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Foods · 2024 ·Vol. 13 ·期 8 ·1240 · IF 4.7

Evaluation of Blue Honeysuckle Berries (Lonicera caerulea L.) Dried at Different Temperatures: Basic Quality, Sensory Attributes, Bioactive Compounds, and In Vitro Antioxidant Activity

Min Yu,Beibei Wang,Zhiqiang Huang,Jinjiao Lv,Yunfei Teng,Tianbo Li,Yu Zhang,Kun Dong,Dong Qin,Junwei Huo,Chenqiao Zhu

This study aims to comprehensively investigate the effects of hot-air dehydration on the quality of blue honeysuckle berries (Lonicera caeruleaL.). The results demonstrated that drying with hot air at 40–65 °C for 7–72 h resulted in blue honeysuckle berries with a moisture content of 0.21–1.10 g H2O/g dry weight. Generally, low to medium temperatures (40–55 °C) showed a better effect on the quality than high temperatures (60–65 °C). Specifically, drying at 40 °C exclusively resulted in better retention of cuticular wax, the best sensory appearance, and the highest total phenolic content. Drying at 45 °C and 50 °C resulted in the highest antioxidant capacity and the optimal sensory flavor. Drying at 55 °C led to the highest soluble solid/acid ratio, ascorbic acid concentration, total flavonoid, and total anthocyanin. The work introduces an innovative raw berry product and provides a comprehensive practical and theoretical framework for convective dehydration of blue honeysuckle berries.

研究领域: 食品科学 · 植物学
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MEDICINAL CHEMISTRY RESEARCH · 2025 · IF 3.1

Identification of the chemical composition of blue honeyberry leaves (Lonicera caerulea L. var. edulis) and their anti-inflammatory activity

Rong Zhenji,Yu Chunping,Wang Xin,Wang Ruyue,Gao Yang,Zhang Hailong

Blue honeyberry is a well-known nutritious fruit. Meanwhile, it is also an Oroqen herb in China. However, the chemical constituent of the leaves of the plant is little known, despite having better anti-inflammatory activity. In this study, we investigated the chemical constituents of blue honeyberry leaves and screened their anti-inflammatory effects in RAW264.7 cells. Thirty-nine compounds including a new fernane-type triterpenoid were isolated and identified using various chromatographic approaches and spectroscopic techniques. The new compound and some other components showed a significant anti-inflammatory effect by inhibiting nitric oxide production in RAW264.7 cells, in which compounds 6 and 38 exhibited the inhibition of 40.6 ± 4.8% and 46.0 ± 1.4% at the concentration of 100 μM, respectively, without inducing any cytotoxicity.

研究领域: 植物化学 · 药理学 · 天然产物研究
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FOOD CHEMISTRY · 2023 · IF 8.8

Effects of extraction methods on the structure and functional properties of soluble dietary fiber from blue honeysuckle (Lonicera caerulea L.) berry

Run Ji,Xiuling Zhang,Chenghai Liu,Wentao Zhang,Xiaofeng Han,Hengtian Zhao

The work within this study aimed to investigate and compare the effects of compound enzyme extraction (CE), ultrasonic chemical extraction (UC) and combined fermentation extraction (CF) on the physicochemical properties, microstructure, and functional properties of soluble dietary fiber (SDF) extracted from blue honeysuckle berries. The results showed that CE-SDF had higher crystallinity (32.41%). UC-SDF had the highest yield (13.32 ± 0.80 g/100 g). CF-SDF had the maximum inhibition of α-amylase (50.82 ± 0.76%) and α-glucosidase (54.87 ± 1.25%). The in vitro hypoglycemic activity of the three SDFs was observed in the order of CF > CE > UC. Meanwhile, the purity of SDF had a strong positive correlation with its antioxidant and in vitro hypoglycemic capacities. The crystallinity of SDF was found to be positively correlated with its molecular weight and thermal properties. Additionally, the sugar composition of SDF was found to be an important factor affecting its biological activity.

研究领域: 食品科学 · 植物化学 · 营养学
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Food and Bioprocess Technology · 2023 · IF 5.6

Enhancement of Haskap Vacuum Freeze-Drying Efficiency and Quality Attributes Using Cold Plasma Pretreatment

Li Jiangfei,Zhou Yajun,Lu Weihong

Vacuum freeze drying (VFD) has been widely used for drying berries, by combining with pretreatment technology, energy consumption and cost can be effectively reduced as well as product quality improved. However, the effect of cold plasma pretreatment (CPP) on the drying efficiency and quality characteristics of VFD haskap has not been investigated. In this study, an innovative drying technology, cold plasma pretreatment–assisted vacuum freeze drying, was applied to process haskap. The purpose of the present study was to explore the effect of different times of CPP (0–75 s) on VFD haskap performance. The results indicated that the application of CPP enhanced the effective diffusion rate and drying rate (27.68%) owing to pretreatment etching larger and more cracks in the microstructure, increasing cell membrane permeability (15.59%), and altering cell wall pectin composition. Midilli is the most recommended model for drying kinetics. CPP contributed to increased hydrophilicity (25.33%) and degraded enzymatic activity, thereby prompting the enhancement of polyphenols, anthocyanins, flavonoids, and antioxidant capacity. Besides, the physico-chemical properties were ameliorated, the possible mechanism for the enhanced drying rate and quality properties of VFD haskap by CPP was explained by chemometrics. Overall, CPP is a promising pretreatment technology for haskap VFD.

CAS / MDL: 149-91-7 153-18-4
研究领域: 食品科学 · 农产品加工
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2022 · IF 8.025

Preparation, characterization and bioactivities of selenized polysaccharides from Lonicera caerulea L. fruits

Chuntian Shao,Jingwei Zhong,Junwen Liu,Yiyan Yang,Meilin Li,Yang Yu,Yaqin Xu,Libo Wang

Native polysaccharide was obtained from Lonicera caerulea L. fruits (PLP). Two selenized polysaccharides (PSLP-1 and PSLP-2) were synthesized by the microwave-assisted HNO 3 -Na 2 SeO 3 method, where the selenium (Se) contents were 228 ± 24 and 353 ± 36 μg/g, respectively. The molecular weights of PLP, PSLP-1, and PSLP-2 were 5.9 × 10 4 , 5.6 × 10 4 , and 5.1 × 10 4  kDa, respectively. PSLP-1 and PSLP-2 contained the same type of monosaccharides as PLP but with different molar ratios. The main chain structure of the native polysaccharide was not changed after selenization. PLP, PSLP-1, and PSLP-2 contained the same six types of glycosidic bonds. Bioactivity assays revealed that the two selenized polysaccharides possessed better antioxidant activities than PLP, but their bile acid-binding abilities and inhibitory activities on acetylcholinesterase (AChE) had weakened. In summary, PLP, PSLP-1, and PSLP-2 may be promising Se supplements in functional foods and inhibitors for the treatment of AChE.

研究领域: 食品科学 · 生物化学 · 药理学
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Journal of the Taiwan Institute of Chemical Engineers · 2020 · IF 4.794

A sustainable and efficient preparation process of anthocyanins from blue honeysuckle fruit and comprehensive bioactivity assessment

Ping Kou,Ning Wan,Li-Tao Wang,Hong-Yi Pan,Jiao Jiao,Chun-Jian Zhao,Zhi-Guo Liu,Xi-Qing Wang,Yu-Jie Fu

In the present study, an integrated process for preparation of anthocyanins components from blue honeysuckle fruits using deep eutectic solvent-based negative pressure cavitation extraction (DES-NPCE) was explored rationally. Single factor optimization combined with Box-Behnken Design (BBD) were applied to confirm the influential parameters. The maximum extraction yields of total anthocyanins and main constituent cyanidin-3-glucoside (C3G) reached 6.601 mg/g and 4.839 mg/g, which were 1.197–1.565 folds of other conventional methods. Moreover, the anthocyanins products exhibited higher stability from DES-NPCE extraction than that of conventional extraction solvents , which was conducive to the preservation and utilization of anthocyanins. After enrichment with macroporous resin D101, the contents of total anthocyanins and C3G reached 25.72% and 19.05%, and the recovery yields were 81.35% and 82.24%, respectively. Furthermore, the anthocyanins products exhibited more powerful chemical and cellular antioxidant activities, which were similar or stronger than that of positive control Vc or BHT. These results revealed that the proposed process was definitely an efficient and sustainable strategy for the stabilization preparation of thermo sensitive biologically active natural products. The developed method has also proved to have sufficient potential for application in food, nutrition and pharmaceutical industries.

CAS / MDL: 67-48-1
研究领域: 食品科学 · 药学 · 天然产物化学 · 生物技术
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JOURNAL OF ETHNOPHARMACOLOGY · 2025 · IF 5.4

Lonicera caerulea var. edulis in acute lung injury therapy: integrated chemical composition, anti-inflammation, antioxidation, and NF-κB cascades based on bioactivity-guided fractionation

Ruyue Wang,Chunping Yu,Xin Wang,Minfei Yang,Yang Gao,Hailong Zhang

Ethnopharmacological relevance Lonicera caerulea var. edulis is an Oroqen medicine with fever relief, detoxification, and anti-inflammatory activities. However, pharmacological and chemical research on its leaves is limited. Aim of the study Focusing on L. caerulea var. edulis leaves, the research involved identification of active constituents, examination of anti-inflammatory effects in mice, and analysis of mechanisms. Materials and methods Based on activity-guided fractionation, the phytochemicals were separated via various chromatographic and spectroscopic techniques. The anti-inflammatory effects were evaluated in mice with LPS-stimulated acute lung injury. Antioxidant capacity was determined through DPPH • and OH • scavenging, ferric-reducing and lipid peroxidation assay. The active compounds were screened in LPS-treated RAW 264.7 macrophage cells. Then, the anti-inflammatory mechanisms were investigated via RT-qPCR, western blot analysis, and molecular docking experiments. Results The EtOAc fraction (EF) displayed the strongest anti-inflammatory activity, with a pronounced inhibitory effect on cellular NO production, markedly decreasing the levels of inflammatory factors, effectively improving acute lung injury, and alleviating pulmonary edema in mice. Meanwhile, EF was also the strongest antioxidant layer which could effectively scavenge free radicals. Phytochemical investigation of EF resulted in the isolation of one new diterpenoid and 19 known constituents, of which ten compounds are lignans. Among them, a lignan ( 8 ) significantly reduced NO production, inhibited the IL-1β, IL-6, iNOS, and COX-2 gene expression, and decreased the phosphorylation levels of p65 and IκBα in RAW 264.7 macrophages. Additionally, 8 could tightly interact with the p65 and IκBα proteins of NF-κB. Conclusion The leaves of L . caerulea var. edulis have significant anti-inflammatory properties, and lignans are the main active components, whose anti-inflammatory mechanisms are mainly through inhibiting the NF-κB signaling pathway.

CAS / MDL: 50-81-7
研究领域: 植物化学 · 药理学 · 民族药理学 · 炎症研究 · 分子生物学
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