequences with two 5′-NGG (“N” represents any nucleobase, followed by two guanine “G”) PAM sites at the 3′ end exhibited greater specificity and a higher probability of binding with the Cas9 protein than those only with one 5′-NGG PAM site at the 3′ end. Conversely, although the target sequence with a 5′-NAG PAM site (where “N” is any nucleobase, followed by adenine “A” and guanine “G”) adjacent and upstream of an NGG PAM site had a lower off-target rate compared to sequences with only an NGG PAM site, their off-target rates were still higher than those of sequences with two adjacent 5′-NAG PAM sites. Among the target sequences of pineapple mutant lines (AcACS1,AcOT5,AcCSPE6,AcPKG11A), more deletions than insertions were found. Conclusions: We found that target sequences with multiple flanking PAM sites are more likely to bind with the Cas9 protein and induce mutations. Selecting sequences with multiple flanking PAM sites as editing targets can reduce the off-target effects of the Cas9 enzyme in pineapple. These findings provide a foundation for improving off-target prediction and engineering CRISPR-Cas9 complexes for gene editing.
CAS / MDL:
研究领域:
植物学
·
遗传学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2020
·
IF 1.431
Yiran Liang,Wenxia Wu,Xiaojun Cheng,Jiye Hu
The aim of this study was to investigate the fate, terminal residues and risk assessment of spirotetramat and its four metabolites in pineapple and pineapple pulp samples. To achieve this goal, a simple, efficient and sensitive method based on QuEChERS technique was developed using LC-MS/MS. The limit of quantification was 0.01 mg/kg for all the target analytes with the average recoveries ranging from 77% to 109%. The validated method was successfully applied to investigate the fate and terminal residue of spirotetramat and its four metabolites in pineapple and pineapple pulp samples in China. The dissipation dynamics of spirotetramat in pineapple were investigated according to first-order kinetics and the calculated half-lives were 4.6 and 2.3 days in the two sampling locations. After two or three applications at the doses of 80 to120 mg a.i./kg, the highest spirotetramat residue (sum of spirotetramat and its four metabolites) that detected in pineapple and pineapple pulp samples were 0.203 and 0.064 mg/kg. The dietary risk assessment of spirotetramat in pineapple was assessed by risk quotients and the calculated risk quotient value was found to be far below 100%, indicating a low health risk to the consumers. As there was no recommended MRL value in pineapple in China, these results should be useful for safe and reasonable usage of spirotetramat in pineapple in China.
研究领域:
食品科学
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环境科学
·
毒理学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yihang Zhang,Jing Guo,Jianbin Sun,Fucheng Guan,Jing Tao,Xuecui Song,Zheng Li
Tea bags have been extensively used in the food industry and daily life as an efficient way to pack tea. However, the large pores of the commercial tea bags not only lead to the inner contents of tea bag susceptible to bacteria and moisture but also result in the faster water infusion which is undesired during tea brewing. In this study, the polylactic acid (PLA)-PLA/sodium alginate (SA)/bromelain (BL) bilayer fiber membrane imitating the asymmetric wetting structure of lotus leaf blades was fabricated to avoid the above disadvantages of commercial tea bag. The PLA/SA/BL skin-core nanofiber membrane which imitating the skin-core structure of lotus leaf stems was first prepared as the hydrophilic and support layer, then a hydrophobic PLA layer was deposited on top via electrospinning. The PLA-PLA/SA/BL bilayer fiber membrane had a breaking strength of 5.5 MPa and started to decompose at 260 °C. Using this bilayer membrane , tea bags were designed with a novel structure where the hydrophobic PLA layer was placed in the same direction. The novel structure endow the those tea bags a slow and directional water transfer property. Therefore, the PLA-PLA/SA/BL bilayer fiber membrane has great potential for applications as tea bags.
研究领域:
食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Hongjie Dai,Yue Huang,Huan Zhang,Liang Ma,Huihua Huang,Jihong Wu,Yuhao Zhang
Herein, the hierarchically processed pineapple peel was achieved by water (WT-PP), alkali (AT-PP), bleaching (BT-PP) and bleaching-alkali (PPC) treatments respectively for separate or simultaneous removal of hemicellulose and lignin. These treated pineapple peel were further fabricated into hydrogels (WT-PPH, AT-PPH, BT-PPH and PPCH, respectively) by a facile dissolution-regeneration process in ionic liquid . The structure and Congo red (CR) adsorption ability of the hydrogels were investigated and compared. The adsorption process conformed better to the pseudo-second-order kinetic and Langmuir isotherm models. The maximum adsorption capacity was 114.94 mg/g for WT-PPH, 77.52 mg/g for AT-PPH, 138.89 mg/g for BT-PPH and 75.19 mg/g for PPCH, implying the separate bleaching treatment could improve the CR adsorption capacity more. Moreover, the hydrogels displayed a pH-dependent behavior and good reusability during adsorption process. Based on removal of initial components and simple preparation method, this work would be beneficial for the development of agricultural residues-based biosorbents.
研究领域:
材料科学
·
环境科学
·
农业废弃物利用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Fermentation-Basel
·
2023
·Vol. 9
·期 2
·79
·
IF 3.7
Jiawei Luo,Shan Xiao,Jihui Wang,Bo Wang,Yanxue Cai,Wenfeng Hu
Value-added utilization of pineapple waste is very import for the food industry and environmental protection. In this study, whey protein (2.6%,w/w) was added to pineapple waste in order to make up for the protein deficiency of the raw material and give the final products better flavor characteristics. AutochthonousLactococcus lactisLA5 andHanseniaspora opuntiaeSA2 were used for the co-inoculation of pineapple by-products; during fermentation, the metabolite profiling and microbial community dynamics were investigated. Results showed that the contents of organic acids, total FAAs, total phenolic compounds and flavonoids significantly increased with fermentation, and 152 kinds of peptides were identified in the final products. Relevant analyses demonstrated that dominant strains includingLactococcus lactis,HanseniasporaandSaccharomycesnot only significantly promoted the accumulation of organic acids, total phenols and other active substances, but also inhibited the growth of pathogenic bacteria and further influenced the fermentation process of pineapple waste.Keywords:pineapple by-products;whey protein;metabolites;microbial community;active peptide
研究领域:
食品科学
·
微生物学
·
生物化学
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产品关联
DOI
高影响因子
Materials Today Nano
·
2023
·
IF 10.3
Yangyang Qian,Yudi Kuang,Yazeng Zhang,Yuan Wei,Yijun Liu,Chunyu Wang,Gang Chen
As wearable electronics and wireless communications grow in popularity, high-performance electromagnetic interference (EMI) shielding materials are being explored for reducing serious radiation pollution. In this work, the high aspect ratio and high crystallinity of pineapple leaf nanofibers (PALNF), extracted and separated from the agricultural waste of pineapple leaves, are being considered potential dispersants and binders for assisting in the fabrication of ultrathin, highly flexible and mechanically robust Ti 3 C 2 T x MXene/PALNF EMI shielding films. The “bricks-and-mortars” composite film containing only 50 wt % PALNF exhibits a superior tensile strength (up to 159.6 MPa), fracture strain (up to 6.7%), and toughness (up to 5.8 MJ/m 3 ), which is several times or dozens of times higher than other reported freestanding MXene film. In particular, the composite film with a ratio of 1: 1 displays outstanding specific EMI shielding properties of 6541.1 dB cm 2 g −1 in the X-band at a thickness of only 28 μm. Thus, from the perspective of “waste to wealth” and nacre bionics, recycling waste pineapple leaves through reprocessing into high-value EMI shielding films is a feasible way to reduce environmental problems caused by these wastes and electromagnetic radiation .
研究领域:
材料科学
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环境科学
·
纳米技术
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产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Xinxin Chen,Xiang Wang,Siyu Wang,Xiuhang Zhang,Jiaao Yu,Ce Wang
There has been a recent increase in research interest regarding the development of wound dressings containing bioactive compounds capable of improving outcomes for complex healing needs. In the present study, we describe the generation of bromelain immobilized eletrospun poly(ε-caprolactone) (PCL) fibers (BrPDA-PCL fibers) using the dopamine-assisted co-deposition strategy. We wanted to combine the structural advantage of electrospun fiber and the activity of bromelain and PDA to develop functional wound dressings. We found that bromelain activity could be better stabilized when via its immobilization on electrospun fibers. The resultant BrPDA-PCL fibers exhibited promising properties including optimal mechanical stability, wettability, and rates of water vapor transmission. In addition, these BrPDA-PCL fibers were biocompatible, allowing for effective cellular adhesion and proliferation. The results of zone of inhibition testing further confirmed that these fibers achieved effective antibacterial activity against Escherichia coli and Staphylococcus aureus . When used in vivo, as compared with PCL fibers or control animals the BrPDA-PCL fibers enhanced wound healing rates while reducing associated inflammation. As such, these results indicate that these biocompatible BrPDA-PCL fibers exhibit desirable physicochemical properties making them ideal for use as a wound dressing to enhance the repair of full-thickness wounds to the skin.
研究领域:
生物材料学
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医学工程
·
组织工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2019
·Vol. 43
·期 9
·e12976
·
IF 1.358
Peng Luo,Juan Han,Yuan Li,Yun Wang,Li Wang,Liang Ni
Bromelain has wide applications in different industries, such as food, textile, and medicine. Traditional approaches for bromelain separation and purification from solution still have many problems, including unsatisfactory binding efficiency, time-consuming operation, and costly equipment. In the present study, a new type of dendritic polymer-based magnetic carrier (GO@Fe 3 O 4 @PEI-Cu 2+ ) was first prepared for bromelain separation and purification in solution. The histidine existing in bromelain could bind to Cu 2+ cations adsorbed on the surface of the magnetic carrier, and the magnetic carrier showed excellent performance for bromelain separation and purification in solution, with the adsorption capacity up to 357 mg/g. The magnetic carrier also exhibited excellent property in the aspect of recyclability. It was found that the magnetic carrier also presented desirable performance for the separation and purification of bromelain from the crude extract of pineapple peel, and the bromelain structure remained intact before and after elution process. Practical applications Considering many advantages of bromelain in the applications of pharmaceutical and food industries, this study is aimed at presenting a novel magnetic carrier with high stability and fabulous performance for bromelain separation and purification in solution and achieving the practical application that the magnetic carrier can efficiently separate bromelain from the crude extract of pineapple peel.
研究领域:
生物化学
·
材料科学
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DOI
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 49
·495708
·
IF 3.874
Lu Tang,Jing Liao,Hongjie Dai,Yushan Liu,Huihua Huang
Pineapple, as a world-famous tropical fruit, is also prone to produce by-products rich in cellulose. In this study, different sections of pineapple, including pineapple core (PC), pineapple pulp (PPu), pineapple leaf (PL) and pineapple peel (PPe) were used for production of pineapple cellulose nanocrystals (PCNCs) by sulfuric acid hydrolysis. The crystallinity of PCNCs from PC, PPu, PL and PPe were 57.81%, 55.68%, 59.19% and 53.58%, respectively, and the thermal stability of PCNCs in order was PC > PL > PPe > PPu. The prepared PCNCs from PC, PPu, PL and PPe were needle like structure at the average aspect ratios of 14.2, 5.6, 5.5, and 14.8, respectively. Additionally, the differences in the structure and properties of PCNCs affected the stability of the prepared Pickering emulsions, which ranked as PPu > PPe > PL > PC. The Pickering emulsions stabilized by PCNCs prepared from PPu could be stored stably for more than 50 d. These results show the differences of PCNCs from four sections of pineapple, and provide isolated raw material selection for the further application of PCNCs.
研究领域:
食品科学
·
材料科学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xiangrong Li,Wanqing Xu,Xianfei Liu,Zhizhi Song,Zheng Li,Zihan Jia
In this study, the interaction mechanism of papain and bromelain with apigenin and luteolin in binary and ternary systems was compared and analyzed by spectroscopic methods, molecular docking, and molecular dynamics simulation. Additionally, the effects of apigenin and/or luteolin on the conformational changes and enzymatic activity of papain and bromelain were investigated. The results show that apigenin and/or luteolin exhibit a moderate binding affinity with papain/bromelain, with the binding constants K a in the range of 10 4 –10 5 L mol −1 . The order of addition of apigenin and luteolin significantly influenced their binding affinities to the enzymes. The main non-covalent forces of the papain-luteolin (first)-apigenin ternary system are hydrogen bonds and Van der Waals forces. Hydrophobic interactions, electrostatic forces and hydrogen bonds are the main non-covalent forces of other binary and ternary systems. These systems differentially alter the microenvironment of Trp and Tyr residues, secondary and tertiary structures, and enzymatic activity. Molecular docking and dynamics simulations identified specific binding sites of apigenin and/or luteolin on papain/bromelain, corroborating experimental findings. Notably, the ternary system exhibits a stronger inhibitory effect on papain activity compared to binary systems.
研究领域:
生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2016
·
IF 3.449
Rui Li,Linlin Ding,Zhaoliang Wu,Yanji Wang,Wei Liu,Yanyan Wang
For reducing the costs of separating bromelain from its crude extract, a two-stage foam fractionation technology with β -cyclodextrin as an activity protector was developed using the foam fractionation column with vertical sieve trays (VSTs). By studying the effects of VSTs, volumetric air flowrate, β -cyclodextrin concentration, aeration time and temperature, the two-stage foam fractionation technology were optimized. The results show that VSTs effectively improved the activity recovery by enhancing the adsorption of bromelain at the gas-liquid interface while β -cyclodextrin significantly reduced the activity loss induced by the gas-liquid interface. At β -cyclodextrin concentration 0.60 mg/mL, a high specific activity of 165.6 U/mg, 7.4 folds of that in the crude extract, was obtained with an activity recovery of 45.2% by using the optimized two-stage foam fractionation technology. Compared to those without β -cyclodextrin, activity recovery and specific activity obtained satisfactory relative increases of 34.1% and 110.7%, respectively.
研究领域:
生物工程
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）