FOOD CHEMISTRY
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2016
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IF 4.052
Zhiqiang Xiong,Wei Liu,Lei Zhou,Liqiang Zou,Jun Chen
It has been revealed that some polyphenols can prevent enzymatic browning caused by polyphenoloxidase (PPO). Apigenin, widely distributed in many fruits and vegetables, is an important bioactive flavonoid compound. In this study, apigenin exhibited a strong inhibitory activity against PPO, and some reagents had synergistic effect with apigenin on inhibiting PPO. Apigenin inhibited PPO activity reversibly in a mixed-type manner. The fact that inactivation rate constant ( k ) of PPO increased while activation energy ( E a ) and thermodynamic parameters (Δ G , Δ H and Δ S ) decreased indicated that the thermosensitivity and stability of PPO decreased. The conformational changes of PPO were revealed by fluorescence emission spectra and circular dichroism. Atomic force microscopy observation suggested that the dimension of PPO molecules was larger after interacting with apigenin. Moreover, computational docking simulation indicated that apigenin bound to PPO and inserted into the hydrophobic cavity of PPO to interact with some amino acid residues.
研究领域:
生物化学
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食品科学
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分子生物学
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酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
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2020
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IF 1.467
Shuangyang Chen,Benard Muinde Kimatu,Donglu Fang,Xin Chen,Guitang Chen,Qiuhui Hu,Liyan Zhao
A study on the transformations of six arsenic species (arsenocholine (AsC), arsenobetaine (AsB), dimethylarsinic acid (DMA), monomethylarsonic acid (MMA), arsenite (As(III)), and arsenate (As(V))) was carried out in aqueous solution by ultrasonic treatment. The transformations of arsenic species increased with time. As the ultrasonic power or temperature was increased, transformations of arsenic species were at first enhanced and then decreased. AsC, AsB, As(III) were the most unstable at 600 W and 20 °C. DMA and MMA were stable; AsC was partially transformed to AsB, trimethylarsinic oxide (TMAO), and the more toxic tetramethylarsonium ion (TMA+); AsB was partially transformed to TMAO; and As(III) was easily oxidized to As(V). The reduction of As(V) to As(III) was only observed when the temperature was over 60 °C. The ultrasound treatment conditions with relatively stable arsenic species were 150 to 300 W, 40 to 80 °C, and 0 to 10 min. The relatively stable (150 W, 10 min, 80 °C) and unstable (600 W, 60 min, 20 °C) ultrasonic condition were applied to mushrooms and the transformations of AsC, AsB, and As(III) were observed. Currently, ultrasound-assisted extraction methods have only focused on the extraction efficiency of arsenic species and ignored their transformations. This study may serve as a guide for improving extraction efficiency without minimal transformation of the arsenic forms to reflect the actual contents in edible mushrooms. The study also provides a theoretical basis for making an accurate risk evaluation of human exposure by taking account of possible arsenic transformations during processing prior to consumption.
研究领域:
食品科学
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环境毒理学
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分析化学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
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2020
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IF 5.778
Qicai Zhang,Xianli Wang,Qinxiong Rao,Shanshan Chen,Weiguo Song
An improved understanding of imidacloprid (IMI) metabolism and accumulation in casing soil or compost-mushroom systems will help to optimise the safe use of IMI for agricultural pest control in Agaricus bisporus cultivation. In this study, the dissipation, metabolites and accumulation of IMI in casing soil or compost- A. bisporus systems were investigated. The results show that the IMI dissipation half-lives at doses of 10 and 50 mg kg −1 were 65 d and 59 d in casing soil and 6.6 d and 6.2 d in compost, respectively during the cultivation period. Three IMI metabolites were observed in casing soil during cultivation of mushrooms. Urea-imidacloprid (IMI-urea) was a major metabolite, accounting for more than 59%. In compost, the main metabolite in the first 5 days was IMI-urea, from 5 to 10 days olefin-imidacloprid (IMI-ole) and 6-chloronicotinic acid (6-CNA) were both the most prominent compounds, and after 10 days 6-CNA alone. At 50 mg kg −1 dose, IMI and the metabolites (IMI-urea, IMI-ole) were detected in the fruiting body with IMI applied to casing soil and only the metabolite 6-CNA was detected in fruiting body with IMI applied to compost. The bio concentration factors (BCFs) of IMI-ole and IMI-urea were higher than of IMI and 6-CNA, and the BCFs had no obvious relationship with log K ow . Furthermore, the potential dietary risk of IMI in A. bisporus was acceptable when application rates in casing soil or compost was up to 50 mg kg −1 . Our study supports a safe use of IMI as agricultural pest control in A. bisporus cultivation.
研究领域:
农业科学
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环境科学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2016
·
IF 2.624
Lei Zhou,Wei Liu,Zhiqiang Xiong,Liqiang Zou,Junping Liu,Junzhen Zhong,Jun Chen
Polyphenoloxidase (PPO) plays an important role in the browning of vegetables, fruits and edible fungi. The effects of ultrasound, malic acid, and their combination on the activity and conformation of mushroom ( Agaricus bisporus ) PPO were studied. The activity of PPO decreased gradually with the increasing of malic acid concentrations (5–60 mM). Neither medium concentrations (10, 20, 30 mM) malic acid nor individual ultrasound (25 kHz, 55.48 W/cm 2 ) treatment could remarkably inactivate PPO. However, the inactivation during their combination was more significant than the sum of ultrasound inactivation and malic acid inactivation. The inactivation kinetics of PPO followed a first-order kinetics under the combination of ultrasound and malic acid. The conformation of combination treated PPO was changed, which was reflected in the decrease of α-helix, increase of β-sheet contents and disruption of the tertiary structure. Results of molecular microstructure showed that ultrasound broke large molecular groups of PPO into small ones. Moreover, combined treatment disrupted the microstructure of PPO and molecules were connected together.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
高影响因子
Advanced Sustainable Systems
·
2024
·
IF 6.5
Quanxin Duan,Yushan He,Xiang Long,Jiayang Wang,Changjian Ni,Shaogui Wu
Carbon dots (CDs) have emerged as versatile fluorescent nanomaterials for diverse applications. The synthesis of CDs from natural products offers a sustainable alternative to chemical methods. This study presents the preparation of green fluorescent CDs (G-CDs) using seafood mushrooms as the carbon source and 5-amino-2-methylphenol as the nitrogen source through a hydrothermal approach. The G-CDs exhibit maximum excitation/emission peaks at 467/519 nm and a high quantum yield of up to 17.2% in ethanol. Additionally, the G-CDs demonstrate excellent photostability, salt tolerance, and chemical stability. The excitation-independent emissions suggest the presence of unique surface-state luminescent centers. The application of the G-CDs as an anti-counterfeiting fluorescent ink is demonstrated. This work highlights the promise of converting natural food sources into high-performance CDs, expanding their utility across various domains including biomedicine, sensing, and security.
研究领域:
材料科学
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生物医学
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食品安全
✓ 已验证
产品关联
DOI
高影响因子
Carbohydrate Polymer Technologies and Applications
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2021
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IF 0
Ruquan Zhang,Qianru Xu,Yongzhen Tao,Xinyu Wang
Hydrogels with thixotropic and pH-sensitive properties exhibit injectablity and response to external stimulation. Thixotropic and pH-responsive hydrogels were fabricated from xanthan gum (XG) and a hyperbranched β -D-glucan (TM3a) crosslinked with sodium trimetaphosphate (STMP) for injectable and release-controlled application. Thixotropy and creep-recovery tests were carried out to determine injectable and viscoelastic properties of the XG-TM3a-STMP hydrogels. Introduction of TM3a and formation of chemically crosslinking structures resulted in higher yield stress (43.6–168.9 Pa) and elastic modulus (146.9–1245.7 Pa), lower retardation time (32.6–44.4 s), and higher recovery percentage (49.5–66.7%) for XG-TM3a-STMP compared to XG-STMP and XG-TM3a-water. FTIR spectra and SEM observation demonstrated that TM3a disassembled XG aggregates by partially breaking hydrogen bonds, resulting in a relatively thin wall of the grids for XG-TM3a-STMP. The XG-TM3a-STMP hydrogels exhibited pH dependant swelling property and controlled release behavior. The hybrid hydrogels have potential applications in 3D printing or injectable tissue engineering and drug release fields.
研究领域:
生物材料学
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药物递送系统
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组织工程
✓ 已验证
产品关联
DOI
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2017
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IF 3.582
Bin-Bin Hu,Ming-Jun Zhu
Clostridium thermocellum 27405 is a potential consolidated bioprocessing (CBP) strain due to the efficient lignocellulose degradation ability. Here, the spent mushroom substrate (SMS), a lignocellulosic byproduct of agriculture, was used as a substrate for hydrogen production by C. thermocellum . SMS showed a good performance for hydrogen production and the hydrogen production was significantly enhanced by C. thermocellum supplemented with β-glucosidases, with the highest production reaching 56.0 mM, an increase of 37%. Additionally, β-glucosidase and Triton X-100 had a synergistic effect on biological saccharification , resulted in an accumulation of 5.06 g/L reducing sugars, an increase of 28%. However, fusion β-glucosidase showed no improvement on hydrogen production and biological saccharification. The present study demonstrates the different influences of β-glucosidase and fusion β-glucosidase on hydrogen production and biological saccharification in CBP system and provides valuable information on the biodegradation of SMS using C. thermocellum .
研究领域:
微生物学
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生物工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Jiaxu Yao,Li Zhou,Yueqi Hu,Minjie Zhao,Yue Ma,Jikai Liu,Eric Marchioni
The global phenomenon of eating poisonous mushrooms by mistake occurs every year. Untargeted lipidomics analysis combined with chemometrics was used to identify mushroom varieties. Two kinds of mushrooms with similar appearance, namely, Pleurotus cornucopiae (P. cornucopiae) and Omphalotus japonicus (O. japonicus) were selected as models, where O. japonicus was a poisonous mushroom and P. cornucopiae was an edible mushroom. First, the lipid extraction efficiency of eight solvents was compared. The methyl tert-butyl ether/methanol (2:1, v/v) had higher lipid extraction efficiency of extracting mushroom lipids than other solvents, in terms of the lipid coverage, response intensity, and solvent safety. Afterward, the comprehensive lipidomics analysis of the two mushrooms was conducted. A total of 21 lipid classes and 267 molecular species were identified in O. japonicus, whereas 22 lipid classes and 266 molecular species in P. cornucopiae. The principal component analysis demonstrated that 37 characteristic metabolites, including TAG 18:1_18:2_18:0;1O, TAG 18:1_18:1_18:2, TAG 16:2_18:2_18:2, etc., could be used to distinguish the two mushrooms. These differential lipids were able to identify P. cornucopiae blended with 5% (w/w) O. japonicus. This study explored a novel method for identifying poisonous mushrooms from edible mushrooms and provided a reference for food safety of consumers.
研究领域:
食品安全
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分析化学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Heliyon
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2020
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IF 0
Tao Feng,Zhongshan Hu,Yanzun Tong,Lingyun Yao,Haining Zhuang,Xiao Zhu,Shiqing Song,Jun Lu
Chicken liver is a main protein source to prepare attractant for dog food. However, animal proteins are costly. Seeking high quality and low-cost protein sources has been a goal for the industry. Mushroom Lentinus edodes (L. edodes) and Mealworm Tenebrio molitor (T. molitor) are novel protein sources, showing high potential as raw material of attractants. In this paper, chicken liver, L. edodes, and T. molitor were used as three different protein sources to prepare attractants. Their palatability to dogs were then compared. Firstly, the enzymatic hydrolysis process of three proteins was optimized, with a degree of hydrolysis of 54.82%, 36.10% and 30.14% for chicken liver, L. edodes , and T. molitor respectively. Secondly, volatile compounds of three attractants were identified by HS-SPME/GC-MS and SDE/GC-MS. Using OAV and PLRS method, it was found that bis(2-methyl-3-furyl) disulfide, indole, methional, 2-(methyl thio) phenol, γ -butyrolacton, furfuryl alcohol, acetic acid and isovaleraldehyde were the key components. Although both T. molitor and L. edodes attractant showed less palatability than that of chicken liver, they could be readily improved via adding key palatable volatile compounds. The ingestion rate of dog food with attractant showed a similar trend and was higher than that of food without attractant.
研究领域:
食品科学
·
动物营养学
✓ 已验证
DOI
PubMed
ACS Omega
·
2021
·
IF 3.512
Daihua Hu,Xu Yang,Chingyuan Hu,Zili Feng,Wang Chen,Hanmeng Shi
Vitamin D deficiency is a severe worldwide health issue. Edible mushrooms are an excellent vitamin D2 source and have gained popularity worldwide as a nutritional food. The objective of this study was to investigate the conversion efficiency of ergosterol to vitamin D2 in Agaricus bisporus and Cordyceps militaris mushrooms under ultraviolet (UV) irradiation directly through dry powder or in ethanol suspension (1:20 g/mL, solid to liquid ratio). Several parameters of UV irradiation conditions such as the material form (dry powder or dry powder in ethanol suspension), exposure time (30, 60, or 120 min), wavelength type (UV-C, UV-B, or UV-A), wavelength combination (UV-C plus UV-B, UV-C plus UV-A, UV-B plus UV-A, or UV-C plus UV-B plus UV-A), and wavelength sequence (UV-C → UV-B, UV-C → UV-A, UV-B → UV-A, or UV-C → UV-B → UV-A), were optimized. Under the optimal UV irradiation conditions (dry powder in ethanol suspension irradiated with UV-C at 40 cm for 120 min), vitamin D2 concentrations increased from not detectable to 72 μg/g (dw) in the A. bisporus dry powder and 1104 μg/g (dw) (about 15-fold increase) in the ethanol suspension. After UV irradiation, the vitamin D2 concentration increased from undetectable to 57 μg/g (dw) in the C. militaris dry powder. In contrast, UV irradiation increased the concentration to 877 μg/g (dw) (about 15-fold higher) in the ethanol suspension. Comparison of the effect of various wavelength combinations showed that UV-C irradiation is more effective than UV-A or UV-B. Furthermore, when irradiated by UV-C at a 40 cm irradiation distance in the ethanol suspension, the increase in vitamin D2 in A. bisporus and C. militaris mushrooms was time- or dose-dependent. The conversion rate of vitamin D2 was low to undetectable under dry powder irradiation, but its ergosterol loss rate was higher than in ethanol suspension irradiation. The ergosterol loss rate in dry C. militaris mushrooms was higher than in the dry A. bisporus mushroom powder. Ultraviolet irradiation in ethanol suspension could greatly increase the vitamin D2 concentration than directly on the dry powder and thus make edible mushrooms more practical as a natural vitamin D source for consumers after entirely removing the ethanol.
研究领域:
营养学
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食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2018
·
IF 5.116
Yaoyao Ning,Dandan Ma,Yu Shen,Fumin Wang,Xubin Zhang
Electrochemical water splitting into hydrogen energy offers a promising pathway for renewable and clean energy conversion and storage, but high overpotentials are needed to drive the process owing to the sluggish kinetics of the anodic oxygen evolution reaction (OER) and the cathodic hydrogen evolution reaction (HER). Herein, three-dimensional (3D) mushroom-like NiCo/NiCo 2 S 4 @NiCo arrays grown directly on Ni foam (NF) have been constructed via hydrothermal and electrodeposition methods. Owing to the unique mushroom-like structure and the beneficial effect of the composite catalyst, the NiCo/NiCo 2 S 4 @NiCo/NF electrode exhibits excellent activity and stability for both OER and HER. When serving as an anode electrocatalyst , the composite catalyst only requires a overpotential of 294 mV to achieve a high current density of 100 mA/cm 2 for the OER in 1 M KOH. Moreover, in chronopotentiometric and chronoamperometric measurements, the NiCo/NiCo 2 S 4 @NiCo/NF electrode displays distinguished electrochemical and structural stability for at least 100,000 s. Similarly, the NiCo/NiCo 2 S 4 @NiCo/NF catalyst delivers a hydrogen production current density of 10 mA/cm 2 at an overpotential of 132 mV and shows no obvious attenuation of performance after stability testing for 100,000 s in an extreme alkaline environment. Accordingly, the remarkable and stable bifunctional abilities enable the application of the NiCo/NiCo 2 S 4 @NiCo/NF catalyst in two-electrode water-alkali electrolyzers with a low cell voltage of 1.55 V to deliver a current density of 10 mA/cm 2 and excellent stability for over 27 h.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Zhilun Liu,Lei Zhao,Shuang Lu,Xiaohan Hou,Ding Hou,Bo Sun,Min Zhao,Xueyan Li,Jun Ma
In recent years, biochar catalysts have shown great potential in activating persulfate (PS) for organic pollutant degradation in water. However, current challenges persist in biochar-activated PS systems: variations in pyrolysis conditions and precursors compromise long-term operational stability, complex aqueous environments lead to suboptimal degradation efficiency, and activation mechanisms remain scientifically contentious. The characterizations of the mushroom derived biochar (MBC) showed the unique sieve tube structure, rich surface functional groups and large specific surface area (SSA) (632.08 m 2 /g). The rich surface functional groups and large SSA could facilitate mass transfer and provide rich active sites for activating peroxymonosulfate (PMS). In the MBC-800/PMS system, the degradation efficiency of ketoprofen (KTP) could reach 88.83 %, which higher than other biomass derived biochar and many synthetic activators. The free radical degradation and non-free radical degradation were both involved in this activation reaction, and the sulfate radicals (SO 4 •– ) were the dominant oxidative species for KTP degradation. According to the XPS analysis results, the ketone groups and carboxyl groups were the active sites for activating PMS. In addition, the main intermediates of KTP were analyzed and the pathway of KTP degradation was investigated. This study could provide an effective catalyst for activating PMS and insight into the mechanism of the radicals produced in the biochar/PMS system, which could provide high value for the research of biomass activator to activate PMS.
研究领域:
环境科学
·
催化材料
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）