JOURNAL OF FOOD SCIENCE
·
2019
·Vol. 85
·期 1
·123-131
·
IF 2.081
Chaojun He,Ziyong Li,Hongxia Liu,Haining Zhang,Liye Wang,Hong Chen
Adlay oil (AO) is an important component of adlay seeds that has many beneficial functions to human health. In this study, the variations in the chemical composition and antioxidant activity of AOs extracted from adlay samples obtained from four main producing areas of China were first investigated. Results revealed that the AO of the adlay samples varied from 7.398 ± 0.486% to 8.464 ± 0.725%. The highest contents of total fatty acid, triolein, total phenolic, and total flavonoid were observed in Xingren AO, whereas coixol content was found to be highest in Pucheng AO. The AO samples were grouped successfully in accordance with the origins by partial least squares–discriminant analysis and hierarchical cluster analysis based on the contents of the chemical components. Furthermore, AOs exhibited considerable levels of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (IC 50 , 0.924 ± 0.113 to 1.116 ± 0.109 mg/mL) radical scavenging activity, ferric-reducing antioxidant power (FRAP) activity (EC 50 , 0.019 ± 0.002 to 0.028 ± 0.002 mg/mL) activity, and β-carotene-linoleic acid bleaching activity (IC50, 0.233 ± 0.008 to 0.414 ± 0.012 mg/mL) activity. Total phenolic and total flavonoid both demonstrated highest correlation with ABTS values ( r = −0.952 and r = −0.960, respectively). The results derived from above studies suggest that geographic origin has a certain influence on the phytochemical profiles and antioxidant activity of the AOs. Practical Application The chemical composition and antioxidant activity of adlay oils extracted from the adlay samples obtained from four main producing areas of China were evaluated, including total fatty acid, triolein, total phenolic, and total flavonoid, and ABTS radical scavenging activity, FRAP activity, and β-carotene-linoleic acid bleaching activity.
研究领域:
食品科学
·
植物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Inflammation Research
·
2025
·
IF 4.1
Yi-Xuan Zeng,Ni-Ren Li,Bing-Ying Deng,Yu-Feng Gu,Si-Fan Lu,Yi Liu
Background Ulcerative colitis is a chronic intestinal disease linked to intestinal barrier damage, ferroptosis and dysbiosis. Coix lacryma-jobi is a natural food with food-medicine homology, whose seed-derived oil (Coix seed oil, CSO) has been shown anti-inflammatory activity in vitro. Here, the effects and mechanisms of CSO on ulcerative colitis (UC) in vivo are systematically investigated.Methods Firstly, the UC mice was replicated by 3% DSS, and assessed the efficacy of CSO by observing the fecal occult blood, colon length, DAI score and pathological histomorphological changes of colon tissues. The anti-inflammatory and barrier-protective effects of CSO were observed by AB staining and qRT-PCR. Secondly, the biological targets of CSO were obtained from TCMSP database and Swiss Target Prediction database, ferroptosis targets were downloaded from FerrDb platform, and UC-related disease targets were obtained from GEO database, and the intersection of the above three was taken to obtain “CSO-UC-Ferroptosis” intersection targets, which were analysed by GO and KEGG enrichment, GSEA analysis, and immune cell infiltration and validation. Finally, the core genes of “CSO-UC-Ferroptosis” were molecular docking with the potential active components of CSO. In order to further verify the effect of CSO on ferroptosis, the GPX4 agonist RSL-3 was used to stimulate mice in vivo, and the levels of Iron, MDA and SOD were measured, and immunohistochemistry was used to detect the effects of tight junction proteins and the “CSO-UC-Ferroptosis” core protein in mice. Besides, the effect of CSO was further evaluated by observing the intercellular junctions of the colon tissues of each group under electron microscope. In addition, 16sRNA sequencing was performed on the intestinal contents of the mice to observe the effects of CSO on the intestinal flora of UC mice.Results CSO improved physiological parameters, reduced inflammation response and intestinal barrier damage, regulated ferroptosis, and restored gut microbiota balance in UC mice. Bioinformatics results showed that G6PD, ABCC1 were core targets at the intersection of CSO, UC and ferroptosis, which also demonstrated the similar expression of the core genes in DSS-induced UC mice models in vivo.Conclusion Our findings demonstrate for the first time that CSO ameliorated UC by regulating intestinal barrier damage, ferroptosis and the gut microbiota in DSS-induced mice, suggesting that CSO as a promising candidate for UC treatment and warranting further investigation.
研究领域:
药理学
·
免疫学
·
分子生物学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）