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Journal of Food Measurement and Characterization · 2022 ·Vol. 16 ·期 6 ·4470-4485 · IF 3.006

Physiochemical and thermal characterisation of faba bean starch

Nilsson Klara,Sandström Corine,Özeren Hüsamettin Deniz,Vilaplana Francisco,Hedenqvist Mikael,Langton Maud

The structure and physicochemical properties of starch isolated from the cotyledon and hull of faba beans and from wheat (as reference) were examined using 16 different methods. The amylose content in faba bean cotyledon and hull starch was 32% and 36%, respectively, and that in wheat starch was 21%. The faba bean cotyledon and hull starch were structurally alike both displaying C-polymorphic pattern, a similar degree of branching and similar branch chain length distributions. Wheat starch had a significantly greater prevalence of short amylopectin chains (DP < 12) and a higher degree of branching. Granules in both faba bean starches exhibited surface cracks and were more homogenous in size than the smoother wheat starch granules. Gelatinisation temperature was higher for the faba bean starches, likely as an effect of high amylose content and longer starch chains delaying granular swelling. Cotyledon starch produced pastes with the highest viscosities in all rheological measurements, probably owing to larger granules. Higher prevalence of lipids and resistant starch reduced the viscosity values for hull starch. For all starches, viscosity increased at faster heating rates. During the rheological analyses, the samples were exposed to different instruments, heating rates and temperatures ranges, differing from standard rheological procedures, which could help predict how different processing techniques effect the final starch textures. Graphical abstract

研究领域: 食品科学 · 植物化学 · 生物材料
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PLANT CELL REPORTS · 2024 ·Vol. 43 ·期 4 ·1-15 · IF 5.3

Benzoxazinoids secreted by wheat root weaken the pathogenicity of Fusarium oxysporum f. sp. fabae by inhibiting linoleic acid and nucleotide metabolisms

Ma Guanglei,Hu Bijie,Yang Siyin,Cen Zixuan,Zheng Yiran,Dong Yan

Key message The regulatory action of BXs secreted by wheat on the pathogenicity of FOF causing Fusarium wilt in faba bean were analyzed. DIMBOA and MBOA weakened the pathogenicity of FOF. Abstract A large number of pathogenic bacteria in continuous cropping soil infect faba bean plants, leading to the occurrence of wilt disease, which restricts their production. Faba bean–wheat intercropping is often used to alleviate this disease. This study investigates the effect of benzoxazinoids (BXs) secreted by wheat root on the pathogenicity of Fusarium oxysporum f. sp. Fabae (FOF) and underlying molecular mechanisms. The effects of DIMBOA(2,4-dihydroxy-7-methoxy-1,4-benzoxazine-4-one) and MBOA(6-methoxybenzoxazolin-2-one) on the activity of cell-wall-degrading enzymes in FOF(cellulase, pectinase, amylase, and protease), FOF Toxin (fusaric acid, FA) content were investigated through indoor culture experiments. The effect of BXs on the metabolic level of FOF was analyzed by metabonomics to explore the ecological function of benzoxazines intercropping control of Fusarium wilt in faba bean. The results show that the Exogenous addition of DIMBOA and MBOA decreased the activity of plant-cell-wall-degrading enzymes and fusaric acid content and significantly weakened the pathogenicity of FOF. DIMBOA and MBOA significantly inhibited the pathogenicity of FOF, and metabolome analysis showed that DIMBOA and MBOA reduced the pathogenicity of FOF by down-regulating related pathways such as nucleotide metabolism and linoleic acid metabolism, thus effectively controlling the occurrence of Fusarium wilt in faba bean.

研究领域: 植物学 · 微生物学 · 分子生物学
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Animal Nutrition · 2025 · IF 7.5

Mechanisms of hepatic dysfunction in Nile Tilapia (Oreochromis Niloticus) fed a figh-fava bean (Vicia faba L.) diet

Xinyao Zhang,Ke Cheng,Lixue Dong,Dexing Zhu,Qingyong Li,Zhongbao Guo,Yongju Luo,Apeng Liu,Juan Tian,Di Peng,Jianmin Zhang,Yangyang Liu,Hua Wen,Ming Jiang

Fava bean is currently utilized as a specialized feed ingredient to enhance the muscle texture of fish. When incorporated at appropriate levels, fava beans can improve fish muscle crispy texture. However, high dietary levels of fava beans may lead to liver damage in fish, although the specific mechanisms behind this effect remain unclear. In this study, 600 genetic improvement of farmed tilapia (GIFT; Oreochromis niloticus ) of uniform size (initial body weight 552.3 ± 10.12 g) and healthy appearance were randomly divided into four groups, each with three replicates of 50 fishes. The fish diets contained different levels of fava beans (0, 50%, 60%, and 70%), designated as D0, D50, D60, and D70, respectively, over a ten-week feeding experiment. A comprehensive analysis of the impact of fava beans on muscle texture and liver health in GIFT was performed. The results showed that 50%, 60%, and 70% dietary fava beans had no significant effect on the growth performance of GIFT ( P > 0.05), but 70% dietary fava beans increased feed conversion ratio (FCR) ( P = 0.022). Consumption of fava beans at levels of 50%, 60%, and 70% in the diet altered GIFT muscle texture ( P < 0.05), and adversely effected serum biochemistry and liver health ( P < 0.05). Non-targeted metabolome analysis of the liver revealed that dietary fava beans may cause irreversible damage to liver cells by disrupting the DNA methylation process ( P < 0.05). This finding enhances the understanding of the mechanisms behind the negative impact of fava bean consumption on liver function. It is crucial to investigate potential strategies to mitigate these adverse effects while maintaining the desired muscle quality when fava beans are used in aquatic feed.

CAS / MDL: 886-86-2
研究领域: 水产养殖学 · 分子生物学 · 营养学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2025 · IF 8.5

Study on structure, properties and formation mechanism of cassava starch-faba bean protein heat-induced gel

Jianyu Huang,Cai Ying,Xiaoqiong Li,Jian Kuang,Jianqiang Li,Tao Huang,Jinjun Li

In this experiment, the effects of different concentrations of cassava starch (CS) on the gel behavior of faba bean protein (FBP) were studied, focusing on the structural characteristics, gel characteristics and physical and chemical characteristics of the gel system. Specifically, with the increase of CS concentration from 4 % to 12 %, the morphology of the sample changed from fluid to gel solid. From the molecular structure, different concentrations of CS affected the secondary and tertiary structures of FBP protein, which made aromatic amino acids move to the surface of protein and promoted the transformation from α-helix to β-sheet. In addition, free sulfhydryl groups are converted into disulfide bonds, which increases the number of hydrogen bonds in the system. Microscopically, high temperature treatment leads to the cracking of CS and FBP structures, which enhances the noncovalent interactions between them, and forms a compact and smaller pore three-dimensional network structure, providing more channels for external moisture to transfer to the inside. From the gel characteristics, the water holding capacity, viscoelasticity and mechanical behavior of the composite gel were improved when the concentration of CS was 6 %-12 %. This work provides reference for the application of cassava starch-faba bean protein gel in specific food and medicine fields.

研究领域: 食品科学 · 生物化学
✓ 已验证 DOI PubMed 高影响因子
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Foods · 2020 ·Vol. 9 ·期 12 ·1816 · IF 4.092

The Composition and Antioxidant Activity of Bound Phenolics in Three Legumes, and Their Metabolism and Bioaccessibility of Gastrointestinal Tract

Liuying Zhu,Wenting Li,Zeyuan Deng,Hongyan Li,Bing Zhang

The composition and antioxidant activity of bound phenolics in three legumes (soybean, vicia faba, and kidney bean), and their metabolism and bioaccessibility in the gastrointestinal tract were investigated in this study. The total phenolic content, total flavonoid content, and antioxidant activities (ABTS and FRAP) were evaluated. The phytochemical compositions of the three legumes after acid/alkaline hydrolysis, simulated gastrointestinal digestion, and colonic fermentation were identified and quantified by UPLC-ESI-QTOF-MS/MS and HPLC-ESI-QqQ-MS/MS. The results showed that the three legumes were rich in bound phenolic compounds, and possessed a strong antioxidant activity; among which kidney bean showed a higher bound flavonoid content and antioxidant activity than the other two legumes. Alkaline hydrolysis allowed a more thorough extraction of the bound phenolics of the three legumes than acid hydrolysis. The released contents of bound phenolics were extremely low in in vitro digestion, whereas colonic fermentation favored the release of more phenolic compounds. Kidney bean, which presented the highest bound flavonoid content and antioxidant activity, had the lowest bioaccessibility. Our study provides a wider insight into the constituents and bioavailability of bound phenolic compounds in the three legumes.Keywords:soybean;vicia faba;kidney bean;acid/alkaline hydrolysis;in vitro digestion;colonic fermentation

研究领域: 食品科学 · 植物化学 · 营养学
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CELLULAR POLYMERS · 2014 · IF 0.6

Preparation and Characterization of Biopolyols and Polyurethane Foams from Liquefied Faba Bean Stalk

Wenming Zhang,Ling Li,Qing Li,Xiaowei Li,Yucang Zhang

In this study, Faba bean stalk was liquefied for the production of biopolyols and polyurethane (PU) foams. The influences of various reaction conditions on biopolyols and PU foams were examined. Furthermore, the property relationships between biopolyols and PU foams were investigated. Biopolyols produced under the liquefaction conditions of 160°C, 90 min, 3% sulfuric acid loading, and 20% Faba bean stalk loading showed promising material properties (i.e. hydroxyl number, 496.1 mg KOH/g, acid number, 17.9 mg KOH/g, and viscosity, 1.1 Pa.s). PU foams produced from promising biopolyols showed compressive strength of 205 kPa and density of 0.035 g/cm3. The results suggested that in addition to density, the compressive strength of PU foams might also be affected by factors such as biomass residues or the chemical structures of biopolyols. The biopolyols and PU foams produced showed material properties comparable to those of conventional PU foams.

研究领域: 材料科学 · 生物化学
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FISH PHYSIOLOGY AND BIOCHEMISTRY · 2024 ·Vol. 50 ·期 3 ·1157-1169 · IF 2.5

Effect of faba bean Vicia faba L. water/alcohol extract on growth performance, antioxidant capacity, textural properties, and collagen deposition in the swim bladder of juvenile Nibea coibor

Guo Haoji,Liu Guoquan,Tian Mengdu,Liu Chunsheng,Zhang Haoran,Wang Shuqi,Wen Xiaobo,Lin Fan

Faba bean has gained attention as a cost-effective protein source with the potential to enhance product quality (texture properties, collagen content, etc.) in fish. However, its anti-nutrition factor, high feed conversion ratio, poor growth performance, etc. limit the widely application as a dietary source, especially in carnivorous fish. The water or alcohol extract of faba bean might resolve the problem. In this study, the juvenile Nibea coibor , known for their high-protein, large-sized, and high-grade swim bladder, were fed with seven isoproteic and isolipid experimental diets with the additive of faba bean water extract (1.25%, 2.5%, and 5%) or faba bean alcohol extract (0.9%, 1.8%, and 3.6%), with a control group without faba bean extract. After the 10-week feeding trail, the growth, antioxidant capacity, textural properties, and collagen deposition of the swim bladder were analyzed. Results showed that the 1.25% faba bean water extract group could significantly promote growth, textural quality of the swim bladder, and have beneficial effects on antioxidant response of fish. Conversely, dietary supplementation of faba bean alcohol extract resulted in reduced growth performance in a dose-dependent manner. Furthermore, fish fed diet with 1.25% faba bean water extract exhibited increased collagen content and upregulated collagen-related gene expression in the swim bladder, which was consistent with the Masson stain analysis for collagen fiber. Our results suggested that the anti-nutrient factor and bioactive component of faba bean may mainly be enriched in alcohol extract and water extract of faba bean, respectively. Besides, the appropriate addition of water extract of faba bean may improve the texture quality of the swim bladder by promoting collagen deposition. This study would provide a theoretical basis for the formulated diets with faba bean extract to promote product quality of marine fish.

研究领域: 水产养殖学 · 营养学 · 食品科学
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International Journal of Agronomy · 2020 · IF 0

Faba Bean Gall (Olpidium viciae K.) as a Priority Biosecurity Threat for Producing Faba Bean in Ethiopia: Current Status and Future Perspectives

Birhanu Kahsay Meresa,Hailay Mehari Gebremedhin

The diploid cool-season legume species, faba bean (Vicia faba L.), is one of the vital pulses for the people in the world as it maintains the sustainability of agriculture and provides nutrient-rich grains. Biotic and abiotic stresses are, however, challenging the faba bean production in many countries. The foliar diseases of faba bean are among the major constraints for the production and productivity of faba bean. Recently, a new foliar disease “Faba bean gall” caused by plant debris and soil-borne pathogen (Olpidium viciae K.) is rapidly spreading and causing high yield losses in Ethiopia. This review paper presents the distribution, impact, epidemiology, and biology of faba bean gall pathogen and principally illustrates how the application and investigation of the various diseases’ management approaches such as avoiding crop residues, crop rotation, intercropping, use of elicitors to induce host resistance, use of resistant genotypes, bioagents, compost teas, plant extracts, and lastly use of chemical fungicides could be important to control the faba bean gall disease underlying in faba bean.

研究领域: 农业 · 植物病理学 · 作物科学 · 生物安全
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PeerJ · 2023 · IF 2.7

RNA-Seq and genetic diversity analysis of faba bean (Vicia faba L.) varieties in China

Wanwei Hou,Xiaojuan Zhang,Yuling Liu,Yujiao Liu,Bai li Feng

Background Faba bean ( Vicia faba L) is one of the most important legumes in the world. However, there is relatively little genomic information available for this species owing to its large genome. The lack of data impedes the discovery of molecular markers and subsequent genetic research in faba bean. The objective of this study was to analyze the faba bean transcriptome, and to develop simple sequence repeat (SSR) markers to determine the genetic diversity of 226 faba bean varieties derived from different regions in China. Methods Faba bean varieties with different phenotype were used in transcriptome analysis. The functions of the unigenes were analyzed using various database. SSR markers were developed and the polymorphic markers were selected to conduct genetic diversity analysis. Results A total of 92.43 Gb of sequencing data was obtained in this study, and 133,487 unigene sequences with a total length of 178,152,541 bp were assembled. A total of 5,200 SSR markers were developed on the basis of RNA-Seq analysis. Then, 200 SSR markers were used to evaluate polymorphisms. In total, 103 (51.5%) SSR markers showed significant and repeatable bands between different faba bean varieties. Clustering analysis revealed that 226 faba bean materials were divided into five groups. Genetic diversity analysis revealed that the relationship between different faba beans in China was related, especially in the same region. These results provided a valuable data resource for annotating genes to different categories and developing SSR markers.

研究领域: 植物学 · 遗传学 · 分子生物学
✓ 已验证 DOI PubMed 高影响因子
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Food Bioscience · 2024 · IF 4.8

Construction and application of a synthetic microbial community in reduced salinity fermentation of raw-materials based broad bean paste

Chengtuo Niu,Xianlei Xing,Wenjun Zuo,Zongchi Zuo,Fuming Liu,Chunfeng Liu,Qi Li

Raw-materials based broad bean paste (rmBBP) is popular among consumers. Though high salinity can select functional microbial community for fermentation, it also impaired the fermentation efficiency of rmBBP and unfavored human health. Therefore, this study aimed to construct a synthetic microbial community based on key functional microbes for reduced salt rmBBP fermentation based on the elucidation of the microbial community succession during fermentation. Based on the tracked determination and multivariate statistical analysis of the physicochemical parameters, metabolites and the structure of microbial community, salinity and microbial interactions were key drivers for the succession of microbial community. Aspergillus , Staphylococcus , Weissella and Tetragenococcus were predicted to play key roles during fermentation. After that, core species were isolated from rmBBP mash and their salinity tolerance and metabolic characteristics were evaluated. Finally, a synthetic microbial community ( Aspergillus oryzae , Staphylococcus carnosus , Tetragenococcus halophilus and Weissella confusa ) was constructed and applied in reduced-salt rmBBP fermentation. The bioaugmentation contributed to the accumulation of volatile metabolites while effectively maintaining the acidity during rmBBP fermentation when salinity was reduced from 12% to 10%. In general, this study developed a synthetic microbial community with desired characteristics for successful reduced-salinity rmBBP fermentation.

研究领域: 食品科学 · 微生物学
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Journal of Biobased Materials and Bioenergy · 2017 ·Vol. 11 ·期 5 ·505-509 · IF 0.975

Comparative Study on Genotoxicity of Nano-Zinc Oxide and Bulk-Zinc Oxide Based on Micronucleus Test Technique of V. faba. Root Tip Cells

Pan Shaobing,Qiu Fengxian,Ping Zhang,Geng Rong,Xu Xueqin,Huang Tongxing,Ma Yichen,Yang Jiahui,Li Houzhi

Objectives: This paper focuses on comparing the micronucleus effect and evaluating genotoxicity of nano-zinc oxide and bulk-zinc oxide based on V. faba. root tip cells. Methods: The different concentration of nano-ZnO and bulk-ZnO suspensions are used as mutagens, meanwhile, the paper calculates the micronucleus permillage of V. faba. root tip cells exposed by different treatment liquid. Results: 1. Both nano-ZnO and bulk-ZnO suspensions can induce higher micronucleus permillage. The micronucleus permillage induced by different concentration of nano-ZnO and bulk-ZnO are all higher than the negative control group. The significance comparison of micronucleus permillage between bulk-ZnO and negative control group is significant (p < 0.05) and the significance comparison between nano-ZnO and negative control group is extremely significant (p < 0.01), both of difference comparison have statistical significance. 2. The micronucleus permillage which is induced by nano-ZnO is higher than bulk-ZnO on the equal concentration. The significance comparison of them is extremely significant (p < 0.01), the comparison has statistical significance. The genotoxicity to V. faba. root tip cells of nano-ZnO is higher than bulk-ZnO. 3. It has stronger operability and scientificalness that we use the optimized micronucleus test technique of V. faba. root tip cells to evaluate genotoxicity of manufactured nanomaterials.

研究领域: 遗传学 · 毒理学 · 环境科学
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INDUSTRIAL CROPS AND PRODUCTS · 2014 · IF 3.208

A novel anti-counterfeiting method: Application and decomposition of RB for broad bean seeds (Vicia faba L.)

Yixin Tian,Zhan Li,Fei He,Yajing Guan,Shuijin Zhu,Jin Hu

Combining with seed coating method, rhodamine B (RB), a kind of fluorescent compounds had been used in seed labeling as an anti-counterfeiting technology. Effects of broad bean seed coating with RB on physiological parameters and fluorescence performance in broad bean seedlings were performed. The prolonged fluorescence in vascular bundles of seedlings treated with RB could be used as a marker of seed anti-counterfeiting. The experiment showed that RB had no negative effect on seed germination, seedling growth, seedling protective enzyme activities, and the contents of malondialdehyde (MDA) and chlorophyll, after coating seeds with ratio 1   kg RB to 10–30   kg seeds. In addition, the RB adsorption by soil was accorded with the isothermal adsorption equations of Langmuir, which exhibited the linear correlation as 0.9959, the maximum adsorption as 1.716   mg/g, and the adsorption equilibrium constant as 0.042   L/mg. The soil adsorption of RB could be effectively degraded by UV/Fenton. Present research suggested that coating broad bean seeds with appropriate concentration RB were safe to seed germination and this technology was suitable for anti-counterfeiting application.

CAS / MDL: 81-88-9
研究领域: 农业 · 种子科学 · 生物技术 · 环境科学
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