Kangjing Liu,Yemeng Shen,Shiguo Chen,Wentao Su,Yukun Song,Mingqian Tan
Growing concern about the prolonged exposure to blue light on eye health has driven interest in bioactive substances that can help protect the eyes. In this study, a type of mitochondrion targeted lutein nanoparticles with potential of hydrogen (pH) and reactive oxygen species (ROS)-responsive capabilities was designed and prepared for retinal degeneration caused by blue light. Lutein nanoparticles were initially prepared using a self-assembly method based on (3-carboxypentyl) (triphenyl) phosphonium modified casein-mannose (CAS-Man) conjugates. Subsequently, 3-aminobenzeneboronic acid modified sodium alginate was surrounded on the surface of the nanoparticles to generate hydrophilic corona. The nanoparticles exhibited pH- and ROS-responsive release properties and showed good mitochondrial targeted ability after 4 h of incubation, with a Pearson’s correlation coefficient of 0.88. Visual electrophysiology results indicated that lutein nanoparticles alleviated blue light damage by improving dark and light adaptation, preserving retinal vascular microcirculation, and enhancing optic nerve conduction function. Fluorescein fundus angiography showed that lutein nanoparticles significantly decreased the vessel percentage area by 6.93 ± 0.32%, average vessel length by 0.59-fold, and the number of junctions by 0.88-fold caused by blue light exposure. Lutein nanoparticles effectively protected mice from blue light-induced retinal degeneration by reducing ROS production in the retina by 1.44-fold and inhibiting cellular apoptosis by 2.29-fold. These findings suggested that the mitochondrion targeted lutein nanoparticles with pH and ROS-responsive capabilities provided a potential strategy for mitigating blue light-induced retinal degeneration.
Di Peng,Shengan He,Yuyan Zhang,Liqin Yao,Wendong Nie,Zhijian Liao,Wenting Cai,Xinyu Ye
The demand for in-situ detection of latent fingerprints (LFPs) in ways of high sensitivity, high selectivity, high contrast, low cost and user-friendly is still urgent. To overcome this challenge, a moisture-stable, red-emitting fluoride phosphor K 3 AlF 6 :Mn 4+ (KAF:Mn 4+ ) with an organic hydrophobic skin was prepared. The phosphor has a uniform and superfine morphology with excellent luminescence properties. More importantly, this non-ultraviolet (UV) or non-near infrared (NIR) induced phosphor was proved to be an ideal fluorescent label for LFP imaging, which is both friendly for touch DNA analysis and compatible to forensic light sources. The well-defined ridge details with little background interference on various surfaces were presented by the oleic acid (OA) modified KAF:Mn 4+ (KAF:Mn 4+ -OA) phosphor in few seconds using the powder dusting method. To confirm the high selectivity of KAF:Mn 4+ -OA for LFP imaging, an efficient quantitative evaluation method is proposed with the aid of ImageJ & Origin software. Due to the superiority of the Mn 4+ -doped fluoride for the rapid imaging of LFPs in terms of low-cost, high compatibility and good availability, it is expected to be a promising candidate for forensic science as well as fluorescence imaging in other fields instead of rare earth luminescent materials.
危险品化学品经营许可证（带存储）