Algal Research-Biomass Biofuels and Bioproducts
·
2020
·
IF 4.008
Song Cao,Fei Teng,Ting Wang,Xingxing Li,Jiahui Lv,Zhonghua Cai,Yi Tao
Microalgae immobilization has been developed as a prospective method to improve the culturing and harvesting of microalgae cells in wastewater; it is also an effective method to control membrane fouling in an algal membrane system. In this study, a continuous immobilized microalgae membrane bioreactor with alginate-immobilized Chlorella vulgaris beads was developed for investigating the progress on nutrient removal and the characteristics of membrane fouling through synthetic wastewater treatment . High initial total biomass (more than 0.52 g/L) led to significant fluctuations in nutrient removal. The growth pattern of microalgae cells within the beads was explored for a better understanding of the fate of immobilized microalgae. The results showed that a moderate initial cell density of microalgae cells (7.4 × 10 5 cell/bead) in the immobilized beads achieved relatively higher specific growth rate than that of a high density, and higher nutrient removal efficiency than that of a low density. The fluorescence microscopy results of a hemisphere cut from the immobilized beads indicated that a shading layer was formed with high-density microalgae cells after a 2-day cultivation. Thus, setting a proper initial cell density of microalgae in immobilized beads was vital to achieve better performance on nutrient removal and microalgae cultivation and harvesting in the continuous operation of a reactor. Immobilization is highly effective for retarding membrane fouling through the reduction in suspended microalgal cells, mitigating the release of macromolecule algal organic matter from the immobilized beads, and regular replacement of the immobilized beads. The results and optimized directions discussed in this paper provide integrated clues and suggestions for future microalgal immobilization-related development.
研究领域:
环境生物学
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废水处理工程
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Xin Lv,Songhe Zhang,Shaozhuang Guo,Xiuren Hu,Hezhou Chen,Zheng Qiu,Yuexiang Gao,Aiyu Qu
The fate and effects of sodium dodecyl benzene sulfonate (SDBS) in sewage treatment plants effluents on nutrients and submerged macrophytes are far from clear in wetlands. This study conducted a 24-day experiment to investigate changes in nutrients and epiphytic biofilm of Hydrilla verticillata in wetlands receiving effluents with 0.5, 2 and 5 mg L −1 SDBS. The decrease of SDBS in overlying water followed pseudo-first-order kinetic equation, with over 80 % of SDBS removal achieved. 2 and 5 mg L −1 SDBS decreased nutrient removal efficiency, induced oxidative stress response and damaged cells of H. verticillata . SDBS altered bacterial and eukaryotic community diversity. 0.5 mg L −1 SDBS can promote carbon fixation and methane oxidation of microorganisms. Network analysis revealed that 0.5 mg L −1 SDBS decreased the stability of epiphytic ecosystems. Mantel tests indicated significant influences of SDBS, temperature, and total nitrogen on epiphytic microbial communities.
研究领域:
环境科学
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生态学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 3
·410
·
IF 4.7
Haining Guan,Yanli Tian,Chunmei Feng,Siqi Leng,Shifa Zhao,Dengyong Liu,Xiaoqin Diao
The protein and fat in chicken skeleton can be emulsified in a boiling state to form milky white chicken soup. White chicken soup has a delicious taste, good nutritional value, a beautiful color, and volatile flavor compounds. However, cooking time significantly impacts the quality of white chicken soup. Herein, we investigated the influence of cooking time (30, 60, 90, 120, 150, 180, and 210 min) on the migration of nutrient substances and characteristics changes in white chicken soup from chicken skeletons. The results showed that nutrients such as total lipids, water-soluble protein, total sugars, solid matter, and oligopeptides in the chicken skeletons’ tissue continuously migrated into the soup during the cooking process. The total nutrient content in the chicken soup was highest after cooking for 180 min. Simultaneously, the white chicken soup obtained after cooking for 180 min had low interfacial tension and high whiteness, viscosity, and storage stability. The high stability index was associated with increased ζ potential and decreased particle size. The contact angle analysis results also indicated that the stability of the white chicken soup was improved when the cooking time reached 180 min. This research provides basic information for the production of high-quality white chicken soup.
研究领域:
食品科学
·
营养学
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DOI
PubMed
高影响因子
JOURNAL OF ANIMAL SCIENCE
·
2022
·Vol. 100
·期 11
·
IF 3.338
Pan Long,Feng Shaoxuan,Li Wang,Zhu Weiyun
High-tannin sorghum grain (HTS) has been previously proved to contain lower apparent total tract digestibility (ATTD) of nutrients than low-tannin sorghum grain (LTS) for pigs. This study was conducted to identify in which segments (foregut or hindgut) of the intestinal tract of pigs the digestion of nutrients was mostly influenced, and to compare the digestion and fermentation characteristics of LTS and HTS in the porcine gastrointestinal tract. In experiment 1, HTS and LTS were digested by porcine pepsin and pancreatin to simulate small intestine digestion, and subsequently the undigested residues were incubated with fresh pig cecal digesta as inoculums for 48 h to simulate the porcine large intestine fermentation in vitro. The results revealed that the in vitro digestibility of air-dry matter, gross energy (GE), and crude protein (CP) was lower (P < 0.05) in HTS than that in LTS, regardless of the simulated small intestine digestion or large intestine fermentation. The enzymatically unhydrolyzed residue of HTS decreased the accumulative gas production excluding the first 3 h and the short-chain fatty acid concentration including acetic acid, propionic acid, and butyric acid in the fermented solutions (P < 0.05), although it provided more nutrients as fermentation substrates (P < 0.05). In experiment 2, 12 crossbred barrows (25.5 ± 2.5 kg body weight) with a T-cannula inserted in the distal ileum were randomly allotted to two diets (N = 6) to determine nutrient digestibility in the foregut (AID, apparent ileal digestibility) and in the hindgut of pigs (HGD, hindgut disappearance). The study lasted 10 d, with a 5 d adaption to the diets followed by a 3 d collection of feces and then a 2 d collection of ileal digesta. Diets included 96.6% HTS or LTS as the only source of dietary energy and nitrogen. The AID and ATTD of dry matter, GE, and CP in HTS were lower than those in LTS (P < 0.05). There was no difference in HGD of nutrients between LTS and HTS. Eight out of fifteen amino acids in HTS had lower AID values (P < 0.05). In conclusion, HTS provided lower small intestine digestibility of nutrients and lower large intestine fermentation parameters, implying that condensed tannins in sorghum grain may impede the nutrient digestibility in the foregut and limit the fermentability in the hindgut segment of pigs. Hence, digestion and fermentation characteristics of sorghum grain may vary depending on the condensed tannins.
研究领域:
动物营养学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ARCHIVES OF ANIMAL NUTRITION
·
2015
·
IF 1
Shixin Lin,Chen Wei,Guangyong Zhao,Tingting Zhang,Kai Yang
The objectives of the trial were to compare the effects of supplementing rare earth elements (REE) lanthanum (La), cerium (Ce) and praseodymium (Pr) on rumen fermentation, nutrient digestion, methane (CH4) production, nitrogen (N) balance and plasma biochemical parameters in beef cattle. Four Simmental male cattle, aged 12 months, with initial average liveweight of 333 ± 9 kg and fitted with rumen cannulas, were fed with a basal ration composed of concentrate mixture and maize silage. Animals received a basal ration without adding REE (Control) or three treatments, i.e. supplementing LaCl3, CeCl3 or PrCl3 at 204 mg/kg DM to the basal ration, respectively, which were allocated in a 4 × 4 Latin square design. Each experimental period lasted 15 d, consisting of 12 d for pre-treatment and three subsequent days for sampling. Results showed that all tested levels of REE tended to increase neutral detergent fibre digestibility (p = 0.064) and tended to decrease rumen CH4 production (p = 0.056). Supplementing LaCl3 and CeCl3 decreased total N excretion and urinary N excretion, increased N retention (p < 0.05), tended to increase total urinary purine derivatives (PD) (p = 0.053) and microbial N flow (p = 0.095), whereas supplementing PrCl3 did not affect N retention, urinary PD and microbial N flow. No differences were found in the effects of nutrient digestibility, CH4 production and plasma biochemical parameters among LaCl3, CeCl3 and PrCl3. Further trials using graded levels of LaCl3, CeCl3 and PrCl3 in a wide range are needed to obtain more pronounced results for comparing effects of La, Ce and Pr on rumen fermentation and nutrient digestion in beef cattle.
研究领域:
动物营养学
·
畜牧学
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产品关联
DOI
PubMed
高影响因子
APPLIED OPTICS
·
2022
·Vol. 61
·期 12
·3400-3408
·
IF 1.905
Haodong Zhang,Jing Luo,Jing Luo,Saimei Hou,Zhanpeng Xu,Julian Evans,Sailing He,Sailing He
Incoherent broadband cavity-enhanced absorption spectroscopy (IBBCEAS) can achieve sensitive measurements at trace concentrations for liquid phase marine samples. The IBBCEAS system consists of a cavity-enhancement module (CEM) and a transmission hyperspectral module (THM). The CEM has cavity-enhancement factors up to 78 at 550 nm. Measurements were obtained over a wide wavelength range (420–640 nm) with a halogen lamp, and the optical cavity was formed by two concave highly reflective mirrors (${\rm{R}} = {0.99}$). The minimum detectable absorption coefficient ${\alpha _{{\min}}}$ of ${7.3} \times {{1}}{{{0}}^{- 7}}\;{\rm{c}}{{\rm{m}}^{- 1}}$ at 550 nm corresponds to a limit of detection for nutrients of 780 pM. The spectral resolution of the THM is 3 nm in the wavelength range of 400 to 750 nm. We performed the IBBCEAS measurements for biological and chemical substances, including nutrients, microalgae, and Cy5 dye. The concentrations of nutrients in a deionized water environment and artificial seawater environment were measured at nanomolar levels; the concentration of microalgae phaeocystis was detected with ${3.46} \times {{1}}{{{0}}^4}/{\rm{mL}}$, and fluorescence substances such as Cy5 dye could be measured at 0.03 mg/L. Experimental results show that the IBBCEAS system has the capability for sensitive measurements of biological and chemical substances and has strong potential for in situ ecological marine environmental monitoring function.
研究领域:
分析化学
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海洋科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2025
·
IF 8.4
Jun Xiao,Juan Huang,Yucheng Chen,Ying Wang,Xiuwen Qian,Dengping Liu,Yuan Cao
Constructed wetland (CW) serve as the final ecological barrier for hazardous materials entering the natural water environment. Due to the ecological toxicity and difficult bioutilization characteristics of perfluorooctanoic acid (PFOA) itself, CW technology faces great challenges in the field of PFOA remediation. In this study, nano zero-valent iron (nZVI) was introduced into CWs to explore the mechanism of the synergistic removal of PFOA and nutrients in nZVI-CW system. The results indicated that the addition of 10 mg/L nZVI improved the removal efficiency of CW for 1 and 10 mg/L PFOA, with an average removal rate increased by 3.53–8.70%. The transformation products in CW effluents were qualitatively detected using HPLC-Q-TOF-MS, suggesting that the degradation of PFOA may involve decarboxylation, hydrolysis, redox, elimination, substitution and intramolecular rearrangement processes. The presence of nZVI enhanced the average removal rates of NH 4 + -N, NO 3 − -N and TP by 2.78–18.4% in CWs. The increase in key substrate enzyme activity confirmed the stimulating effect of nZVI on microbial activity. The addition of nZVI facilitated the growth and enrichment of hydroautotrophic denitrifying bacteria, nitrat-dependent iron-oxidizing bacteria, and dissimilatory iron-reducing bacteria. Two types of dissimilatory iron-reducing bacteria ( Geobacter and Acinetobacter ) may be potential PFOA-degrading bacteria. Additionally, signaling pathways related to carbohydrate metabolism, energy metabolism, and xenobiotic degradation and metabolism exhibited higher abundance in the nZVI treated groups.
研究领域:
环境科学
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生态学
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微生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 28
·期 13
·15954-15965
·
IF 3.056
Ji Xiyan,Luo Xin,Zhang Jibiao,Huang Deying
Chlorella vulgaris and Bacillus licheniformis consortium was added to synthetic wastewater with exogenous vitamin B 12 . In the presence of 100 ng/L vitamin B 12 , removal efficiencies of TN, NH 3 -N, PO 4 3— P, and COD were 80.1%, 76.8%, 87.9%, and 76.7%, respectively. The functional groups on the cell surface of the consortium, including -NH, -CH 3 , C=O, C=C, and P-O-C, increased with 100 ng/L vitamin B 12 . These functional groups improved the biological adsorption of the consortium; however, higher concentrations of vitamin B 12 resulted in an occlusion of the functional groups. Furthermore, there were 5 significantly enriched protein pathways, namely carbon fixation in photosynthetic organisms; amino acid metabolic pathways; the pathway of one carbon pool by folate; nitrogen metabolism; and photosynthesis. Most proteins in these pathways were upregulated, which enhanced carbon fixation and photosynthesis in the algal cells. Simultaneously, B 12 promoted significant upregulation of proteins associated with the quorum-sensing pathway, which promoted the interaction between algae and bacteria.
研究领域:
环境科学
·
微生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Shuang Liu,Yong-Liang Yu,Jian-Hua Wang
Long-term and multipoint monitoring is very important to evaluate the safety of water environment. Atomic fluorescence spectrometer (AFS) is generally used for analyzing metals or metalloids in water quality monitoring. In the present work, for the first time, AFS is employed for sensitive monitoring of nitrogen nutrients, i.e., NO 2 − , NO 3 − and NH 4 + , with nitric oxide (NO) vapor generation. By taking advantage of the different vapor generation conditions for NO 2 − , NO 3 − and NH 4 + , a unit integrating with sampling, heating, cooling, gas-liquid separation and moisture removal is employed for controlling the vapor generation process, by reacting with either iodide or titanium (III). An antimony hollow cathode lamp is used for the excitation of NO to produce fluorescence emission at 193–320 nm. Linear calibration graphs are obtained within 0.02–2, 0.1–20, 0.2–1 mg L −1 for NO 2 − , NO 3 − and NH 4 + , along with detection limits of 0.006, 0.03, 0.06 mg L −1 , respectively. The new analytical strategy obviously broadens the application of AFS, and it is well demonstrated by the determination of NO 2 − , NO 3 − and NH 4 + in certified reference materials as well as various real water samples, providing competitive sensitivity with the current techniques for nutrient monitoring in environmental water.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RESOURCES CONSERVATION AND RECYCLING
·
2022
·
IF 13.716
Qing Jiang,Jia Liu,Xiangru Song,Ye Qiu,Jianliang Xue,Yuqiang Shao,Yujie Feng
Human urine contains abundant nutrients, while separately collecting urine can significantly alleviate nutrient loads in existing wastewater treatment facilities. Herein,0020a novel forward osmosis integrated bioelectro-concentration and recovery system (OsBCRS) was developed to recover nutrient ions from urine. NH 4 + and PO 4 3– migrated out from urine and enriched in the internal chamber of OsBCRS driven by the self-generated electric field, which were further concentrated by water extraction from the integrated forward osmosis (FO) process, and finally recovered as struvite. The current density of OsBCRS reached 27.3 A m –3 , which in turn drove the migration of NH 4 + -N (93.7 ± 1.6%) and PO 4 3– -P (94.6 ± 0.8%) out from urine, and these separated nutrients were enriched by 9.4–9.5 times in the internal chambers. Subsequently, these enriched nutrients in the catholyte were 3.2-fold concentrated with 68.6% of volume reduction by FO. The system removed up to 83.4% of NH 4 + -N and 88.8% of PO 4 3– -P, most of which were recovered as struvite taking advantage of reverse-fluxed Mg 2+ . The recovered struvite exhibited similar crystal structure and composition to that of standard struvite. The preliminary operating cost analysis of OsBCRS demonstrated that the revenue, including electricity generation and struvite recovery, could offset the operating cost. This study demonstrated highly efficient enrichment and recovery of nutrient ions from human urine by OsBCRS in a cost-effective way, and would encourage further exploration for nutrients recovery from wastes.
研究领域:
环境工程
·
废水处理
·
资源回收
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Zhufang Wang,Yinglong Su,Jundong Zhu,Dong Wu,Bing Xie
Microplastics (MPs) were continuously transported to wastewater treatment systems and accumulated in sludge constantly, potentially affecting systems function and co-occurrent contaminants fate. However, previous studies were based on acute exposure of MPs, which could not reflect the dynamics of MPs accumulation. Herein, this study firstly raised a more realistic method to evaluate the practical impacts of MPs on systems purification efficiency and antibiotic resistance genes (ARGs) fate. Continuous exposure of MPs did not pose negative effects on nutrients removal, but significantly changed the occurrence patterns of ARGs. ARGs abundances increased by 42.8 % and 54.3 % when exposed to millimeter-size MPs (mm-MPs) polyamide and polyethylene terephthalate , but increased by 31.3 % and 39.4 % to micron-size MPs (μm-MPs), respectively. Thus, mm-MPs posed severer effects on ARGs than μm-MPs. Further, mm-MPs surface properties were obviously altered after long-term exposure (higher specific surface area and O-containing species), which benefited microbes attachment. More importantly, more taxa linkages and changed topological properties (higher average degree and average weight) of co-occurrent network were observed in sludge with mm-MPs than with μm-MPs, as well as totally different potential host bacteria of ARGs. Rough surface of MPs and closer relations between ARGs and bacteria taxa contributed to the propagation of ARGs, which accounted for the observed higher ARGs abundances of mm-MPs. This study demonstrated that long-term accumulation of MPs in wastewater treatment systems affected ARGs fate, and mm-MPs caused severer risk due to their enrichment of ARGs. The results would promote the understanding of MPs real environmental behavior and influences.
研究领域:
环境科学
·
微生物学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Jinhua Cao,Fanzhen Chen,Zheng Fang,Yue Gu,Hao Wang,Jingfang Lu,Yanmeng Bi,Shaopo Wang,Wenli Huang,Fansheng Meng
In this study modified microalgal-bacterial granular sludge (MBGS) was constructed and employed to compare the performance for treating 1%-5% saline wastewater with aerobic granular sludge (AGS). Filamentous algae were found to flourish at 1% salinity when nutrients were temporarily restricted to low level (COD 0, N 10 mg/L, P 0.5 mg/L). A significant improvement of granule stability was detected as the integrity coefficients of MBGS was only 0.12–0.24 rather than 0.19–0.48 of AGS under 1%-5% salinities, which reduced the risk of particle disintegration. Filamentous algae including Leptolyngbya and Geitlerinema occupied 91.2% of identified algae, and were beneficial for enhancing the biomass content and lipid production to about 1.27–1.37, 3.1–5.0 times than AGS. The MBGS had best nitrogen and phosphorus removal efficiencies of 93.4% and 64.6% at 1% salinity, and showed higher resistance to 3%-5% salinities. This study could provide meaningful information for using this modified MBGS technology in practice.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）