Foods
·
2023
·Vol. 12
·期 17
·3314
·
IF 5.2
Yannan Chen,Siyuan Fei,Xiaoting Yu,Mingqian Tan
Dandelion has received wide attention in food and medicine fields due to its excellent antioxidant properties. Nonetheless, the underlying mechanism of this action has not yet been fully clarified, particularly at the metabolic level. Herein, the effects of dandelion extract (DE) on H2O2-induced oxidative damage was investigated. The results indicate that the DE alleviated H2O2-induced cell damage (increased by 14.5% compared to H2O2group), reduced the reactive oxygen species (ROS) level (decreased by 80.1% compared to H2O2group), maintained the mitochondrial membrane potential (MMP) level, and increased antioxidant-related enzyme activities. Importantly, the metabolic response of PC12 cells indicates that H2O2disturbed phospholipid metabolism and damaged cell membrane integrity. In addition, energy metabolism, the central nervous system, and the antioxidant-related metabolism pathway were perturbed. In contrast, DE rescued the H2O2-induced metabolic disorder and further alleviated oxidative damage. Collectively, these findings provide valuable stepping stones for a discussion of the mechanism and show the promise of DE as a suitable additive for functional food products.Keywords:dandelion;antioxidant;oxidative damage;metabolomics;lipidomics
研究领域:
药理学
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生物化学
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细胞生物学
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2022
·Vol. 16
·期 4
·2811-2819
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IF 3.006
Wu Ping,Ben Tingting,Zou Hui,Chen Yilun
The phenolic compounds in dandelion were extracted using different ethanol percentage solutions, identified with HPLC-MS, and their scavenging capabilities of DPPH, ABTS and OH radicals were determined. Then the excitation-emission matrix fluorescence spectroscopy coupled with parallel factor analysis (PARAFAC) was conveyed to analyze the relationship between phenolics, components scores of PARAFAC model and antioxidant capacities, based on linear regression method. The results showed that the relative content of chicoric acid, esculetin, caffeic acid, gallic acid monohydrate, eupatilin, caffeic acid-3-glucoside, corchorifatty acid F, and luteolin was higher than 0.5%, and the extraction solutions with 100% and 75% water had a better scavenging capacity of DPPH, ABTS and OH radicals. Two components PARAFAC model was identified with the comparatively higher sum of squares, core consistency values, and lower interactions numbers, and the established equations indicated the component scores had a linear regression relationship with antioxidant capacities of DPPH, and ABTS. The paper was proposed for the first time that the component scores of PARAFAC model might be treated as a useful indication for antioxidant capacity evaluation.
研究领域:
植物化学
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分析化学
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食品科学
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DOI
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2025
·Vol. 2025
·期 1
·4077536
·
IF 4.2
Jing Chen,Qinggui Lei,Weiqi Yang,Wenhao Wang,Guang’ e Tan,Zhengming Qian
Dandelion is a functional food that serves both medicinal and dietary purposes, which requires quality control to ensure its safety and efficacy. This study established a rapid high-performance liquid chromatography-ultraviolet (HPLC-UV) method for analyzing four organic acids in different parts of dandelion. After ultrasonic extraction with 40% methanol, samples were analyzed using an Agilent InfinityLab Poroshell 120 EC-C 18 column with a mobile phase of 0.1% formic acid aqueous-methanol and detection wavelength at 325 nm. The method completed the analysis in 5 min (2 min for extraction and 3 min for chromatographic analysis). The 4 organic acid standards (caftaric acid, chlorogenic acid, caffeic acid, and chicoric acid) showed excellent linearity ( r > 0.9995), and the average spiked recoveries were 101.38%, 96.85%, 103.94%, and 100.15% (relative standard deviations were 1.00%, 1.00%, 0.97%, and 0.55%), respectively. The total content of 4 organic acids is highest in dandelion flowers (1.355 mg/g dry weight [DW]), followed by stems/leaves (1.182 mg/g DW), and lowest in roots (0.026 mg/g DW). Additionally, to evaluate the environmental impact of both new and reported methods, this study performed an analysis using the blue applicability grade index. The developed methodology obtained the highest score of 80.0, which indicated that the developed HPLC method was most effective and eco-friendly. In conclusion, this method is rapid, accurate, and eco-friendly, which is help to the product development and quality evaluation of dandelion.
研究领域:
植物化学
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分析化学
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食品科学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yu-Zhen Zhang,Bei Qin,Bo Zhang,Jin-Ge Ma,Ya-Qi Hu,Lu Han,Mao-Fang He,Chun-Ye Liu
Specific recognition of caffeic acid (CA) from Taraxacum mon-golicum Hand.-Mazz. was successfully performed using a new pH responsive magnetic molecularly imprinted polymers (pH-MMIPs) by simple surface molecular imprinting polymerization. The pH-MMIPs were prepared on the surface of the Fe 3 O 4 @SiO 2 @MPS particles using CA as a template, 2-(dimethylamino) ethyl methacrylate (DMA) as the pH responsive functional monomer , 4-vinylpyridine (4-VP) as an assisting functional monomer, ethylene glycol dimethyl acrylate (EGDMA) as cross-linker, 2,2′-azobisisobutyronitrile (AIBN) as initiator and methanol-H 2 O (1:1, v/v) as the porogen . The resultant polymers were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), vibrating sample magnetometry (VSM) and x-ray diffraction (XRD). The adsorption experiments revealed that the pH-MMIPs performed high adsorption ability (11.5 mg g −1 ) by changing solution pH. Successful selective adsorption of CA was achieved with distribution coefficient of 0.12 and 0.21 towards ferulic acid and chlorogenic acid . Furthermore, pH-MMIPs were employed as adsorbents for extraction and enrichment of CA from Taraxacum mon-golicum Hand.-Mazz. extract. The recoveries of CA in the Taraxacum mon-golicum Hand.-Mazz. ranged from 90.47% to 98.97%. The results proved that the polymers have the potential to provide a selective recognition of CA in complex samples by simple pH regulation.
研究领域:
植物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2021
·Vol. 32
·期 6
·899-906
·
IF 3.373
Songbao Zhang,Chao Li,Wei Gu,Rongli Qiu,Jianguo Chao,Lingfeng Pei,Lijie Ma,Yangfang Guo,Rong Tian
Introduction Dandelion ( Taraxacum mongolicum Hand.-Mazz.) is a perennial herb with diverse pharmacological effects. The development and utilization of dandelion have attracted much attention. Objectives Our aims were to provide a reference basis for the identification of the origin of dandelions and to study the influence of their origin on their quality. Methods High-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to analyze metabolites from dandelions from four different geographical regions in China, namely Gansu, Henan, Shanxi, and Jiangsu. Metabolite analysis was performed using orthogonal partial least-squares discriminant analysis, and to identify potential metabolic pathways, MBRole was used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Results Principal component analysis revealed that the chemical components of dandelions sampled from the four regions showed noticeable differences. Twenty-six, six, six, eight, eight, and fifteen differentially produced metabolites were identified upon comparison between Gansu and Jiangsu, Gansu and Shanxi, Gansu and Henan, Henan and Shanxi, Henan and Jiangsu, and Shanxi and Jiangsu, respectively. These differentially produced metabolites were mainly phenolic compounds. Further, KEGG pathway enrichment analysis showed that the main metabolic pathways involved were biosynthesis of phenylpropanoids and flavonoids. Conclusion The methods reported herein can be used to identify the origin of dandelions; moreover, our results can serve as a reference basis for future studies.
研究领域:
植物学
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药理学
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分析化学
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
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2024
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IF 3.2
Hongkun Xue,Lamu Nima,Shuhe Wang,Jiaqi Tan
Ultrasound assisted hot water extraction (UAHWE) was applied to extraction of polysaccharides from Taraxacum mongolicum with hot water as extract solvent. Experimental factors in UAHWE process were optimized by response surface methodology. The optimal extraction parameters to achieve the highest Taraxacum mongolicum polysaccharides (TMPs) yield (12.08 ± 0.14)% by UAHWE were obtained under the ultrasound power of 200 W, extraction temperature of 62°C, solid-to-liquid ratio of 1:20 g/mL, and extraction time of 40 min, and then the crude TMPs were further purified by DEAE-52 and Sephadex G-100 chromatography to obtain a homogenous polysaccharide fraction (TMPs-1-SG). Subsequently, the structure of TMPs-1-SG was characterized by UV-vis, Fourier transform infrared spectroscopy (FT–IR), high performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), scanning electron microscope (SEM), transmission electron microscopy (TEM), and Congo red test. The results display that TMPs-1-SG with an average molecular weight of 5.49 × 10 4 Da was comprised of mannose (Man), galactose (Gal), xylose (Xyl), and arabinose (Ara) with a molar ratio of 39.85:52.61:27.14:6.30. Moreover, TMPs-1-SG did not contain a triple helix structure. Furthermore, TMPs-1-SG and TEM presented a sheet-like, rod-shaped, and irregular structure. Finally, the antioxidant activity of TMPs-1-SG was evaluated by in vitro experiment. The IC 50 values of scavenging DPPH and OH radicals for TMPs-1-SG achieved 0.71 mg/mL and 0.75 mg/mL, respectively. The findings can provide an effective method for extracting polysaccharides from natural resources.
研究领域:
植物化学
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Xingfan Li,Xinyi Du,Davey L. Jones,Zhiqiang He,Jia Liu,Xiaorui Guo,Zhonghua Tang
The widespread use of plastic products in agriculture has introduced micro-nano plastics (MNPs) and dibutyl phthalate (DBP) into soil ecosystems, disrupting microbial communities and altering metabolite profiles. However, their effects on the rhizosphere soil characteristics of medicinal plants like dandelion remain understudied. This study systematically examined the impact of PS NPs and DBP on rhizosphere microbial communities and metabolites by integrating high-throughput sequencing with liquid chromatography-mass spectrometry. Results demonstrated that individual and combined exposures to PS NPs and DBP decreased soil pH, organic matter content, and enzyme activities while reshaping the diversity, structure, and composition of rhizosphere bacteria and fungi. Notably, bacterial network stability and complexity increased under combined exposure, while fungal networks became more simplified, with a 33.72 % decrease in positive correlations. We identified potential PS NPs and DBP-degrading bacteria and biomarkers, including Nocardioides , Pseudarthrobacter , and Arenimonas . We revealed that co-exposure elevated differential soil metabolites associated with tyrosine metabolism and steroid biosynthesis. The significant positive associations between rhizosphere microorganisms and metabolites highlighted that metabolite accumulation was a key microbial response mechanism to stress. However, within the complex soil environment, the compensatory actions of microorganisms and metabolites were insufficient to mitigate the detrimental effects of PS NPs and DBP, resulting in continued inhibition of dandelion growth by 38.66 %. Consequently, these findings highlight that soil fungi and metabolism play key roles in responding to stress and influencing crop growth, providing novel insights into the impact of nanoparticle and plasticizer exposure on medicinal plant cultivation.
研究领域:
环境科学
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微生物学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2020
·Vol. 9
·期 5
·1705-1715
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IF 6.183
Sha Xiong,Jingshan Luo,Qun Wang,Zhongjun Li,Juntong Li,Qiao Liu,Liqian Gao,Shuhuan Fang,Yunyong Li,Huafeng Pan,Hong Wang,Yongbin Zhang,Qi Wang,Xiaojia Chen,Tongkai Chen
There has been an exponential increase in the rate of incidence of Parkinson's disease (PD) with aging in the global population. PD, the second most common neurodegenerative disorder, results from damaged dopamine neurons in the substantia nigra pars compacta (SNpc), along with the deposition of abnormal α-synuclein (α-Syn), and the progressive degeneration of neurons in striatal regions. Despite extensive investigations to understand the pathophysiology of PD to develop effective therapies to restrict its progression, there is currently no cure for PD. Puerarin (Pue) is a natural compound with remarkable anti-PD properties. However, its poor pharmacological properties, including poor water solubility, inadequate bioavailability, and incomplete penetration of the blood–brain barrier (BBB) have restricted its use for the treatment of PD. Nevertheless, advancements in nanotechnology have revealed the potential advantages of targeted drug delivery into the brain to treat PD. Here, we used Pue-loaded graphene oxide (GO) nanosheets, which have an excellent drug-loading ability, modifiable surface functional groups, and good biocompatibility. Then, Pue was transported across the BBB into the brain using lactoferrin (Lf) as the targeting ligand, which could bind to the vascular endothelial receptor on the BBB. In vivo and in vitro results indicated that this multifunctional brain targeted drug delivery system (Lf-GO-Pue) was an effective and safe therapy for PD.
研究领域:
神经科学
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纳米医学
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药物递送
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产品关联
DOI
PubMed
高影响因子
International Journal of Endocrinology
·
2025
·Vol. 2025
·期 1
·4473803
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IF 2.3
Song Xue,Wei Fan,Qingping Li,Hong Huang,Yibo Tang,Min Wu
Background: This study aimed to investigate the potential mechanisms of puerarin in alleviating diabetic nephropathy (DKD) in mice. Method: The DKD model was induced by multiple low-dose injections of streptozotocin (STZ) and a high-sugar and high-fat diet in male C57BL/6J mice. After confirming the onset of DKD, mice were given irbesartan, distilled water, or different concentrations of puerarin (40 and 80 mg/kg/d) by gavage for 8 weeks. HE staining and PAS staining were adopted to assess the pathological changes in the kidney tissues. Meanwhile, the levels of superoxide dismutase, catalase, creatinine, and cystatin C in the serum and the urine albumin and creatinine were measured, and the renal indices as well as the urinary albumin-to-creatinine ratio (UACR) were calculated. The changes of podocin and protein expression levels associated with AMPK/Nrf2 signaling pathway were evaluated by western blot. Results: Puerarin significantly reduced the level of fasting blood glucose, renal index, glomerular mesangial expansion index, renal function, and oxidative stress induced by STZ ( p < 0.05). The pathological injuries in kidney tissues were also alleviated. Furthermore, we demonstrated that the expression level of podocin and protein related to the AMPK/Nrf2 signaling pathway was also decreased significantly by the treatment of puerarin. At the same time, the efficacy of puerarin in the treatment of DKD was better than that of irbesartan, and the treatment effect of the high-dose group (80 mg/kg/d) was also significantly better than that of the low-dose group (40 mg/kg/d). Conclusion: Puerarin could attenuate the severity of DKD and protect the podocyte in mice in a dose-dependent way. Also, it might be performed by regulating the AMPK/Nrf2 pathway. These findings may provide a theoretical basis for updating the clinical management of DKD.
研究领域:
医学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2023
·Vol. 13
·期 1
·1-12
·
IF 4.6
Wang Rong,Li Weihua,Fang Cao,Zheng Xinxin,Liu Chao,Huang Qing
Due to the interest in the potential pharmacological application of dandelion, the chemical constituents and activities of Taraxacum mongolicum Hand.-Mazz were studied. Box–Behnken response surface methodology was employed to optimize the protocol for extraction of flavonoid from dandelion. The molecular structures of different flavonoid compounds were acquired and analyzed by liquid chromatography-mass spectrometry (LC–MS) and nuclear magnetic resonance (NMR) spectroscopy. Several major flavonoid compounds were isolated and purified, namely, hesperetin-5′-O-β-rhamnoglucoside, hesperetin-7-glucuronide, kaempferol-3-glucoside, baicalein, hyperseroside, which were extracted for the first time from dandelion. Hesperetin-5′-O-β-rhamnoglucoside was identified as a new type of flavonoid that had never reported in the literature. This new flavonoid has outstanding antioxidant activity, as shown by its IC 50 value (8.72 mg/L) for scavenging DPPH free radicals. The determination of the structure-related antioxidant activities could be interpreted based on DFT calculations. As such, we have not only illustrated the rich flavonoid contents in Taraxacum mongolicum Hand.-Mazz , but also revealed new types of flavonoid compounds in dandelion in terms of structure and antioxidant properties.
研究领域:
植物化学
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药理学
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分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xianghong Xie,Tong Bu,Qianyue Zhu,Libin Ma,Zihan Gao,Ting Du,Shuwen Liu,Jianlong Wang
The bactericidal properties of traditional food coatings mostly depend on the amount of fungicides present, which reduces the sustainability of food packaging. Herein, we proposed a magnetic field to precisely modulate the near-infrared (NIR) absorption activity to enhance antimicrobial coatings sustainability. Inspired by the typical grinding procedure, the assembly of CP/Fe 3 O 4 @TA nanofiber hydrogel was proposed as the coating, applying mechanical force and encouraging the collision of effective molecules of puerarin (PUE), chitosan (CS), and Fe 3 O 4 @TA NFs. This hydrogel design offers precise control over the physical and chemical properties, including appearance, viscoelasticity, and rheology. Particularly, significant changes in photothermal performance were observed as a result of magnetic regulation of NIR absorption activity. As a result, the CP/Fe 3 O 4 @TA coatings achieve effective bacteria killing performance under NIR irradiation, magnetocaloric effect, boric acid adsorption, and aggregation interference. Finally, the hydrogel coating was applied to the beef surface and serves as an effective barrier against the growth of pathogenic bacteria, thereby preserving the freshness and tenderness of the beef. The finding from this work is expected to open up a new way in active nano hydrogel coating for food preservation.
研究领域:
食品科学
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Mengge Shang,Jing Zhang,Xiaochan Liu,Yi Liu,Sipeng Guo,Shimo Yu,Serguei Filatov,Xibin Yi
A novel N, S self-doped hollow-sphere porous carbon is directly prepared from abundant biomass puffball spores via a simple carbonization and KOH activation process for the first time. The electrochemical properties and porous structure of these carbons are explored by adjusting the amount of KOH. It is found that the obtained N, S self-doped hollow-sphere porous carbon with a KOH to carbon mass ratio of 4 (NS-HPC-4) exhibits the largest specific surface area (1431 m 2 /g), a well-developed hierarchical porous structure and moderate N/S contents. As a result, the NS-HPC-4 electrode in the supercapacitor delivers a large specific capacitance of 285 F/g at 0.5 A/g in KOH electrolyte. Furthermore, the symmetric supercapacitor based on NS-HPC-4 exhibits an energy density of 10.9 Wh/kg at a power density of 400.4 W/kg in 2 M KOH aqueous electrolyte, and achieves a high energy density of 58.4 Wh/kg at a high power density of 876.0 W/kg in 1 M Et 4 NBF 4 /AN organic electrolyte. These excellent results confirm that puffball spores derived porous carbons are promising candidates to be used in high performance supercapacitors.
研究领域:
材料科学
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能源存储
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生物质利用
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）