FOOD CHEMISTRY
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2018
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IF 4.946
Lanxin Luo,Yan Cui,Jinhui Cheng,Bairui Fang,Zongmin Wei,Baoshan Sun
To develop an efficient method for degradation of grape seed and skin proanthocyanidins polymers into oligomers, an optimized sulphurous acid degradation conditions for grape seed with the temperature of 60 °C, reaction time of 60 min and sample-sulphurous acid ratio of 1:0.2, and for grape skin with the temperature of 40 °C, reaction time of 60 min and sample-sulphurous acid ratio of 1:0.2, were established. Afterwards, HSCCC and prep-HPLC were used to fractionate and isolate individual proanthocyanidin oligomers from the degradation products. Total of ten dimeric or trimeric procyanidins were obtained, and most of them presented high yield (from 0.7 mg to 13.6 mg per run in grape seed and from 0.5 mg to 4.1 mg per run in grape skin) and high purity (over 90%). The proposed method provides a new way for large preparation of oligomeric proanthocyanidins from naturally abundant and wasted polymeric ones.
研究领域:
植物化学
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食品科学
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天然产物化学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
·
IF 7.129
Danyu Zhao,Huilan Yi,Nan Sang
Arsenic (As) is known to induce toxic responses in many organs of human beings and animals. However, research concerning toxicity in the stomach is limited. In this study, arsenic-induced gastric toxicity was investigated in a mouse model, and grape skin extract (GSE) was confirmed to have protective effects against arsenic toxicity. Our experimental results showed that exposure to 10 mg/l arsenic via drinking water for 56 days caused oxidative damage and inflammatory responses. The H 2 O 2 and malondialdehyde (MDA) contents were significantly increased, accompanied by significant decreases in total superoxide dismutase (T-SOD) activity and glutathione (GSH) content in the gastric tissue of arsenic-treated mice. Two inflammatory signalling pathways, i.e., TLR2/MyD88/NF-κB and IL-6/STAT-3, were activated, along with inflammatory cell infiltration and the elevated mRNA expression of pro-inflammatory cytokines ( TNF-α, IL-1β and IFN-γ ) and myeloperoxidase (MPO) in the gastric tissue of mice exposed to arsenic. Meanwhile, the mRNA levels of the ZO-1 , ZO-2 and occludin genes, which encode the key components of tight junction (TJ) complexes, were downregulated. However, the application of GSE (300 mg/kg bw) significantly inhibited the arsenic-induced increases in H 2 O 2 and MDA contents and the decreases in T-SOD activity and GSH content. The arsenic-mediated gene expression of pro-inflammatory cytokines (TNF-α, IL-1β and IFN-γ), MPO and IL-6/STAT3 and TLR2/MyD88/NF-κB pathways was found down-regulated. Moreover, the arsenic-induced inflammatory cell infiltration and inhibition of TJ genes transcription were markedly attenuated in the As+GSE (300 mg/kg bw) group. Based on the present findings, arsenic intake appears to cause gastric toxicity via oxidative stress and inflammation, and the application of GSE offers significant protection against arsenic toxicity in a mouse model by attenuating the oxidative stress and inflammatory response. Our results suggest that GSE by oral administration might function as a candidate therapeutic supplement to antagonize arsenic toxicity.
研究领域:
毒理学
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营养学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
International Journal of Environmental Research and Public Health
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2018
·Vol. 15
·期 2
·239
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IF 2.145
Lin Ma,Chunhai Jiang,Zhenyu Lin,Zhimin Zou
Biosorption using agricultural wastes has been proven as a low cost and efficient way for wastewater treatment. Herein, grape peel treated by microwave- and conventional-hydrothermal processes was used as low cost biosorbent to remove methylene blue (MB) from aqueous solutions. The adsorption parameters including the initial pH value, dosage of biosorbents, contact time, and initial MB concentration were investigated to find the optimum adsorption conditions. The biosorbent obtained by microwave-hydrothermal treatment only for 3 min at 180 °C (microwave-hydrothermal treated grape peel, MGP) showed faster kinetics and higher adsorption capability than that produced by a conventional-hydrothermal process (hydrothermal treated grape peel, HGP) with a duration time of 16 h. The maximum adsorption capability of MGP under the optimum conditions (pH = 11, a dosage of 2.50 g/L) as determined with the Langmuir model reached 215.7 mg/g, which was among the best values achieved so far on biosorbents. These results demonstrated that the grape peel treated by a quick microwave-hydrothermal process can be a very promising low cost and efficient biosorbent for organic dye removal from aqueous solutions.Keywords:grape peel;biosorbent;biosorption;methylene blue;wastewater treatment
研究领域:
环境科学
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生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2016
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IF 4.052
Lanxin Luo,Yan Cui,Shuting Zhang,Lingxi Li,Yuanyuan Li,Peiyu Zhou,Baoshan Sun
To develop an efficient method for large preparation of various individual polyphenols from white grape skins (Fernão Pires; Vitis vinifera ) by preparative high-speed counter-current chromatography (HSCCC) and preparative-HPLC, an optimized preparative HSCCC condition with two-phase solvent system composed of Hex-EtOAc-H 2 O (1:50:50, v/v) was used to separate grape skin polyphenols into various fractions. Both the tail-head and head-tail elution modes were used with a flow rate of 3.0 ml/min and a rotary speed of 950 rpm. Afterwards, a preparative-HPLC separation was applied to isolate individual polyphenols in each of the fractions from HSCCC. Total of 7 fractions (Fraction A to G) were obtained from grape skin extract by HSCCC. After preparative-HPLC isolation, fifteen individual compounds were obtained, most of which presented high yields and purity (all over 90%). The HSCCC method followed with preparative-HPLC appeared to be convenient and economical, constituting an efficient strategy for the isolation of grape skin polyphenols.
研究领域:
食品科学
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植物化学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
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IF 3.3
Wenyu Cui,Fuchun Chen,Zhaoyue Sun,Caifang Cui,Ben Xu,Weijun Shen,Fachun Wan,Anwei Cheng
BACKGROUND Grape peels, the main by-products of wine processing, are rich in bioactive ingredients of phenolics, including proanthocyanidins, flavonoids and anthocyanins. Phenolics have the function of regulating intestinal microbiota and promoting intestinal health. From the perspective of the dietary nutrition of grape peel phenolics (GPP), the present study aimed to investigate the influence of GPP on the composition and metabolism of human gut microbiota during in vitro fermentation. RESULTS The results indicated that GPP could decrease pH and promote the production of short-chain fatty acids. ACE and Chao1 indices in GPP group were lower than that of the Blank group. GPP enhanced the levels of Lachnospiraceae UCG-004 , Bacteroidetes and Roseburia , but reduced the Firmicutes / Bacteroidetes ratio. Kyoto Encyclopedia of Proteins and Genome enrichment pathways related to phenolic acid metabolism mainly included flavonoid, anthocyanin, flavone and flavonol biosynthesis. Gut microbiota could accelerate the release and breakdown of phenolic compounds, resulting in a decrease in the content of hesperetin-7- O -glucoside, delphinidin-3- O -glucoside and cyanidin-3-rutinoside etc. In vitro antibacterial test found that GPP increased the diameters of the inhibition zones of Escherichia coli and Staphylococcus aureus in a dose-dependent manner. CONCLUSION The results of the present study revealed that GPP might be a potential prebiotic-like to prevent diseases by improving gut health. The findings could provide a theoretical basis for the potential to exploit GPP as dietary nutrition to maintain intestinal function. © 2024 Society of Chemical Industry.
研究领域:
食品科学
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微生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
POLYMER ENGINEERING AND SCIENCE
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2024
·Vol. 64
·期 8
·3431-3443
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IF 3.2
Shihui Wang,Biao Zhao,Yanling Hao,Qixiang He
In this study, the optimal ratio of starch and polyvinyl alcohol (PVA) was successfully selected by response surface method. The novel packaging films were fabricated by immobilizing Kyoho skin extract (KSE) within starch/PVA composite film by the casting method. The mechanical property, PH sensitivity, and other properties of films with different KSE content (1–5 mL) were studied. The starch/PVA film with 3 mL KSE (SPK3.0 film) showed more uniform structure than the starch/PVA film in microscope. At the same time, the film has strong mechanical properties and low water barrier capacity. Infrared analysis of the film further revealed hydrogen bond interaction between film matrix and KSE. The smart packaging film displayed distinguishable changes of color from purple to green in pH (1–13). Furthermore, the smart films possessed excellent antioxidant. Therefore, the SPK films had the potential application potential in the field of packaging. Highlights Barrier properties and antioxidant activity were enhanced significantly by the addition of KSE. The film components are connected by hydrogen bonds so that they have good compatibility. The functional film shows different colors in the range of pH 1–13.
研究领域:
食品科学
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材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Cheng Pan,Xiaofei Li,Yinao Jiao,Guozhi Fan,Yifei Long,Qunpeng Cheng,Haitao Yang
To enhance the mechanics performance, sensitivity and response range of multi-responsive photonic films, herein, a facile method for fabricating multi-responsive films is demonstrated using the evaporative self-assembly of a mixture of grape skin red (GSR), cellulose nanocrystal (CNC), polyvinyl alcohol (PVA) and deep eutectic solvent (DES). The prepared materials exhibited excellent thermal stability , strain properties, solvent resistance , ultraviolet (UV) resistance and antioxidant activity . Compared to a pure PVA film, the presence of GSR strengthened the antioxidant property of the film by 240.1 % and provided excellent UV barrier capability. The additional cross-linking of DES and CNC promoted more efficient phase fusion, yielding a film strain of 41.5 %. The addition of hydrophilic compound GSR, wetting and swelling due to the DES and the surface inhomogeneity of the films rendered the multi-responsive films high sensitivity, wide response range and multi-cyclic stability in environments with varying pH and humidity. A sample application showed that a PVA/CNC/DES film has the potential to differentiate between fresh, sub-fresh and fully spoiled shrimps . The above results help in designing intelligent thin film materials that integrate antioxidant properties, which help in monitoring the changes in food freshness and food packaging.
研究领域:
食品科学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
CyTA-Journal of Food
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2021
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IF 2.255
Wenjuan Lou,Bo Li,Grevtseva Nataliya
Grape seed powder (GSP), grape skin powder (GSKP), or grape pomace powder (GPP) was introduced as partial substitution for wheat flour (WF) with variable percentages by weight (0– 20 wt%), and the resultant addition effects on the rheological and microstructural properties were studied. GPP and GSP significantly (p < .05) reduced the water absorption but three types of grape powders could increase the development time and dough stability. Furthermore, all kinds of grape powders with various incorporations could significantly (p < .05) enhance the strength of dough systems. Grape powders decreased the storage (G′) and loss (G″) moduli, while the tan δ increased with the increasing of GSP/GSKP/GPP addition levels. The microstructure observation of composite doughs suggested that the integrity and continuity of gluten networks were destroyed in the presence of grape powders. Based on the above results, the GSP addition levels of 10% to 15%, GSKP/GPP of 5% to 10% are recommended.
研究领域:
食品科学
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植物化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Shuhan You,Xiaoyun Zhang,Yilin Wang,Yaobo Jin,Manman Wei,Xiudan Wang
Color indicator films for fish freshness were fabricated by incorporating κ-carrageenan (CAR) polymer with red grape skin extract (GSE) as a pH-sensing agent and silver nanoparticles (AgNPs) as an antimicrobial agent. Anthocyanins in GSE exhibited distinguished pH responsive color changes. GSE and AgNPs were well compatible with CAR with intramolecular interactions, approved by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis, thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC). GSE-containing films displayed distinguished color changes in response to pH variations and volatile ammonia. Enhanced UV blocking ability and strong antioxidant activity were revealed for GSE included films without sacrificing the physico-chemical properties of the CAR film. Films containing AgNPs showed improved mechanical strength and strong antimicrobial ability against both Escherichia coli and Staphylococcus aureus . The CAR/AgNPs/GSE film displayed a distinctive color change corresponding to changes in the total volatile basic nitrogen (TVB-N) of fish during storage. In addition, the CAR/AgNPs/GSE film showed excellent color stability to consecutive UV exposure and its storage time at 25 °C is expected to be at least 240 days, which indicates that it has high potential as an intelligent food freshness indicator film.
研究领域:
食品科学
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材料科学
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生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
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2018
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IF 3.197
Zhaoliang Wu,Na Li,Xin Zhang,Yanyan Xu,Ting Shu,Wei Liu,Bin Hu
Resveratrol (2,5,4′-trihydroxystilbene) is a naturally occurring phytoalexin produced by some spermatogphytes and it has recently attracted the increasing attention of agronomists and pharmacologists owing to its satisfactory bioactivities. The objective of this work was to develop a cost-effective technology of foam fractionation to recover trans -resveratrol ( trans -Res) from the leaching solution of muscat grape pomace on the assistance of molecular imprinting modified SiO 2 nanoparticles . First, trans -Res was leached from muscat grape pomace through the method of alkaline extraction. Under the suitable leaching conditions, the leaching yield of trans -Res reached 84.34%. Adsorption experiments were performed for describing the adsorption behavior of trans -Res on the molecular imprinting modified SiO 2 nanoparticles . Results indicated that their adsorption could be fairly described by Freundlich model and fitted well with the pseudo-second-order kinetics model. Moreover, the molecular imprinting modified SiO 2 nanoparticles could effectively maintain the stability of trans -Res through avoiding the cis-trans photo-isomerization and oxidative degradation. Finally, foam fractionation was carried out for recovering trans -Res from the leaching solution, in which the concentration of trans -Res was as high as 29.09 mg/L. The molecular imprinting modified SiO 2 nanoparticles were used as the collector. Under the suitable conditions of loading liquid volume 250 mL, particle size of molecular imprinting modified SiO 2 nanoparticles 436.0 nm, sapindus saponin concentration 0.075 g/L, volumetric gas flow rate 100 mL/min and pore diameter of gas distributor 0.180 mm, the enrichment ratio and the recovery percentage of trans -Res reached 13.68 ± 1.03 and 89.73 ± 3.57%, respectively. This work is expected to provide a new technology for selectively recovering non-surface-active materials from the solution with their isomers or analogues, and to facilitate the industrialization of foam fractionation.
研究领域:
食品科学
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生物化学
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药理学
✓ 已验证
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2018
·Vol. 41
·期 17
·3496-3502
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IF 2.415
Chunxia Lu,Zonggui Tang,Changbin Liu,Xiaomei Ma
Surface molecularly imprinted polymers were successfully prepared by a novel two-step precipitation polymerization method. The first-step allowed the formation of 4-vinylpyridine divinylbenzene and trimethylolpropane trimethacrylate copolymeric microspheres. In the second-step precipitation polymerization, microspheres were modified with a molecularly imprinting layer of oleanolic acid as template, methacrylic acid as functional monomer, and divinylbenzene/ethylene glycol dimethacrylate as cross-linker. The obtained polymers had an average diameter of 4.43 μm and a polydispersity index of 1.011; adsorption equilibrium was achieved within 40 min, with adsorption capacity reaching 27.4 mg/g. Subsequently, the polymers were successfully applied as the adsorbents of molecularly imprinted solid-phase extraction to separate and purify the oleanolic acid from grape pomace. The content of oleanolic acid in the grape pomace extract was enhanced from 13.4 to 93.2% after using the molecularly imprinted solid-phase extraction process. This work provides an efficient way for effective oleanolic acid separation and enrichment from complex matrices, which is especially valuable in industrial production.
研究领域:
化学工程
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分离技术
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天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
·
IF 6
Zhongxu Li,Ruiguo Cui,Weiwei Liu,Mengshi Wang,Lilang Li,Fengjuan Liu,Bin Du,Lijun Song
The wine-making industry produces a large amount of grape pomace, which is rich in anthocyanins. To avoid wasting these resources, choline chloride: lactic acid (molar ratio of 1:2) (DES-1) was selected as the optimal solvent. The optimal extraction conditions were as follows: temperature 60°C, time 60 min, water content 25%. The anthocyanins yield was 5.73 mg (cyanidin-3-glucoside) equivalent/ g under the optimal conditions, which was significantly higher than that obtained by DES-3 (Choline chloride: 1,4 butanediol), DES-5 (Choline chloride: 1,2 propylene glycol), and ethanol. The DES-1 extract showed significant higher color stability and antioxidant activities compared with the anthocyanins extracted by DES-3, DES-5, and ethanol. A total of eight individual anthocyanin compounds were identified in each extract, with delphinidin 3-O-glucoside, peonidin 3-O- glucoside, petunidin, and delphinidin as the dominant compounds. Molecular dynamics simulation confirmed that DES-1 system showed a larger average number of hydrogen bonds (11.63), average lifetime of hydrogen bonds (364.21 ps) and with a reduced total interaction energy (-706.82 kJ/moL), thus improving the extraction efficiency and stability of anthocyanins. This study would provide new protocols for the extraction of anthocyanins from grape pomace.
研究领域:
食品科学
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化学工程
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分子生物学
·
可持续化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）