ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
·
2018
·Vol. 99
·期 2
·e21499
·
IF 1.316
Lin Zhu,De-Qing Zou,Zuo-Qing Fan,Na Wang,Ying-Ying Bo,Yu-Qing Zhang,Guang Guo
In this study, a carboxymethyl chitosan derived from silkworm pupa (SP-carboxymethyl chitosan) was prepared. The physical characteristics of the SP chitin, chitosan, and carboxymethyl chitosan were analyzed. The scanning electron microscopy results showed that the surfaces of the samples from SP were more uneven, with more surface fractures compared with those of the reference substance (RS). Thermal analysis, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy analysis showed that the main molecular chain structures of SP samples and RSs had no substantial differences. However, the crystallinity and thermal decomposition temperature of the SP samples were lower compared with those of the RSs. All of these results provide a theoretical basis for the development of applications for the SP-carboxymethyl chitosan.
研究领域:
生物材料学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2021
·Vol. 194
·期 3
·1290-1302
·
IF 2.926
Ji Yingrui,Xu Long,Xu Qingqing,Liu Xuan,Lin Sen,Liao Sentai,Wang Weifei,Lan Dongming
More and more industries demand environmental friendliness. Silkworm pupae oil (SPO), extracted from the desilked silkworm pupae, can serve as a promising substrate alternative to use in plasticization. This study aimed to prepare epoxidized silkworm pupae oil (ESPO) and investigate their effects on the thermal stability and plasticization of polyvinyl chloride (PVC) films. A chemo-enzymatic method of ESPO was developed in the presence of Lipase SMG1-F278N and H 2 O 2 in natural deep eutectic solvents (DESs). Lipase SMG1-F278N could initiate the epoxidation reaction effectively at room temperature with a negligible loss of activities 10 batches. A maximum oxirane value of 6.94% was obtained. The formation of oxirane ring in ESPO was confirmed by FTIR and 13 C NMR spectra. Moreover, ESPO showed a better thermal stability and lower freezing point than epoxidized soybean oil (ESO). It was demonstrated that ESPO had a good frost resistance. In addition, ESPO showed a significantly improved plasticizing effect on flexible polyvinyl chloride (PVC). Compared with ESO, ESPO could increase the tensile elongation at break effectively. A significantly lower migration rate of plasticizer was observed in PVC plasticized with ESPO.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Hua Li,Yu Liu,Nidthaya Seephua,Chuenjit Prakitchaiwattana,Rui-Xin Liu,Ju-Sheng Zheng,Sirithon Siriamornpun
To improve the nutritional value and biofunctionalities of traditional rice noodles, this study focused on the effects of incorporating cricket powder (CP) and silkworm pupae powder (SP) on the protein content and digestibility, starch digestibility, and antioxidant potential, as well as the sensory and cooking qualities of rice noodles. Compared to the control (4.96 % and 63.25 %), CP and SP significantly increased the protein content (12.06 %–25.85 %) and in vitro digestibility (71.61 %–90.58 %) ( p < 0.05). CP and SP also considerably reduced the estimated glycemic index from 70.36 to 53.47, which may be partly related to the binding of more protein and lipid, as revealed by Fourier-transform infrared spectroscopy. Nevertheless, scanning electron microscopy showed a loose and porous noodle structure, which contributed to higher cooking loss, reduced cohesiveness, and lower tensile strength. Overall, these findings suggest that edible insect powders are promising ingredients for developing functional foods with enhanced nutritional properties.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Long Liu,Yanan Wei,Qing Chang,Huaju Sun,Kungang Chai,Zuqiang Huang,Zhenxia Zhao,Zhongxing Zhao
A novel, moderately hydrophilic peptide (RYL) with high ACE-inhibitory activity was screened ultrafast via a concept of waste conversion using waste. This novel peptide was screened from silkworm pupa using an Fe-doped porous biocarbon (FL/Z-SE) derived from silkworm excrement. FL/Z-SE possessed magnetic properties and specific selection for peptides due to Fe’s dual functions. The selected RYL, which has moderate hydrophilicity (LogP = −0.22), exhibited a comparatively high ACE-inhibitory activity (IC50 = 3.31 ± 0.11 μM). The inhibitory kinetics and docking-simulation results show that, as a competitive ACE inhibitor, RYL formed five hydrogen bonds with the ACE residues in the S1 and S2 pockets. In this work, both the screening carbon material and the selected ACE-inhibitory peptide were derived from agricultural waste (silkworm excrement and pupa), which offers a new way of thinking about the development of advanced uses of the silkworm byproducts and wastes.
研究领域:
生物技术
·
环境科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Wenting He,Hong Huang,Jia He,Sidra Subhan,Yi Peng,Meiyun Huang,Hui He,Ying Tang,Zhongxing Zhao
Antioxidant peptides from edible protein sources have attracted great attention due to their high activity, easy adsorption and low toxicity. The purpose of this study was to develop a new MOF material with specific recognition and capture ability to achieve the aim of highly efficient and selective antioxidant peptides from silkworm pupa protein hydrolysate-N (SPPH-N). Firstly, Histidine (His) widely presented in antioxidant peptides was used to in-suit construct the modified ZIF-8 material (ZIF-His) with imprinted defective mesopores, based on “molecular imprinting strategy”. Then, it was applied to extract antioxidant peptides from SPPH-N. ZIF-His exhibited high adsorption uptake for antioxidant peptides and especially presented specific adsorption property for peptides contained His, tryptophan, phenylalanine and tyrosine. Three peptides with the highest antioxidant activity (FKVPNMY, AVNMVPFPR and VNMVPFPR) from ZIF-His eluent were identified by Nano-LC-MS/MS. These peptides were synthesized and presented the excellent free radicals scavenging activity and the Ferric reducing power.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2025
·
IF 4
Zhixuan Xie,Luping Wang,Binqi Wang,Xueli Man,Yifei Wang,Taian Jin,Jiahui Ma,Eman Shawky,Samah Elsohafy,Tingfang Ma,Jianhua Li,Juan Jin,Qiang He,Jingkui Tian
Diabetic kidney disease (DKD), a prevalent microvascular complication affecting over 40 % of diabetes patients globally, imposes substantial burdens on healthcare systems through its dual impact on renal function deterioration and cardiovascular mortality. Silkworm pupae (SWP) is an ancient functional food documented in Chinese Materia Medica and Southeast Asian dietary practices, has recently emerged as a research focus due to its unique composition of chitin-derived peptides, antioxidant flavonoids, and angiotensin-converting enzyme inhibitory components. Building upon existing evidence of SWP's anti-inflammatory properties, this investigation evaluated the renoprotective effects of SWP in two representative stages—C2 (the fragile and soft stage) and C4 (nearly eclosion). The results indicated that SWP alleviated renal dysfunction and renal fibrosis through inhibiting apoptosis in DM mice. Notably, C2-stage extracts outperformed C4 counterparts in restoring podocyte integrity. These findings position SWP as a nutraceutical intervention for DKD management.
研究领域:
医学
·
营养学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 4
·期 3
·387-393
·
IF 4.726
Ji Feng,Wen-Jing Wang,Xin Hai,Yong-Liang Yu,Jian-Hua Wang
Carbon dots (CDs) with a high quantum yield have been synthesized by a facile and green one-pot approach under microwaves with silkworm chrysalis (SC) as the natural carbon source, without using any other chemicals/reagents. The morphology and optical properties of the resultant CDs are characterized by TEM, XRD, FT-IR, XPS, UV-vis and photoluminescence (PL). The SC-CDs have an average size of 19 nm, and contain C, O and N with relative contents of ca. 71.32%, 22.96% and 5.72%, respectively. A significant emission at 420 nm at an excitation wavelength of 350 nm is recorded, resulting in a quantum yield of 46% with quinine sulfate (quantum yield 54%) as a reference. In addition to excellent solubility and stability in aqueous medium, the SC-CDs exhibit excitation-dependent photoluminescence with a large Stokes shift of 70 nm. It is further demonstrated that the SC-CDs exhibit a low cytotoxicity at a higher concentration of 15 mg mL−1 and they are able to display bright blue, green and red colors under an inverted fluorescence microscope during cell imaging experiments, showing their vast potential in bioimaging.
研究领域:
材料科学
·
纳米技术
·
生物成像
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 8
·5733-5741
·
IF 2.6
Jinyue Zheng,Yvxin Ding,Dan Han,Caichun Liang,Jun-Hua Shao
Summary The purpose of this experiment was to explore the improvement of tussah pupa powder on the deterioration of pork batter emulsion gel by using proteomics, distribution of moisture, rheological properties and surface hydrophobicity. The results showed that the myosin decomposed as the heating temperature increased; as the increase of the addition proportion of tussah pupa powder at each sterilisation temperature point, compared to the control group, the pH of a pork batter emulsified gel showed a significant increased trend ( P < 0.05); the low molecular weight proteins or formed oligomers of tussah pupa powder could be used as fillers to participate in the formation process of gel network; the vitellogenin of tussah pupa powder could form a ‘skeleton’ in the pork batter system; as the sterilisation temperature at 100 °C (30 min) and 110 °C (30 min), the surface hydrophobicity was the largest as the addition amount 2%. In short, the tussah pupae powder could improve the deterioration characteristics of the pork batter emulsified gel by influencing the pH, surface hydrophobicity and the formation of gel network of pork batter system. This research provided theoretical support for improving the process property of the meat products.
研究领域:
食品科学
·
蛋白质组学
·
肉类加工技术
✓ 已验证
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2015
·
IF 3.088
Lin Zhu,Yu-Qing Zhang
N,O-Carboxymethyl chitosan (NOCC) can prevent postsurgical adhesion formation. Here, we described the preparation of a novel silkworm pupa NOCC and its effects on the prevention of postoperative adhesion in a rat cecal abrasion model. The degree of deacetylation (DDA) of silkworm pupa chitosan was only 49.87 ± 0.86%; regardless, it was used as the raw material to construct the novel silkworm pupa NOCC, which had a weaker crystallinity than the NOCC standard. Sixty male Sprague–Dawley rats were divided into three groups and treated as follows: 0.9% normal saline solution as a negative control, medical anti-adhesion gel as a positive control and the silkworm pupa NOCC anti-adhesion solution. Two and three weeks after surgery, the animals were killed and the adhesion formation was scored. The silkworm pupa NOCC solution significantly decreased the levels of WBC, TNF-α, IL-1β, IL-2, IL-6 and IL-8 but had no effect on IL-4. Additionally, a lower level of TGF-β 1 expression was found in the silkworm pupa NOCC group, and significantly less collagen ( P < 0.01) and fewer inflammatory cells and fibroblasts were detected in the animals of this group. These results suggested that the novel NOCC from silkworm pupa using the method described here have potential applications in the prevention of postoperative intestinal adhesion.
研究领域:
生物医学工程
·
药理学
·
外科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Xuan Zhu,Yi-Fan Zhao,Hong-Jian Wen,Yu Lu,Shuai You,Richard Ansah Herman,Jun Wang
Silkworm pupae, by-product of sericulture industry, is massively discarded. The degradation rate of silkworm pupae protein is critical to further employment, which reduces the impact of waste on the environment. Herein, magnetic Janus mesoporous silica nanoparticles immobilized proteinase K mutant T206M and Mucor circinelloides aspartic protease were employed in the co-degradation. The thermostability of T206M improved by enhancing structural rigidity ( t 1/2 by 30 min and T 50 by 5 °C), prompting the degradation efficiency. At 65 °C and pH 7, degradation rate reached the highest of 61.7%, which improved by 26% compared with single free protease degradation. Besides, the immobilized protease is easy to separate and reuse, which maintains 50% activity after 10 recycles. Therefore, immobilized protease co-degradation was first applied to the development and utilization of silkworm pupae resulting in the release of promising antioxidant properties and reduces the environmental impact by utilizing a natural and renewable resource.
研究领域:
生物技术
·
环境科学
·
蛋白质工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Kexin Yi,Shiyuan Miao,Bixing Yang,Sijie Li,Yiwen Hu,Yujie Lu
As a naturally derived biomacromolecule, chitosan is widely utilized in food preservation. However, the chitosan derived from silkworm pupae and its application in food preservation are relatively unexplored. To obtain chitosan with enhanced preservation properties, silkworm pupae chitosan was extracted using dual-frequency ultrasound-assisted chemical and enzymatic methods, followed by a comparative analysis of their physicochemical properties, biological activity, and preservation efficacy on strawberries. Compared with the commercial chitosan, the yield, physicochemical properties, and biological activity of silkworm pupae chitosan using the dual-frequency ultrasound-assisted enzymatic method were increased, exhibiting a solubility of 98.1 % and a deacetylation degree of 99.12 %. The silkworm pupae chitosan demonstrated the highest antioxidant activity and effective microbial inhibition at the concentration of 1 % (w/w). When applied as a coating solution for strawberry preservation, this chitosan significantly reduced weight loss, titratable acid degradation, and polyphenol consumption. Furthermore, it decreased the decay rate by 50 % and extended shelf life by two-fold compared to untreated samples. The dual-frequency ultrasound-assisted enzymatic method provides an eco-friendly approach for chitosan extraction, with silkworm pupae-derived chitosan showing excellent potential as a biodegradable food preservative.
研究领域:
食品科学
·
生物技术
·
高分子科学
·
农产品保鲜
·
应用微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sustainable Chemistry and Pharmacy
·
2022
·
IF 5.464
Cheng-Kun Wu,Jin-Zheng Wang,Cheng-Hai Yan,Can-Yang Shi,Huan Chen,Sheng Sheng,Shuai You,Fu-An Wu,Jun Wang
Silkworm pupae-derived structured lipids are expected to promote the absorption of unsaturated fatty acids, however, low efficiency of bioprocess and low reusability of biocatalyst limit the corresponding biocatalysis technology . Thus, a novel strategy combining magnetic response immobilized lipase and continuous-flow microchannel reactor was proposed to modify silkworm pupae oil. The lipase from Aspergillus niger was immobilized on the surface of chitosan cross-linked magnetic nanoparticles , with increased esterification activity from 151.51 to 418.10 U/g due to the hydrophobic microenvironment. In the microchannel, the enzyme activity could be increased to 2371.3 U/g because of the strengthening effect of the microfluidic field. Furthermore, the employment of chitosan cross-linked magnetic nanoparticles leads to good reusability, and the remaining activity could stay above 90% after 13 cycles. Moreover, the immobilized lipase could be removed and loaded easily due to the employment of magnetically responsive nanoparticles. In addition, 2 min was required to rearrange the unsaturated fatty acids, increasing the relative content of sn -1.3 unsaturated fatty acids from 59.91% to 74.8% and sn -2 palmitic acid from 2.69% to 18.88%, which indicated the improved nutritional value of modified silkworm pupae oil. Therefore, the combination of microfluidic field and hydrophobic microenvironment formed by the chitosan layer on the surface of the nanoparticles effectively strengthens the biocatalysis in a solvent-free system, while the developed strategy provides a convenient biocatalysis strategy for the quality improvement of edible insect oil.
研究领域:
生物技术
·
化学工程
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）