Animals
·
2020
·Vol. 11
·期 1
·36
·
IF 2.323
Fen-Fen Liu,Md. Abul Kalam Azad,Zhi-He Li,Jing Li,Kai-Bin Mo,Heng-Jia Ni
Simple SummaryHigh levels of inorganic Zn were supplemented to meet the nutritional requirements of Zn in piglets, which results in the development of intestinal microbial resistance and environmental pollution. Previous research indicated that organic Zn sources had higher bioavailability than inorganic Zn. There is limited information on the comparison of dietary Zn source dissolved in water with other Zn supplementation forms in piglets. Therefore, the present study was designed to compare the effects of three Zn supplementation forms (Zn-Met in the feed, ZnSO4in the feed, and ZnSO4dissolved in drinking water) on growth performance, Zn accumulation, activities of Zn-containing enzymes, expression of MT, and Zn transporters in piglets. In conclusion, Zn-Met and ZnSO4dissolved in drinking water had higher bioavailability in piglets.AbstractThe study aimed at determining the effect of different zinc (Zn) supplementation forms on Zn accumulation, activities of Zn-containing enzymes, gene expression of metallothionein (MT), and Zn transporters in piglets. Eighteen piglets were randomly divided into three groups: (a) a basal diet supplemented with 150 mg/kg Zn from Zn methionine (Zn-Met) in the feed (Zn-Met group), (b) a basal diet supplemented with 150 mg/kg Zn from Zn sulfate (ZnSO4) in the feed (ZnSO4,feedgroup), and (c) a basal diet supplemented with the same dose of Zn as in ZnSO4,feedgroup but in water (ZnSO4,watergroup). The results showed that Zn-Met added in feed and ZnSO4dissolved in drinking water significantly improved (p< 0.05) the Zn concentration in liver and jejunum and the apparent digestibility of Zn in comparison with the ZnSO4added in feed. In addition, dietary Zn supplementation as Zn-Met significantly increased (p< 0.05) the activity of alkaline phosphatase (AKP) in the jejunum of piglets in comparison with the ZnSO4,feedgroup. Furthermore, the Zn-Met and ZnSO4,watergroups showed an improved total superoxide dismutase activity (T-SOD) in the ileum as compared to the ZnSO4,feedgroup. Meanwhile, the qPCR and western blot results showed that Zn-Met and ZnSO4dissolved in drinking water increased the expression of MT in the jejunum in comparison with the ZnSO4added in the piglets’ feed. However, different Zn supplementation forms had no effect on the mRNA expressions of Zip4 and ZnT1 transporters. In conclusion, Zn-Met added in feed and ZnSO4dissolved in drinking water had higher bioavailability in piglets.Keywords:zinc;zinc methionine;metallothionein;mineral concentration;pig
研究领域:
动物营养学
·
兽医学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2020
·
IF 2.659
Mingfa Sun,Hongchao Jiao,Xiaojuan Wang,Victoria A. Uyanga,Jingpeng Zhao,Hai Lin
Crystalline amino acids ( AAs ) exhibit high nutritional values when supplemented AA-deficient diets. However, the AAs in crystalline form in the diet are absorbed quickly than protein-bound AAs, which may take an effect on AA utilization efficiency. In this study, 2 experiments were conducted to investigate the effect of encapsulated lysine-HCl ( Ly s) and DL-methionine ( DL-Met ) on the growth performance of broiler chickens. In experiment 1, a total of 432 one-day-old male Arbor Acres broilers were subjected to 3 dietary treatments (27 pens; 16 birds per pen) for 42 d. The control group was basal diets supplemented with crystalline Lys and DL-Met, and treatment groups had basal diets supplemented with encapsulated Lys and DL-Met at the levels of 80% and 60% of control diets ( 80CLM , 60CLM ), respectively. The growth performance, intestinal development, and transcription of AA transporters were determined. In experiment 2, 24 broiler chickens were subjected to the same treatments as in experiment 1. The plasma concentrations of free AAs were measured 0, 2, 4, and 6 h after feeding. The results showed that 80CLM treatment had no significant influence on production performance, carcass characteristics, and plasma free AAs content during the experiment compared with the control group ( P > 0.05). In addition, the 80CLM group moderately enhanced gut morphology development and increased AAs' absorption capacity. However, broilers fed the 60CLM diet had lower production performance and breast muscle weight than the control group ( P < 0.05), but increased villi height and B 0 AT mRNA expression level ( P < 0.05). At h 4 after feeding, the 60CLM broilers exhibited higher concentration of Ala, Cys, and total dispensable AAs than the control group ( P < 0.05). In conclusion, the result suggests that the supplemental levels of crystalline Lys and DL-Met can be effectively saved approximately for 20% by using the encapsulated form in broilers, with improvements to AAs utilization efficiency, while posing no detrimental effects on production performance. Encapsulated Lys and DL-Met would have greater potential for application when replacing crystalline AAs in broiler chickens.
研究领域:
动物营养学
·
家禽科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Ruyu Gong,Chunyue Ai,Baojun Zhang,Xianglei Cheng
The accumulation of organic selenium (Se) in grains is helpful in understanding its bioaccessibility in various Se-enriched rice cultivars after soil selenite treatments. In the present study conducted with two rice cultivars, the organic Se as well as the glutelin-derived Se and the selenomethionine (SeMet) percentage in grains increased with soil Se levels. The available Se that was detected in the forms of SeMet (∼40%) and selenite (∼12%) accounted for more than 50% of the total Se in grains, and no significant differences were observed between the two rice cultivars ( P > 0.05). Simulated gastrointestinal digestion showed that the digested grain SeMet increased with the soil Se treatment levels, whereas there were minimal changes in the percentages of selenite in the grain. Furthermore, approximately half of the available SeMet was derived from the grain glutelin . These results suggest that the non-glutelin-derived Se in rice should be further studied.
研究领域:
植物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TISSUE & CELL
·
2025
·
IF 2.5
Houru Liu,Caiyun Zhang,Lei Zhang,Jie Shen,Kan He,Bei Huang
The high global mortality rate of lung cancer underscores the urgent need for novel therapeutic strategies. Selenium (Se), an essential trace element, exhibits tumor-suppressive properties across malignancies. This study systematically evaluated the antitumor efficacy of selenomethionine (SeMet) compared with that of selenocysteine (SeCys) in Lewis lung carcinoma (LLC) cells in vitro and in tumor-bearing mice in vivo . Compared with SeCys (IC 50 = 45.89 μM), SeMet demonstrated superior cytotoxicity against LLC cells (IC 50 = 30.19 μM) and significantly inhibited proliferation and migration by inducing apoptosis and ferroptosis. In vivo , SeMet treatment inhibited tumour growth by 50.87% through the suppression of angiogenesis, outperforming SeCys (27.3%). Transcriptomic analysis revealed the downregulation of proangiogenic chemokines ( Cxcl1 , Cxcl2 , Cxcl3 , Cxcl5 ) and the upregulation of antitumor chemokines ( Cxcl9 , Cxcl16 ) in SeMet-treated tumors. Additionally, SeMet enhanced the activity of antioxidant enzymes (T-SOD and GSH-PX) and reduced the levels of proinflammatory cytokines (IL-6 and TNF-α) without hepatorenal toxicity. These findings establish SeMet as a multifaceted therapeutic candidate for lung cancer through dual induction of apoptosis-ferroptosis and angiogenesis inhibition.
研究领域:
肿瘤学
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分子生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Xuejian Liu,Junjie Ren,Daoyi Chen,Zhenyuan Yin
The continuously increasing CO 2 emissions since industrialization has sparked widespread concerns on environmental and climate change issues. Carbon sequestration and storage technologies (e.g., hydrate-based CO 2 sequestration) present promising potential for the disposing of excessive CO 2 in geological media. High gas uptake capacity and rapid CO 2 hydrate formation kinetics are required for the application of such a technology. In this study, we compared the kinetic promotion effects of sodium dodecyl sulfate (SDS) and L -methionine ( L -Met) on CO 2 hydrate with the objective of identification of an effective and eco-friendly CO 2 hydrate kinetic promoter. The experimental results suggested that L -Met (0.1 wt%) promotes CO 2 hydrates formation significantly with a gas uptake in CO 2 hydrate five times more than SDS at the same concentration. The hydrate morphology observations indicated that the wall-climbing hydrates associated with the addition of L -Met induced by the capillary driving force offer a possible explanation for the difference in the observed kinetics. A novel calculation method was developed to quantify the partition of CO 2 in gas, liquid and hydrate phases. Increasing the initial gas–liquid ratio promotes CO 2 stored in the hydrate phase as solid rather than in the liquid phase as dissolution. We further demonstrated the reusability of L -Met in five consecutive cycles of CO 2 hydrate formation and dissociation. It was assessed that L -Met as a CO 2 hydrate kinetic promoter is superior to SDS and can be employed as an efficient, reliable and eco-friendly kinetic promoter for the hydrate-based CO 2 sequestration technology.
研究领域:
环境科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
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2024
·
IF 6.5
Zhenhua Zhang,Yangyang Cao,Jieru Wan,Yanli Wang,Rongrong Chen,Rumin Li,Dalei Song,Tao Zhang,Jingyuan Liu,Jun Wang
Nanocontainers loaded with corrosion inhibitors as fillers can endow the organic coating with self-healing abilities. However, the number of nanocontainers that can be added to the coating is limited, and the number of corrosion inhibitors that can be accommodated by the nanocontainers is also limited. These considerably mitigate the anti-corrosion properties of the self-healing coating. Herein, a self-healing double-layered composite coating (MK-VK/EP), l -methionine (L-met)-loaded dendritic silica (KCC-1) nanocontainers modified epoxy coating (EP) as the bottom layer (MK/EP) and vanillin (Van)-loaded KCC-1 modified EP as the top layer (VK/EP), was fabricated on the surface of carbon steel. The results of electrochemical impedance spectroscopy (EIS) show that the low frequency impedance modulus (|Z| 0.01Hz ) of MK-VK/EP is nearly two orders of magnitude higher than that of MK/EP or VK/EP in 3.5 wt% NaCl solution after 45 days. Furthermore, the self-healing rate (r self-healing ) of the scratched MK-VK/EP coating (S-MK-VK/EP) is still much higher than that of the scratched MK/EP or VK/EP in the last two stages of immersion. Meanwhile, there exists no apparent corrosion after 12 h of immersion from the optical image of S-MK-VK/EP. The results show that MK-VK/EP can provide better self-healing corrosion protection performance than the single coating (MK/EP or VK/EP). Hence, the synergistic effect of different corrosion inhibitors can greatly improve anti-corrosion abilities of the coating, which will provide an effective approach for corrosion protection of metal in marine environment.
研究领域:
材料科学
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腐蚀与防护
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR IMAGING AND BIOLOGY
·
2012
·Vol. 14
·期 6
·699-707
·
IF 3.844
Mahounga Didel M.,Shan Lingling,Jie Cao,Du Changli,Wan Shunan,Gu Yueqing
Purpose A novel near infrared fluorescent probe, l -methyl-methionine (Met)–ICG-Der-02, was synthesized and characterized for in vivo imaging of tumors and early diagnosis of cancers. Method Met was conjugated with ICG-Der-02 dye through the amide bond function by ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride/ N -hydroxysuccinimide catalysis chemistry. Met–ICG-Der-02 probe uptake was evaluated on PC3, MDA-MB-231, and human embryonic lung fibroblast cell lines. The dynamics of Met–ICG-Der-02 was investigated in athymic nude mice prior to evaluation of the probe targeting capability in prostate and breast cancer models. Results Met–ICG-Der-02 was successfully synthesized. Cell experiments demonstrated excellent cellular uptake of Met–ICG-Der-02 on cancer cell lines without cytotoxicity. Optical imaging showed a distinguishable fluorescence signal in the tumor area at 2 h while maximal tumor-to-normal tissue contrast ratio was at 12 h Met–ICG-Der-02 post-injection. Additionally, dynamic study of the probe indicated intestinal and liver–kidney clearance pathways. Conclusion Met–ICG-Der-02 probe is a promising optical imaging agent for tumor diagnosis, especially in their early stage.
研究领域:
生物医学工程
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肿瘤学
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分子影像学
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 20
·9595-9601
·
IF 3.925
Hongbo Wu,Yingjie Xu,Ruyan Xie,Mingyu Tang,Lihua Chen,Fengyu Qu,Changhong Guo,Fang Chai
Facile and accurate detection of dopamine (DA) in biological samples is crucial for diagnostic needs. This study reports a portable colorimetric fluorescent probe for the tracing of DA based on orange-red emitting Au nanoclusters (AuNCs). The AuNCs were reduced and stabilized with L-methionine by strictly controlling the reaction conditions. The resultant L-methionine capped AuNCs (M-AuNCs) exhibit bright and stable orange-red emission with a quantum yield of 31.08%. The excitation/emission wavelength is located at 420 nm/610 nm, and the large Stoke's shift of 190 nm endows the M-AuNCs with great potential as a late-model sensing probe for life studies and environmental science. The M-AuNCs exhibit unique specificity and excellent sensitivity for tracing DA by an “on–off” strategy with a lower limit of detection (LOD) of 3.96 nM (S/N = 3) within a linear range of 10–100 nM. Notably, the colorimetric detection results can be explored as a visual sensor for sensitive, reliable and real-time/on-site detection of DA with the assistance of a smartphone. The LOD can be determined as 81.94 nM within a wide linear range of 0.1–1.0 μM by a smartphone. Furthermore, the M-AuNCs as a late-model potable probe allow simple and rapid discrimination of DA in serum, and the cellular imaging of the M-AuNCs indicated that the detection of DA can also be achieved in living cells, demonstrating their great feasibility for the tracing of DA in real samples.
研究领域:
生物化学
·
分析化学
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纳米科学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
Journal of Fungi
·
2024
·Vol. 10
·期 2
·151
·
IF 4.2
Xianran Zhu,Shaoying Zhang,Youwei Yu,Shengwang Li,Chao Yang,Yuan Chang
Alternaria alternatais the main pathogenic fungus of postharvest black spots in fruits and vegetables. This study aimed to explore the antifungal activity of methionine onA. alternatain vitro and to reveal related antifungal mechanisms through a metabolomics analysis. The results showed that the inhibitory effects of L-methionine (Met) treatment on mycelium growth, spore germination, and the germ tube elongation ofA. alternatawere enhanced with an increase in the Met concentration, but the inhibitory effects decreased when the Met concentration was higher than 50 mmolL−1. The results of propidium iodide staining and scanning electron microscopy showed that the Met treatment damaged the plasma membrane integrity of theA. alternataspores and caused an irreversible deformation of mycelium. In addition, after the Met treatment, the leakage of electrolytes, nucleic acid, and proteins in theA. alternatacells was significantly higher than that in the control group, indicating that the Met treatment increased the permeability of the cell membranes. Eighty-one different metabolites, divided into seven categories, were identified through the metabolomics analysis, including forty-three downregulated metabolites and thirty-eight upregulated metabolites. Among them, these differential metabolites were mainly involved in amino acid synthesis and metabolism, the pentose phosphate pathway, and the TCA cycle. Therefore, the antifungal effect of the Met treatment onA. alternatawas mainly to damage the integrity of the cell membranes, make nucleic acid and protein contents leak, and affect the TCA cycle, carbohydrate metabolism, amino acid synthesis metabolism, and the metabolic pathways associated with cell membrane biosynthesis. Thus, the growth and development ofA. alternatawere inhibited. The research enriched the investigation of the effect of the antifungal mechanism of Met treatment onA. alternataand provided a theoretical basis for the application of Met to prevent and treat postharvest black spots in fruits and vegetables.
研究领域:
植物病理学
·
食品科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
COMPOSITES COMMUNICATIONS
·
2024
·
IF 6.5
Xiaohe Miao,Xiaoyuan Duan,Huatao Cheng,Haoyu Duan,Zhiyi Zhang,Yaqing Liu,Shuaishuai Cheng
The dispersibility of fillers and interfacial interaction are crucial for polymer nanocomposites. Developing a simple, efficient and green method to simultaneously reduce and functionalize graphene oxide (GO) for preparing graphene/rubber composites with excellent dispersibility and enhanced interfacial interactions remains one of the main trends in developing and expanding the application of graphene in the rubber industry. In this work, VOC (volatile organic compound) -free and environmentally friendly L-methionine (L-Met) was selected as the functional modifier of GO. Then, L-Met-modified GO (MGO) was blended with natural rubber (NR) in an aqueous phase to prepare MGO/NR composites. The amphiphilic property of L-Met not only reduced GO but also endowed it with less agglomeration and excellent water dispersibility, which was a prerequisite for achieving uniform dispersion of GO in the NR matrix. In addition, the amino acids on the surface of MGO enhanced the compatibility between GO and the protein-phospholipid layer on the outer layer of NR latex particles. Meanwhile, L-Met containing sulfur bonds promoted rubber vulcanization, resulting in the formation of a cross-linked network between the modified GO and NR molecular chains. Compared to unmodified GO/NR composites, MGO/NR composites exhibited excellent mechanical properties and low heat build-up. More importantly, the solid tire prepared by using L-Met as the interface modifier for GO filled rubber showed lower rolling resistance and temperature rise, and the energy saving efficiency was improved. This strategy is expected to provide a new insight into the green production of organically modified graphene and the preparation of low-energy-consumption green graphene tires.
研究领域:
材料科学
·
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Yan Wang,Fuming Xue,Shuai Yu,Yan Cheng,Min Yin,Shichao Du,Junbo Gong
Cellulose derivatives such as hydroxyethyl cellulose (HEC), methylcellulose (MC) and hydroxypropyl methycellulose (HPMC) were employed to modify the morphology and growth of DL-methionine crystals. The methodology involved a systematic study through batch cooling crystallization, slow solvent evaporation, seeded single crystal growth, solid-state characterization, and molecular simulation. The presence of the polymeric additive, at very low concentrations (1–100 ppm), has modified the crystal shape from elongated leaf-like crystals to hexagonal plates or blocks with smaller aspect ratio. All three polymers exhibited strong retardation on crystal growth along the b -axis (length) and c -axis (width) direction. The growth inhibition degree depends on the types and concentrations of the additives, which can be ranked as HPMC > MC > HEC at same concentration. To further explore the mechanism of polymeric additives on morphology modulation and growth inhibition, Hirshfeld surface analysis, molecular electrostatic potential surface calculations, and molecular dynamic simulations of additive-crystal surface system as well as aqueous solution-crystal surface system were carried out. The molecular modeling revealed surface adsorption of additives on crystal faces via electrostatic interactions, van der Waals interactions, and hydrogen bonds. Other factors like hydrophobic interactions and steric hindrance effect of polymers also have significant impact on crystal growth inhibition.
研究领域:
化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE
·
2021
·
IF 4.242
Jiamin Li,Wenxuan Xu,Wencong Lai,Adong Kong,Zhou Zhang,Yuning Pang,Zhen Wang,Jikang Shentu,Xiongfei Wu,Kangsen Mai,Qinghui Ai
A 10-week feeding trial was conducted to evaluate the effect of dietary methionine (MET) on the growth performance, lipid metabolism and liver antioxidant function in juvenile large yellow croaker ( Larimichthys crocea ) fed with high lipid diets (HLDs). Large yellow croaker with an initial average weight of 8.59 g, were fed with basic HLD (lipid level at 18%, HL), basic HLD supplemented with 0.6% MET (HL/L-MET) and basic HLD supplemented with 1.2% MET (HL/H-MET). Results indicated that dietary MET did not affect weight gain, specific growth rate and feed efficiency ratio ( P > 0.05). The MET supplementation in large yellow croaker also did not affect the content of total lipid in whole body (HL = 9.57, HL/L-MET = 8.92 and HL/H-MET = 8.71%), liver (7.34 , 16.68 and 14.37%) and muscle (10.87 , 12.11 and 9.94%) ( P > 0.05). However, MET supplementation reduced the size and number of liver lipid droplets as dietary MET increased. Meanwhile, supplementation of MET in HLDs reduced serum total cholesterol (2.65, 2.14 and 1.91 mmol/L), low-density lipoprotein-cholesterol (0.27, 0.21 and 0.16 mmol/L) and triglyceride (4.80, 2.43 and 1.96 mmol/L) ( P < 0.05), but increased serum high-density lipoprotein-cholesterol (0.68, 0.77 and 0.83 mmol/L) ( P < 0.05). Dietary MET upregulated significantly the mRNA expression of genes related to mitochondrial fatty acid β-oxidation (PPARα and CPT1) and ER stress (GRP78, ATF6α and CHOP) ( P < 0.05). Furthermore, feeding fish with MET diets reduced serum alanine transaminase and aspartate transaminase activities, serum and liver malondialdehyde content, and liver 8-hydroxydeoxyguanosine level. Interestingly, feeding fish with MET diets increased liver total antioxidant capacity activity ( P < 0.05). In conclusion, dietary MET improved lipid metabolism and increased antioxidant capacity in the liver, but did not affect growth performance in juvenile large yellow croaker fed with HLDs.
研究领域:
水产养殖
·
营养学
·
鱼类生理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）