BIORESOURCE TECHNOLOGY
·
2016
·
IF 4.917
Hongsen Zhang,Gang Liu,Jian Zhang,Jie Bao
High titer gluconic acid and xylonic acid were simultaneously fermented by Gluconobacter oxydans DSM 2003 using corn stover feedstock after dry dilute sulfuric acid pretreatment, biodetoxification and high solids content hydrolysis. Maximum sodium gluconate and xylonate were produced at the titer of 132.46 g/L and 38.86 g/L with the overall yield of 97.12% from glucose and 90.02% from xylose, respectively. The drawbacks of filamentous fungus Aspergillus niger including weak inhibitor tolerance, large pellet formation and no xylose utilization were solved by using the bacterium strain G. oxydans . The obtained sodium gluconate/xylonate product was highly competitive as cement retarder additive to the commercial product from corn feedstock. The techno-economic analysis (TEA) based on the Aspen Plus modeling was performed and the minimum sodium gluconate/xylonate product selling price (MGSP) was calculated as $0.404/kg. This study provided a practical and economic competitive process of lignocellulose utilization for production of value-added biobased chemicals.
研究领域:
生物工程
·
可再生资源利用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Hongsen Zhang,Jian Zhang,Jie Bao
This study reported a high titer gluconic acid fermentation using dry dilute acid pretreated corn stover (DDAP) hydrolysate without detoxification. The selected fermenting strain Aspergillus niger SIIM M276 was capable of inhibitor degradation thus no detoxification on pretreated corn stover was required. Parameters of gluconic acid fermentation in corn stover hydrolysate were optimized in flasks and in fermentors to achieve 76.67 g/L gluconic acid with overall yield of 94.91%. The sodium gluconate obtained from corn stover was used as additive for extending setting time of cement mortar and similar function was obtained with starch based sodium gluconate. This study provided the first high titer gluconic acid production from lignocellulosic feedstock with potential of industrial applications.
研究领域:
生物技术
·
发酵工程
✓ 已验证
DOI
PubMed
高影响因子
Nano Research
·
2023
·Vol. 16
·期 8
·10748-10755
·
IF 9.9
Li Junhua,Jiang Yi,Zhang Xu,Huo Yidan,Du Fanglin,Tuo Yongxiao,Guo Zhiyan,Chen Dawei,Chen Shenghua
The electrooxidation of the alcohol and aldehyde molecules instead of water coupled with H 2 production has been proven to be effective for producing high-value fine chemicals under alkaline conditions. It is also noteworthy that under acidic conditions, the stability of non-noble metal water oxidation catalysts remains a great challenge due to the lattice oxygen mechanism. Hence, we coupled the biomass-derived glucose oxidation for high-value D-glucaric acid (GRA) with ultra-durable hydrogen in acid solution over a Yb-MnO 2 catalyst. The Mn 3+ regulated by Yb atoms doped in MnO 2 can effectively optimize the adsorption and desorption processes of the alcohol and aldehyde group and improve the intrinsic activity but cannot for H 2 O. The catalyst exhibited extremely high activity and stability after 50 h for glucose oxidation, inhibiting the lattice oxygen process and MnO 4− formation, while the activity was quickly lost within 0.5 h for water oxidation. Density functional theory (DFT) calculations further demonstrated that glucose oxidation reaction proceeds preferentially due to the oxidation of aldehyde group with lower adsorption-free energy (−0.4 eV) than water (Δ G > 0 eV), avoiding the lattice oxygen mechanism. This work suggests that biomass-derived glucose oxidation not only provides a cost-effective approach for high-value chemicals, but also shows an extremely potential as an alternative to acidic oxygen evolution reaction (OER) for ultradurable H 2 production.
研究领域:
生物化学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
CATALYSIS LETTERS
·
2018
·Vol. 148
·期 7
·2019-2029
·
IF 2.911
Liu Anqiu,Huang Zhong,Wang Xiaochen
Abstract A new method has been developed for the oxidation of glucose into gluconic acid over Pd/C catalysts under the room temperature and atmospheric pressure. The carbon support was prepared by the hydrothermal treatment of renewable glucose, thus contained abundant active oxygen species. The Pd/C catalyst showed high catalytic activity towards the oxidation of glucose into gluconic acid. A 100% glucose conversion and a 98% gluconic acid yield were attained within 2 h at 0.1 MPa and room temperature. Structural analysis showed that the Pd paricle sizes of the catalysts were in the range of 10.1–16.2 nm influenced by the loading of Pd. The structure/size study showed that the catalyst with optimal particle size of 10.9 nm exhibited the highest TOF (0.2388 mol glucose mol Pd /s). The catalyst showed no significant loss of activity after recycled for four times. Graphical Abstract
研究领域:
化学催化
·
绿色化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2017
·
IF 3.649
Xin Xu,Yan Li,Liping Liu,Yuhong Li,Guomei Ru,Qiannan Ding,Liping Deng,Jian Dong
Novel biocompatible and biodegradable polymers are highly desirable and crucial in drug delivery applications in order to overcome the technical challenges and problems existing in the traditional method of poly(ethylene glycol) based drug carriers. In this study, ring-opening polymerization of a carbohydrate-derived lactone , glucono-δ-lactone (GDL), generates a new highly branched polymer (PGDL) that can form stable nanoparticles through a w/o emulsification approach. The biodegradable and biocompatible particles can carry anticancer agent 5-fluorouracil (5-FU) effectively. The controlled release of 5-FU from the PGDL particles exhibits a non-Fickian mechanism without an initial burst, with an enhanced release exponent at tumoral pH. Cell viability studies by MTT assays indicate that ovarian carcinoma cells (OVCAR-3) and macrophage cells (raw 264.7) treated with PGDL (2.5 mg/ml) show no signs of toxicity in 24 h cell incubations. The PGDL particles can interact with the OVCAR-3 cell line efficiently. Therefore, the glucono-δ-lactone based particles represent an effective delivery system for cancer therapy.
研究领域:
生物医学工程
·
药剂学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2018
·
IF 2.616
Jinsha Huang,Wei Zhuang,Lei Ge,Kai Wang,Zhenfu Wang,Huanqing Niu,Jinglan Wu,Chenjie Zhu,Yong Chen,Hanjie Ying
Surface amine modification could not only improve the microenvironment near the active sites of enzyme, but also enhance the multi-point chemical crosslinking between the enzyme and carrier. The linear structured polymer of ε-poly - l - lysine (EPL) is an ideal donor of amino with much more exposed on the surface for enzyme attachment. Analysis of the result of dynamic light scattering (DLS) and circular dichroism (CD) demonstrated the favorable electrostatic interactions and negligible impact on the conformation of enzymes, Glucose Oxidase and Catalase (GOx&CAT). Titration and dissociation curve together with Zeta-potential characterization revealed that enzymes (EPL@GOx&CAT) under the protection of EPL had more stable structure and better activity and stability in acidic reaction environment. Meanwhile, the activity recovery of immobilized EPL@GOx&CAT increased to 1.56 times higher than that of GOx&CAT, and the acid resistance was improved by 1.44 times with the optimum pH shifting to acidic by 0.50 unit. The substrate affinity was raised with the decrease of K m from 5.98 to 4.35 mg mL −1 . Thus, the gluconic acid production in low pH system could be increased by pH-engineering of the enzyme microenvironment via conjugation with positively charged polyelectrolyte.
研究领域:
生物化学
·
酶工程
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2023
·
IF 7.4
Liang Zhou,Zhen Liu,Guanglong Dai,Ruxiang Qin
To address the environmentally friendly issue of fire prevention and extinguishing materials in coal mines, a new type of mine fire prevention and extinguishing gel was prepared using sodium carboxymethyl cellulose (CMC) as the matrix, ferro aluminum citrate (Fe-AlCit) as the cross-linking agent, and gluconic acid-δ-lactone (GDL) as the pH modifier. The preferred ratio was 2.5 % CMC + 4 % Fe-AlCit + 2 % GDL of No. 23 gel material by gelation time, thermal stability, strength, and permeability performance tests. The results show that the gelation time of No. 23 gel was 10 min, and its complete water loss time was 1.97 times that of water at a high temperature of 180 °C. The gel for sealing the surface of the coal body still maintained good stability after two weeks. Furthermore, A metal coordination bond favorable to the stability of gel is formed by the carboxylate ion in CMC and the double high-valent metal ions Fe 3+ and Al 3+ ionized by Fe-AlCit. No. 23 gel exhibits excellent fire prevention and extinguishing properties. The temperature-programmed test showed that the CO and C 2 H 4 gas inhibition rate of the coal sample treated with No. 23 gel reached 33.42 % and 56.86 % at 220 ℃, and the activation energy growth rate reached 26.5 % and 21.1 % in the first and second stages, respectively. The fire extinguishing test indicated that No. 23 gel can significantly reduce the temperature of the coal sample and extinguish the coal fire quickly. The study is useful to develop efficient and environmentally friendly coal mine fire prevention and extinguishing materials.
研究领域:
矿业工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Yuanjie Gu,Lin Dai,Xin Zhou,Yong Xu
The chemical compositions of lignin, hemicellulose and cellulose are so far unascertained to various lignocellulose in respect to effect of cellulose enzymatic hydrolysis . The novel and environment-friendly gluconic acid (GA) pretreatment technology showed impressive results on the enzymatic hydrolysis of cellulose in various agricultural straws. However, only few of the main reasons or critical issues pertaining to this reaction are known. Therefore, the novel GA pretreatment was carried out to remove hemicellulose from the three representative waste straws under different conditions. Next, for the enzymatic hydrolysis of the residual cellulose fraction in the pretreated straws, some mathematical correlations have been investigated between enzyme accessibility, hemicellulose removal rate, and cellulose crystallinity index. Both linear and nonlinear models were compared using five-parameter logic curve, four-parameter logic curve, and Deming regression. Hemicellulose removal was logically ascribed to be the trigger for cellulose saccharification efficiency during GA pretreatment of these waste straws.
研究领域:
生物化学
·
环境科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
YaTing Hu,Ikram ul Haq,Yong Xu,Xia Hua
Glucose-derived acids for the further production of value-added medicine, food additives, and polymers, will promote lignocellulosic biomass biorefinery industry. In response to the diversity and complexity, a new method was established by employing high performance anion exchange chromatography (HPAEC) coupled with a CarboPac™ PA200 column, for the precise and fast determination of glucose, gluconic acid, glucuronic acid, 2-ketogluconic acid, 5-ketogluconic acid and glucaric acid. Based on the analysis of tiny varieties in retention behavior, a gradient elution mode was designed and optimized for the quantitative and qualitative analysis. The protocol displayed acceptable linearity (R 2 ≥ 0.995), commendable average recovery rate (95.28% ∼ 99.89%), satisfactory precision (RSD% ≤ 1.5%), and sufficient resolution ( R > 6). Additionally, this method was successfully applied to the high-value biorefining process, which confirmed the practicability and accuracy. The results demonstrated that HPAEC has good detection performance for glucose and its derivative acids, and provide key identification technical support for the high-value utilization of lignocellulose.
研究领域:
分析化学
·
生物工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Xiangzhi Wang,Jing Wang,Zelong Li,Muhan Yang,Arbaz Rehman
Pharmaceuticals and personal care products (PPCPs), known for their severe ecological risks, are frequently detected in coastal waters worldwide. However, the environmental fate in marine systems remains poorly understood. This study investigated the co-metabolic degradation mechanism of a typical PPCP, sulfamethoxazole (SMX), by a marine consortium SAB using alginate—a natural organic matter—as a co-substrate, aiming to elucidate the biodegradation process of PPCPs in actual marine environments. An integrated approach, including metagenomic sequencing (with binning), RT-qPCR, molecular docking, metabolic modeling, and metabolomics, was employed. Results demonstrated that the SAB consortium efficiently degraded SMX at concentrations from 50 μg/L to 10 mg/L, achieving over 96% removal within 24 h. SMX degradation was achieved through metabolic cross-feeding of alginate by key species within the SAB consortium. Specifically, alginate is initially converted by Vibrio into the central metabolite 2-keto-3-deoxy-D-gluconate (KDG). KDG is subsequently metabolized by Tritonibacter , Halopseudomonas , and Vibrio , generating key metabolites such as acetyl-CoA, serine, cysteine, and guanine. These metabolites support microbial growth and induce production of SMX-degrading enzymes, such as threonine dehydratase, cysteine S-conjugate β-lyase, and guanine deaminase, leading to SMX cleavage and mineralization. Additionally, acetyl-CoA provides acetyl groups for arylamine N-acetyltransferase-mediated SMX acetylation. These processes collectively drive the co-metabolic degradation of SMX. Overall, this study provides critical insights into PPCPs' fate in marine environments.
研究领域:
环境科学
·
海洋生物学
·
微生物学
·
药物污染
·
生物降解
·
代谢工程
·
宏基因组学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Zhen Zou,Dinggeng He,Linli Cai,Xiaoxiao He,Kemin Wang,Xue Yang,Liling Li,Siqi Li,Xiaoya Su
The outstanding progress of nanoparticles-based delivery systems capable of releasing hypoglycemic drugs in response to glucose has dramatically changed the outlook of diabetes management. However, the developed glucose-responsive systems have not offered real-time monitoring capabilities for accurate quantifying hypoglycemic drugs released. In this study, we present a multifunctional delivery system that integrates both delivery and monitoring issues using glucose-triggered competitive binding scheme on alizarin complexone (ALC) functionalized mesoporous silica nanoparticles (MSN). In this system, ALC is modified on the surface of MSN as the signal reporter. Gluconated insulin (G-Ins) is then introduced onto MSN-ALC via benzene-1,4-diboronic acid (BA) mediated esterification reaction, where G-Ins not only blocks drugs inside the mesopores but also works as a hypoglycemic drug. In the absence of glucose, the sandwich-type boronate ester structure formed by BA binding to the diols of ALC and G-Ins remains intact, resulting in an fluorescence emission peak at 570 nm and blockage of pores. Following a competitive binding, the presence of glucose cause the dissociation of boronate ester between ALC and BA, which lead to the pores opening and disappearance of fluorescence. As proof of concept, rosiglitazone maleate (RSM), an insulin-sensitizing agent, was doped into the MSN to form a multifunctional MSN (RSM@MSN-ALC-BA-Ins), integrating with double-drugs loading, glucose-responsive performance, and real-time monitoring capability. It has been demonstrated that the glucose-responsive release behaviors of insulin and RSM in buffer or in human serum can be quantified in real-time through evaluating the changes of fluorescence signal. We believe that this developed multifunctional system can shed light on the invention of a new generation of smart nanoformulations for optical diagnosis, individualized treatment, and noninvasive monitoring of diabetes management.
研究领域:
纳米技术
·
药物递送系统
·
生物医学工程
·
糖尿病研究
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Energy
·
2025
·
IF 8.6
Zhenhe Chang,Wei Zhang,Rui Mu,Chaoyue Zhang,Quanwei Shi,Baohui Wang,Hongjun Wu,Di Gu
Aqueous zinc-ion batteries (AZIBs) are promising candidates for grid-scale energy storage owing to their inherent safety, low cost, and high theoretical capacity (820 mAh/g). However, membrane-free configurations suffer from severe lifespan limitations caused by interfacial side reactions and zinc dendrite growth. In this work, we pioneer a bimolecular synergistic electrolyte strategy based on sorbitol-gluconate complexes regulated by van der Waals interactions. The coordinated molecules simultaneously modulate zinc deposition morphology via anode surface adsorption and reconstruct the electrolyte solvation structure through hydrogen bonding, effectively suppressing dendrite formation, dead zinc accumulation, and parasitic hydrogen evolution. The optimized electrolyte enables Zn//Zn cells to achieve exceptional cycling stability (585 h at 10 mA/cm 2 ) 6-fold longer than baseline systems. When deployed in membrane-free Zn-Br 2 batteries, this synergy-driven chemistry demonstrates unprecedented compatibility with separator-less architectures, enabling more than 4000 stable cycles (1600 h) at 10 mA/cm 2 with 96.2 % Coulombic efficiency–tripling the lifespan of conventional ZnBr 2 electrolytes. Operando characterization confirms the dynamic self-adaptation of van der Waals-bonded complexes during cycling, which maintains stable electrode-electrolyte interfaces. This emergent bimolecular synergy redefines the design rules for high-performance, low-cost zinc batteries that operate without membranes.
研究领域:
储能技术
·
电化学
·
材料科学
·
电池技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）