ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Huiting Yang,Yujia Yao,Wei Chen,Xiaohong Gu,Huihui Chen,Qingfei Zeng,Zhigang Mao,Tao Xiang
Cyanobacterial blooms can generate various toxic metabolites in freshwater, and pose serious threats to drinking water safety and human health. Although microcystins (MCs) have been detected in many freshwater ecosystems in China, little is known about the other cyanotoxins. An investigation of six eutrophic lakes (i.e. Hulun Lake, Wuliangsuhai Lake, Chaohu Lake, Taihu Lake, Xingyun Lake, and Dianchi Lake) in different geographical locations of China was performed during the summer of 2022 to determine the occurrence of various cyanotoxins (i.e. anatoxin-a (ATX), cylindrospermopsin (CYN), and MCs) in water column and their possible risks, and to evaluate the related environmental factors. MCs levels in sediment of these lakes were also investigated. MCs were the primary cyanotoxins in the water column of investigated lakes. The mean MCs contents in water column of Hulun Lake, Wuliangsuhai Lake, Chaohu Lake, Taihu Lake, Xingyun Lake, and Dianchi Lake were 3.61, 0.13, 3.60, 2.18, 0.57, and 2.56 μg/L, respectively. The total MCs levels in water column exceeded 1 μg/L in some areas of these lakes except Wuliangsuhai Lake. Replete nitrogen and/or phosphorus levels seemed to be related to MCs production. ATX can be detected in these lakes except Xingyun Lake at ng/L levels. CYN can be detected in all lakes at ng/L levels. However, the levels of ATX and CYN appear to be not significantly associated with environmental factors. MCs and CYN can pose a high or moderate risk for humans and aquatic organisms in some areas of these lakes, while ATX can pose a low or no risk for humans and aquatic organisms in most areas of these lakes. MCs can also be detected in sediment of all lakes at ng/g levels. This research emphasizes the necessity for long-term monitoring of different cyanotoxins in eutrophic lakes, and the implementation of nutrient control and management strategies.
研究领域:
生态学
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环境科学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Bai-Lin Liu,Yan-Wen Li,Xi-Ying Tu,Peng-Fei Yu,Lei Xiang,Hai-Ming Zhao,Nai-Xian Feng,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Ming Hung Wong
Microcystins (MCs) are hepatotoxic heptapeptides identified in cyanobacterial bloom-impacted waters and soils. However, their environmental fate in soils is poorly understood, preventing reliable site assessment. This study aims to clarify the variant-specific adsorption, desorption, and dissipation of MC-LR and MC-RR in agricultural soils. Results revealed that their adsorption isotherms followed the Freundlich model (R2 ≥ 0.96), exhibiting a higher nonlinear trend and lower adsorption capacity for MC-LR than for MC-RR. The soils had low desorption rates of 8.14–21.06% and 3.06–34.04%, respectively, following a 24 h desorption cycle. Pairwise comparison indicated that soil pH and clay played key roles in MC-LR adsorption and desorption, while organic matter and cation exchange capacity played key roles in those of MC-RR. MC-LR dissipation half-lives in soils were 27.18–42.52 days, compared with 35.19–43.87 days for MC-RR. Specifically, an appreciable decrease in MC concentration in sterile soils suggested the significant role of abiotic degradation. This study demonstrates that the minor structural changes in MCs might have major effects on their environmental fates in agricultural soil and indicates that the toxic effects of MCs should be of high concern due to high adsorption, low desorption, and slow dissipation.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Catalysis
·
2022
·
IF 13.7
Yan Shi,Jingzhi Li,Di Huang,Xiawei Wang,Yingping Huang,Chuncheng Chen,Ruiping Li
The oxygen-vacancy (OV)-rich BiOBr exhibits higher photocatalytic activity than that of the OV-deficient one. The interaction of the target substrate with the surface OV sites should be essential for the high activity, but it is not well understood so far. Here, we used a cyanotoxin cylindrospermopsin (CYN) as a model substrate to investigate the specific adsorption of pollutants with highly hydrophilic groups on OV sites of BiOBr. We found that the adsorption of CYN on BiOBr with rich OVs is much stronger than the adsorption on BiOBr with poor OVs, and the BiOBr with rich OVs also exhibits a higher degradation rate of CYN. Various experiments indicated that the strong adsorption of CYN on OV-rich BiOBr originates from the strong interaction between the highly hydrophilic sulfate group of CYN and OV sites. Density functional theory calculations suggest that, on OV sites, Brönsted acid sites (OHB) are formed through the dissociative adsorption of water molecules. The combining effect of the hydrogen bonding with these OHB and the coordination with the Lewis acid Bi sites make the adsorption of the sulfate group quite strong. Such a strong interaction can well explain the rapid degradation of CYN, which proceeds through direct oxidation by h+ or by the OHB-derived •OH. Based on ultra-performance liquid chromatography–tandem mass spectrometry analysis, the CYN degradation begins via hydroxylation at the C5–C6 double bond in the uracil ring by •OH, which is further degraded by two pathways. This work provides a deeper understanding of the critical roles of OVs in the processes of adsorption and photocatalysis for organic pollutant removal.
研究领域:
环境科学
·
材料科学
·
毒理学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Qing Yang,Yongqiang Liu,Lihong Wang,Qing Zhou,Mengzhu Cheng,Jiahong Zhou,Xiaohua Huang
Aggravating the pollution of microcystins (MCs) in freshwater environments is detrimental to aquatic living organisms and humans, and thus threatens the stability of ecosystems. Some environmental factors have been verified to promote the production of MCs in Microcystis aeruginosa , thereby aggravating the pollution of MCs. However, the effects of cerium (Ce), the most abundant rare earth element in global water environments, on the production of MCs in M. aeruginosa are unknown. Here, Lake Taihu water was selected as a representative of freshwater environments. By using interdisciplinary methods, it was found that: (1) the exposure level of Ce [Ce(III) and Ce(IV)] in Lake Taihu water is in the range of 0.271–0.282 μg/L; (2) Ce exposure in Lake Taihu water promoted the contents of three main MCs (MC-LR, MC-LW and MC-YR) in M. aeruginosa and water; (3) a cellular mechanism of Ce promoting the production of MCs in M. aeruginosa in Lake Taihu water was suggested: Ce enhanced endocytosis in cells of M. aeruginosa to promote the essential element uptake by M. aeruginosa for MC synthesis. Thus, Ce exposure in Lake Taihu water aggravates the pollution of MCs via enhancing endocytosis in cells of M. aeruginosa . The results provide reference for assessing the environmental risk of Ce in water environments, investigating the mechanism of the pollution of MCs induced by environmental factors, and developing strategies aimed at preventing and controlling the pollution of MCs.
研究领域:
环境科学
·
生物学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY
·
2014
·Vol. 24
·期 1
·19-28
·
IF 2.5
Zhang Dawen,Liao Qiegen,Zhang Li,Wang Donggen,Luo Linguang,Chen Yuwei,Zhong Jiayou,Liu Jutao
Occurrence and spatial distributions of microcystins (MCs; MC-RR, -YR, -LR, -LA, -LF, -LW) in Poyang Lake were studied during the period from July 6 to July 18, 2012, by using ultra-high-performance liquid chromatography–electrospray ionization tandem triple quadrupole/mass spectrometry (UPLC-MS/MS). MC-RR was the most dominant variant (94.70 and 84.73 % for intracellular (cellular MCs) and extracellular (dissolved MCs) MCs, respectively) in Poyang Lake, followed by MC-LR (4.65 and 13.17 %, respectively), MC-YR (0.8 and 2.63 %, respectively), and MC-LA (0.02 and 0.00 %), while MC-LW and MC-LF were not detected. Total MCs concentrations (intracellular +extracellular MCs) ranged between 0.0036 and 7.97 μg/L, with an average of 0.79 μg/L, and only two sampling stations with the total MCs concentrations exceeded the drinking water guideline level of 1 μg/L for MC-LR proposed by World Health Organization. The overall spatial pattern of intracellular and extracellular MCs in Poyang Lake demonstrates decreasing trends from east to west, and the south part higher than the north part. Intracellular MCs content was negatively correlated with total nitrogen (r = −0.34, p < 0.01) and NO 3 (r = −0.35, p < 0.01), while no significant correlation was found between intracellular MCs concentration and total phosphorus, NH 4 , and NO 2 ( p > 0.05), suggesting that NO 3 might be a regulating factor for MCs production in Poyang Lake. In addition, intracellular MCs concentrations were positively correlated with wind speed, Microcystis and Cyanobacteria biomass (r = 0.34–0.51, p < 0.05), indicating that wind speed plays an important role in the spatial distributions of MCs, and NO 3 , toxic cyanobacteria (mainly Microcystis ), and wind speed seem to be the important forcing factors driving MCs spatial distributions in Poyang Lake.
研究领域:
环境科学
·
水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 37
·23002-23009
·
IF 3.361
Peng Jin,Kai Yang,Ruining Bai,Mei Chen,Shilin Yang,Kebo Fu,Jieli He
Microcystin-LR (MC-LR) generated by cyanobacteria is a kind of potent hepatotoxin, which poses a considerable threat to human health. In the research field of MC-LR removal, the quantitative analysis in a wide concentration range of samples is inevitable. In this paper, we presented the pseudo united use of an Ultra Performance Liquid Chromatography Mass Spectrometry (UPLC-MS) and High Performance Liquid Chromatography system with a Variable Wavelength Ultraviolet Detector (HPLC-VWD) approach to detect MC-LR. The UPLC-MS system was applied to determine MC-LR in trace concentration because of its high sensitivity. However, it is generally believed that the determination of high concentration samples by UPLC-MS will cause problems such as inaccurate quantification and contamination of ion sources. In consequence, the HPLC-VWD was employed to determine the high concentration of MC-LR. The sensitivity, precision and accuracy of the two methods were compared in detail. The linear ranges of UPLC-MS and HPLC-VWD methods were from 0.08 to 10 μg L−1 and 1 to 5000 μg L−1, respectively. The detection and quantification limits of UPLC-MS were 0.03–0.05 μg L−1 and 0.08 μg L−1, and the corresponding two values of HPLC-VWD were 0.6 and 1.0 μg L−1. The recoveries of UPLC-MS and HPLC-VWD were 88.5–106.7% and 98.7–101.6%, with the relative standard deviations of 3.72–5.45% and 0.38–1.69%, respectively. The potential adsorption properties of MC-LR on filter membranes with diverse materials and pore sizes were evaluated and the negative results were obtained. The detection of MC-LR by UPLC-MS was free from matrix effects. The presented UPLC-MS and HPLC-VWD methods were used to analyze the water samples from Erhai Lake, which is located in Dali, Yunnan, China. The results of UPLC-MS analysis indicated that the MC-LR was only identified in water samples of Shuanglang Bay and Xier River, with concentrations of 0.120 and 0.303 μg L−1, whereas MC-LR was not detected by HPLC-VWD.
研究领域:
环境科学
·
毒理学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2022
·Vol. 32
·期 2
·505-519
·
IF 2.525
Liu Yipeng,Li Bingyan,Zhang Huixia,Liu Yong,Xie Ping
Microcystin-LR (MC-LR) is widely distributed in natural lakes and could strongly inhibit protein phosphatase activity; it is also a potent liver tumor promoter. Over the last two decades, tremendous efforts have been devoted to enhance the detection of MC-LR in water samples. However, the traditional method is complex and costly, and achieving the fast, sensitive, and accurate determination of MC-LR in the cells and natural lakes by using fluorescence signal changes is fairly difficult. Our work explores novel fluorescent probes that are capable of concurrently analyzing and detecting MC-LR in the cells and water. In this study, we introduce, for the first time, 5-AF and 6-AF as small-molecule fluorescent probes suitable for MC-LR detection in the cells and water samples based on fluorescence signal changes. We titrated 5-AF and 6-AF with MC-LR in pure water, scanned the fluorescence of the sample, and then obtained the equation the fluorescence intensity versus MC-LR concentration curve. MC-LR in lake water samples was crudely purified, and then 5-AF was added to measure its fluorescence peak. The fluorescence intensity of 5-AF is significantly enhanced with increasing MC-LR concentration. This enhancement trend is stable and could be mathematically modeled. We also comprehensively analyzed the mechanism and recognition principle of the probe response to MC-LR in natural lake water. Moreover, we believe that 5-AF may be capable of detecting exogenous MC-LR in cells. The results of this study reveal that these unique fluorescent probes may be applied to construct near-infrared fluorescent probes that could detect MC-LR levels in vivo. Graphical Abstract
研究领域:
环境科学
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Lele Wang,Rui Ma,Wenjing Wang,Ling Zhang,Jiao Li,Jianan Sun,Sai Wang,Xiangzhao Mao
Highly efficient detection of group targets is of great significance. Okadaic acid (OA), dinophysistoxin-1 (DTX-1), and dinophysistoxin-2 (DTX-2) are the main diarrhetic shellfish poisons (DSPs) and have similar structures. As essential auxiliary tools, facile methods for simultaneous screening of these three DSPs are urgently needed. In this study, we report an aptamer-based platform which integrates pretreatment and quantitation for preliminary and simultaneous screening of the three DPSs. With computational study-assisted post-selection strategies, a truncated aptamer and a pair of split aptamers were generated with group-specific and high affinity towards the DSPs. The truncated aptamer was adopted to prepare an aptamer affinity column (AAC) for enrichment and purification of DSPs in real samples. The split aptamers were combined with G-quadruplex-hemin nanozyme to fabricate a label-free colorimetric aptasensor for quantitation. The prepared AAC showed good enrichment capacity. The developed colorimetric aptasensor provided high sensitivity, with limit of detection of 0.7011 nM, 14.27 nM, and 5.374 nM towards OA, DTX-1, and DTX-2, respectively, as well as high selectivity and accuracy. UPLC-MS was then only needed to analyze the suspicious positive samples, and highly efficient detection was achieved. This robust platform could be referred for preliminary screening of other group analytes.
研究领域:
分析化学
·
毒理学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Gongduan Fan,Zhong Chen,Shiping Gu,Banghao Du,Lihui Wang
Cyanobacterial harmful algal blooms (CyanoHABs) and the release of cyanotoxins have posed adverse impacts to aquatic system and human health. In this study, a novel self-floating Ag/ [email protected] 3 (ALFO) photocatalytic hydrogel was prepared via freeze-thaw method for removal of Microcystis aeruginosa ( M. aeruginosa ). The ALFO hydrogel performed an excellent photocatalytic activity with a 99.4% removal efficiency of chlorophyll a within 4 h. It can still remove above 95% chlorophyll a after six consecutive recycles. Besides it has also shown excellent mechanical strength and elasticity, which can ensure its use in practical applications. The mechanisms of M. aeruginosa inactivation are attributed to •O 2 − and •OH generated by the ALFO hydrogel under visible light radiation . In addition, •O 2 − and •OH can further oxidative degrade and even mineralize the leaked algae organic matter, avoiding the recurrence of CyanoHABs. What's more, the ALFO hydrogel owns good photocatalytic degradation performance for microcystins-LR (MC-LR) with a 97% removal efficiency within 90 min. A possible photocatalytic degradation pathway of MC-LR was proposed through the identification of the intermediate products during the photocatalytic reaction, which confirmed the reduction of MC-LR toxicity. This work develops recyclable a self-floating ALFO hydrogel to simultaneously inactivate M. aeruginosa and degrade MC-LR, providing a prospective method for governing and controlling CyanoHABs in practical application.
研究领域:
环境科学
·
水处理
·
光催化
·
毒理学
·
水生生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Haishuo Wang,Xinke Tian,Zhichun Li,Jie Zhang,Shiyin Li,Heyong Huang
Lake eutrophication leads to the excessive proliferation of cyanobacteria in aquatic environments, resulting in microcystin-LR (MCLR) concentrations exceeding regulatory limits. The associated environmental risks and health concerns attract widespread attention. As one of the most active chemical substances in aquatic environments, dissolved organic matter (DOM) plays a critical role in the phototransformation of MCLR. In environments where Fe(II) and Mn(II) coexist, the phototransformation process of MCLR may be influenced. Among fulvic acid (FA), humic acid (HA), cyanobacteria-derived DOM (CDOM), and reed leaf-derived DOM (RDOM), FA has the highest apparent reaction rate constant for MCLR at 0.03567 h -1 . Using isopropanol, furfuryl alcohol, and sorbic acid as quenchers, the contributions of different photochemically produced reactive intermediates(PPRIs) to MCLR photodegradation follow the order: 3 DOM⁎ > ·OH > 1 O 2 . As pH increases, changes in the conformation and charge distribution of DOM and MCLR suppress MCLR photodegradation. The addition of Fe(II) or Mn(II) enhances the photodegradation of MCLR by DOM. Electron paramagnetic resonance tests and radical quenching experiments indicate that the introduction of Fe(II)/Mn(II) increases ·OH production. The enhanced ·OH promotes MCLR photodegradation more effectively than the suppression caused by Fe(II)/Mn(II) quenching of ³DOM⁎. Product analysis reveals that demethylation, hydroxylation, decarbonylation, decarboxylation, denitration, and bond cleavage are the main pathways of MCLR photodegradation. These findings provide valuable insights into the migration and transformation of MCLR in complex environments, contributing to a deeper understanding of the fate of pollutants in natural water systems, particularly in the presence of metal ions.
研究领域:
环境科学
·
水生生态学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 10
·2398
·
IF 4.35
Xiya Zhang,Mingyue Ding,Chensi Zhang,Yexuan Mao,Youyi Wang,Peipei Li,Haiyang Jiang,Zhanhui Wang,Xuezhi Yu
The consumption of shellfish contaminated with brevetoxins, a family of ladder-frame polyether toxins formed during blooms of the marine dinoflagellateKarenia brevis, can cause neurotoxic poisoning, leading to gastroenteritis and neurotoxic effects. To rapidly monitor brevetoxin levels in oysters, we generated a broad-spectrum antibody against brevetoxin 2 (PbTx-2), 1 (PbTx-1), and 3 (PbTx-3) and developed a rapid indirect competitive enzyme-linked immunosorbent assay (icELISA). PbTx-2 was reacted with carboxymethoxylamine hemihydrochloride (CMO) to generate a PbTx-2-CMO hapten and reacted with succinic anhydride (HS) to generate the PbTx-2-HS hapten. These haptens were conjugated to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA) to prepare immunogen and coating antigen reagents, respectively, using the active ester method. After immunization and cell fusion, a broad-spectrum monoclonal antibody (mAb) termed mAb 1D3 was prepared. The 50% inhibitory concentration (IC50) values of the icELISA for PbTx-2, PbTx-1, and PbTx-3 were 60.71, 52.61, and 51.83 μg/kg, respectively. Based on the broad-spectrum mAb 1D3, an icELISA was developed to determine brevetoxin levels. Using this approach, the limit of detection (LOD) for brevetoxin was 124.22 μg/kg and recoveries ranged between 89.08% and 115.00%, with a coefficient of variation below 4.25% in oyster samples. These results suggest that our icELISA is a useful tool for the rapid monitoring of brevetoxins in oyster samples.Keywords:brevetoxins;monoclonal antibody;enzyme-linked immunosorbent assay
研究领域:
海洋生物学
·
免疫学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2023
·
IF 22.1
Panpan Gao,Yunyi He,Shihuan Lu,Mengfan He,Zhiqun Liu,Yang Deng,Zhiquan Liu,Ting Xu,Hangjun Zhang
The cyanobacterial blooms induced water safety problems have attracted tremendous concerns due to the adverse influences on aqueous ecosystem and human health. This study developed a novel perovskite-type catalyst for heterogeneous peroxymonosulfate (PMS) activation as an emergency measure to remove harmful algae. The results indicated that significant removal efficiency of Microcystis aeruginosa cells (97%) and chlorophyll-a (100%) was achieved within 20 min in La 2 CuO 4 /PMS system without significant release of dissolved organic carbon and metals in water. • OH, SO 4 •- , 1 O 2 and • O 2 - generated were all responsible for algae inactivation and subsequent algal organic matters removal. Moreover, the heterogeneous catalytic mechanism was proposed that La portion in La 2 CuO 4 favored the cells destabilization by increasing zeta potential of algae suspension, while variable Cu portion facilitated the electron transfer to improve the yields of • OH and SO 4 •- . This study demonstrates that the new catalytic PMS process promises viable and effective control of harmful algal bloom impacts in water treatment.
研究领域:
环境科学
·
水处理技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）