FOOD HYDROCOLLOIDS
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2014
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IF 4.28
Ping Shao,Jiafeng Zhang,Zhongxian Fang,Peilong Sun
Chlorogenic acid (CGA) is a polyphenol commonly existed in fruits and vegetables with a variety of bioactivities. To increase its stability and potential broader industrial application, CGA/cyclodextrin (CD) complexes were prepared using β-cyclodextrin (β-CD) and its derivative (2-hydroxypropyl)-β-cyclodextrin (2-HP-β-CD). Their inclusion complexation behavior and characterization were investigated by FT-IR, XRD, SEM, and 1 H NMR spectroscopy. The morphologic and spectral studies indicated the formation of inclusion complexes. Moreover, spatial arrangements of inclusion complexes were proposed based on 1 H NMR results. The antioxidant activities of the complexes were improved compared with the CGA without inclusion, and the CGA/HP-β-CD complexes showed the highest antioxidant activity. In addition, the CGA/CD complexes were added in grape juice to explore the potential on the preservation of anthocyanin and color quality. Results showed that the degradation of anthocyanins was reduced when CGA and CGA/CDs inclusions were added in grape juice due to copigmentation effect, and their copigmentation strength showed in an order of CGA/HP-β-CD complex > CGA/β-CD complex > free CGA.
研究领域:
食品科学
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化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
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2017
·Vol. 42
·期 1
·e13344
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IF 0.791
Xu Tang,Yun Wang,Juan Han,Lei Wang,Cheng Li,Liang Ni
Liquid liquid extraction, as a clean and green process, has a great potential in technology and innovation, which has been widely used in industrial production. In this paper, a liquid–liquid extraction process based on L44-NaH 2 PO 4 ATPS was studied for separation and purification of anthocyanin and vitis linn polysaccharide from the grape juice. The concentrations of L44 and NaH 2 PO 4 , temperature and pH as factors of effect on the extraction rates were discussed. Under the optimal conditions, the extraction rates of anthocyanin and vitis linn polysaccharides were 99.8% and 90.3%, respectively. The work subject is extraction of two compounds from grape juice, which can be very important for food, pharmaceutical and cosmetic industries. The vitis linn polysaccharides were further purified by dialysis and the impurity protein was successfully removed based on organic solvent precipitation method. Besides, the recycle of L44 was also achieved through secondary extraction. Practical applications A liquid–liquid extraction process based on L44-NaH 2 PO 4 ATPS was studied for separation and purification of anthocyanin and vitis linn polysaccharide from the grape juice. The efficient separation of two kinds of water-soluble components is of great significance in industrial production. It is very interesting for scientific communities and shows a great biotechnological potential of the ATPS purification process. The work subject is extraction of two compounds from grape juice, which can be very important for food, pharmaceutical and cosmetic industries.
研究领域:
食品科学
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生物技术
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2014
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IF 3.065
Yingchun Wu,Yun Wang,Wenli Zhang,Juan Han,Yan Liu,Yutao Hu,Liang Ni
Aqueous two-phase extraction (ATPE) was employed for the extraction and preliminary purification of anthocyanins in grape juice (GA). The influences of such factors as the type and concentration of salt, the concentration of alcohol and the quantity of grape juice, extraction time, system pH and temperature were investigated to obtain the optimal extraction conditions. They were listed as follow: sodium dihydrogen phosphate (NaH 2 PO 4 ) concentration 28% (w/w), ethanol concentration 25% (w/w), the quantity of grape juice 1 mL, temperature 298.15 K, time 1 h and no pH adjustment. Under the optimal conditions, the relative and absolute recovery of GA in the top phase reached 99.35% and 99.26% in one step, respectively, while the sugar-removing rate reached 75.08%. The results of Multistage and scale-up extractions showed that it could remain over 90% GA in the top phase and remove more than 90% sugars after continuous extraction for two times. Scale-up extractions and the GA stability results showed the effectiveness and great potential of this method in processing mass grape juice.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2018
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IF 3.129
Jianfei He,Fengxian Guo,Luan Lin,Hongbin Chen,Jie Chen,Yongqiang Cheng,Zong-Ping Zheng
The inclusion complexes of oxyresveratrol (Oxy) with β-cyclodextrin (β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) were prepared and characterized by UV–vis spectroscopy, FT-IR spectroscopy, XRD , TG-DTG and SEM . Based on the optimal formulation, 222.23 ± 1.52 μg and 232.65 ± 2.64 μg of Oxy was included in 1.00 mg of the Oxy-β-CD and Oxy-HP-β-CD dried powder with the water solubility up to 14.44 ± 0.83 mg/mL and 47.33 ± 0.78 mg/mL, yielding about 30 and 100-fold increase in Oxy solubility (about 0.47 mg/mL), respectively. The physical stability and dissolution rate study showed that the formation of inclusion complexes significant enhanced the storage stability of Oxy and its dissolution rate. These results indicated that β-CD and HP-β-CD may be suitable delivery systems of Oxy. Furthermore, as illustrated in this study, inclusion complexes exhibited strong antibrowning effects on fresh grape juice in 24 h, showing its great potential application in the food industry.
研究领域:
食品科学
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药物化学
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分析化学
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DOI
高影响因子
FOOD CHEMISTRY
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2023
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IF 8.8
Kunfeng Liu,Yahui Guo,Hang Yu,Yuliang Cheng,Yunfei Xie,Weirong Yao
In this study, sulfydryl-functionalized nitrogen-doped carbon dots (SH-NCDs) was synthesized by amide reaction of hydrothermally synthesized carbon dots with l -cysteine and used to detect patulin selectively. The SH-NCDs exhibited excitation wavelength-independent fluorescence in the range 300–360 nm. The modified sulfhydryl group (-SH) on the surface of NCDs served as a specific recognition site to capture patulin. The addition reaction between patulin and the -SH on the SH-NCDs surface resulted in enhanced fluorescence. SH-NCDs was used as a fluorescent probe for label-free detection of patulin, showing excellent sensitivity in the linear range of 0.1–400 ng mL −1 , with detection limits as low as 0.053 ng mL −1 . The fluorescent probe has specific selectivity for patulin. The recoveries of patulin in apple juice and grape juice were 88.9 %–99.2 % and 92.5 %–101.8 %, respectively. These results showed that the sensor designed in this experiment selectively detected the target patulin from complex food systems.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xinqi Long,Qi Wu,Lu Yang,Longyingzi Xie,Lanrui Ma,Qiyang Zhao,Yongliang Cui,Yue He,Yaohai Zhang
Aptasensors for detection of ochratoxin A (OTA) have been extensively studied, but the majority of them require costly and large-scale equipment as signal readers. Herein, a photothermal aptasensor capable of portable detection of OTA through a thermometer was developed on basis of aptamer structural switching and rolling circle amplification (RCA)-enriched DNAzyme. Oligonucleotides and alkaline phosphatase (ALP) modified magnetic beads were prepared. The binding of aptamers to OTA led to the release of ALP labeled complementary DNA. After magnetic separation, ALP catalyzed the padlock dephosphorylation, inhibiting the subsequent RCA reaction. This process converted the OTA concentration into the amount of the photothermal reagent oxTMB produced from the catalytic reaction induced by RCA-enriched DNAzyme. Under the optimal conditions, the detection limit (LOD) of this aptasensor was 2.28 nM in a clean buffer, while the LOD reached 2.43 nM in 2 % grape juice. The good performance of the photothermal aptasensor makes it possible to measure OTA pollution in low resource environments.
研究领域:
分析化学
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生物传感器
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食品安全
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
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2017
·Vol. 10
·期 10
·3434-3442
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IF 2.038
Hu Kai,Deng Zhifen,Li Sui,Wu Mingxia,Liu Wei,Zhang Shusheng
In this study, bis(tetraoxacalix[2]arene[2]triazine) modified silica gel was successfully prepared and used as an efficient sorbent for solid-phase extraction. Coupled with ultra-high pressure liquid chromatography (UHPLC), the extraction performance of the sorbent was evaluated by using three flavonoids as model analytes. Main parameters, which affecting extraction efficiency were carefully optimized. The results showed that multiple intermolecular interactions were involved in the sample pretreatment procedure, including π–π, hydrophobic and hydrogen bonding interactions. Under the optimal conditions, the proposed method was applied for the analysis of three flavonoids in grape juice. Satisfactory linear ranges for flavonoids were obtained in the range of 5–200 ng mL −1 for quercetin, 1–200 ng mL −1 for luteolin, and 2–200 ng mL −1 for kaempferol, with good correlation coefficients (>0.9996). Limits of detection (LODs) were in the range of 0.5–2 ng mL −1 , and the LOQs were between 1 and 5 ng mL −1 . The recovery values of spiked grape juice ranged from 97 to 106% with relative standard deviations (RSDs) less than 4.7% ( n = 3). This method exhibited the advantages of simplicity, rapidity, and low solvent consumption, and was promising for the separation and determination of flavonoids in grape juice and other matrixes.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Mengya Zhao,Hong Ren,Zhuomin Yan,Jing Ma,Xiaoping Feng,Di Liu,Fangyu Long
Ochratoxin A (OTA) contamination in grape juice has attracted widespread concern as OTA can lead to kidney disease and cause adverse neurological effects. An effective method to remove OTA is to make use of highly adsorbent materials that are able to remove the toxic contaminant. Recently, inactivated Lactobacillus plantarum- based biosorbents have shown to be an efficient, cost-effective and environmentally friendly bioremediation method in removing toxic pollutants such as OTA. We used five chemical thiol-modification methods to improve the adsorption efficiency of OTA in grape juice. The esterification of Lactobacillus plantarum (L-Es) significantly increased the sulfhydryl contents (-SH) by 251.33 μmol/g and >90% of OTA was removed. However, the inactivated microbial adsorbent was difficult to separate after adsorption and therefore, the prepared L-Es were embedded into the cellulose nanocrystals (L-Es@CNCs). Moreover, L-Es@CNCs significantly increased the adsorption rate of OTA in grape juice samples by 88.28% with negligible effects on juice quality due to the properties of easy re-use and excellent biodegradability. This showcases its potential application for OTA removal in the grape juice industry.
研究领域:
食品科学
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生物技术
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Kunxia Li,Xiujuan Qiao,Haiyan Zhao,Yaping He,Qinglin Sheng,Tianli Yue
In this work, we have synthesized the novel carbon dots-black phosphorus nanohybrid (CDs-BP). And a novel label-free electrochemical aptasensor based on CDs-BP was constructed for ultrasensitive detection of Ochratoxins A (OTA). The morphology and structure of nanocomposite were analyzed by transmission electron microscope, X-ray photoelectron spectroscopy, Zeta potential, et al. The CDs-BP nanohybrid, an excellent carrier for the immobilization of aptamer (Apta), was prepared through C-P bond, resulting in the enhancement of the electrochemical sensing performance for OTA detection. The results showed that the aptasensor had a wide linear range towards OTA (0.1 fg/mL − 10.0 ng/mL) with a low detection limit of 0.03 fg/mL under the optimal conditions. The proposed aptasensor displayed good electrochemical stability and excellent selectivity to OTA detection. Moreover, a satisfactory practical application results were obtained for different actual samples of wheat and grape juice samples. Thus, a new aptasensor was raised based on the CDs-BP nanocompound for the electrochemical detection of contaminants in edible agricultural food.
研究领域:
分析化学
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纳米技术
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食品安全
·
电化学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Xiaolan Wang,Tao Feng,Juntao Wang,Lin Hao,Chun Wang,Qiuhua Wu,Zhi Wang
A magnetic porous covalent triazine-based organic polymer (M-PCTP) was synthesized by co-precipitation method. The M-PCTP combined both the properties of the PCTP and the magnetism of Fe 3 O 4 nanoparticles, possessing highly porous structure and good magnetism. It was used as a magnetic solid phase extraction (MSPE) adsorbent to extract carbamate pesticides (propoxur, carbaryl, isoprocarb, fenobcarb and diethofencarb) from lemonade and grape juice samples prior to high performance liquid chromatography-mass spectrometric detection. Under the optimal conditions, a good linearity for the real samples was received in the range from 0.10–0.90 to 80.00 ng mL −1 with the correlation coefficients of 0.9882–0.9983. The method recoveries for the five carbamates were 86.3–108.0%. The limits of detection were 0.02–0.20 ng mL −1 for lemonade sample, and 0.04-0.30 ng mL −1 for grape juice sample. The M-PCTP also demonstrated good extraction capabilities for other different kinds of organic compounds including chlorophenols and polycyclic aromatic hydrocarbons.
研究领域:
分析化学
·
环境科学
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材料科学
·
食品科学
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DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Zou Yanqi,Zhou Xiaoyu,Xie Liuhong,Tang Hongliang,Yan Fei
Carbendazim (CBZ), a kind of widely used pesticide, is harmful to human health and environmental ecology. Therefore, it is of great importance to detect CBZ in real samples. Herein we report the stable growth of vertically-ordered mesoporous silica films (VMSF) on the glassy carbon electrode (GCE) using boron nitride-reduced graphene oxide (BN-rGO) nanocomposite as an adhesive and electroactive layer. Oxygen-containing groups of rGO and 2D planar structure of BN-rGO hybrid favor the stable growth of VMSF via the electrochemically assisted self-assembly (EASA) method. Combining the good electrocatalytic activity of BN-rGO and the enrichment effect of VMSF, the proposed VMSF/BN-rGO/GCE can detect CBZ with high sensitivity (3.70 μA/μM), a wide linear range (5 nM–7 μM) and a low limit of detection (2 nM). Furthermore, due to the inherent anti-fouling and anti-interference capacity of VMSF, direct and rapid electrochemical analyses of CBZ in pond water and grape juice samples are also achieved without the use of complicated sample treatment processes.
研究领域:
分析化学
·
环境科学
·
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）