JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 12
·4249-4259
·
IF 2.479
Song Gao,Dapeng Li,Hui Hong,Rui Shu,Hao Cheng,Yongkang Luo
The objective of this study was to compare the quality and nutritional attributes of snakehead ( Channa argus argus ) fillets (cultured in ponds and containers) after being heated (boiling, frying, and baking). The results revealed that there were few differences in quality (flavor, color, and texture) and nutritional attributes (content of amino acid, dipeptidyl peptidase IV (DPP-IV) and angiotensin I-converting enzyme (ACE) inhibition activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity) of snakehead fillets obtained from two cultured modes. Container-cultured samples exhibited more beneficial effects on flavor (more esters) and bioactivity (ACE inhibition and DPPH scavenging activity) than pond-cultured. Frying increased flavor attributes but decreased nutritional value of fish. Boiling was an effective heating method for increasing quality and nutritional attributes of container-cultured snakehead fish. Generally, the container-cultured mode was applicable for culturing snakehead without decreasing quality and nutritional attributes, which deserved more attention. Practical Application The study provided information about the differences of quality properties (flavor and texture) and nutritional attributes (bioactive functions of simulate-digested products) of pond-cultured and container-cultured snakehead fish after being heated. The results will provide some potential values for the application of container-cultured technology.
研究领域:
食品科学
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水产养殖
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2017
·Vol. 30
·期 9
·587-600
·
IF 1.32
Haili Wang,Chunxiang Yang,Jing Wang,Mo Chen,Donglei Luan,Li Li
Various studies have provided information on active packaging to prevent food putrefaction effectively . In this work, ethylene–vinyl alcohol copolymer (EVOH) films with geraniol (Ger) and α-terpilenol (Ter) were successfully prepared by extrusion. The mechanical, thermal, oxygen barrier and antimicrobial properties of the packaging films were characterized, and the application of the films in snakehead preservation was examined. Thermogravimetric analysis showed that the addition of Ger and Ter did not decrease the films properties but improved their oxygen barrier property and thermostability. Moreover, the incorporation of Ger and Ter inhibited the growth of Escherichia coli , Salmonella enterica and Listeria monocytogenes . Both of the films, EVOH/Ger (EVOH/Ger) and EVOH/Ter (EVOH/Ter), reduced Salmonella enterica by 2.36 ± 0.17 and 1.91 ± 0.06 log units, respectively. Meanwhile, the effects of EVOH/Ger and EVOH/Ter on quality assessment of snakehead slices were examined through sensory tests and analyses of biochemical indices during refrigerated storage (4°C ± 1°C) for 10 days. Sensory properties, total viable counts, total volatile basic nitrogen, thiobarbituric acid and pH values were evaluated. On eight day, compared with the control fish samples, the total viable counts and total volatile basic nitrogen values cut down 1.98 ± 0.02 log units and 11.3 ± 0.11 mgN/100 g, respectively, for fish samples packaged by EVOH/Ger. The active films containing 6% (wt) Ger and 6% (wt) Ter inhibited the growth of E. coli , S. enterica and L. monocytogenes and effectively extended shelf life by 4–5 days under cold-storage conditions compared with the control group. Copyright © 2017 John Wiley & Sons, Ltd.
研究领域:
食品科学
·
包装工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qingqing Yu,Hui Hong,Yueyue Liu,Abdul Razak Monto,Ruichang Gao,Yulong Bao
The pH buffering capacity is an important functionality of muscle proteins, and muscle foods are susceptible to being oxidized during storage and processing. In order to study the effect of oxidation on the pH buffering capacity of myofibrillar proteins, myofibrils extracted from snakehead fish ( Channa argus ) were oxidized with H 2 O 2 . Results showed that increased oxidation led to loss of free sulfhydryl groups, formation of carbonyl groups, increased surface hydrophobicity , and aggregation of myofibrillar proteins. In addition, there was a significant reduction in the content of histidine in oxidized myofibrillar proteins. The pH buffering capacity of myofibrillar proteins significantly decreased from 3.14 ± 0.03 mM H + /(mL × ΔpH) down to 2.55 ± 0.03 mM H + /(mL × ΔpH) after oxidation with 50 mM H 2 O 2 . Both oxidized myofibrillar proteins and histidine showed a high pH buffering capacity at pH near 5.8, which is the histidine pKa value. Here, we hypothesize that oxidation-induced changes in the pH buffering capacity of myofibrillar proteins were driven by oxidative modification of histidine and structural changes of myofibrillar proteins. The significance of this study to food industry may be the awareness that protein oxidation may affect pH through changes in buffering capacity. And the use of antioxidants, especially those targeting at histidine will be promising in addressing this issue.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Peng Shi,Yao Xie,Jun Mei,Jing Xie
BACKGROUND This study investigates the effect of microemulsions of 0.8% Ocimum basilicum essential oil (MOBO) on the cooking quality and shelf life of snakehead fillets using different impregnation methods (air environment treatment, vacuum impregnation, ultrasonic impregnation and vacuum impregnation with ultrasonic-assisted). Basil essential oil, recognized for its antimicrobial and antioxidant properties, is utilized in a microemulsion form to enhance its efficacy and application in food preservation. RESULTS The findings of the study demonstrated that MOBO markedly enhanced the cooking quality, flavor and overall acceptability of snakehead fillets. The incidence of microbial growth was markedly diminished in the MOBO-treated fillets in comparison to the control group. On day 9, a reduction in the total viable count of a minimum of 1.7 lg CFU/g was observed in comparison to the control group. The treated group exhibited an extended shelf life, with an increase of 3–9 days in comparison to the control group. However, there was a higher cooking weight loss in the treated group compared to the control group. This is from the findings regarding moisture content. The digestibility of the MOBO-treated fillets was enhanced by up to 4.13% in comparison to the control group, while the particle size was reduced by up to 40 nm. MOBO reduced the production of bitter amino acids and thiobarbituric acid. CONCLUSION Incorporating MOBO in the marination process effectively enhances the cooking quality and extends the shelf life of snakehead fillets. These results indicate the potential of basil essential oil microemulsion as a natural and efficient food preservation method, offering significant benefits for the food industry in terms of product quality and safety improvement. © 2024 Society of Chemical Industry.
研究领域:
食品科学
·
生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PeerJ
·
2021
·
IF 2.984
Xue Wang,Zhengjie Wu,Shengmei Wu,Xianxian Chen,Misbah Hanif,Shengzhou Zhang
Background The argus snakehead ( Ophiocephalus argus Cantor) is a highly nutritious, freshwater, cultured bony fish with a high economic value. The health of the fish is closely related to its blood cells, which are critical for oxygen transport, natural defense, and immunity. We investigated the morphometry, microstructure, and cytochemical characteristics of the peripheral blood cells of O. argus . Our results may provide the basic reference values needed to monitor the health of this fish for large-scale cultivation. Methods The number of blood cells in O. argus were counted on a hemocytometer and their size was measured using a micrometer under light microscope. The morphology and classification of the blood cells were studied using Wright’s staining and the cytochemical characteristics were studied using seven chemical stains including peroxidase (POX), Sudan black B (SBB), periodic acid-Schiff (PAS), acid phosphatase (ACP), alkaline phosphatase (ALP), chloroacetic acid AS-D naphthol esterase (AS-D), and α -naphthol acetate esterase ( α -NAE). Results The peripheral blood cells in O. argus can be classified as erythrocytes, leukocytes, and thrombocytes; of which, females had 2.9597 million/mm 3 , 88,400/mm 3 , and 43,600/mm 3 , respectively, and males had 3.0105 million/mm 3 , 105,500/mm 3 , and 34,000/mm 3 , respectively. Leukocytes consisted of neutrophils, monocytes, large lymphocytes, and small lymphocytes. Eosinophils and basophils were not found. Monocytes were the most numerous leukocytes identified, followed by neutrophils and small lymphocytes, while large lymphocytes were the least frequently identified. Cytochemical staining showed that erythrocytes were only positive for PAS staining. Neutrophils were strongly positive for POX, SBB, and ACP, and positive for all the other cytochemical stains. Monocytes were positive for PAS and α -NAE and were weakly positive for ACP and AS-D staining. Large lymphocytes were positive for PAS and were weakly positive for ALP, AS-D, and α -NAE staining. Small lymphocytes were positive for PAS and weakly positive for AS-D and α -NAE staining. Thrombocytes were positive for PAS and were weakly positive for ACP and AS-D, but negative for the remaining cytochemical stains. The morphology of peripheral blood cells in O. argus was generally similar to that of other fish species, while the cytochemical staining patterns showed clear species specificity.
研究领域:
水产养殖学
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鱼类生理学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE
·
2020
·
IF 3.225
Wenjing Zhang,Jingxin Wang,Guangming Zheng,Yi Yin,Xinping Zhu,Qi Shan,Yuanhao Yang,Lisha Ma,Lichun Li,Shugui Liu
Enrofloxacin (ENR) is effective in the prevention and treatment of bacterial infections caused by various fish pathogenic bacteria such as Aeromonas spp . , Vibrio spp . , Nocardia and Yersinia spp. The purpose of this work was to explore the pharmacokinetics (PK), tissue distribution, and depletion of ENR and its main metabolite, ciprofloxacin (CIP), in northern snakehead ( Channa argus ) reared at 25 °C during and after repeated oral dose of 10 mg/kg animal body weight per day for a total of 5 days. ENR and CIP concentrations were simultaneously determined in the plasma, liver, kidney , gill, and muscle with adhering skin by HPLC with fluorescence detection. Using a non-compartmental analysis, PK parameters were derived from the mean concentration versus time data of ENR and CIP. The results showed a slow absorption, long half-life and wide tissue distribution of ENR in the northern snakehead. The maximum concentrations of ENR in the plasma, muscle plus skin, liver, kidney, and gill tissues were observed at 3, 6, 6, 6, and 9 h post treatment, respectively, and the corresponding depletion half-lives (T 1/2z ) were 78.00, 83.24, 82.56, 126.07, and 68.70 h, respectively. The extent of ENR distribution into the tissues followed the decreasing order of liver > kidney > gill > plasma > muscle plus skin. The highest concentrations of both ENR and CIP were observed in the liver, suggesting that liver is the major site of ENR metabolism in northern snakehead. The C max /MIC and AUC 24 /MIC ratios obtained in the present study indicated that the ENR regimen employed would be effective in treatment of infections caused by susceptible strains with MIC values below 0.7 μg/mL. Based on the calculation results, a reasonable withdrawal period should not be less than 18 days at 25 °C for ENR in northern snakehead after treatment ceased.
研究领域:
水产养殖学
·
兽医学
·
药理学
✓ 已验证
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Kemeng Qin,Xinyu Sun,Ruican Wang,Jingmin Liu,Haitao Wang,Yi Wang,Shuo Wang
The development of sustainable and high-performance food packaging materials is crucial for addressing global food waste and environmental pollution issues. An innovative approach was proposed by using fish skin protein (FSP) from Channa argus as a natural carrier to promote the self-assembly of rosmarinic acid (RA) and curcumin (CUR) into stable nanospheres (FSP/CUR/RA) through non-covalent interactions such as hydrogen bonds, π-alkyl/π-anion interactions, and electrostatic interactions in this study. Compared with binary systems, the self-assembled FSP/CUR/RA ternary nanospheres exhibited significant advantages in colloidal stability and functionality: the optimized RA6 nanospheres have the smallest particle size (508 ± 19.30 nm), the highest ζ-potential (13.04 mV), and excellent antioxidant activity (ABTS + scavenging rate of 87.91 % at 20 μL), effectively overcoming the limitations of poor solubility and instability of CUR and RA. Upon incorporating the nanospheres into the active film, its overall performance was significantly enhanced. Specifically: Barrier properties were improved, with the water vapor transmission rate reduced to 4.06 × 10 −13 g m −1 ·Pa −1 ·s −1 , representing a 27.6 % decrease compared to the pure FSP film. Mechanical properties were optimized due to RA's effective inhibition of CUR aggregation, achieving a well-balanced tensile strength (TS) and elongation at break (EAB). The film demonstrated outstanding antioxidant capacity, with a free radical scavenging rate of 86.39 %, significantly higher than the control groups. Multifunctional characteristics were evident, including ultraviolet shielding capability, controlled release of CUR/RA, and complete biocompatibility (cell viability 100 %). In practical applications, FSP/CUR/RA films significantly extended the shelf life of fresh shrimp by reducing weight loss (3 % compared to 4.44 % in the control group), maintaining a low TVB-N level (11.84 mg/100 g after 3 days), and inhibiting lipid oxidation (TBARS < 1 mg MDA/100 g). This study not only provided a sustainable strategy for the value-added utilization of fish processing by-products but also promoted the design of multifunctional food packaging materials with enhanced preservation capability.
研究领域:
食品包装
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可持续材料
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生物高分子
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功能性薄膜
✓ 已验证
产品关联
DOI
高影响因子
Fishes
·
2024
·Vol. 9
·期 4
·120
·
IF 2.1
Xiaotian Zhang,Yuxia Wu,Yang Zhang,Jin Zhang,Kunci Chen,Haiyang Liu,Qing Luo,Shuzhan Fei,Jian Zhao,Mi Ou
Insulin-like growth factors (IGFs) play central roles in the growth and development of vertebrates. Blotched snakehead (Channa maculata), an economically significant fish, exhibits obvious sexual dimorphism and achieves sexual maturity in one year. However, the role of IGFs inC. maculataremains unknown. ThreeIGFgenes were identified inC. maculata, designated asCmIGF1-1,CmIGF1-2, andCmIGF2. The cDNA sequences of these genes are 1184, 655, and 695 bp, encoding putative proteins of 168, 131, and 215 amino acids, respectively, and all three proteins contain a conserved IGF domain. Quantitative real-time PCR (qPCR) revealed the predominant expression ofCmIGFsin the liver of adult fish, with higher expression levels observed in males. Notably,CmIGF1-1,CmIGF1-2, andCmIGF2displayed analogous expression profiles in the liver across various developmental stages, peaking at 365 days after hatching (dah). Subsequently, 600 individuals at 75 dah, at an early developmental stage, were randomly divided equally into six groups and reared in aerated 2 m × 2 m × 2 m cement ponds at 26.0 ± 1.0 °C. Following a one-week acclimatization period, fish without observed abnormalities were intraperitoneally injected with either 17α-ethynylestradiol (EE2) or 17α-methyltestosterone (MT) at a dose of 10 μg/g body weight. Three groups underwent short-term hormone treatment, and the remaining three groups underwent long-term hormone treatment, which included five injections at two-week intervals over ten weeks. The analysis ofCmIGFsexpression levels in the liver under different hormone treatments revealed that EE2suppressed the expression ofCmIGF1-1andCmIGF1-2while promotingCmIGF2expression. In females, MT up-regulated the expression ofCmIGF1-1andCmIGF2in a time-dependent manner, but consistently inhibitedCmIGF2expression. In males, MT promoted the expression ofCmIGFsin a time-dependent manner, reaching peak levels forCmIGF1-1,CmIGF1-2, andCmIGF2after 8, 10, and 2 weeks of injection, respectively. Additionally,CmIGF1andCmIGF2might exhibit a complementary relationship, with a compensatory increase inCmIGF2expression in response to lowCmIGF1concentration. These findings highlight the potential key role of IGFs upon growth and their regulation by sex steroid hormones inC. maculata, providing a crucial foundation for future research aimed at elucidating the molecular mechanisms underlying the growth dimorphism between female and male blotched snakeheads.
研究领域:
鱼类生理学
·
分子生物学
·
内分泌学
✓ 已验证
产品关联
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
·
2021
·
IF 2.231
Mi Ou,Kunci Chen,Dandan Gao,Yanduo Wu,Qing Luo,Haiyang Liu,Jian Zhao
Dmrt1 is an important transcriptional regulator that plays critical role in male gonadogenesis, testicular differentiation and development. In this study, Dmrt1 was cloned from blotched snakehead ( Channa maculata ) , which is designated as CmDmrt1 . Cm Dmrt1 encoded a putative protein with 293 amino acids and presented an extremely conserved DM domain. It was nearly expressed in the gonads, and the expression was more than 15 times higher in the testis than in the ovary. 1851 bp promoter sequence of CmDmrt1 was characterized and the methylation levels of the CpG sites were analyzed to detect sex-related differences. A significant negative correlation between CmDmrt1 expression and CpG methylation level of its promoter was found in the testis and ovary. During gonadal development, CmDmrt1 transcription displayed strong male-biased expression patterns, increased with the maturation of testis and reached the peak at 195 days after hatching (dah), which indicates a significant role of Dmrt1 in spermatogenesis. Steroid treatment could influence CmDmrt1 expression, and long-term 17β-estradiol (E2) treatment could induce the male-to-female secondary sex reversal (SSR), which resulted in the differentiated testis transformed to ovary or ovotestis. Meanwhile, CmDmrt1 expression was down-regulated to fairly low level in the ovary of the SSR XY fish, which was similar to that in normal XX females ovary. Our research illustrates that Dmrt1 is linked to testis differentiation and spermatogenesis in blotched snakehead, providing information for functional studies on sex differentiation and gonadal development of C. maculata , and scientific basis for the production practice of all-male snakehead breeding.
研究领域:
分子生物学
·
发育生物学
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水产养殖
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遗传学
✓ 已验证
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2018
·
IF 3.185
Cheng Li,Lindan Sun,Hanzuo Lin,Zhendong Qin,Jiagang Tu,Jun Li,Keping Chen,Sarath Babu V,Li Lin
Autophagy is a degradation cellular process which also plays an important role in virus infection . Glutamine is an essential substrate for the synthesis of glutathione which is the most abundant thiol-containing compound within the cells and plays a key role in the antioxidant defense and intracellular signaling . There is an endogenous cellular glutathione pool which consists of two forms of glutathione, i.e. the reduced form (GSH) and the oxidized form (GSSG). GSH serves as an intracellular antioxidant to maintain cellular redox homeostasis by scavenging free radicals and other reactive oxygen species (ROS) which can lead to autophagy. Under physiological conditions, the concentration of GSSG is only about 1% of total glutathione, while stress condition can result in a transient increase of GSSG. In our previous report, we showed that the replication of snakehead fish vesiculovirus (SHVV) was significant inhibited in SSN-1 cells cultured in the glutamine-starvation medium, however the underlying mechanism remains enigmatic. Here, we revealed that the addition of L-Buthionine-sulfoximine (BSO), a specific inhibitor of the GSH synthesis, could decrease the γ- glutamate-cysteine ligase (GCL) activity and GSH levels, resulting in autophagy and significantly inhibition of the replication of SHVV in SSN-1 cells cultured in the complete medium. On the other hand, the replication of SHVV was rescued and the autophagy was inhibited in the SSN-1 cells cultured in the glutamine-starvation medium supplemented with additional GSH. Furthermore, the inhibition of the synthesis of GSH had not significantly affected the generation of reactive oxygen species (ROS). However, it significantly decreased level of GSH and enhanced the level of GSSG, resulting in the decrease of the value of GSH/GSSG, indicating that it promoted the cellular oxidative stress . Overall, the present study demonstrated that glutamine starvation impaired the replication of SHVV in SSN-1 cells via inducing autophagy associated with the disturbance of the endogenous glutathione pool.
研究领域:
病毒学
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细胞生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）