This work presents a method for the establishment of a highly sensitive and selective nalidixic acid and S 2 O 8 2− (NA/S 2 O 8 2− ) electrochemiluminescence (ECL) system using isonicotinic (INH) as a co-reaction accelerator. After optimizing the experimental conditions including scan rate, pH value of buffer solution, concentration, etc., the chemiluminescence mechanism of the system was studied in detail using fluorescence spectroscopy, ECL spectroscopy and cyclic voltammetry (CV). The results show that INH is an efficient co-reaction accelerator, which interacts with the co-reactant S 2 O 8 2− but not the luminophore NA to promote the production of more oxidant SO 4 ·– , which then further reacts with NA to generate excited state species NA* with strong ECL signal, indicating that co-reaction accelerators can provide a simple, efficient, and low-cost method for signal amplification. Based on the great ECL enhancement of INH for the NA/S 2 O 8 2− system, under the optimal experimental conditions, highly sensitive and selective electrochemiluminescence detection of tetraethylenepentamine (TEPA) was achieved.
Su Han Wang,Ning Ma,Xiao Huan Zang,Shuai Hua Zhang,Jian Ping Wang,Ning Peng Wu
A graphitic carbon nitride was synthesised and nalidixic acid (NA) based molecularly imprinted microspheres (MIMs) were polymerised on its surface to create a composite material. After characterisation and evaluation of its absorption ability, the composite was used to prepare a solid-phase extraction (SPE) cartridge for purification of fluoroquinolones in chicken muscle, analysed by ultra-performance liquid chromatography. The cartridge showed high absorption capacities (378–559 μg) and high recoveries (92.1–99.4%) for eight fluoroquinolones, and could be reused at least 20 times. The limits of detection for the 8 drugs were 0.2–0.8 ng g−1, and recoveries from standard fortified blank chicken muscle samples were 71.9–96.8%. This is the first study reporting the use of molecularly imprinted graphitic carbon nitride composite to determine the residue of veterinary drug in foods of animal origin.
危险品化学品经营许可证（带存储）