GLYCOBIOLOGY
·
2023
·
IF 4.3
Li Chen,Tang Tiancheng,Jiang Jinju,Yao Zhong,Zhu Benwei
Ulva is globally distributed specie and has a high economic value. Ulvan is one of the main active substances in Ulva, which has a variety of biological properties. Ulvan lyase degrades ulvan through a β-elimination mechanism which cleaves the β-glycosidic bond between Rha3S and GlcA or IdoA. The complex monosaccharide composition of ulvan makes it promising for use in food and pharmaceutical applications. This thesis explores a putative ulvan lyase from Alteromonas sp. KUL_42. We expressed and purified the protein, performed a series of characterizations and signal peptide had been removed. The results showed that the protein molecular weight of ULA-2 was 53.97 kDa, and it had the highest catalytic activity at 45 °C and pH 8.0 in Tris–HCl buffer. The Km and Vmax values were 2.24 mg·mL−1 and 2.048 μmol·min−1·mL−1, respectively. The activity of ULA-2 was able to maintain more than 80% at 20 ~ 30 °C. ESI-MS analysis showed that the primary end-products were mainly disaccharides to tetrasaccharides. The study of ULA-2 enriches the ulvan lyase library, promotes the development and high-value utilization of Ulva resources, and facilitates further research applications of ulvan lyase in ulva oligosaccharides.
研究领域:
生物化学
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生物技术
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海洋生物学
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食品科学
·
药学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2025
·
IF 4
Kit-Leong Cheong,Keying Liu,Suresh Veeraperumal,Gowsika Jaikumar,Sathuvan Malairaj,Xiaofei Liu,Xuejing Jia,Peiying Zheng,Hengyi Jiang,Ruixu Cai,Saiyi Zhong,Udayakumar Veerabagu
Laminarin, a polysaccharide derived from brown algae, has gained attention for its potential biological activities. However, its application in cosmetic formulations is limited due to its functional properties. To enhance its bioavailability and performance, laminarin was modified through esterification to form laminarin butyl esters (LBE). This study aimed to evaluate the antioxidant, skin-whitening, and anti-wrinkle properties of LBE and assess its potential as a novel ingredient in skincare products. Antioxidant activity was evaluated using in vitro assays, including DPPH and ABTS radical scavenging, both of which demonstrated significant free radical neutralizing capacity. Skin-whitening and anti-wrinkle activities were assessed by measuring the inhibition of tyrosinase and elastase. The results indicated that LBE exhibited significantly higher inhibitory effects than native laminarin, with tyrosinase inhibition reaching 26.7 % (compared to 4.7 % for laminarin) and elastase inhibition at 45.8 % (versus 18.5 % for laminarin). Additionally, LBE showed stronger anti-glycation activity, as it effectively inhibited Amadori products, dicarbonyl compounds, and advanced glycation end-products. These findings provide valuable insights into the modification of laminarin and its potential application in cosmetics, offering a foundation for the development of more effective, bioactive ingredients in the beauty industry.
研究领域:
化妆品科学
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生物化学
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产品关联
DOI
高影响因子
Marine Drugs
·
2022
·Vol. 20
·期 8
·533
·
IF 6.085
Juanjuan Wang,Zebin Liu,Xiaowei Pan,Ning Wang,Legong Li,Yuguang Du,Jianjun Li,Mei Li
Fucoidans represent a type of polyanionic fucose-containing sulfated polysaccharides (FCSPs) that are cleaved by fucoidan-degrading enzymes, producing low-molecular-weight fucoidans with multiple biological activities suitable for pharmacological use. Most of the reported fucoidan-degrading enzymes are glycoside hydrolases, which have been well studied for their structures and catalytic mechanisms. Little is known, however, about the rarer fucoidan lyases, primarily due to the lack of structural information. FdlA fromFlavobacteriumsp. SA-0082 is an endo-type fucoidan-degrading enzyme that cleaves the sulfated fuco-glucuronomannan (SFGM) through a lytic mechanism. Here, we report nine crystal structures of the catalytic N-terminal domain of FdlA (FdlA-NTD), in both its wild type (WT) and mutant forms, at resolutions ranging from 1.30 to 2.25 Å. We show that the FdlA-NTD adopts a right-handed parallel β-helix fold, and possesses a substrate binding site composed of a long groove and a unique alkaline pocket. Our structural, biochemical, and enzymological analyses strongly suggest that FdlA-NTD utilizes catalytic residues different from other β-helix polysaccharide lyases, potentially representing a novel polysaccharide lyase family.Keywords:fucoidan lyase;polysaccharides;crystal structure;catalytic mechanism
研究领域:
生物化学
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结构生物学
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DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Qianni Chen,Lianzhu Lin,Mouming Zhao
Fucoidan possesses a variety of biological activities beneficial for human wellness. It is a good candidate for ameliorating obesity and diabetes. However, high-purity fucoidan products are limited due to the co-extraction of impurities i.e. alginate, protein and small molecular substances. In this study, the optimal preparation strategy for high-purity fucoidan from a template brown alga ( Saccharina japonica ) was screened out by the exploration of key and auxiliary steps. The key step was confirmed as moderate-temperature acid extraction and the auxiliary steps were fixed on ethanol refluxing and ball milling pretreatment. The fucose content of S. japonica fucoidan increased by 7.15 times and the sulfate group content increased by 4.87 times compared with those of fucoidans prepared by classic high-temperature acid extraction. Ball milling-assisted moderate-temperature acid extraction performed great effectiveness and universality in the extraction of high-purity fucoidans derived from four brown algae including S. japonica , Undaria pinnatifida, Sargassum fusiforme, and Fucus vesiculosus . Glycolipid-lowering activities of four fucoidans were confirmed by their interactions with intestinal bile acid, cholesterol micellar, glucose and probiotics including butyrate-producing bacteria, Bacteroides , Bifidobacterium , and Lactobacillus . Our study provided an efficient and universal extraction process for high-purity fucoidan with glycolipid-lowering activity from typical brown algae.
研究领域:
海洋生物学
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食品科学
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生物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 9
·4488
·
IF 6.208
Li Gao,Chen Xu,Xuelin Tao,Zhichuang Zuo,Zimo Ning,Linghui Wang,Na Gao,Jinhua Zhao
Fucan sulfate I (FSI) from the sea cucumberHolothuria fuscopunctatawas purified and its structure was clarified based on a bottom-up strategy. The unambiguous structures of a series of oligosaccharides including disaccharides, trisaccharides, and tetrasaccharides, which were released from mild acid hydrolysis of FSI, were identified by one-dimensional (1D)/two-dimensional (2D) nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. All the glycosidic bonds in these oligosaccharides were presented as α1,3 linkages confirmed by correlated signals from their1H-1H ROESY and1H-13C HMBC spectra. The structural sequence of these oligosaccharides formed by Fuc2S4S, Fuc2S, and non-sulfated ones (Fuc0S), along with the general structural information of FSI, indicated that the structure of FSI could be elucidated as: [-L-Fuc2S4S-α1,3-L-Fuc(2S)-α1,3-L-Fuc2S-α1,3-L-Fuc0S-α1,3-1-]n. Moreover, the L-Fuc0S-α1,3-L-Fuc2S4Slinkage in FSI was susceptible to be cleaved by mild acid hydrolysis. The antioxidant activity assays in vitro showed that FSI and the depolymerized product (dFSI′) had potent activities for superoxide radical scavenging activity with IC50of 65.71 and 83.72 μg/mL, respectively, while there was no scavenging effect on DPPH, hydroxyl and ABTS radicals.Keywords:fucan sulfate;mild acid hydrolysis;oligosaccharides;structure;antioxidant activity
研究领域:
海洋生物学
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Food Packaging and Shelf Life
·
2025
·
IF 10.6
Linlin Li,Haoran Wang,Yimeng Zhang,Wei Ji,Minmin Chen,Baocai Xu,Hualin Wang
Food packaging combined with active and intelligent properties plays an essential role in minimizing food waste and guaranteeing food quality. Herein, we developed a colorimetric and control-released dual-responsive nanofiber membrane (GF-AE-LI) with gelatin/fucoidan (GA/FU, i.e., GF) as matrix, aloe-emodin (AE) as indicator and limonene (LI) as antibacterial entity by electrospinning. The results exhibited that an appropriate combination of GA and FU based on the electrostatic interaction was conducive to constructing a carrier of GF in response to an alkaline atmosphere. The AE and LI were encapsulated in GF nanofibers with high efficiency and well-kept structure. Moreover, the membrane was capable of pH-controlled release of LI by a non-Fickian diffusion mechanism and strong antibacterial effects against Staphylococcus aureus and Escherichia coli . Furthermore, the membrane can effectively extend the shelf life of chicken breast meat by 2 d, and its pH-colorimetric changes were highly correlated with the spoilage indices (Pearson′s r = 0.9554), demonstrating the potential of GF-AE-LI toward simultaneously freshness monitoring and preservation of food.
研究领域:
食品科学
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材料科学
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生物工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Wenhao Liu,Xiaoye Xu,Wanjing Liu,Xinxin Zeng,Si Shi,Jianxi Zhang,Junjie Tang,Yuanzhao Li,Jie Pang,Chunhua Wu
Fucoxanthin (FX), a marine-origin carotenoid, possesses various physiological activities. However, FX has instability and low water solubility. Encapsulation using nanoparticles effectively addresses these challenges. Nanoparticles loaded with FX were fabricated using a pH-driven method, with pea protein isolate (PPI) and fucoidan (FUC) serving as the raw materials. The optimal nanoparticles were prepared at pH = 7.0 with a PPI:FUC = 1:3, yielding a particle size of 166.60 ± 0.55 nm and a zeta potential of −40.88 ± 0.68 mV. The formation of FX@PPI/FUC nanoparticles were primarily driven by hydrogen bonding and hydrophobic interactions. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and fluorescence spectroscopy were used to research structure of nanoparticle and interaction during the formation. The FX@PPI/FUC nanoparticles demonstrated excellent thermal and pH stability in neutral and alkaline environments, effectively released FX and showcased antioxidant properties. Additionally, a W/O/W FX@PPI-FUC Pickering emulsion was formulated, containing 65 % of the oil phase, which exhibited a favorable particle size of 26.5 ± 0.28 μm and a zeta potential of −67.2 ± 0.94 mV. Furthermore, the FX@PPI-FUC Pickering emulsion demonstrated outstanding thermal and storage stability, indicating its potential for application in functional food.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2024
·
IF 11.4
Panpan Li,Jianguo Zhang,Yongheng Shen,Xuefei Feng,Wenlin Jia,Mingkai Liu,Shuang Zhao
To address the problems of unstable efficiency, long treatment period, and high energy consumption during microplastics (MPs) removal by traditional coagulation–flotation technology, a gel coagulation–spontaneous flotation (GCSF) process is proposed that employs laminarin (LA) as the crosslinker and polyaluminum chloride (PAC)/polyaluminum ferric chloride (PAFC) as the coagulant to remove MPs. Herein, the effects of GCSF chemical conditions on microplastic-humic acid composite pollutants (MP-HAs) removal were investigated, and the removal mechanisms were analyzed through theoretical calculations and floc structure characterization. Results showed that an LA to PAC/PAFC ratio of 2.5:1 achieved the highest removal of HA (86 %) and MPs (93 %–99 %) in short coagulation (< 1 min) and spontaneous flotation (< 9 min) period. PAC-LA exhibited strong removal ability for MP-HAs while PAFC-LA induced fast flotation speed. The peak intensity and peak shift in Fourier-transformed infrared and X-ray photo-electron spectra indicated that the removal mechanisms of MPs include hydrogen bond adsorption and the sweeping effect, mainly relying on –OH/–C = O on the MPs surface and entrapment of gel flocs with a high degree of aggregation, respectively. The extended Derjaguin-Landau-Verwey-Overbeek calculation also revealed that interactions between PAC/PAFC-LA and MP-HAs were mainly polar interaction (hydrogen bonding) and intermolecular attraction interaction (Lifshitz-van der Waals force), and the sweep effect was reflected by intermolecular interaction. In addition, density function theory calculations indicated that –OH in LA mainly adsorbs DO through a double hydrogen bond configuration, and the crosslinking ligand FeO 6 /AlO 6 assists in DO absorption by –OH.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Tao Zhang,Ruowei Huang,Xiaoqi Xu,Yaoguang Chang,Changhu Xue
This study was aimed at enhancing the stability of the medium internal phase emulsion by modulating the electrostatic interaction between fucoidan (FUC) and sodium caseinate (NaCS). Firstly, the macro and microstructure of FUC-NaCS electrostatic complexes with the mass ratio of ranged from 0.01: 2 to 4: 2 were examined at pH 3.0. As the concentration of FUC increased, the complexes transformed a soluble state to phase separation and then returned to the homogeneous state, with the turbidity and particle size initially increasing and then decreasing. The zeta potential of the complexes gradually declined, and the zero-point was corresponding to the phase separation region where the ratio of FUC-NaCS was around 0.75: 2. The microstructure by TEM and SEM demonstrated that the NaCS particles were bonded to the FUC cross-linking chain network via electrostatic interaction to form self-assembling complexes. Subsequently, the simulated digestion results suggested that the FUC-NaCS complexes could delay the hydrolysis of NaCS and displayed greater stability in gastric environments. Based on the above results, the FUC-NaCS complexes were utilized to emulsify and stabilize medium chain triglycerides (MCT). The droplets of the FUC-NaCS emulsion at 4: 2 was more dispersed with a smaller diameter, and also exhibited superior stability during stomach digestion and concurrently high digestibility during intestinal digestion, making FUC-NaCS complexes a potential choice for pH-triggered release of emulsions, especially in gastrointestinal environment.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2025
·Vol. 23
·期 2
·64
·
IF 5.4
Xuedong Zhang,Guangwei Yan,Xinming Liu,Jiewen Fu,Xiang Shi,Pei Cao,Yuqian Sun,Shengping Zhong,Jiale Nong,Peiqi Jiang,Yonghong Liu,Baoshun Zhang,Qingxia Yuan,Longyan Zhao
The depolymerized products and oligosaccharide fractions from sea cucumber fucosylated glycosaminoglycans (FGs) are promising anticoagulant candidates, and more novel FG-derived oligosaccharides from low-priced sea cucumbers are expected to be obtained. This study isolated 5−12 oligomers (OF1−OF3) with unusual branches from β-eliminative depolymerized products ofColochirus quadrangularisFG (CqFG). Detailed NMR analyses showed that OF1−OF3 consisted of a chondroitin 4,6-sulfates backbone and some sulfated fucosyl branches (FucS), including monosaccharides (α-l-Fuc2S4S, α-l-Fuc3S, α-l-Fuc4S, α-l-Fuc2S3S4S, and α-l-Fuc2S) and a disaccharide D-Gal3S4S-α1,3-l-Fuc2S4Swith the ratio of ~36:35:10:7:3:9, attached to the C-3 position of β-d-GlcA or its derivatives, such as α-l-Δ4,5GlcA and β-d-GlcA-ol. Unusually, α-l-Fuc3Swas the main FucS branch; no α-l-Fuc3S4Sbranch was found, and α-l-Fuc2S3S4Sand α-l-Fuc2Sbranches were also found in OF1–OF3. The OF2 and OF3 could strongly inhibit the intrinsic and common coagulation pathways. Intrinsic FXase is a target of OF2 and OF3 inhibiting the intrinsic coagulation pathways, and the unusual side chains may increase the intrinsic FXase inhibitory activity. OF2 and OF3 showed negligible bleeding risk, and less bleeding than heparin (HP), low-molecular-weight heparins (LMWHs), and CqFG. These findings support novel FG oligosaccharides with some unusual branches from low-priced sea cucumbers to be prepared as safer anticoagulants.
研究领域:
海洋生物化学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2023
·Vol. 11
·期 10
·3524-3546
·
IF 6.6
Jing-Hong Liu,Jian-Ming Ouyang
Objective: The first objective is to study the synergistic inhibition of calcium oxalate (CaOx) formation by Laminarin polysaccharides (DLP and SDLP, before and after sulfation) and potassium citrate (K3cit) and determine the synergistic protection of renal epithelial cells (HK-2 cells) caused by CaOx crystal damage. The second objective is to explore new ways to prevent and treat kidney stones. Methods: The CaOx crystals regulated by five additives (K3cit group, DLP group, SDLP group, DLP–K3cit synergistic group and SDLP–K3cit synergistic group) were characterized by FT-IR, XRD, SEM, zeta potential, ICP, and TGA. The protective effect of each additive group on HK-2 cells damaged by nano-calcium oxalate monohydrate (nano-COM) was compared by detecting cell viability, the cell reactive oxygen species level, the cell survival rate, and mitochondrial membrane potential. Results: When DLP or SDLP acted synergically with K3cit, the synergistic group induced the same amount of COD at a lower concentration or more COD formation at the same concentration, highlighting the synergistic enhancement effect of 1 + 1 > 2. At 0.3 g L−1, the COD contents induced by DLP, SDLP, K3cit, DLP–K3cit, and SDLP–K3cit synergistic groups were 20.3%, 75.8%, 75.4%, 87.3%, and 100%, respectively. The synergistic group increased the concentration of soluble Ca2+ ions in the supernatant, increased the absolute value of the zeta potential on the surface of CaOx crystals, and inhibited the aggregation among the crystals. TGA and DTG analyses established the adsorption of polysaccharides in the crystals. Cell experiments showed the ability of the synergistic group to significantly inhibit the damage of nano-COM crystals on HK-2 cells, reduce the level of reactive oxygen species and mortality, and improve cell viability and the mitochondrial membrane potential. Conclusions: The synergistic group can more effectively induce COD formation and cell protection than the standalone polysaccharide group or K3cit group. The synergistic groups, especially SDLP–K3cit, may be a potential drug for inhibiting the formation of CaOx kidney stones.
研究领域:
医学
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生物化学
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产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
·
2025
·
IF 14.2
Kaiyang Huang,Cheng Zheng,Xueyu Huang,Bangquan Wei,Lepeng Chen,Gaocan Li,Li Yang,Yunbing Wang
Glutaraldehyde cross-linked bioprosthetic heart valves (BHVs) have been widely used in clinical practice for its superior hemodynamic properties and significantly reduced requirement on anticoagulant therapy. However, the lifespan and biocompatibility of BHVs remain limited due to thrombus accumulation, poor endothelialization, inflammation, and progressive calcification. In this study, we introduced heparin-like fucoidan and recombinant humanized type III collagen (rhCol III) into glutaraldehyde cross-linked BHVs (GLUT) through photoinduced polymerization, thereby preparing an integrated hydrogel functionalized BHV (Fu-rhCol III). This multifunctional hydrogel formed a biocompatible barrier, providing robust protection for internal valve fibers and exhibiting strong resistance to platelet adhesion and thrombus formation in vitro. The Fu-rhCol III effectively reduced the cytotoxicity of GLUT, accelerating the endothelialization process. Fu-rhCol III maintained the structural stability and mechanical properties brought by glutaraldehyde cross-linking. This composite hydrogel demonstrated the significant suppression of acute inflammatory responses and satisfactory anti-calcification effect in subcutaneous implantation test. 60-day implantation results indicated that calcium deposition of Fu-rhCol III decreased by 96% compared with GLUT. In summary, this integrated hydrogel modification provides a promising strategy for future design of BHVs.
研究领域:
生物医学工程
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心血管医学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）