PEST MANAGEMENT SCIENCE
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2018
·Vol. 75
·期 4
·986-997
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IF 3.249
Lulu Dai,Haiming Gao,Jiaqi Ye,Danyang Fu,Yaya Sun,Hui Chen
BACKGROUND Bark beetles rely on detoxifying enzymes to resist the defensive terpenoids of their host trees. Research on carboxylesterases (CarEs) has focused on their multiple functions in the metabolic detoxification of pesticides and plant allelochemicals, drug resistance, and juvenile hormone and pheromone degradation. RESULT We identified eight new CarE genes in the Chinese white pine beetle ( Dendroctonus armandi ) and carried out bioinformatics analysis on the deduced full-length amino acid sequences. Differential transcript levels of CarE genes were observed between sexes; within these levels, significant differences were found among the different development stages, and between insects fed on the phloem of Pinus armandi and exposed to five stimuli [(−)-α-pinene, (−)-β-pinene, (+)-3-carene, limonene and turpentine] at 8 and 24 h. CONCLUSION Transcription levels of CarE genes suggest some relationship with the detoxification of terpenoids released by host trees. The functions of bark beetle esterase are mainly in hydrolyzing the host chemical defense and degrading odorant molecules during host selection and colonization. © 2018 Society of Chemical Industry
研究领域:
昆虫学
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分子生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
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2024
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IF 2.3
Yu-Ting Wang,Yuan Dong,Fang Wang,Shuai-Ming Zhu,Yang Zhang,Yu Yang,Rui Xu,Cai-Hong Dong,You-Zhi Zhang,Chang-Wei Li
Four previously undescribed caryophyllene sesquiterpenoids ( 1 – 4 ) along with one known drimane sesquiterpenoid ( 5 ) were isolated from the fermentation products of the mushroom Hypholoma capnoides 819. The structures of 1–4 with rare tricyclic skeleton (6/5/4) or bicyclic skeletons (9/4) were determined on the basis of comprehensive spectroscopic data analysis, calculated NMR, ORD data, and their biosynthetic pathways. Compounds 1–5 only showed very weak cytotoxicity toward mouse microglial cells (BV2), breast cancer cells (MCF-7), and human lung cancer cells (A549) at 40 µM. In addition, none of the compounds demonstrated antibacterial activity against Staphylococcus aureus or Escherichia coli at 128 µg/mL. Graphical Four previously undescribed caryophyllene sesquiterpenoids (1–4) along with one known drimane sesquiterpenoid (5) were isolated from the fermentation products of the mushroom Hypholoma capnoides 819.
研究领域:
天然产物化学
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药物化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2018
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IF 6.14
Chao Ju,Meng Wang,Yong Huang,Yunming Fang,Tianwei Tan
Biojet fuel production has attracted a lot of research interest due to the double threats of fuel shortage and environmental concerns. This paper works on the high-quality jet fuel blend production by oxygen-containing terpenoids hydroprocessing. Hydroprocessing of three typical oxygen-containing terpenoids (geranyl acetone, nerolidol, and geraniol) over Pt/Al2O3 was first studied. The results indicate that the coexistence of branched carbon backbone, carbon–carbon double bond, and oxygen-containing functional group makes the terpenoids highly reactive during the hydroprocessing. The Pt/Al2O3 catalyst, which is considered to have a mild acidity, is still too active in terpenoids hydrogenation and results in large heat release and extensive side reactions. Hence, Pt supported on a support with a milder acidity and better diffusion property was proposed to build a suitable terpenoids hydroprocessing catalyst. Pt/Al-MCFs (MCF: mesocellular silica foam) were then successfully prepared, characterized, and tested in this reaction. Acidic sites could be introduced into MCFs by Al grafting of the pure silica MCF. The acidities of MCF supports, and consequently Pt/MCFs, could be fine-tuned by the Si/Al ratio in the resulting supports. Pt/Al-MCF-20 with a milder acidity than Pt/Al2O3 shows an excellent performance in geranyl acetone hydroprocessing with complete deoxygenation, limited side reaction, and high stability. Characterization results of Pt/Al-MCFs and Pt/MCF indicate that Al introduction is critical for the stable performance. The products obtained from geranyl acetone hydroprocessing over Pt/Al-MCF-20 are highly promising as jet fuel or jet fuel blend due to their multibranched alkane and alkyl-cycloalkane nature, which meets all the specifications of ASTM 7566 in the desired freezing point (−72 °C), density (0.80 g/mL), flash point (51 °C), heat of combustion (45 MJ/kg), and aromatics content (<1%).
研究领域:
能源科学
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催化化学
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产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
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2015
·Vol. 72
·期 4
·816-827
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IF 2.694
Lulu Dai,Junning Ma,Mingyuan Ma,Haoqiang Zhang,Qi Shi,Ranran Zhang,Hui Chen
BACKGROUND Bark beetles rely on their detoxifying enzymes to resist the defensive terpenoids of host trees. Glutathione S -transferases (GSTs) conjugate xenobiotic compounds with a glutathione moiety (GSH) and often work in tandem with cytochromes P450 or other enzymes that aid in the detoxification, sequestration or excretion of toxic compounds. RESULT We identified nine new GST genes in the Chinese white pine beetle ( Dendroctonus armandi ) and carried out a bioinformatics analysis on the deduced full-length amino acid sequences. These genes belong to four different classes (epsilon, sigma, omega and theta). Differential transcript levels of each class of GST genes were observed between sexes, and, within these levels, significant differences were found among the different adult substages that were fed phloem of Pinus armandi and exposed to six stimuli [(±)- α -pinene, (−)- α -pinene, (−)- β -pinene, (+)-3-carene, (±)-limonene and turpentine] at 8 and 24 h. CONCLUSION The increased transcription levels of GST genes suggested that they have some relationship with the detoxification of terpenoids that are released by host trees. The mediating oxidative stress that is caused by monoterpene might be the main role of the bark beetle GSTs. © 2015 Society of Chemical Industry
研究领域:
昆虫学
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分子生物学
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生物化学
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DOI
PubMed
高影响因子
Polymers
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2025
·Vol. 17
·期 17
·2421
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IF 4.9
Lina Tang,Qian Chen,Liming Zhu,Xiaorui Liu,Xianwu Zou,Yuejin Fu,Bo Liu
Wood, as the oldest natural polymer composite material on Earth, holds significant importance in the era of carbon neutrality and serves as an irreplaceable core material in the furniture and construction industries. As a primary raw material for furniture, wood-based lignocellulosic boards have drawn increasing consumer attention due to their odor characteristics. In order to achieve the determination of odor compounds in lignocellulose-based panels, this study established a method combining dynamic headspace sampling (DHS), gas chromatography–mass spectrometry (GC–MS), and odor activity value (OAV) analysis. To address the wide concentration range of odor compounds in lignocellulose-based panels, a three-level standard curve was established to meet the detection of odor substances in common lignocellulose-based panels. The favorable conditions for each factor were as follows: sheet-shaped samples, TENAX-TA adsorbent, 20 mL headspace vials, and a split ratio of 25:1. The method demonstrated good linearity within the range of 0.002–15 mg/m3, with recovery rates ranging from 94.74% to 103.44%. The method was applied to analyze commercially available particleboard, fiberboard, and plywood. A total of 33 odor components were detected. The results indicated that aldehyde contributed significantly to the odor of particleboard, acids were the main contributors to the odor of fiberboard, and terpenes dominated the odor of plywood. The established method is suitable for the qualitative and quantitative analysis of odor compounds in lignocellulose-based panels and provides reliable technical support for tracing, identifying, and controlling odors in these materials.
研究领域:
木材科学与技术
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分析化学
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室内空气质量
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材料气味分析
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可持续材料
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2024
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IF 11
Xingran Kou,Xiwei Xu,Nan Gao,Yunchong Zhang,Xin Huang,Feng Chen,Qinfei Ke,Qingran Meng
Supramolecular chemistry of cyclodextrins (CDs) inclusion complexes is a hot research topic at present, but current studies often focus on the host-guest interaction between CDs and hydrophobic substances, while ignoring the self-assembly behavior of the host and guest during the inclusion process. Based on the spatial structures, six representative terpenes were selected in this study, and the stoichiometric ratio between terpenes and β-cyclodextrin (β-CD) was investigated by isothermal titration calorimetry (ITC) method. Meanwhile, the constructed inclusion complexes (ICs) were characterized by nuclear magnetic resonance , X-ray diffraction, scanning electron microscope , etc. On this basis, the assembly process of β-CD and terpenes was investigated firstly by molecular simulation method, and the inclusion process between β-CD and terpenes was further studied in terms of the formation of individual ICs and ICs aggregates. Results showed that hydrophobic interaction and van der Waals force were the main driving forces of the reaction between terpenes and β-CD, and the formation of ICs, β-CD molecule aggregation, terpene aggregation, and ICs aggregation occurred simultaneously during the inclusion process of terpenes and β-CD. To sum up, this study may provide certain thinking and reference for the subsequent supramolecular chemical research on CDs.
研究领域:
超分子化学
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环糊精化学
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主客体化学
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自组装
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物理化学
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分子识别
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有机化学
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DOI
高影响因子
Frontiers in Microbiology
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2025
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IF 4.5
Xiao Hong Tang,Yan Ding,Ke Zhong,Yu Xia Sun,Xiao Mei Han,Zhi Yu Li,Rui Rui Li
The majority of aroma precursors in grapes exist as odorless glycosidic conjugates, which can be hydrolyzed by β-glucosidase to release free volatile aroma compounds, thereby enhancing the aromatic quality of wine. This study aimed to screen and characterize indigenous non-Saccharomyces cerevisiae strains with β-glucosidase activity for their potential to enhance terpenoid aroma compounds during wine fermentation. Grapes collected from 14 vineyard plots in the Penglai Wine Region (China) underwent spontaneous fermentation and yielded 203 single colonies. Among them, 85 strains of non-Saccharomyces cerevisiae were initially screened based on the geniposide chromogenic method, which were classified into 7 genera and 16 species. Nine high-performance strains were subsequently selected for small-scale fermentation trials using Petit Manseng grape juice. The physicochemical parameters of wines were analyzed by high-performance liquid chromatography (HPLC), while volatile aroma compounds were quantified using a headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry (HS-SPME-GC–MS). The results revealed that Starmerella bacillaris CGCD1-9, Pichia fermentans CGCD1-4, and Zygosaccharomyces bailii JDCD01 significantly enhanced glycerol production, which might contribute to improved wine sensory quality. Four Hanseniaspora yeast strains exhibited the ability to increase ethyl acetate content. Among these strains, Hanseniaspora vineae CGCD1-1, Hanseniaspora uvarum CGCD1-3, and Hanseniaspora opuntiae CGCD1-7 showed a pronounced tendency to elevate geraniol levels, whereas Hanseniaspora occidentalis CGCD1-5 selectively promoted the biosynthesis of β-damascenone and 2-furanmethyl acetate. Zygosaccharomyces bailii JDCD01 primarily increased the levels of isobutanol and phenylethyl alcohol, while also exhibiting a slight enhancement in terpenoid production. Notably, S. bacillaris CGCD1-9 significantly enhanced five key terpenoids, namely, linalool, geraniol, citronellol, α-terpineol, and nerolidol, yielding the highest total terpene content among all strains, while increasing the content of alcohols (less than JDCD01). In contrast, Issatchenkia terricola SIVE4101 and Metschnikowia andauensis LFSY0-17 showed preferential accumulation of β-damascenone and total furans, with the former also significantly increasing the acetate ester content. This study provides new ideas and theoretical support for r developing targeted co-inoculation protocols with S. cerevisiae to achieve precise modulation of wine terpenoid profiles.
研究领域:
微生物学
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酿酒学
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食品科学
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生物技术
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分析化学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）