FOOD SCIENCE AND BIOTECHNOLOGY
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2022
·Vol. 32
·期 2
·145-156
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IF 3.231
Zhu Lei,Wu Xin,Hu Xixi,Li Xinyue,Lv Shanshan,Zhan Chuan,Chen Yunhua,Wang Changyuan,Xu Jingyu
The total contents and antioxidant activities of phenolic compounds as well as anthocyanin profiles were analyzed and compared in fresh berries and fermented pomace of three grape cultivars with different pedigrees. The phenolic contents and antioxidant activities decreased significantly in skins ( p < 0.05), while relatively large amounts of them were retained in seeds after fermentative maceration. Fermentative maceration also had a significant impact on the anthocyanin compositions. The proportions of anthocyanins with more stable structures, such as malvidin derivatives, methylated, diglucosides and nonacylated anthocyanins, increased significantly in the pomace skins ( p < 0.05). There were obvious differences in phenolic features and anthocyanin profiles among the three cultivars. ‘NW196’, a wine hybrid of Vitis vinifera and V. quinquangularis , was characterized by the highest total anthocyanin contents and degree of diglucosylation. The results obtained in this study could contribute to the primary data for the development and utilization of winemaking pomace, especially from local non- Vitis vinifera grapes.
研究领域:
农业
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食品科学
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化学
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DOI
PubMed
高影响因子
Journal of Nano Research
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2016
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IF 0.366
Qing Quan Guo,Xin Fu Ma,Hai Xiang Ma,Yao Lu
The preparation of size- and shape- controlled metallic nanoparticles using biological methodologies is a noticeably stimulating research field due to their unique physicochemical properties. In this paper, biosynthesis of anisotropic AuNPs using Ampelopsis grossedentata extract and the effects of halide ions on the formation of AuNPs has been demonstrated. The sizes and morphologies of AuNPs were characterized by UV-vis-NIR spectrophotometer and Transmission Electron Microscopy (TEM). It showed that the shape, size and optical properties of AuNPs can be fine-tuned by varying the dosage of the vine tea extract. The presence of halogen ions has significantly influence the morphology of AuNPs during the synthesis process. Both of Br- and Cl- could produced nanoplates, whereas I- distorted the triangle nanoparticles to induce the formation of aggregated spherical ones.
研究领域:
纳米技术
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材料科学
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生物技术
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化学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2013
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IF 2.487
Shian Zhong,Yanyue Kong,Ling Zhou,Chengyun Zhou,Xiaona Zhang,Yan Wang
In this study, we developed an efficient conversion process of dihydromyricetin to myricetin from Ampelopsis grossedentata extracts. The content of myricetin increased from 2.38% to 85.57%, demonstrating the successful dehydrogenation of dihydromyricetin. Molecularly imprinted polymers (MIPs) were prepared by surface imprinting method using silica microspheres as the support matrices and myricetin as template. The MIPs were applied for the selective adsorption of myricetin. The chemical structure of the MIPs was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. Static, dynamic and selective adsorption experiments showed that the MIPs exhibited good adsorption ability, rather fast template rebinding kinetics, and appreciate selectivity over structurally related compounds. Accordingly, the MIPs were applied as the selective sorbent in SPE to purify myricetin obtained through dehydrogenation, followed by HPLC-UV analysis. The recoveries of myricetin and dihydromyricetin were 92.7% and 55.6%, respectively. This study demonstrates the feasibility of using the developed MIP-SPE method to purify and enrich myricetin in the natural products.
研究领域:
药学
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分析化学
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天然产物化学
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DOI
PubMed
高影响因子
Agriculture-Basel
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2022
·Vol. 12
·期 5
·604
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IF 3.408
Xing Han,Fei Yao,Ying Wang,Xinyao Duan,Zhilei Wang,Yihan Li,Tingting Xue,Xu Liu,Hua Wang,Hua Li
Biodegradable liquid film (BLF) improves soil structure and increases plant freezing tolerance after spraying on the surface of soil and plant. In this study, the effects of BLF on grape composition and volatile compounds in Cabernet sauvignon (Vitis viniferaL.) grapes were determined by spraying BLF during the dormant periods over three years. The aim of the study was to evaluate the potential impact of BLF as an overwintering protection measure on grape fruit quality. In 2020 and 2021, BLF spraying increased reducing sugar content and 100-berry weight, decreased titratable acid content, and improved the maturity factor. Compared with the vines not sprayed with BLF, the content of total phenols and total anthocyanins in grape skins showed an increase over the three-year period, with the largest increases of 31.92% and 48.38%, respectively, and the content of total tannins and total flavan-3-ols increased in 2020 and 2021. BLF treatment also increased the total phenolic content in seeds for all three years, reaching a significant level in 2019, 16.38% higher than control treatment (CK). HPLC analysis showed that BLF treatment affected the content and composition of monomeric anthocyanins in grape skins, especially in 2021, BLF treatment significantly increased the content of nine monomeric anthocyanins, and the proportion of acetylated and coumaroylated anthocyanins. However, GC-MS analysis indicated that BLF had little effect on volatile compounds. These results suggest that BLF can be used as an overwintering protection measure in cold regions to promote the accumulation of sugars and polyphenolics, thereby improving overall grape quality.Keywords:wine grape;biodegradable liquid film;grape composition;anthocyanins
研究领域:
农业
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酿酒学
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植物科学
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Haihui Pan,Wenjuan Jiao,Feilin Wang,Yuanhao Sheng,Yousheng Zhang,Fansheng Kong
A novel strategy was devised to synthesize robust nanocarriers by conjugating fish collagen peptides (FCP) with Ampelopsis grossedentata polysaccharide (AGP), culminating in the creation of FCP-AGP nanoparticles (FCAGPs) for advanced drug delivery systems. The innovative approach entailed the systematic investigation of particle size, zeta potential, and surface hydrophobicity alterations in FCAGPs upon varying AGP concentrations. The optimized FCAGP-4 nanoparticles, boasting a precisely engineered AGP content of 2.0 % (w/v) and an average particle diameter finely tuned to 353.2 ± 10.5 nm, demonstrated outstanding characteristics in dispersibility, stability, and emulsifying capacity. Furthermore, the water contact angle of FCAGP nanoparticles notably increased from 46.3 ± 2.8° to 70.2 ± 5.6°, indicating enhanced surface hydrophobicity. The mechanism by which FCAGP nanoparticles were attached to the surface of oil droplets to stabilize the emulsions was elucidated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Significantly, a modified sample pretreatment technique was employed to demonstrate clearly the emulsion phase interface on the SEM.Innovative FCAGP-stabilized Pickering emulsions achieved 65.39 % in DMY bioavailability, facilitating its slow-release and enhanced bioaccessibility during in vitro digestion. Pickering emulsions loaded DMY demonstrated improved stability and sustained release under simulated digestive conditions, bolstering its therapeutic efficacy. Our findings solidify the theoretical basis for green synthesis of propeptide-polysaccharide nanoparticles and introduce a novel strategy for targeted propeptide Pickering emulsions in drug delivery, highlighting the transformative potential of FCAGP nanoparticles in enhancing lipophilic drug bioavailability and pharmacological impact.
研究领域:
纳米技术
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药剂学
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药物递送系统
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材料科学
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生物物理学
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胶体科学
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DOI
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 9
·2100892
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IF 6.575
Ying Li,Haiming Hu,Huabing Yang,Aizhen Lin,Hui Xia,Xue Cheng,Mingwang Kong,Hongtao Liu
Scope Vine tea ( Ampelopsis grossedentata ), a traditional Chinese tea, has displayed various biological activities. The authors aim to investigate the effect of Vine Tea ( Ampelopsis grossedentata ) extract (VTE) on carbon tetrachlorid (CCl 4 )induced acute liver injury (ALI) in mice and to explore the underlying role of gut microbiota during the treatment. Methods and Results C57BL/6J mice injected with CCl 4 are treated with VTE for 6 weeks. By using H&E staining, immunofluorescence staining, quantitative real-time (qRT)-PCR, and western blot, it is shown that VTE treatment significantly ameliorates hepatocyte necrosis, alleviates the mRNA levels of toll-like receptor 4 ( Tlr4) , interleukin (Il)-6 , inducible nitric oxide synthase (iNOS) , acetyl-CoA carboxylase 1 (Acc1) , and increases the mRNA levels of peroxisome proliferator-activated receptor gamma (Ppar-γ) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (Hmg-coar) compared to the CCl 4 group. Also, VTE abrogates the decreased mRNA expressions of zonula occludens-1 (Zo-1) , Occludin , and Mucin1 in colon tissues. Using microbial 16S rDNA sequencing, VTE treatment significantly downregulates the abundances of some harmful intestinal bacteria like Helicobacter and Oscillibacter . In contrast, VTE upregulates the contents of several beneficial bacteria, such as Ruminococcaceae_UCG-014 and Eubacterium_fissicatena_group . Further, VTE fails to improve ALI in the mice with gut microbiota depletion using antibiotic treatment. Conclusions The studies suggest that VTE exhibits a protective effect against CCl 4 -induced ALI in mice by alleviating hepatic inflammation, suppressing intestinal epithelial barrier injury, and restoring gut microbiota dysbiosis.
研究领域:
药理学
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微生物学
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肝病学
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免疫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
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