Biochimica et Biophysica Acta-Gene Regulatory Mechanisms
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2018
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IF 5.179
Quan Gan,Xinwei Zhang,Daobo Zhang,Liang Shi,Yue Zhou,Tongtong Sun,Song Jiang,Junshan Gao,Yan Meng
Sucrose is the most commonly transported sugar in plants and is easily assimilated by insects to fulfill the requirement of physiological metabolism. BmSuc1 is a novel animal β-fructofuranosidase (β-FFase, EC 3.2.1.26) - encoding gene that was firstly cloned and identified in silkworm , Bombyx mori . BmSUC1 was presumed to play an important role in the silkworm-mulberry enzymatic adaptation system by effectively hydrolyzing sucrose absorbed from mulberry leaves. However, this has not been proved with direct evidence thus far. In this study, we investigated sucrose hydrolysis activity in the larval midgut of B. mori by inhibition tests and found that sucrase activity mainly stemmed from β-FFase, not α-glucosidase. Next, we performed shRNA-mediated transgenic RNAi to analyze the growth characteristics of silkworm larvae and variations in glycometabolism in vivo in transgenic silkworms. The results showed that in the RNAi- BmSuc1 transgenic line, larval development was delayed, and their body size was markedly reduced. Finally, the activity of several disaccharidases alone in the midgut and the sugar distribution, total sugar and glycogen in the midgut, hemolymph and fat body were then determined and compared. Our results demonstrated that silencing BmSuc1 significantly reduced glucose and apparently activated maltase and trehalase in the midgut. Together with a clear decrease in both glycogen and trehalose in the fat body, we conclude that BmSUC1 acts as an essential sucrase by directly modulating the degree of sucrose hydrolysis in the silkworm larval midgut, and insufficient sugar storage in the fat body may be responsible for larval malnutrition and abnormal petite phenotypes.
研究领域:
昆虫学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2016
·
IF 3.926
Wenwu Yan,Nani Wang,Peimin Zhang,Jiajie Zhang,Shuchao Wu,Yan Zhu
A standard-free and sensitive method was developed for analysis of sucrose acetates in a crude 6- O -acetyl sucrose (S-6-a) product by on-line hydrolysis-high-performance liquid chromatography with pulsed amperometric detection (PAD). Sucrose, three regio-isomers of acetyl sucrose and five regio-isomers of diacetyl sucrose were separated on a C18 column using 3% (v/v) acetonitrile in water as eluent within 25 min. After purification with LC followed by semi-preparative HPLC, their chemical structures were identified by 1D, 2D NMR and LC–MS. Moreover, quantification of those regio-isomers was achieved by on-line alkaline hydrolysis to liberate sucrose using a post-column delivery system, and then detected by PAD for indirect estimation of the sucrose acetate content. Under optimal conditions, the linear ranges were from 0.03 to 150 μmol L −1 for sucrose corresponding to sucrose acetates with coefficient of determination as 0.9997 and detection limit as 0.01 μmol L −1 ( S/N = 3). Good repeatability was obtained (RSD < 3%, n = 6). Furthermore, this method has been successfully applied to the analysis of sucrose and sucrose acetates in a crude S-6-a product during synthesis, purification and structure elucidation studies. The recoveries were from 94.89% to 102.31% for sucrose and sucrose acetates.
研究领域:
分析化学
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食品科学
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有机化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2017
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IF 4.529
Lingtian Wu,Shanshan Wu,Juanjuan Qiu,Chuanxue Xu,Sha Li,Hong Xu
A green process and environmentally benign process is highly desirable in the development of enzymatic catalysis. In this work, the shuttle plasmid pHA01 was constructed and the sucrose isomerase (SIase) was expressed in Bacillus subtilis WB800. The optimal nitrogen and carbon sources for SIase expression were yeast extract (15 g/L) and un-pretreated cane molasses (UCM, 20 g/L), respectively. After the UCM fed, the whole cell activity reached 5.2 U/mL in a 7.5 L fermentor. Optimum catalytic temperature and pH of whole cell were 35 °C and 5.5, respectively. Although the biologic membrane reactor (BMR) system consecutively worked for 12 batches, the sucrose conversion remained higher than 90%, indicating the BMR system had a greater operational stability. Furthermore, isomaltulose production using the BMR system with low-cost cane molasses as its substrate not only reduces the production cost and mediates environmental pollution, but also solves the genetic background problem of the non-food-grade strains.
研究领域:
生物技术
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酶工程
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可持续化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
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IF 5.895
Jianlin Chu,Yani Tian,Qian Li,Gaofei Liu,Qi Yu,Tianyue Jiang,Bingfang He
Levan-type fructooligosaccharides (FOS) exhibit enhanced health-promoting prebiotic effects on gut microbiota. The wild type (WT) of β-fructofuranosidase Fru6 could mainly yield 6-ketose. Semirational design and mutagenesis of Fru6 were exploited to promote the transfructosylating capacity for FOS. The promising variants not only improved the formation of 6-kestose but also newly produced tetrasaccharides of 6,6-nystose and 1,6-nystose (a new type of FOS), and combinatorial mutation boosted the production of 6-kestose and tetrasaccharides (39.9 g/L 6,6-nystose and 4.6 g/L 1,6-nystose). Molecular docking and molecular dynamics (MD) simulation confirmed that the mutated positions reshaped the pocket of Fru6 to accommodate bulky 6-kestose in a reactive conformation with better accessibility for tetrasaccharides formation. Using favored conditions, the variant S165A/H357A could yield 6-kestose up to 335 g/L, and tetrasaccharides (6,6-nystose and 1,6-nystose) reached a high level of 121.1 g/L (134.5 times of the mutant S423A). The β-(2,6)-linked FOS may show the potential application for the prebiotic ingredients.
研究领域:
酶工程
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食品科学
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微生物学
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生物技术
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DOI
PubMed
高影响因子
Cell Reports Physical Science
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2024
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IF 7.9
Wei-Ming Yin,Ben Dang,Yuanyuan Miao,Shujun Li,Jian Li,Shouxin Liu,Tony D. James,Zhijun Chen
Producing room temperature phosphorescent materials from natural sources is essential for sustainability. However, either toxic chemical reagents or complicated processing is still required to produce such phosphorescent materials derived from natural resources. Here, we produce sustainable room temperature phosphorescence (RTP) materials from wood and sugar. Specifically, sugar is introduced into a wood matrix, which confines the lignin in the wood matrix in situ . As a result, the treated wood (sucrose wood [S-wood]) exhibited RTP emissions with a lifetime of ∼284.4 ms. Life cycle carbon analysis indicates that ∼8.1–11.2 kg CO 2 is produced during the preparation of S-wood, which is lower than for other similar materials. Moreover, the S-wood could be naturally degraded in the soil over 40 days. As a demonstration of the practical potential, S-wood is used for visual decorations and anti-counterfeiting applications.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
iScience
·
2024
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IF 4.6
Zizhang Wang,Hao Li,Yuxiang Weng
Previously, OsNIN8 initiated sucrose signal for cell division in radicle and seed development in rice. Here, a set of genes were induced in starved sprouts after sucrose treatment and 14 genes were screened between ZH11 and nin8 as reporters of sucrose signal. Expressions of reporter depended on levels of OsNIN8 in overexpression and RNAi lines. Further, OsNIN8 interacted with OsFKBP12, a regulator of TOR signal for cell division, and OsFKBP12 interacted with OsTOR (OsTORKD). However, interactions of OsFKBP12 with OsNIN8 or OsTORKD were a loose binding depending on hydrophobicity of OsFKBP12 C-terminus in Y2H. In addition, OsFKBP12 associating with OsNIN8 was endothermic but with OsNIN8m was exothermic. Knockout OsFKBP12 reappeared nin8 phenotypes and complementation the knockout with C-termini of OsFKBP12 worsened the phenotypes. Treatment with TOR inhibitors caused short radicle and OsTOR RNAi repeated low seed-setting of the phenotypes. So, OsFKBP12 transduced sucrose signal from OsNIN8 to TOR pathway.
研究领域:
植物学
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分子生物学
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遗传学
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产品关联
DOI
PubMed
高影响因子
Food & Function
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2021
·Vol. 12
·期 20
·9912-9921
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IF 5.396
Mohan Zhang,Shuai Chen,Yuhua Dai,Ting Duan,Yuying Xu,Xiaolin Li,Jun Yang,Xinqiang Zhu
Aspartame (ASP) and sucralose (SUC) are non-nutritive sweeteners which are widely consumed worldwide. They are considered safe for human consumption, but their effects on certain physiological aspects, such as the lifespan or health status, of the organism have not yet been studied in depth and only limited data are available in the literature. The objectives of this study were to evaluate the effects of ASP and SUC on the lifespan and health indexes using Caenorhabditis elegans (C. elegans) as a model system. Interestingly, it was shown that at the concentrations tested, ASP (0.03–3 mg mL−1) showed an increasing trend of the mean lifespan of C. elegans, with a significant increase of 27.6% compared to the control at 3 mg mL−1. Similarly, SUC (ranging from 0.03 to 10 mg mL−1) also significantly increased the mean lifespan by 20.3% and 22.3% at 0.03 and 0.3 mg mL−1, respectively. However, 10 mg mL−1 SUC had a negative effect on the lifespan, though it did not reach a statistically significant level. In addition, ASP and SUC decreased lipofuscin accumulation and transiently improved motility, indicating improved health status. Nonetheless, they had different effects on food intake and intestinal fat deposition (IFD) at different intervals of time. Taken together, our findings revealed that ASP and SUC can prolong the lifespan and improve the health status of C. elegans.
研究领域:
生物学
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营养学
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毒理学
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产品关联
DOI
PubMed
高影响因子
METABOLIC ENGINEERING
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2013
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IF 6.859
Wei Du,Feiyan Liang,Yangkai Duan,Xiaoming Tan,Xuefeng Lu
Because cyanobacteria are photosynthetic, fast-growing microorganisms that can accumulate sucrose under salt stress, they have a potential application as a sugar source for the biomass-derived production of renewable fuels and chemicals. In the present study, the production of sucrose by the cyanobacteria Synechocystis sp. PCC6803, Synechococcus elongatus PCC7942, and Anabaena sp. PCC7120 was examined. The three species displayed different growth curves and intracellular sucrose accumulation rates in response to NaCl. Synechocystis sp. PCC6803 was used to examine the impact of modifying the metabolic pathway on the levels of sucrose production. The co-overexpression of sps ( slr0045 ) , spp ( slr0953 ), and ugp ( slr0207 ) lead to a 2-fold increase in intracellular sucrose accumulation, whereas knockout of ggpS ( sll1566 ) resulted in a 1.5-fold increase in the production of this sugar. When combined, these genetic modifications resulted in a fourfold increase in intracellular sucrose accumulation. To explore methods for optimizing the transport of the intracellular sucrose to the growth medium, the acid-wash technique and the CscB (sucrose permease)-dependent export method were evaluated using Synechocystis sp. PCC6803. Whereas the acid-wash technique proved to be effective, the CscB-dependent export method was not effective. Taken together, these results suggest that using genetic engineering, photosynthetic cyanobacteria can be optimized for efficient sucrose production.
研究领域:
微生物学
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遗传学
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生物工程
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产品关联
DOI
PubMed
高影响因子
ADVANCED SYNTHESIS & CATALYSIS
·
2016
·Vol. 358
·期 24
·4030-4040
·
IF 6.453
Lingtian Wu,Juanjuan Qiu,Shanshan Wu,Xiaoliu Liu,Chao Liu,Zheng Xu,Sha Li,Hong Xu
Isomaltulose is a new functional sweetener that possesses biological characteristics and can be obtained by enzymatic synthesis using sucrose as substrate. In this study, the sucrose isomerase (SIase) was successfully immobilized on the sponge synthesized with ϵ-poly- l -lysine and gelatin. The inhibition zones proved that the sponge possessed a significant bacteriostatic function, which can effectively prevent the collapse of the scaffolds of the immobilized enzymes. The immobilized SIase activity reached up to 71.58 U g −1 (the SIase loading was about 153.43 mg g −1 ), and the activity recovery was approximately 84.50%. After immobilization, the optimum pH of SIase decreased from 6.0 to 5.5 and the optimum temperature increased from 30 °C to 40 °C. The affinity of SIase to substrate was basically unchanged. Immobilized SIase still exhibited more than 90% sucrose conversion after 13 consecutive cycles, which indicated that it had a good operational stability. Furthermore, the immobilized SIase has the potential for isomaltulose production, with 200 g L −1 sucrose solution as its substrate in the food industry. Isomaltulose was isolated in 83.58% yield and high purity (97.3%).
研究领域:
生物化学
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食品科学
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
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2015
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IF 4.642
Yawei Shi,Xiangwen Zhang,Guozhu Liu
Activated carbons derived from hydrothermal carbonization of sucrose and subsequent KOH activation have been prepared and tested for the adsorptive removal of refractory thiophenic compounds. Textural and chemical properties of the carbons and their corresponding impacts on adsorption rates and capacities were discussed in detail. The optimum carbon possessed high adsorption capacity (41.5 mgS/g for 300 ppmwS model oil), fast adsorption rate (97% saturated within 5 min) as well as relatively good selectivity for the adsorption of thiophenic compounds due to the abundant small micropores, suitable mesopore fraction and various oxygen functionalities present in the carbon. Combined with the economic and environmental merits of the preparation procedure, the sucrose-derived activated carbons are promising candidates for potential practical applications.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
International Journal of Chemical Engineering
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2014
·
IF 0
Hu Li,Song Yang
L-Proline derived ionic liquids (ILs) used as both solvent and catalyst were efficient for transformation of fructose and sucrose to 5-hydroxymethylfurfural (HMF) in the presence of water. Response surface methodology (RSM) was employed to optimize fructose dehydration process, and a maximum HMF yield of 73.6% could be obtained at 90°C after 50 min. The recycling of the IL exhibited an almost constant activity during five successive trials, and a possible reaction mechanism for the dehydration of fructose to HMF was proposed.
研究领域:
化学工程
·
催化反应
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产品关联
DOI
CERAMICS INTERNATIONAL
·
2020
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IF 3.83
Nannan Wang,Yong Hong,Terence Xiaoteng Liu,Qi Wang,Jiarui Huang
A microporous–mesoporous carbon has been successfully prepared via carbonization of sucrose followed by heat treatment process. The obtained porous carbon possesses abundant micropores and mesopores, which can effectively increase the sulfur loading. The composite exhibited a remarkable initial capacity of 1185 mAh g −1 at 0.2 A g −1 and maintained at 488 mAh g −1 after 200 cycles, when employed for lithium‒sulfur batteries. Moreover, the composite displayed enhanced rate capabilities of 1124, 914 and 572 mAh g −1 at 0.2, 0.5 and 1.0 A g −1 . The outstanding electrochemical capabilities and facile low‒cost preparation make the new microporous–mesoporous carbon as an excellent candidate for lithium sulfur batteries .
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）