Biomolecules
·
2023
·Vol. 13
·期 3
·496
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IF 5.5
Yingyuan Zhao,Zhaoxuan Wang,Shuxian Liu,Shiying Xie,Yinchun Xie,Ruifang Li,Hiroaki Oda
Astaxanthin is a carotenoid with excellent antioxidant activity. However, this small lipid-soluble molecule is insoluble in water and has low stability. Although this situation can be improved when astaxanthin is prepared as a nanosuspension, the aqueous form is still not as convenient and safe as the dry powder form for storage, transport, and use. The lyophilization process provides better protection for thermosensitive materials, but this leads to collapse and agglomeration between nanoparticles. To improve this situation, appropriate lyophilization protectants are needed to offer support between the nanoparticles, such as sugars, amino acids, and hydroxy alcohols. The purpose of this work is to screen lyophilization protectants by single-factor experiments and response surface optimization experiments and then explore the optimal ratio of compound lyophilization protectants, and finally, make excellent astaxanthin/BSA/chitosan nanosuspension (ABC-NPs) lyophilized powder. The work shows that the optimal ratio of the compounding lyophilization protectant is 0.46% oligomeric mannose, 0.44% maltose, and 0.05% sorbitol (w/v). The ABC-NPs lyophilized powder prepared under the above conditions had a re-soluble particle size of 472 nm, with a ratio of 1.32 to the particle size of the sample before lyophilization. The lyophilized powder was all in the form of a pink layer. The sample was fluffy and dissolved entirely within 10 s by shaking with water. Consequently, it is expected to solve the problem of inconvenient storage and transportation of aqueous drugs and to expand the application of nanomedicine powders and tablets.Keywords:lyophilization;astaxanthin nanoparticles;freeze-drying protectant
研究领域:
药学
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纳米技术
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生物技术
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 17
·4201
·
IF 4.2
Wenxuan Jiang,Xiangrong Deng,Lanxian Qin,Dahai Jiang,Mengqi Lu,Kai Chen,Manqi Yang,Liangliang Zhang,Jianchun Jiang,Liming Lu
This study focused on developing an effective cell wall-breaking method forPhaffia rhodozyma, followed by utilizing subcritical fluid extraction to isolate, extract, and concentrate astaxanthin from the complex fermentation products ofP. rhodozyma. A comprehensive comparison of seven distinct methods for disrupting cell walls, including dimethyl sulfoxide treatment, lactic acid treatment, sodium hydroxide treatment,β-glucanase enzymatic digestion,β-mannanase enzymatic digestion, and a combined enzymatic treatment involving bothβ-mannanase andβ-glucanase was conducted. The results identified the lactic acid method as the most effective in disrupting the cell walls ofP. rhodozyma. The software, Design Expert, was used in the process of extracting astaxanthin from cell lysates using a subcritical extraction method. Through fitting analysis and response surface optimization analysis by Design Expert, the optimal extraction conditions were determined as follows: an extraction temperature of 41 °C, extraction frequency of two times, and extraction time of 46 min. These parameters facilitated the efficient extraction, concentration, and enrichment of astaxanthin fromP. rhodozyma, resulting in an astaxanthin concentration of 540.00 mg/L. This result can establish the foundation for its high-value applications.
研究领域:
生物技术
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食品科学
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DOI
PubMed
高影响因子
Marine Life Science & Technology
·
2025
·
IF 5.3
Liu Kangjing,Tian Xueying,Fei Siyuan,Song Yukun,Abd El-Aty A. M.,Tan Mingqian
This study demonstrated the design of whey protein isolate (WPI)-mannose (Man) conjugates with triphenylphosphonium bromide (TPP) through self-assembly to prepare macrophage and mitochondrion dual-targeting astaxanthin (AXT) nanoparticles (AXT@TPP-WPI-Man). The nanoparticles displayed spherical structures with a well-dispersed size of approximately 206.1 ± 39.2 nm, with good biocompatibility, stability, and targeting capabilities. In vitro experiments demonstrated the specific accumulation of AXT@TPP-WPI-Man in mitochondria and exhibited good targeting ability toward macrophages. The AXT@TPP-WPI-Man effectively reduced reactive oxygen species and preserved the normal mitochondrial membrane potential. The AXT@TPP-WPI-Man treated ulcerative colitis mice exhibited a 52.32% increase in colon length with significant improvement in weight loss, disease activity index scores, and reduced release of inflammatory cytokines. Immunofluorescence staining indicated AXT@TPP-WPI-Man alleviated ulcerative colitis by reducing M1 polarization in colonic macrophages while promoting M2 polarization. The dual-targeting AXT@TPP-WPI-Man has the potential to improve astaxanthin bioavailability, presenting a promising delivery method for the treatment of ulcerative colitis.
研究领域:
生物医学工程
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药理学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xiaoting Yu,Xunyu Song,Yannan Chen,Yueqi Lv,Mingqian Tan
Astaxanthin (AXT) with mitochondria-targeted ability is desired for effectively preventing oxidative stress in various intestinal diseases. However, it is challenging to prepare mitochondria-targeted AXT (Mito-AXT) using traditional covalent bond methods due to the fragile structure of AXT and harsh reaction conditions, which result in low production yields and poor biocompatibility. Herein, we proposed a strategy to enhance the mitochondria-targeted efficiency of AXT by combining it with lipophilic cationic compounds through a non-covalent approach. Mito-AXT was synthesized through electrostatic coupling between anionic sulfonate-modified AXT (AXT-SO 3 ) and cationic (3-carboxypropyl) triphenylphosphonium bromide (TPP). The synthetic strategy successfully preserved the inherent DPPH and hydroxyl radical trapping capacity of AXT and achieved the efficient preparation of Mito-AXT with a yield of 86.17%. The accumulation of cationic TPP in mitochondria after the disassembly of Mito-AXT surprisingly facilitated the enrichment of anionic AXT-SO 3 in mitochondria. Notably, Mito-AXT regulated the redox balance, mitochondrial membrane potential, and mitophagy in IEC-6 cells under oxidative stress, thereby preventing apoptosis, compared to TPP-modified AXT molecules formed by covalent bonds. Furthermore, biosecurity analysis confirmed that Mito-AXT exhibited excellent biocompatibility. The results provided a simple, effective, and safe way to enhance the mitochondria-targeted efficiency of AXT through Mito-AXT, thereby effectively regulating the mitochondrial homeostasis in intestinal cells under oxidative stress.
研究领域:
生物化学
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药理学
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细胞生物学
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Shujie Wang,Yuyue Qin,Yaping Liu,Guoqin Liu,Guiguang Cheng,Thanapop Soteyome
In this study, three types of β -sitosterol-based oleogels ( β -sitosterol + γ -oryzanol oleogels, β -sitosterol + lecithin , oleogels and β -sitosterol + monostearate oleogels), loaded with astaxanthin, were employed as the oil phase to create oleogel-based emulsions (SO, SL, and SM) using high-pressure homogenization. The microstructure revealed that fine-scale crystals were dispersed within the oil phase of the droplets in the β -sitosterol oleogel-based emulsion. The bioaccessibility of astaxanthin was found to be 58.13 %, 51.24 %, 36.57 %, and 45.72 % for SM, SL, SO, and the control group, respectively. Interestingly, the release of fatty acids was positively correlated with the availability of astaxanthin (P = 0.981). Further analysis of FFAs release and kinetics indicated that the structural strength of the oil-phase in the emulsions influenced the degree and rate of lipolysis. Additionally, the micellar fraction analysis suggested that the nature and composition of the oleogelators in SM and SL also impacted lipolysis and the bioaccessibility of astaxanthin. Furthermore, interfacial binding of lipase and isothermal titration calorimetry (ITC) measurements revealed that the oleogel network within the oil phase of the emulsion acted as a physical barrier, hindering the interaction between lipase and lipid. Overall, β-sitosterol oleogel-based emulsions offer a versatile platform for delivering hydrophobic molecules, enhancing the bioavailability of active compounds, and achieving sustained release.
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
·
2024
·
IF 2.5
Shujie Cheng,Shimin Wu,Jianqiang Lan
Polycyclic aromatic hydrocarbons (PAHs) generated from heating butter pose a potential threat to consumer health. This study involved heating butter under different conditions and analysing the changes in the content of 54 PAHs. Furthermore, we investigated the inhibitory effects of β-carotene, pure astaxanthin and astaxanthin microcapsule powder on PAH formation. Results showed that heating temperature and duration affect the formation and transformation of PAHs, with higher temperatures facilitating the synthesis of oxygenated PAHs, whereas longer heating times promote the accumulation of light PAHs. Despite an observed increase in the concentration of light PAHs following heat treatment with added antioxidants, the antioxidants significantly reduced the toxic equivalency quotients (TEQs) in butter. This study revealed the PAH production pattern during butter heating and the effectiveness of antioxidants in inhibiting their accumulation.
研究领域:
食品科学
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毒理学
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营养学
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产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Jia Jianping,Chen Zhitao,Li Qingqing,Li Feifei,Liu Siru,Bao Guoliang
Astaxanthin has multiple physiological functions and is applied widely. The yeast Phaffia rhodozyma is an ideal source of microbial astaxanthin. However, the stress conditions beneficial for astaxanthin synthesis often inhibit cell growth, leading to low productivity of astaxanthin in this yeast. In this study, 1 mg/L melatonin (MT) could increase the biomass, astaxanthin content, and yield in P. rhodozyma by 21.9, 93.9, and 139.1%, reaching 6.9 g/L, 0.3 mg/g DCW, and 2.2 mg/L, respectively. An RNA-seq-based transcriptomic analysis showed that MT could disturb the transcriptomic profile of P. rhodozyma cell. Furthermore, differentially expressed gene (DEG) analysis show that the genes induced or inhibited significantly by MT were mainly involved in astaxanthin synthesis, metabolite metabolism, substrate transportation, anti-stress, signal transduction, and transcription factor. A mechanism of MT regulating astaxanthin synthesis was proposed in this study. The mechanism is that MT entering the cell interacts with components of various signaling pathways or directly regulates their transcription levels. The altered signals are then transmitted to the transcription factors, which can regulate the expressions of a series of downstream genes as the DEGs. A zinc finger transcription factor gene (ZFTF), one of the most upregulated DEGs, induced by MT was selected to be overexpressed in P. rhodozyma. It was found that the biomass and astaxanthin synthesis of the transformant were further increased compared with those in MT-treatment condition. Combining MT-treatment and ZFTF overexpression in P. rhodozyma, the biomass, astaxanthin content, and yield were 8.6 g/L, 0.6 mg/g DCW, and 4.8 mg/L and increased by 52.1, 233.3, and 399.7% than those in the WT strain under MT-free condition. In this study, the synthesis and regulation theory of astaxanthin is deepened, and an efficient dual strategy for industrial production of microbial astaxanthin is proposed.
研究领域:
微生物学
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生物化学
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基因工程
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产品关联
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2023
·
IF 5.1
Swagata Dutta,S.P. Jeevan Kumar,Rintu Banerjee
Astaxanthin , a pink/red pigmented keto carotenoid occurring in certain algae, is an anti-oxidant that protects the cell from damage by attenuating the reactive oxygen species due to which it has immense potential to protect the skin and other allied cells. Commercially available chemical UV-filters used in sunscreen creams and lotions are not only inadequate in protecting the skin against the harmful UV-rays but also cause skin damage like skin irritation , skin-aging, allergic reactions upon prolong exposure to these chemicals. Moreover, they cause environmental pollution and damage, mainly to aquatic life . So, natural compounds having similar or improved protective potential against UV irradiated skin are in dire need in the cosmetics industry. Recent studies substantiate that astaxanthin can be employed in sunscreens owing to its high antioxidant potential and significant use as an anti-aging, anti-inflammatory, and anti-cancer compound. Astaxanthin accumulation has been found in microalgae and its properties suggest that astaxanthin can provide total protection against all the UVR related skin problems starting from the acute effects of sunburn, pigmentation , erythema to chronic effects of inflammation, photoaging and skin cancer. In this paper, astaxanthin's importance, sources, chemical structure, pilot scale production and its potential applications in cosmetic industry have been discussed.
研究领域:
生物化学
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化妆品科学
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环境科学
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Yu-Hong Liu,Adili Alimujiang,Xiang Wang,Shan-Wei Luo,Srinivasan Balamurugan,Wei-Dong Yang,Jie-Sheng Liu,Lin Zhang,Hong-Ye Li
Haematococcus pluvialis is a main biological resource for the antioxidant astaxanthin production, however, potential modulators and molecular mechanisms underpinning astaxanthin accumulation remain largely obscured. We discovered that provision of ethanol (0.4%) significantly triggered the cellular astaxanthin content up to 3.85% on the 4th day of treatment. Amongst, 95% of the accumulated astaxanthin was esterified, particularly enriched with monoesters. Ultrastructural analysis revealed that ethanol altered cell wall structure and physiological properties. Antioxidant analyses revealed that astaxanthin accumulation offset the ethanol induced oxidative stress. Ethanol treatment reduced carbohydrates while increased lipids and jasmonic acid production. Transcriptomic analysis uncovered that ethanol orchestrated the expression of crucial genes involved in carotenogenesis, e.g. PSY, BKT and CRTR-b were significantly upregulated. Moreover, methyl jasmonic acid synthesis was induced and played a major role in regulating the carotenogenic genes. The findings uncovered the novel viewpoint in the intricate transcriptional regulatory mechanisms of astaxanthin biosynthesis.
研究领域:
植物学
·
分子生物学
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生物化学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Zhe Lu,Jingcheng Dai,LingLing Zheng,Zhuoran Teng,Qi Zhang,Dongru Qiu,Lirong Song
Improving carbon availability in astaxanthin production is pivotal in Haematococcus industry. In this study, disodium 2-oxoglutarate (2-OG-2Na) was observed to be a potential carbon regulator to increase the astaxanthin content. To illustrate its efficacy in astaxanthin production, key genes and enzyme were analyzed. Upon 2-OG-2Na treatment, genes ipi , bkt and crtR-b were up regulated, concomitantly, carotenoids and astaxanthin content increased by 15.4% and 14.0% at 120 h, respectively; additionally, Acetyl-CoA carboxylase was activated, consistent with 1.27-fold increase in fatty acids content. PUFAs increased earlier as fatty acids assembly gene fad was up-regulated to 20.56. It was also found that cell division was not compromised. Altogether, it was suggested that increased carbon skeletons were re-directed into the astaxanthin and fatty acids biosynthesis pathway. Furthermore, 2-OG-2Na was applied in ten Haematococcus strains. Of these strains, astaxanthin contents were accelerated with average net increase of 10.48%, exhibiting a scalable paradigm for commercial production.
研究领域:
生物化学
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植物学
·
工业微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Xiang Wang,Jin-Hua Mou,Zi-Hao Qin,Ting-Bin Hao,Lan Zheng,Joseph Buhagiar,Yu-Hong Liu,Srinivasan Balamurugan,Yuhe He,Carol Sze Ki Lin,Wei-Dong Yang,Hong-Ye Li
The unicellular freshwater green alga Haematococcus pluvialis has attracted much research attention due to its biosynthetic ability for large amounts of astaxanthin, a blood-red ketocarotenoid that is used in cosmetics, nutraceuticals, and pharmaceuticals. Recently, numerous studies have investigated the functions of natural astaxanthin; however, the high cost of the production of astaxanthin from H. pluvialis cultures restricts its commercial viability. There is an urgent need to fulfill commercial demands by increasing astaxanthin accumulation from H. pluvialis cultures. In this study, we discovered that treatment of H. pluvialis cultures at the beginning of the macrozooid stage (day 0) with 1 μM rac-GR24, a synthetic analogue of strigolactones (a class of phytohormones), led to significant increases in biomass [up to a maximum dry cell weight (DCW) of 0.53 g/L] during the macrozooid stage and astaxanthin (from 0.63 to 5.32% of DCW) during the hematocyst stage. We elucidated that this enhancement of biomass accumulation during the macrozooid stage by rac-GR24 is due to its increasing CO2 utilization efficiency in photosynthesis and carbohydrate biosynthesis. We also found that rac-GR24 stimulated the overproduction of nicotinamide adenine dinucleotide phosphate (NADPH) and antioxidant enzymes in H. pluvialis cultures, which alleviated the oxidative damage caused by reactive oxygen species generated during the hematocyst stage due to the exhaustion of nitrogen supplies. Moreover, rac-GR24 treatment of H. pluvialis synergistically altered the activity of the pathways of fatty acid biosynthesis and astaxanthin esterification, which resulted in larger amounts of astaxanthin being generated by rac-GR24-treated cultures than by controls. In summary, we have developed a feasible and economic rac-GR24-assisted strategy that increases the amounts of biomass and astaxanthin generated by H. pluvialis cultures, and have provided novel insights into the mechanistic roles of rac-GR24 to achieve these effects.
研究领域:
植物学
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植物化学
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生物技术
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药理学
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Shi Zhihui,He Xiaoxian,Zhang Hailiang,Guo Xuena,Cheng Yanfei,Liu Xuelian,Wang Zhaoyue,He Xiuping
Carotenoids are unsaturated compounds with terpene groups. Among them, astaxanthin has strong antioxidant properties. It is widely used in aquaculture, food, medicine, and cosmetics with a broad market prospect. Phaffia rhodozyma is an important microorganism that synthesizes astaxanthin, but its wild strains have low pigment content, long growth cycle, and low fermentation temperature. Therefore, it is important to research the genetic improvement of the physiological and biochemical properties of P. rhodozyma. In this study, the atmospheric and room temperature plasma mutagenesis technology was adopted, through the functional evolution of the carotenoid production performance; then, through the comparative analysis of the genomics and transcriptomics of the wild strain and evolved strain, the key factor GST1 gene that affects carotenoid synthesis was discovered.
研究领域:
微生物学
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遗传学
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生物化学
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产品关联
DOI
PubMed
高影响因子
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