International Journal of Analytical Chemistry
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2018
·
IF 1.479
Yang Tian,Yue Wang,Shanshan Wu,Zhian Sun,Bolin Gong
Surface-imprinted polymers (MIPs) microspheres with the ability to specifically recognize water-soluble molecules were prepared using self-made monodisperse porous poly(chloromethylstyrene-co-divinylbenzene) beads as the solid-phase matrix and ampicillin (AMP) as the template molecule. MIPs were synthesized using different template molecule: monomer: crosslinker ratios and the optimum preparation ratio were obtained by measuring adsorption. The maximum equilibrium amount of adsorption by the MIPs reached 115.62 mg/g. Scatchard analysis indicated that the MIPs contained two types of recognition sites: specific and nonspecific. Based on the adsorption kinetics, adsorption equilibrium was reached after 30 minutes. Penicillin G, amoxicillin, and sulbactam acid were used as competitive molecules to research the selective adsorption capacity of the MIPs. The imprinted material was found to have good selectivity with selectivity coefficients for penicillin G, amoxicillin, and sulbactam acid of 5.74, 6.83, and 7.25, respectively. The MIPs were used as solid-phase extraction filler, resulting in successful enrichment and separation of ampicillin residue from egg samples. Standard addition recovery experiments revealed that recovery was good with recoveries from the spiked samples ranging from 91.5 to 94.9% and relative standard deviations from 3.6 to 4.2%. The solid-phase extraction MIPs microcolumn was reused 10 times, where it maintained a recovery rate of over 80%. This work presents a sensitive, fast, and convenient method for the determination of trace ampicillin in food samples.
研究领域:
分析化学
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食品科学
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生物医学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 11
·3287-3296
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IF 3.969
Deli Xiao,Pierre Dramou,Nanqian Xiong,Hua He,Danhua Yuan,Hao Dai,Hui Li,Xiaomei He,Jun Peng,Nan Li
Fluoroquinolones (FQs) have emerged as one of the most important class of antibiotics. Due to their low concentration in bio-matrix samples which contain a lot of interfering substances, the efficient solid phase extraction and accurate determination of FQs remain a challenge. In this paper, a new strategy for the isolation and enrichment of FQs from egg samples was obtained by molecularly imprinted polymers on the surface of magnetic carbon nanotubes (MCNTs@MIP), which not only can be collected and separated rapidly by an external magnetic field, but also have a high specific surface area, outstanding mechanical properties and specific recognition for FQs. MCNTs@MIP were prepared using ofloxacin as a pseudo template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker. The characteristics of the MCNTs@MIP were assessed by transmission electron microscopy (TEM), multipoint Brunauer–Emmett–Teller (BET) analysis, vibrating sample magnetometry (VSM), X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy. The results of the adsorption experiments not only demonstrated rapid dynamic adsorption but also showed a high selectivity toward FQs. An extraction method using MCNTs@MIP coupled with high performance liquid chromatography (HPLC) was developed for the determination of four FQs in egg samples. The recovery of four FQs ranged from 95.2% ± 3.2% to 100.7% ± 3.1% and the detection limits ranged from 0.25–0.40 ng g−1. The results demonstrate that the proposed method based on pseudo template MCNTs@MIP is a promising approach for the preconcentration, purification, and simultaneous analysis of four FQs in bio-matrix samples.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
Analytical Methods
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2012
·Vol. 4
·期 4
·1005-1011
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IF 1.547
Jinhuan Kong,Yuzhi Wang,Chan Nie,Dan Ran,Xiaoping Jia
Novel magnetic molecularly imprinted polymers were prepared using bilayer modified Fe3O4 magnetite as the magnetically susceptible component, oxytetracycline and chlortetracycline as the mixed-template molecules, and methacrylic acid as the functional monomer. The magnetic polymers were applied to the separation of tetracycline antibiotics from egg and honey samples. The sample, polymers and extraction solvent were blended and stirred together. The extraction and clean-up procedures were carried out in a single step. The analytes could be extracted from the sample matrix to the polymers directly or through the extraction solvent as the medium. When the extraction was complete, the polymers which had adsorbed the analytes were easily separated from the sample matrix by an adscititious magnet. The analytes eluted from the polymers were determined by high liquid chromatography spectrometry. The recoveries obtained to be 76.2% to 95.8% with relative standard deviations in the range of 3.1–13.2%. The limit of detection was less than 0.2 ng g−1. The feasibility of this method was proven by analysis of incurred egg and honey samples. Compared with the classical method which uses more steps including solvent extraction, centrifugation, clean-up and concentration by solid-phase extraction, the proposed method decreased the complexity and improved the reliability.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2017
·
IF 7.504
Yan-Fei Wang,Yang-Guang Wang,Xiao-Kun Ouyang,Li-Ye Yang
We herein describe a novel adsorbent based on molecularly imprinted polymers (MIPs) on the surface of magnetic carboxylated cellulose nanocrystals (Fe3O4@CCNs@MIPs) for the separation and purification of six fluoroquinolones (FQs) from egg samples. The obtained Fe3O4@CCNs@MIPs not only exhibited a large surface area and specific recognition toward FQs, but also were easily gathered and separated from the egg samples using an external magnetic field. The morphologies and surface groups of the Fe3O4@CCNs@MIPs were assessed by X-ray photoelectron spectroscopy, transmission electron microscopy, vibrating sample magnetometry, X-ray diffraction, thermogravimetric analysis, Fourier transform infrared spectroscopy, and Brunauer–Emmett–Teller surface area analysis. The Fe3O4@CCNs@MIPs exhibited high selectivity toward six structurally similar FQs. An enrichment approach was established for the measurement of six FQs from egg samples using Fe3O4@CCNs@MIPs coupled to high-performance liquid chromatography. The recovery of spiked FQs ranged from 75.2–104.9% and limit of detection was in the range of 3.6–18.4 ng g–1 for the six FQs. Therefore, the proposed method is a promising technique for the enrichment, separation, and determination of FQs from biomatrices.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 12
·8303-8312
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IF 3.9
Na Li,Mengnan Liang,Hao Zhang,Zhongxia Hua,Ling Ma,Yanyu Qi,Ke Wang
The excessive use of quinolones (QNs) has seriously threatened human health. In this study, a novel functionalized magnetic covalent organic framework Fe3O4@SiO2@Ah-COF was fabricated with biphenyl-3,3′,5,5′-tetracarbaldehyde and hydrazine hydrate (85%) as monomers and was used as a magnetic solid-phase extraction (MSPE) absorbent for the determination of 24 QNs in water and egg samples through ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The extraction parameters of MSPE were optimized, including pH, adsorbent dosage, adsorption time, and eluent type. An effective and rapid detection method was then established, which showed good linearity (R2 ≥ 0.9990), low limits of detection (0.003–0.036 μg L−1) and low limits of quantitation (0.008–0.110 μg L−1) for QNs. The good recoveries of 24 QNs in water and egg samples were in the range of 70.3–106.1% and 70.4–119.7%, respectively, with relative standard deviations lower than 10% (n = 5). As a result, Fe3O4@SiO2@Ah-COF is a promising magnetic adsorbent, and the established method was successfully applied for the determination of 24 QNs in water and egg samples.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2022
·Vol. 38
·期 6
·1453-1460
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IF 2.726
Yi Jiaqi,Li Xiaoshuang,Cui Di,Han Lixia,Jiang Wei,Zhang Renguo,Niu Na,Chen Ligang
A novel fluorescent probe has been constructed based on fluorescence resonance energy transfer(FRET) between upconversion nanomaterials(UCNPs) NaYF 4 :Yb,Er and gold nanoparticles(AuNPs). The fluorescent “off-on” switching was formed for the detection of thiamphenicol(TAP) in egg samples. The fluorescence of UCNPs can be quenched to a certain degree by AuNPs. After adding TAP, the AuNPs generated aggregation and the fluorescence of UCNPs was recovered. The synthesized amination UCNPs and AuNPs were characterized by Fourier transform infrared spectroscopy(FTIR), UV-Vis, X-ray diffraction(XRD), energy dispersive spectrometer(EDS), and transmission electron microscope(TEM) techniques for observation and confirmation. As a model target, the detection of TAP has two linear ranges in the buffer solution within 0.01–0.1 µmol/L and 0.1–1 µmol/L using this fluorescent probe. The detection limit was obtained to be 0.003 µmol/L( S/N =3), which is favorable for trace analysis. The recovery of TAP from 98.2% to 105.3% was obtained, and the relative standard deviation(RSD) was from 2.5% to 4.3%. Furthermore, the method established in this study based on the UCNPs auto-low background fluorescence has high selectivity and strong ability to eliminate interference, which is beneficial to analyzing complex samples.
研究领域:
分析化学
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纳米技术
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材料科学
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光谱学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）