TALANTA
·
2013
·
IF 3.498
Qun Zeng,Yuzhi Wang,Na Li,Xiu Huang,Xueqin Ding,Xiao Lin,Songyun Huang,Xiaojie Liu
Eight kinds of green ionic liquids were synthesized, and an ionic liquid aqueous two-phase system (ILATPS) based on 1,1,3,3-tetramethylguandine acrylate (TMGA) guanidine ionic liquid was first time studied for the extraction of proteins. Single factor experiments proved that the extraction efficiency of bovine serum albumin (BSA) was influenced by the mass of IL, K 2 HPO 4 and BSA, also related to the separation time and temperature. The optimum conditions were determined through orthogonal experiment by the five factors described above. The results showed that under the optimum conditions, the extraction efficiency could reach up to 99.6243%. The relative standard deviations (RSD) of extraction efficiencies in precision experiment, repeatability experiment and stability experiment were 0.8156% ( n =5), 1.6173% ( n =5) and 1.6292% ( n =5), respectively. UV–vis and FT-IR spectra confirmed that there were no chemical interactions between BSA and ionic liquid in the extraction process, and the conformation of the protein was not changed after extraction. The conductivity, DLS and TEM were combined to investigate the microstructure of the top phase and the possible mechanism for the extraction. The results showed that hydrophobic interaction, hydrogen bonding interaction and the salt out effect played important roles in the transferring process, and the aggregation and embrace phenomenon was the main driving force for the separation. All these results proved that guanidine ionic liquid-based ATPSs have the potential to offer new possibility in the extraction of proteins.
研究领域:
生物化学
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蛋白质工程
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 21
·6445-6453
·
IF 3.969
Xiao Lin,Yuzhi Wang,Qun Zeng,Xueqin Ding,Jing Chen
A satisfactory protocol of protein extraction and separation has been established based on the ionic liquid aqueous two-phase system (IL-ATPS) for the purification of bioactive substances. Compared with the effects of eight different ionic liquids, 1-octyl-3-methylimidazolium bromide ([omim][Br]) was selected as the suitable ionic liquid. Based on the single-factor experiment, an initial serial investigative test was used to identify the optimal conditions of the extraction. Owing to their different isoelectric points, bovine serum albumin (BSA), hemoglobin (Hb) and lysozyme (Lys) were used to determine the effect of pH value on the protein extraction. Trypsin (Try) was used to confirm the protein activity. The linearity for analyzing BSA, Hb, Try and Lys was in the concentration range of 0.05–1.00 mg ml−1, 0.025–1 mg ml−1, 0.01–1.00 mg ml−1 and 0.01–1.00 mg ml−1, respectively, with a correlation coefficient of between 0.9985 and 0.9999. Limits of detection (LODs) were 16.47–7.02 μg ml−1 and RSDs of inter-day stability were less than 2.9%. Repeatability and precision were respectively lower than 5.3% and 1.1%. Under the optimum conditions, the average recoveries of BSA, Hb, Try and Lys were 90.5%, 94.5%, 92.7% and 93.8% and the obtained RSDs were 1.19%, 1.23%, 1.34% and 1.04%, respectively. According to UV spectra, conductivity, dynamic light scattering (DLS), and transmission electron microscope (TEM) images, the cluster phenomenon originating from IL itself or combined with protein was evaluated. As the driving forces which are involved in the partitioning of protein between the IL-rich phase and the phosphate phase, the cluster phenomenon could, in principle, be applied to a variety of different samples and exhibited potential value.
研究领域:
生物化学
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分离科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Tian Wang,Dehai Yu,Jian Wang,Bo Yang,Lei Ma,Shuangjiao Xu
The growing demand for sustainable proteins has highlighted cottonseed meal (CSM) as a promising high-protein byproduct. Addressing the challenge of valorizing the annual global production of over 10 million tons of CSM in cottonseed processing, this study investigated the use of choline chloride-ethylene glycol deep eutectic solvent (DES-4) for efficient protein extraction from CSM while reducing toxic gossypol content. Through Box-Behnken response surface optimization, we established optimal conditions (56 °C/3 h/40% water) that achieved 86.85% protein yield while remarkably reducing bound gossypol content from 10.24 mg/g to 2.76 mg/g. Molecular dynamics simulations (MDS) revealed that DES-4 enhance protein recovery and gossypol separation through synergistic mechanisms involving stabilizing solubility, relaxing matrix structure, and disrupting complexes. The obtained protein (CSMP-E) demonstrated superior solubility, emulsifying properties, and antioxidant activity compared to alkali-extracted counterparts. Furthermore, hydrolyzed DES-extracted protein (CSMP-EH) exhibited enhanced DPP-IV inhibitory activity (IC 50 = 1.949 mg/mL), suggesting its potential for type 2 diabetes management.
研究领域:
食品科学
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生物化学
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可持续农业
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Man Zhao,Yiqin Xie,Hemei Chen,Chunhui Deng
Zeolitic imidazolate frameworks (ZIFs) are composed of tetrahedral transition metal ions and imidazolate type organic linkers. This kind of metal organic frameworks (MOFs) combines the unique properties of both zeolites and MOFs. Furthermore, ZIFs can be assembled under mild biocompatible conditions. These advantages make them be primarily studied for biochemical analysis. In this work, polydopamine-modified hydrophilic magnetic ZIFs were synthesized in a simple and mild way for the efficient extraction of low-abundance peptides. Thanks to the low-coordinated Zn 2+ ions, the Fe 3 O 4 @PDA@ZIF-8 composites showed strong affinity towards low-abundance peptides, especially those with histidine residues . After enrichment, the sequence coverage of BSA and HSA tryptic digest got a significant increase. The solid phase method based on the magnetic ZIFs exhibited size-exclusion effect towards large-sized proteins, and proved to be effective even under the 200-fold dilution with BSA protein solution.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 12
·4067-4076
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IF 1.938
Jing Chen,Yuzhi Wang,Qun Zeng,Xueqin Ding,Yanhua Huang
A series of hydroxyl ammonium ionic liquids (ILs) have been designed and synthesized. An ionic liquid aqueous two-phase system based on N,N-dimethylethanolamine propionate ([DMEA][Pr]) ionic liquid was studied for the extraction of proteins. Based on the single-factor experiment, an initial serial investigative test was used to identify the optimal conditions. Five factors and four levels of orthogonal experiments were used to verify the optimum extraction conditions. The results show that under the optimum conditions, the extraction efficiency could reach up to 99.50%. The RSD of extraction efficiencies in precision experiment, repeatability experiment and stability experiment were 0.3% (n = 5), 1.1% (n = 5) and 1.5% (n = 5), respectively. The conformation of the proteins was not affected after extraction into the IL-rich phase in terms of the determination by UV-vis and FT-IR spectra. According to the determination of conductivity, dynamic light scattering, and transmission electron microscope images, the microstructure of the IL-rich phase and the possible mechanism for the extraction are investigated. Hydrogen bonding interaction, salt out effect and the aggregation phenomenon played important roles in the extraction. The circular dichroism spectral experiment analysis indicated that the secondary structures of the protein were unchanged after extract. Based on these findings, it is suggested that the method of hydroxyl ammonium-based ILs-ATPs have the potential to offer new possibility in the extraction of bio-analytes.
研究领域:
生物化学
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分析化学
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产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 20
·8358-8367
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IF 1.938
Jing Chen,Yuzhi Wang,Xueqin Ding,Yanhua Huang,Kaijia Xu
Hydroxy functional ionic liquids have been first designed and modified on the surface of silica-coated magnetic Fe3O4 nanoparticles (MNPs) for the investigation of the extraction performance of proteins by magnetic solid-phase extraction (MSPE) and characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). BSA was chosen as a model protein to investigate the effect of extraction parameters. Based on the single-factor experiment, an initial serial investigative test was used to identify the optimal conditions of the extraction, and the extraction efficiency could reach up to 86.92%. The RSD of extraction efficiencies in the precision, repeatability and stability experiments were 1.17% (n = 3), 3.79% (n = 3) and 4.82% (n = 3), respectively. Compared with the other four types of ionic liquids, hydroxy functional ionic liquids-modified magnetic nanoparticles have the highest extraction rates. The desorption of proteins was up to 94.91% when the concentrations of NaCl were greater than 1.1 mol L−1. Nearly 95% of MNPs could be recovered from each run, and extraction rates decreased significantly after five runs.
研究领域:
生物化学
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分析化学
✓ 已验证
DOI
高影响因子
Food and Bioprocess Technology
·
2022
·Vol. 15
·期 1
·190-202
·
IF 5.581
Wang Liling,Wang Yanbin,Qin Yuchuan,Liu Bentong,Zhou Yifeng
This study aimed to develop the extraction method of protein from edible oil for rapid detection. Firstly, aqueous biphasic systems (ABS) based on six hydrophilic ionic liquids (ILs) and three salts were developed and the phase diagram was drawn by turbidimetric point method. The binodal curves were fitted to the Merchuk equation. On this basis, the ABS composed of IL and salt were applied to extract protein from edible oil. The type of IL or salt, IL concentration, salt concentration, oil mass, extraction pH, and temperature on the extraction efficiency of protein from oil were investigated. The results showed that the optimum conditions for the extraction of protein from edible oil with ABS were as follows: 50% (w/v) K 3 PO 4 , 20% (w/v) [Bmim]Cl at 35 ℃, and pH 9.0. Under the optimal conditions, the protein extraction efficiency was almost 100%. Also, the extraction mechanism was studied and the main driving factors of protein extraction may be the hydrophobicity, electrostatic interaction, and salting-out between molecules. Finally, the method was used to detect the commercial edible oils from different sources. The results showed that the ABS could also be used to extract protein from other edible oils. In conclusion, the IL-based ABS method is simple and rapid for protein extraction from edible oil, and will highlight novel possibilities in the large-scale separation and purification of protein from oily solution.
研究领域:
食品科学
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化学工程
·
分析化学
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产品关联
DOI
高影响因子
Journal of Oleo Science
·
2024
·
IF 1.6
Chen Liu,Fu-sheng Chen
Effects of dry and wet grind on peanut oil and protein yield, oil bodies (OBs) stability, fatty acid composition, protein composition and functional characteristics were systematically analyzed. Results showed that peanut oil and protein yields reached highest at dry grind 90 s (92.56% and 83.05%, respectively), while peanut oil and protein yields were 94.58% and 85.36%, respectively, at wet grind 120 s. Peanut oil and protein yields by wet grind was 2.18% and 2.78% higher than that of dry grind, respectively. Surface protein concentration (Г) and absolute value of zeta potential of OBs extracted by wet grind (WOBs) were 11.53 mg/m 2 and 18.51 mV, respectively, which were higher than OBs extracted by dry grind (DOBs), indicating stability of WOBs was higher than DOBs. Relative contents of oleic acid and linoleic acid in peanut oil, essential and hydrophobic amino acids in protein extracted by wet grind were higher than dry grind. There was little difference in protein composition between wet and dry grind, but thermal denaturation degree of protein obtained by wet grind was lower than dry grind. Solubility, oil retention, emulsion stability, foaming and foam stability of protein obtained by wet grind were better than dry grind. Results from this study provided theoretical basis for grind pretreatment selection of aqueous enzymatic method.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Tao Wang,Xin Wu,Xuyuan Li,Wei Feng,Ren Wang,Kai Huang
Fungi proteins represent a potential alternative to animal proteins, which are regarded as a prospective source of edible proteins due to more sustainable and efficient cultivation of fungi. Nevertheless, the extraction of fungi proteins typically involves alkali extraction combined with acid precipitation (AEAP), yielding considerable amounts of salt that causes aggregation and inferior functional properties of proteins. In this study, we put forward a novel extraction method by direct filtration of Na + and OH − from the alkaline extraction solutions using ceramic membranes. This method enabled the continuous, efficient and cost-effective production of Pleurotus eryngii protein (PEP). The PEP prepared by this technology featured a uniform distribution of nanoparticles (∼129.5 nm) with higher water dispersity, colloidal stability and surface active properties as compared to that prepared by the AEAP method. Moreover, the microstructures of PEP can be tailored with various nanoparticle sizes and size distribution profiles, allowing for the adjustment of long-term stabilities and surface activities. Our study renovated the production technology of fungi proteins to enhance their functionalities as well as the application prospects in the future food industry.
研究领域:
食品科学
·
蛋白质工程
·
可持续农业
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mengge Xiao,Andrew Yuk Keung Leung,Zhonghao Zhu,Liang Zhu,Kaijun Xiao
In this paper, response surface methodology (RSM) was used to optimize the extraction process of proteins from duckweed and the protein extraction rate was increased to 24.11 ± 0.04 %. The duckweed protein extract (DP) was mixed with sodium carboxymethyl cellulose (CMC) and glycerol to prepare DP/CMC antioxidant membranes with different additions (0.5 % ∼ 2.5 %), and 2.0 % DP/CMC antioxidant membranes were used in blueberry preservation experiments. The results showed that the moisture permeability of DP/CMC antioxidant membrane was significantly reduced, the thermal stability was significantly improved, and the antioxidant property was significantly higher than that of CMC membrane ( p < 0.05). The lowest oxygen content in the 2.0 % DP/CMC membrane package compared to PE and CMC membranes may be due to the synergistic effect of aerobic respiration of blueberries and oxidation of antioxidants in the membrane, under which the loss of antioxidants in the blueberries was lowest and the quality of blueberries was effectively maintained. In this study, for the first time, antioxidant membranes with significant freshness preservation effects were prepared using duckweed protein extract (DP), demonstrating its great potential as an active packaging material for food preservation, and opening up a new development direction for the application of DP.
研究领域:
食品科学
·
生物技术
·
植物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Xueqin Ding,Yuzhi Wang,Ying Wang,Qi Pan,Jing Chen,Yanhua Huang,Kaijia Xu
A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been synthesized, and then magnetic chitosan graphene oxide (MCGO) composite has been prepared and coated with these functional guanidinium ionic liquids to extract protein by magnetic solid-phase extraction. MCGO-functional guanidinium ionic liquid has been characterized by vibrating sample magnetometer, field emission scanning electron microscopy, X-ray diffraction spectrometer and Fourier transform infrared spectrometer. After extraction, the concentrations of protein were determined by measuring the absorbance at 278 nm using an ultra violet visible spectrophotometer. The advantages of MCGO-functional guanidinium ionic liquid in protein extraction were compared with magnetic chitosan, graphene oxide, MCGO and MCGO-ordinary imidazolium ionic liquid. The proposed method has been applied to extract trypsin, lysozyme, ovalbumin and bovine serum albumin. A comprehensive study of the adsorption conditions such as the concentration of protein, the amount of MCGO-functional guanidinium ionic liquid, the pH, the temperature and the extraction time were also presented. Moreover, the MCGO-functional guanidinium ionic liquid can be easily regenerated, and the extraction capacity was about 94% of the initial one after being used three times.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Bingbing Xu,Zishan Hou,Zaixing Li,Wenjing Zhang,Xue Qin,Yi Xing,Chen Hong,Kai Wang,Nannan Wang
Antibiotic residue is a hazardous waste which contains much protein; the extraction of protein from antibiotic residue using neutral protease is an effective utilization path of antibiotic residue. In this work, an immobilized magnetic neutral protease (IMNP) was successfully prepared by immobilizing free neutral protease on the surface of magnetic carrier, and characterized by a series of analysis methods (SEM, BET, FTIR, TGA , XRD, and VSM). IMNP can retain > 90 % enzyme activity of free neutral protease and extract ∼36 % protein from streptomycin residue within 4 h at the optimal extraction condition (water content in antibiotic residue = 97.5 %, IMNP loading = 5 %, pH = 7.0, and extraction temperature = 50 ℃). The loss of neutral protease from the surface of IMNP and the possible poisoning of IMNP caused by the toxic substances in the streptomycin residue significantly affected the relative enzyme activity of IMNP (29.4 % enzyme activity of IMNP lost after 6th use). IMNP showed better storage performance, pH and thermal tolerance performance than those of free neutral protease.
研究领域:
生物技术
·
环境科学
·
酶工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）