INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 2.6
Xudong Xia,Rongrui Lu,Nanxing Zhao,Hongming Kong,Fang Yuan,Hesheng Liu,Lianliang Liu,Xiangyang Qi,Qiuping Chen
Summary α-Glycosidase, a critical therapeutic target in type 2 diabetes mellitus (T2DM), can be modulated by inhibitors to reduce postprandial blood glucose levels. This study investigated the inhibitory effects of vine tea ( Ampelopsis grossedentata ) and its key compounds on α-glycosidase. The results demonstrated that fifteen varieties of vine tea extracts inhibited α-glycosidase in a dose-dependent manner, with IC 50 values ranging from 5.25 to 35.8 μg mL −1 . HPLC analysis revealed dihydromyricetin (DHM) as the main compound in vine tea, with an IC 50 value of 262.50 μM. Notably, the synergistic combination of DHM and the antidiabetic drug acarbose (ABS) significantly enhanced inhibitory effectiveness, with the optimal ratio (1:125) yielding a potentiation index (CI) of 0.09. Fluorescence and molecular docking analyses supported the hypothesis that DHM enhanced ABS's inhibitory effect through complex formation and specific interactions, offering a promising option for safer and more effective diabetes therapy.
研究领域:
药理学
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糖尿病研究
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植物化学
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产品关联
DOI
高影响因子
Heliyon
·
2023
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IF 4
Ying Li,Ming-Wang Kong,Nan Jiang,Chen Ye,Xiao-Wei Yao,Xiao-Juan Zou,Hai-Ming Hu,Hong-Tao Liu
Gut microbiota disturbance, autophagy dysregulation, and accumulation of hepatic bile acids (BAs) are essential features of liver injury. Therefore, regulating autophagy and BA metabolism are potential strategies for treating liver diseases. Vine tea has been seen beyond a pleasant tea in food science. Our previous study found that vine tea extract (VTE) intervention alleviated acute liver injury (ALI) by restoring gut microbiota dysbiosis. In this study, we aim to investigate the effect of VTE on carbon tetrachloride (CCl 4 )-induced hepatic autophagy and BA metabolism disorder in mice. The results showed that VTE effectively suppressed CCl 4 -induced liver fibrosis and hepatic autophagy. LC-MS/MS assay suggested that VTE affected fecal BA production by reducing the fecal BA levels and improving cholestasis in ALI mice. Besides, VTE inhibited BA synthesis, promoted BA transport in the liver, and enhanced BA reabsorption in the ileum through the farnesoid X receptor (FXR)-related signaling pathway. The hepatic expressions of Fxr and Abca1 were elevated by VTE. Finally, the depletion of gut microbiota in ALI mice had a negative impact on abnormal autophagy and BA metabolism. It was also noted that the administration of VTE did not provide any additional improvement in this regard. Overall, VTE ameliorated ALI by reversing hepatic autophagy and abnormal BA metabolism, and the beneficial effects of VTE on liver injury depended on the existence of gut microbiota.
研究领域:
食品科学
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肝脏病学
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微生物学
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DOI
高影响因子
RSC Advances
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2022
·Vol. 12
·期 44
·28659-28676
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IF 4.036
Liling Wang,Yanbin Wang,Meixu Chen,Yaoyao Zhu,Yuchuan Qin,Yifeng Zhou
In this study, deep eutectic solvent oscillation-assisted extraction (DES-OS) combined with macroporous resin adsorption and desorption technology was used to achieve the rapid green extraction and separation of the characteristic component dihydromyricetin (DMY) from vine tea. Multivariate data analysis showed that the DES system composed of tetrabutylammonium bromide (N444Br) and pyruvic acid (molar ratio 1 : 2) had good extraction performance for DMY. The influence parameters of DES-OS were studied, and optimized by the single-factor test and response surface methodology (RSM) with Box–Behnken design (BBD). The extraction model of DMY was established and verified. The results showed that the extraction yield of DMY could reach 40.1 mg g−1 under the optimal conditions (DES water contents of 71.18%, extraction time of 2.80 h, extraction temperature of 46.40 °C), which is in good agreement with the predicted value. In addition, Fourier transform infrared spectroscopy (FT-IR) was used to characterize the solvent before and after extraction. Scanning electron microscopy (SEM) results further confirmed that tetrabutylammonium bromide:pyruvate enhanced the destruction of the cell wall structure, resulting in the release of more DMY. Furthermore, different macroporous resins were selected for the separation of DMY for the DES-OS extract, and it was found that the DM301 resin had the ideal recovery performance under optimized dynamic condition. Finally, the product was found to have an inhibitory effect against xanthine oxidase (XO) as a mixed-type competitive inhibitor with IC50 values of (5.79 ± 0.22) × 10−5 mol L−1. The inhibitory mechanisms of DMY on XO were explored by enzyme kinetics, spectroscopy, molecular docking and molecular dynamics analysis approaches, which provided a theoretical basis for the above inhibition assays.
研究领域:
食品科学
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药物化学
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生物化学
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DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
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IF 4.6
Wenhao Wang,Guoying Tan,Qinggui Lei,Jing Chen,Zhengming Qian
A rapid and environmentally friendly quantification method for myricetin and dihydromyricetin in vine tea was developed using direct infusion–tandem mass spectrometry (DI–MS/MS). These compounds are vital functional components known for their anti-inflammatory and antioxidant properties, making them significant for health promotion. DI–MS/MS enhances analytical efficiency by eliminating the need for chromatographic separation, significantly reducing analysis time and solvent consumption compared to previous methods like liquid chromatography–tandem mass spectrometry. This method was validated for accuracy, precision, and stability, ensuring reliable results across various samples. By providing a more sustainable and rapid method for the quality control of vine tea products, this method contributes significantly to the industrial development of vine tea as a functional food product, thereby supporting consumer safety and health benefits in the growing market for herbal teas and natural health products.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2023
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IF 6
Qianqian Li,Bei Li,Chaoyang Zhang,Xiaoqian Zhou,Wei Liu,Yu Mi,Zuorong Xie,Yi Li,Jianxun Li
It is an urgent requirement to explore the key aroma of vine tea to provide the guidance for tea quality evaluation. In this study, three levels of vine tea of bud tip, tender leaf, and aged leaf were characterized by headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME/GC-MS). The relative odor activity value (rOAV) calculation coupled with gas chromatography-olfactometry (GC-O) technology were implemented to seek the aroma-active compounds. Afterwards, the chemometrics methods were employed to explore the critical volatile compounds on the basis of S-line, variable importance for projection (VIP), jack-knifing confidence interval, p values (<0.05), and fold change (FC). As a result, five aroma compounds of ethyl acetate, (E,E)-2,4-heptadienal, geraniol, hexanal, and geranyl acetone were considered as key markers by taking an overall consideration of rOAV, GC-O and chemometrics methods. Finally, the mathematical equation of the vine tea grade with respect to the key aroma was established. The ethyl acetate and geraniol were positively related with the vine tea level evaluation, whereas the hexanal, geranyl acetone, and (E,E)-2,4-heptadienal were negatively correlated with the tea grade, among which the hexanal played a significant role on tea level assessment. This study provides compelling evidence for the vine tea quality evaluation in the perspective of key aroma compounds.
研究领域:
食品科学
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茶叶研究
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Huijun Wang,Jianxun Li,Chaoyang Zhang,Baoshan Sun,Yawen Zhou,Yanyan Zhou,Haiyu Zhao
Plucking position and harvest time influence the metabolites of vine tea which are closely related to the taste quality and antioxidant activity. In this study, 75 vine tea samples produced by three plucking positions and harvested in five months were analyzed by untargeted metabolomics, and in vitro and in vivo antioxidant activities of different vine teas were evaluated. 37 compounds were tentatively identified through various methods. Compared with the high-concentration results, VIP scores of the major components in the low-concentration model were much higher and were filtered as characteristic markers. Chemometrics analysis showed that plucking position had a stronger influence on vine tea quality than harvest time. 16 identified metabolites, including flavonoids, organic acids and catechins, were found to be responsible for the recognition of the plucking position. Vine tea infusion made of young green leaves exhibited higher scavenge radical activity in vitro , and tender tip leaves exhibited higher antioxidant activities in vivo zebrafish, which may be due to the presence of a higher concentration of flavonoids in their phytochemical profile. This research is anticipated to provide useful enlightenment for the effective utilization of vine tea resources.
研究领域:
植物学
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药理学
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食品科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2024
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IF 6.5
Shunyao Qi,Tiexin Zeng,Peiling Wu,Le Sun,Zhengqi Dong,Lijia Xu,Peigen Xiao
The bitter and astringent taste and miscellaneous smell of vine tea prevent its further development. In this study, we used a processing technology that mimics yellow tea to improve the flavor of vine tea and revealed its internal reasons through metabolomics. Sensory evaluation showed the yellowing process for 6–12 h reduced the bitterness and astringency significantly, and enriched the aroma. The improvement of taste was mainly related to the down-regulation of anthocyanins (54.83–97.38%), the hydrolysis of gallated catechins (34.80–47.81%) and flavonol glycosides (18.56–44.96%), and the subsequent accumulation of d -glucose (33.68–78.04%) and gallic acid (220.96–252.09%). For aroma, increase of total volatile metabolite content (23.88–25.44%) and key compounds like geraniol (239.32–275.21%) induced the changes. These results identified the positive effects of yellowing process on improvements in vine tea flavor and the key compounds that contribute to these changes.
研究领域:
食品科学
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代谢组学
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茶叶加工技术
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2024
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IF 5.1
Siyu Du,Xin Chen,Rumeng Ren,Li Li,Baohui Zhang,Qi Wang,Yan Meng,Zhenpeng Qiu,Guihong Wang,Guohua Zheng,Junjie Hu
Nonalcoholic fatty liver disease (NAFLD) is the main reason for chronic liver diseases and malignancies. Currently, there is a lack of approved drugs for the prevention or treatment of NAFLD. Vine tea (Ampelopsis grossedentata) has been used as a traditional Chinese beverage for centuries. Vine tea carries out several biological activities including the regulation of plasma lipids and blood glucose, hepato-protective function, and anti-tumor activity and contains the highest content of flavonoids. However, the underlying mechanisms of total flavonoids from vine tea (TF) in the attenuation of NAFLD remain unclear. Therefore, we investigated the interventions and mechanisms of TF in mice with NAFLD using an integrated analysis of network pharmacology, lipidomics, and transcriptomics. Staining and biochemical tests revealed a significant increase in AKT-overexpression-induced (abbreviated as AKT-induced) NAFLD in mice. Lipid accumulation in hepatic intracellular vacuoles was alleviated after TF treatment. In addition, TF reduced the hepatic and serum triglyceride levels in mice with AKT-induced NAFLD. Lipidomics results showed 32 differential lipids in the liver, mainly including triglycerides (TG), diglycerides (DG), phosphatidylcholine (PC), and phosphatidylethanolamine (PE). Transcriptomic analysis revealed that 314 differentially expressed genes were commonly upregulated in the AKT group and downregulated in the TF group. The differential regulation of lipids by the genes Pparg, Scd1, Chpt1, Dgkz, and Pla2g12b was further revealed by network enrichment analysis and confirmed by RT-qPCR. Furthermore, we used immunohistochemistry (IHC) to detect changes in the protein levels of the key proteins PPARγ and SCD1. In summary, TF can improve hepatic steatosis by targeting the PPAR signaling pathway, thereby reducing de novo fatty acid synthesis and modulating the glycerophospholipid metabolism.
研究领域:
药理学
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分子生物学
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生物信息学
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肝病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
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IF 8.8
Jingjing Chen,Peipei Li,Yixiu Han,Yuchen Li,Yingxiang Du
Highly photoluminescent N -doped carbon quantum dots ( N -CDs) which the quantum yield reached 63% were prepared through hydrothermal treatment. The obtained N -CDs displayed a uniform distribution of particle size, superior stability in high-salt conditions, and excellent sensitivity. A green fluorescence probe based on N -CDs was constructed for ultrasensitive determination of myricetin in vine tea on account of the static quenching. The N -CDs presented excellent linear fluorescence response in the concentration range of 0.2–40 μM and 56–112 μM and with a low detection limit of 56 nM. Additionally, the practicability of the probe was verified in spiked vine tea sample, and the satisfactory recoveries of myricetin varied from 98.8% to 101.2%, with relative standard deviations in the range of 1.52%-3.48%. It is the first time to employ N -CDs without any material modification as a fluorescence sensor to detect myricetin, which is a promising approach to expand the path for myricetin screening.
研究领域:
分析化学
·
纳米技术
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荧光光谱
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药物分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 9
·2100892
·
IF 6.575
Ying Li,Haiming Hu,Huabing Yang,Aizhen Lin,Hui Xia,Xue Cheng,Mingwang Kong,Hongtao Liu
Scope Vine tea ( Ampelopsis grossedentata ), a traditional Chinese tea, has displayed various biological activities. The authors aim to investigate the effect of Vine Tea ( Ampelopsis grossedentata ) extract (VTE) on carbon tetrachlorid (CCl 4 )induced acute liver injury (ALI) in mice and to explore the underlying role of gut microbiota during the treatment. Methods and Results C57BL/6J mice injected with CCl 4 are treated with VTE for 6 weeks. By using H&E staining, immunofluorescence staining, quantitative real-time (qRT)-PCR, and western blot, it is shown that VTE treatment significantly ameliorates hepatocyte necrosis, alleviates the mRNA levels of toll-like receptor 4 ( Tlr4) , interleukin (Il)-6 , inducible nitric oxide synthase (iNOS) , acetyl-CoA carboxylase 1 (Acc1) , and increases the mRNA levels of peroxisome proliferator-activated receptor gamma (Ppar-γ) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (Hmg-coar) compared to the CCl 4 group. Also, VTE abrogates the decreased mRNA expressions of zonula occludens-1 (Zo-1) , Occludin , and Mucin1 in colon tissues. Using microbial 16S rDNA sequencing, VTE treatment significantly downregulates the abundances of some harmful intestinal bacteria like Helicobacter and Oscillibacter . In contrast, VTE upregulates the contents of several beneficial bacteria, such as Ruminococcaceae_UCG-014 and Eubacterium_fissicatena_group . Further, VTE fails to improve ALI in the mice with gut microbiota depletion using antibiotic treatment. Conclusions The studies suggest that VTE exhibits a protective effect against CCl 4 -induced ALI in mice by alleviating hepatic inflammation, suppressing intestinal epithelial barrier injury, and restoring gut microbiota dysbiosis.
研究领域:
药理学
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微生物学
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肝病学
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免疫学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）