RSC Advances
·
2015
·Vol. 5
·期 93
·76526-76537
·
IF 3.84
Jiabing Ran,Jingxiao Hu,Guanglin Sun,Si Chen,Li Chen,Xinyu Shen,Hua Tong
In this research, gelatin-tussah silk fibroin/hydroxyapatite (GEL-TSF/HAp), gelatin-Bombyx mori silk fibroin/hydroxyapatite (GEL-BMSF/HAp), and gelatin/hydroxyapatite (GEL/HAp) nano-composites were synthesized by a novel in situ precipitation method. Characterizations, including surface morphology, elemental composition and distribution, structure of the crystalline phase, mechanical strength, thermal stability, and in vitro cytocompatibility, were carried out. Investigations on the crystalline phase showed that rod-like HAp crystallites in the GEL-TSF/HAp composite had a higher aspect ratio than those in the GEL-BMSF/HAp composite and the GEL/HAp composite. In addition, the GEL-TSF/HAp composite also presented better thermal stability than the other two composites revealed by differential thermal analysis (DTA) and thermogravimetric analysis (TGA). Mechanical properties testing indicated that the GEL-TSF/HAp composite had a higher elastic modulus at low strain and higher compressive modulus at high strain simultaneously than the other two composites. An in vitro cell culture showed that MG63 osteoblast-like cells on the GEL-TSF/HAp membrane took on higher proliferative potential than those on the GEL-BMSF/HAp membrane. These results indicated that compared to the GEL-BMSF/HAp composite, the GEL-TSF/HAp composite was more suitable for bone tissue engineering (BTE) applications.
研究领域:
生物材料学
·
医学工程
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DOI
高影响因子
Toxins
·
2022
·Vol. 14
·期 7
·421
·
IF 5.075
Vivian Andoh,Liang Chen,Feifei Zhu,Qi Ge,Lin Ma,Qiang Wang,Keping Chen
Melanin has been reported to have potential applications in industries such as cosmetics and food due to its anti-UV and antioxidative qualities. However, the corresponding data on its safety evaluation or biological consequences are fairly limited; such data are critical given its widespread use. The effect of different concentrations (1, 2, 3, and 4%) of melanin on growth status (larvae length and weight, cocoon weight, and morphology), the microstructure of the various tissues (fat body, silk gland, and midgut), and silk properties was studied by using the silkworm (bombyx mori) as the model organism. The weight and length of silkworm larvae fed with melanin were lower than the control, indicating that melanin appears to have a negative effect on the growth status of silkworms; however, the histophysiology analysis indicates that the cell morphologies are not changed, the XRD and FTIR spectra indicate that the secondary and crystalline structures of silks are also well preserved, and the thermogravimetric analysis and tensile test indicate that the thermal stability and mechanical properties are well maintained and even improved to some extent. Generally, it indicates that melanin has a certain inhibitory effect on the growth of silkworm larva but causes no harm to the cell microstructures or silk properties; this demonstrates that the safety of melanin as a food addictive should be considered seriously. The increase of thermal stability and mechanical properties shows that melanin may be a good chemical modifier in textile industries.Keywords:melanin;biological effects;silkworm;silk propertiesKey Contribution:Melanin caused silkworms to lose weight. While silkworms provide an excellent platform for studying melanin toxicity, melanin substantially improved the mechanical characteristics of silkworm silk.
研究领域:
昆虫学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Natural Fibers
·
2022
·
IF 3.507
Yun Wang,Xin Liu,Liangjun Xia,Chunhua Zhang,Weilin Xu
Silk fibers were produced by feeding Bombyx mori silkworm larvae with a modified feed of mulberry leaves containing nano Fe3O4 particles. The silkworms were weighed to determine the effect of nano Fe3O4 particles on the growth of larvae. The effects of nano Fe3O4 contents on the morphologies, chemical structures, mechanical properties, and thermal properties of silk were also investigated, respectively. The results indicate that the nano Fe3O4 particles was embedded in the silk. Additionally, the stress and toughness of modified silk were enhanced, as compared with the normal silk. Finally, the integration of the amide I band intensities suggests a relatively high content of β-sheet in the modified silk.
研究领域:
生物学
·
材料科学
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产品关联
DOI
高影响因子
Materials
·
2018
·Vol. 12
·期 1
·14
·
IF 2.467
Lan Cheng,Huiming Huang,Jingyou Zeng,Zulan Liu,Xiaoling Tong,Zhi Li,Hongping Zhao,Fangyin Dai
In this study, eight types of materials including nanoparticles (Cu and CaCO3), metallic ions (Ca2+and Cu2+), and amino acid substances (serine, tyrosine, sericin amino acid, and fibroin amino acid) were used as additives in silkworm diets to obtainin-situmodified silk fiber composites. The results indicate that tyrosine and fibroin amino acids significantly increase potassium content in silk fibers and induce the transformation of α-helices and random coils toβ-sheet structures, resulting in higher crystallinities and better mechanical properties. However, the other additives-modified silk fibers show a decrease inβ-sheet contents and a slight increase or even decrease in tensile strengths. This finding provides a green and effective approach to produce mechanically enhanced silk fibers with high crystallinity on a large scale. Moreover, the modification mechanisms of these additives were discussed in this study, which could offer new insights into the design and regulation of modified fibers or composites with desirable properties and functions.Keywords:silkworm silk;additives;secondary structure;thermal and mechanical properties
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLORATION TECHNOLOGY
·
2024
·
IF 2
Na-won Baek,Shin-hee Lee
Enzymatic dyeing is an environmentally friendly and energy-efficient dyeing method conducted at low temperatures, making it an important option for sustainable dyeing processing technology. This study investigated the enzymatic dyeing of cotton and silk fabrics using six phenolic monomers (catechol, hydroquinone, 2-aminophenol, 4-aminophenol, 1,4-phenylenediamine and 2,5-diaminobenzenesulphonic acid) via oxidative polymerisation catalysed by horseradish peroxidase (HRP). The research explored the colouring mechanism, functional attributes and dyeability of the fabrics. The polymerisation mechanism of the polymer was elucidated through liquid chromatography–tandem mass spectrometry, while the functional and morphological characteristics of the dyed fabric were examined via Fourier transform–infrared spectroscopy, thermogravimetric analysis and scanning electron microscopy imaging. The dyed fabric underwent dyeability assessments, including colour depth analysis and various fastness tests. Under mild reaction conditions, nearly all the phenolic monomers successfully dyed both the silk and cotton fabrics using HRP. The dyed fabrics demonstrated enhanced thermal stability and dyeability. However, the cotton fabrics exhibited worse dyeability and fastness than the silk fabrics, with noticeable stains on their surfaces when dyed with certain phenols. These findings underscore the suitability of enzymatic oxidative dyeing for protein fibres over vegetable fibres, as well as the importance of tailoring enzymatic dyeing processes for vegetable fibres.
研究领域:
纺织科学
·
生物化学
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产品关联
DOI
高影响因子
Materials
·
2017
·Vol. 10
·期 6
·667
·
IF 2.654
Gang Tao,Rui Cai,Yejing Wang,Kai Song,Pengchao Guo,Ping Zhao,Hua Zuo,Huawei He
Bionanocomposite packaging materials have a bright future for a broad range of applications in the food and biomedical industries. Antimicrobial packaging is one of the bionanocomposite packaging materials. Silver nanoparticle (AgNP) is one of the most attractive antimicrobial agents for its broad spectrum of antimicrobial activity against microorganisms. However, the traditional method of preparing AgNPs-functionalized packaging material is cumbersome and not environmentally friendly. To develop an efficient and convenient biosynthesis method to prepare AgNPs-modified bionanocomposite material for packaging applications, we synthesized AgNPs in situ in a silk fibroin solution via the reduction of Ag+by the tyrosine residue of fibroin, and then prepared AgNPs–silk/poly(vinyl alcohol) (PVA) composite film by blending with PVA. AgNPs were synthesized evenly on the surface or embedded in the interior of silk/PVA film. The prepared AgNPs–silk/PVA film exhibited excellent mechanical performance and stability, as well as good antibacterial activity against both Gram-negative and Gram-positive bacteria. AgNPs–silk/PVA film offers more choices to be potentially applied in the active packaging field.Keywords:biosynthesis;AgNPs;silk;PVA;packaging application
研究领域:
材料科学
·
生物学
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 7
·1177
·
IF 5.719
Zhong-Feng Gao,Lin-Lin Zheng,Wen-Long Fu,Lei Zhang,Jin-Ze Li,Pu Chen
In this study, we fed the larval of Bombyx mori silkworms with nanodroplets of liquid metal (LM) coated with microgels of marine polysaccharides to obtain stretchable silk. Alginate-coated liquid metal nanodroplets (LM@NaAlg) were prepared with significant chemical stability and biocompatibility. This study demonstrates how the fed LM@NaAlg acts on the as-spun silk fiber. We also conducted a series of characterizations and steered molecular dynamics simulations, which showed that the LM@NaAlg additions impede the conformation transition of silk fibroins from the random coil and α-helix to the β-sheet by the formation of hydrogen bonds between LM@NaAlg and the silk fibroins, thus enhancing the elongation at the breakpoints in addition to the tensile properties. The intrinsically highly stretchable silk showed outstanding mechanical properties compared with regular silk due to its 814 MPa breaking strength and a breaking elongation of up to 70%—the highest reported performance so far. We expect that the proposed method can expand the fabrication of multi-functional silks.Keywords:silkworm silk;liquid metal;sodium alginate;silk fibroin;functional materials
研究领域:
生物材料学
·
纺织科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 47
·28408-28414
·
IF 3.119
Heming Zheng,Bo Duan,Zheyu Xie,Jie Wang,Mingying Yang
Bombyx mori (B. mori) silk fibroin (SF) microcapsules have acted as a great candidate in delivering drugs. However, it is difficult to fabricate SF nanocapsules using the present layer-by-layer (LBL) technique. In addition, the current SF microcapsules have limits in loading negatively charged drugs. Here, we invent a novel LBL method by introducing silane (APTES) as a structure indicator to produce SF nanocapsules that can load drugs with negative or positive charge. LBL assembly was completed by alternately coating SF and APTES on the template of polystyrene (PS) nanospheres by electrostatic attraction. SF nanocapsules were obtained after removal of the PS templates. Zeta potential analysis proved LBL assembly was indeed driven by the interaction between negative charge of SF and positive charge of APTES. Fluorescence images and electric microscope images indicated that SF nanocapsules had a hollow and stable structure with diameter at nearly 250 nm. The highest encapsulation rate of DOX or Ce6 were up to 80% and 90%, respectively, indicating SF nanocapsules have a high loading capability for both cationic and anionic drugs. In vitro cell experiments proved the biocompatibility of SF nanocapsules and their burst drug release in response to acidic environment. Furthermore, chemotherapy and photodynamic therapy proved SF nanocapsules loaded with DOX or Ce6 had significant inhibition on tumor cells. Our results suggested that this LBL technique is a facile method for polymers with negative charge to fabricate nanocapsules for antitumor drug carrier.
研究领域:
生物材料学
·
药物递送系统
·
肿瘤治疗
✓ 已验证
DOI
高影响因子
Polymers
·
2021
·Vol. 14
·期 1
·108
·
IF 4.329
Bo-Xiang Wang,Jia Li,De-Hong Cheng,Yan-Hua Lu,Li Liu
Antheraea pernyisilk fibroin (ASF)-based nanofibers have wide potential for biomaterial applications due to superior biocompatibility. It is not clear whether the ASF-based nanofibers scaffold can be used as an in vitro cancer cell culture platform. In the current study, we fabricated novel ASF-based thermoresponsive hydrogel nanofibers by aqueous electrospinning for colon cancer (LoVo) cells culture. ASF was reacted with allyl glycidyl ether (AGE) for the preparation of allyl silk fibroin (ASF-AGE), which provided the possibility of copolymerization with allyl monomer. The investigation of ASF-AGE structure by1H NMR revealed that reactive allyl groups were successfully linked with ASF. ASF-based thermoresponsive hydrogel nanofibers (p (ASF-AGE-NIPAAm)) were successfully manufactured by aqueous electrospinning with the polymerization of ASF and N-isopropylacrylamide (NIPAAm). The p (ASF-AGE-NIPAAm) spinning solution showed good spinnability with the increase of polymerization time, and uniform nanofibers were formed at the polymerization time of 360 min. The obtained hydrogel nanofibers exhibited good thermoresponsive that the LCST was similar with PNIPAAm at about 32 °C, and good degradability in protease XIV PBS solution. In addition, the cytocompatibility of colon cancer (LoVo) cells cultured in hydrogel nanofibers was assessed. It was demonstrated that LoVo cells grown on hydrogel nanofibers showed improved cell adhesion, proliferation, and viability than those on hydrogel. The results suggest that the p (ASF-AGE-NIPAAm) hydrogel nanofibers have potential application in LoVo cells culture in vitro. This study demonstrates the feasibility of fabricating ASF-based nanofibers to culture LoVo cancer cells that can potentially be used as an in vitro cancer cell culture platform.Keywords:Antheraea pernyisilk fibroin;nanofibers;thermoresponsive;cancer cell;in vitro culture
研究领域:
生物材料学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
FIBERS AND POLYMERS
·
2022
·Vol. 23
·期 8
·2225-2233
·
IF 2.347
Wu Leilei,Bao Xueming,Ren Yiwen,Wang Ping,Zhou Man,Yu Yuanyuan,Wang Qiang
Generally, Bombyx mori silk dyeing is performed using acid dyes. However, the color fastness is always unsatisfactory due to the weak binding forces under neutral conditions. In the present work, graft polymerization of α-lipoic acid (ALA) onto silk fibers was carried out using a two-step method. Briefly, ALA was grafted onto the silk fibers, followed by a reduction-oxidation process to form poly(α-lipoic acid) (PALA) on the fiber surfaces, aiming at improving the electronegativity of fiber surface and realizing cationic dyeing. The results implied that the combined treatments promoted the intermolecular crosslinking between fibroin chains, endowing the silk fiber with low zeta potential simultaneously. A thin sedimentary layer on the fiber surfaces was observed for the PALA-deposited silk fibers, companying with a significant increase in the content of sulfur and O/N ratio, which verified the successful graft polymerization. The modified silk fabric exhibited improved dyeability, and the dyeing depth and color fastness was noticeably higher compared to that of the untreated after cationic dyeing. Meanwhile, the newly-built intermolecular disulfide crosslinks imparted a detectable anti-wrinkle effect to the silk fabric. This study provides a facile strategy for modification of silk fabric with improved dyeing behaviors and physical properties.
研究领域:
材料科学
·
纺织工程
·
高分子化学
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产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2020
·Vol. 32
·期 34
·2002695
·
IF 27.398
Zongpu Xu,Mingrui Wu,Weiwei Gao,Hao Bai
Flexible and transparent substrates play a fundamental role as a mechanical support in advanced electronic devices. However, commonly used polymer films, such as polydimethylsiloxane, show low tear resistance because of their crack sensitivity. Herein, inspired by the excellent mechanical robustness of the skin and its fibrous structure, an epoxy-resin-based composite with a flat silk cocoon as a reinforcing fiber network is fabricated. With only 1 wt% of silk fiber, the tensile strength and modulus of the as-prepared composite film are considerably increased by 300% and 612% compared to those of pure resin, while still maintaining flexibility and transparency. More importantly, the composite shows remarkable tear resistance: without fracture after ≈30 000 tensile cycles. The potential application of such transparent composite films as mechanically robust substrates for flexible electronics is also demonstrated. In addition, this study represents a bioinspired strategy to construct high-performance functional composite materials.
研究领域:
材料科学
·
生物医学工程
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DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 7
·3558-3569
·
IF 3.049
Hao Xu,Wenhui Yi,Dongfan Li,Ping Zhang,Sweejiang Yoo,Lei Bai,Jin Hou,Xun Hou
Silkworm fibers have attracted widespread attention for their superb glossy texture and promising mechanical performance. The mechanical properties can be reinforced with carbon nanofillers, particularly carbon nanotubes (CNTs), depending on the CNT content in the silk fibers. In order to increase the CNT content, lignosulfonate (LGS) was used as a surfactant to ameliorate the CNT solubility, dispersibility, and biocompatibility. The resulting CNT/LGS nano-composite was further processed through an additional purification method to remove excess surfactant and enhance the CNT/LGS ratio. Then the purified biocompatible single and multiple-walled CNTs were fed to silkworms, leading to a large CNT content in the resulting silk fibers. Reinforced silk fibers were produced with a mechanical strength as high as 1.07 GPa and a strain of 16.8%. The toughness modulus is 1.69 times than that of the unpurified group. The CNT-embedded silk fibers were characterized via Raman spectrometry and thermogravimetric analysis (TGA), demonstrating that the CNT content in the silk fibers increased 1.5-fold in comparison to the unpurified group. The increased CNT content not only contributed to the self-assembly into buffering knots of silk fibers, but it also enhanced the conductivity of graphitized silk. Our coating and purification strategies provide a potential facile way to obtain natural silk fibers with high mechanical performance.
研究领域:
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）