Journal of Proteomics
·
2016
·
IF 3.867
Taotao Li,Qijie Jian,Yong Wang,Feng Chen,Chengwei Yang,Liang Gong,Xuewu Duan,Bao Yang,Yueming Jiang
Fusarium proliferatum as a filamentous fungal pathogen can produce mycotoxins that can contaminate postharvest fruits and thus impact risks on human health. The extracellular proteomes of F . proliferatum grown in the absence and presence of butylated hydroxyanisole (BHA) were analyzed comparatively. A total of 66 significantly different expressed secreted proteins were identified by LC-ESI-MS/MS analysis. The BHA treatment suppressed the accumulation of some pathogenic factors such as aspartic protease , cell wall degradation enzymes , porin , superoxide dismutase and glyceraldehyde-3-phosphate dehydrogenase. On the contrary, the BHA treatment increased the abundances of some proteins, such as ATP binding cassette transporter substrate-binding protein and lipopolysaccharide-assembly lipoprotein , involved in the growth of F . proliferatum . These findings suggest that BHA treatment could influence the pathogenic ability of F . proliferatum via inhibiting the levels of virulence factors and cell wall degradation-associated enzymes . Moreover, the induction of the growth-related proteins after the BHA treatment suggests that the livelihood of F . proliferatum might depend on the cost of reduced pathogenic ability. This study has provided some evidence for understanding the complicated mechanisms of F . proliferatum infection in an effort to develop new targets for the control of this fungal pathogen. Biological significance To better understand the inhibitory mechanism of F . proliferatum by butylated hydroxyanisole (BHA) treatment, a comprehensive proteomic analysis of the secreted proteins of F . proliferatum was firstly conducted. Among the 66 identified spots, 34 and 32 proteins were down- and up-accumulated significantly by BHA treatment, respectively. Many of the identified key protein species were involved in the pathogenic ability and the growth of F . proliferatum . This study is helpful for broadening our knowledge of the pathogenic mechanism of F . proliferatum .
研究领域:
微生物学
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植物病理学
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蛋白质组学
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分子植物病理学
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食品微生物安全
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真菌生物学
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DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2019
·
IF 7.015
Jian Zhou,Dan Sun,Lei Wang,Leiming Guo,Wei Chen,Fengjiao Yu,Yong Wang,Yang Yang
Mixed-matrix membranes blended with nanosized inorganic fillers have been widely used in ultrafiltration (UF) for large molecule separation. In this work, we provide a feasible route to construct mixed-matrix polysulfone (PSf) UF membranes via the nonsolvent-induced phase inversion process, utilizing sheet-like copper iodide superstructures (s-CuI) as fillers. The size of s-CuI in lateral dimension reaches micrometer scale, but the nanosheets can be well embedded in the polymer matrix with their 2D basal planes parallel to the membrane surface . On account of the stacking straight-through channel structure of s-CuI and its large dimension spanning across multiple membrane pores within the layer, the PSf membrane blended with 0.8 wt% content of s-CuI displays optimal UF performance with a pure water permeation (PWP) of 1473 L/(m 2 h bar) and a bovine serum albumin (BSA) rejection of 92%. In contrast, the pure PSf membrane only presents a PWP of 556 L/(m 2 h bar) and a BSA rejection of 58.2%. Besides, the enhancement of mechanical stability for the PSf/s-CuI 0.8% membrane is significant and the leakage of the s-CuI filler is negligible. Though the antifouling performance of the PSf/s-CuI 0.8% membrane against the protein BSA is limited, its flux is always 2–3 times higher than that of the pure PSf membrane in each step during a sequential test of fouling behavior . This study indicates that it is highly promising to employ designable superstructures as functional fillers to construct novel mixed-matrix membranes with good interfacial compatibility and superior performance.
研究领域:
材料科学
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膜分离技术
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高分子化学
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纳米复合材料
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生物分离工程
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DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Jia-Li Gu,Wen-Yan Pi,Wei-Feng Xie,Jia-Wen Gu,Ya-Jun Wang,Li Xiao,Yong-Jin Li,Jing-Fen Li
The present work is aimed to improve the antibody adsorption performance of phenyl-based mixed-mode adsorbents by adjusting the functional group of ligand. Two new mixed-mode adsorbents coupled with 4-(aminomethyl)phenol (AMP) and 4-(aminomethyl)benzonic acid (AMA) were designed based on the structure of benzylamine (BA), where a hydroxyl or carboxyl group was introduced. Bovine serum Immunoglobulin G (IgG) and bovine serum albumin (BSA) were used as the model proteins. The adsorption isotherms of IgG and BSA were investigated at different pH values ranging from 4.0–8.9 to evaluate the adsorption performance, and protein-ligand molecular interactions were explored through addition of sodium chloride and glycerol. The results showed that both of adsorbents coupled with AMP or AMA had lower saturated binding capacities ( Q m ) towards BSA and higher adsorption capacity ratio of IgG to BSA when compared to BA-based adsorbents. The contributions of electrostatic interactions and hydrophobic interactions to proteins binding were different among these phenyl-based mixed mode adsorbents. Finally, AMP-based adsorbent possessing high adsorption capacity ratio of IgG to BSA and salt tolerance was selected to separate IgG from mimetic serum with purity higher than 96 %. The results indicated adjusting the functional groups of ligand to regulate the electrostatic and hydrophobic interactions of protein-ligand was useful to improve the antibody separation performance of mixed-mode adsorbents.
研究领域:
生物分离工程
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免疫学
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生物材料
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蛋白质工程
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生物制药
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分子识别
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层析技术
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DOI
高影响因子
Plasma Processes and Polymers
·
2011
·Vol. 8
·期 10
·923-931
·
IF 1.643
Zhihong Zhang,Jun Dou,Fufeng Yan,Xianjun Zheng,Xili Li,Shaoming Fang
The present work describes the fabrication, characterization, and optimization of plasma polymerized pyrrole films, PPpy, prepared by continuous wave for application as the adhesion layers of bovine serum albumin (BSA). Plasma conditions used throughout the work produced significant differences in the chemical, physical, and electrochemical properties of the resultant PPpy films. Structural analysis and property characterizations of PPpy were carried out using Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and electrochemical workstation. Surface plasmon resonance spectroscopy was used to investigate the adsorption kinetics of BSA onto PPpy films. Moreover, when immersed in solutions or after BSA adsorption, the differences of electron transfer at the interfaces between the PPpy and the electrolyte solutions were determined by electro impedance spectroscopy. While there is a clear dependence of the protein adsorption affinity on the plasma polymerization condition employed during the film preparation. The relationship between film structure, electrochemical properties, polymer stability in aqueous buffer, and protein binding affinities are discussed.
研究领域:
生物材料
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表面科学
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生物医学工程
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分子生物学
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分析化学
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生物传感器
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DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 14
·5907-5916
·
IF 3.67
Qiu Yongting,Wang Zhihong,Liu Feng,Liu Junxia,Tan Kaiting,Ji Ruibo
Allelopathy by hydrophytes can be utilized to control algal blooms. This study was conducted to investigate the allelopathic effect (inhibition) of Scenedesmus quadricauda on Microcystis flos-aquae . When M. flos-aquae was co-cultured with S. quadricauda , the secretion of high-MW biopolymer by M. flos-aquae was inhibited by S. quadricauda . We further identified the allelochemicals and found that 4-tert-butylpyrocatechol (TBC) was the main active ingredient that could inhibit the growth of M. flos-aquae . When the dose of TBC was larger than 0.2 mg/L, almost all of the M. flos-aquae died. Additionally, TBC was found to suppress the growth of M. flos-aquae by disturbing the synthesis and secretion of proteins and polysaccharides and harming the chlorophyll to affect the light harvesting of algal cells. Therefore, TBC has the potential for use as a potential and promising algaecide to restrain the biomass of M. flos-aquae .
研究领域:
水生生物学
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环境微生物学
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化学生态学
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藻类生理学
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生物防治
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DOI
高影响因子
Small
·
2022
·Vol. 18
·期 29
·2202622
·
IF 15.153
Hao Zhu,Zixuan Chen,Yun Chen,Jun-Jie Zhu
Quantifying the binding kinetics and affinities of protein–small molecule interactions is critical for biomarker validation, drug discovery, and deep understanding of various biological processes at the molecular-scale. Novel approaches are demanded as most common label-free techniques are mass-sensitive, which are not suitable for the detection of small molecule interactions. Here, an optical imaging platform is developed to measure the binding kinetics of both protein–small molecules and protein–ions based on monolayer MoS 2 , an ultra-thin 2D material whose optical absorption is extremely sensitive to charge. A model is established to calibrate the optical response due to the charged analyte binding and it is applied to quantify the interactions between abl1 kinase and different small-molecule inhibitors. Such a presented method is capable of distinguishing different inhibitors binding to a wild or mutated kinase, which provides guidance for drug evaluation and drug mechanism exploration. The binding kinetics of calcium ions to calmodulin is also measured, further broadening the application field of the method. In addition, the imaging capability allows mapping the local binding kinetics of the molecular interactions with a high resolution, which reveals visible spatial variability and offers a promising tool for studying heterogeneous local interfacial interactions.
研究领域:
分子生物学
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药物发现
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生物传感器
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化学生物学
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生物物理
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转化医学
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纳米生物技术
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DOI
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2019
·Vol. 126
·期 1
·102-112
·
IF 2.683
Lin W.,Yuan D.,Deng Z.,Niu B.,Chen Q.
ObjectivesIn our previous research, we have developed a new combination disinfectant, glutaraldehyde‐didecyldimethylammonium bromide (GD). It was verified that GD had a strong effect on both Escherichia coli and Staphylococcus aureus. In this work, Candida albicans was selected as an object, and it could be killed by GD. We aimed to investigate the cellular and molecular mechanism of GD effecting on C. albicans.Methods and ResultsThe results of sterilization experiment indicated that GD was effective on C. albicans. Flow cytometry and atomic absorption spectrometry were applied to detect cell membrane damage of C. albicans. Luciferase reaction and Bradford method were carried out to detect ATP content and protein quantitation. Transmission electron microscopy was used for intracellular organelles morphological observation. In order to study changes in mitochondrial membrane potential, Rh 123 was used as an indicator. DNA conformation analysis was performed by molecular modelling and circular dichroism. The results indicated that membrane permeability was increased rapidly owing to GD effect, and the leaked K+ and Mg2+ were about 12·1 and 12·4 times those of the control, respectively, at 10 min after GD treatment. Simultaneously, ATP and protein also leaked rapidly out of the cell. Mitochondrial membrane potential was destroyed, succinic dehydrogenase activity was significantly decreased and DNA conformation was changed because of GD action.ConclusionsGlutaraldehyde‐didecyldimethylammonium bromide disinfected C. albicans through distorting cell membrane integrity and permeability, disturbing the intracellular homeostasis by intracellular substances leakage, especially K+, Mg2+, ATP and protein, causing electrolyte imbalance of mitochondria, changing DNA structure, which finally led to cell death.Significance and Impact of the StudyThis study focused on the cellular and molecular mechanism of GD as a disinfectant against C. albicans. It is important to provide theoretical support to GD against Candida albicans in practical application.
研究领域:
微生物学
·
分子生物学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2014
·
IF 2.694
Guihua Ruan,Meiping Wei,Zhengyi Chen,Rihui Su,Fuyou Du,Yanjie Zheng
A novel large-volume immobilized enzyme reactor (IMER) on small column was prepared with organic–inorganic hybrid silica particles and applied for fast (10 min) and oriented digestion of protein. At first, a thin enzyme support layer was formed in the bottom of the small column by polymerization with α-methacrylic acid and dimethacrylate. After that, amino SiO 2 particles was prepared by the sol–gel method with tetraethoxysilane and 3-aminopropyltriethoxysilane. Subsequently, the amino SiO 2 particles were activated by glutaraldehyde for covalent immobilization of trypsin. Digestive capability of large-volume IMER for proteins was investigated by using bovine serum albumin (BSA), cytochrome c (Cyt- c ) as model proteins. Results showed that although the sequence coverage of the BSA (20%) and Cyt- c (19%) was low, the large-volume IMER could produce peptides with stable specific sequence at 101–105, 156–160, 205–209, 212–218, 229–232, 257–263 and 473–451 of the amino sequence of BSA when digesting 1 mg/mL BSA. Eight of common peptides were observed during each of the ten runs of large-volume IMER. Besides, the IMER could be easily regenerated by reactivating with GA and cross-linking with trypsin after breaking the –C N– bond by 0.01 M HCl. The sequence coverage of BSA from regenerated IMER increased to 25% comparing the non-regenerated IMER (17%). 14 common peptides. accounting for 87.5% of first use of IMER, were produced both with IMER and regenerated IMER. When the IMER was applied for ginkgo albumin digestion, the sequence coverage of two main proteins of ginkgo, ginnacin and legumin, was 56% and 55%, respectively. (Reviewer 2) Above all, the fast and selective digestion property of the large-volume IMER indicated that the regenerative IMER could be tentatively used for the production of potential bioactive peptides and the study of oriented protein digestion.
研究领域:
生物化学
·
分子生物学
·
蛋白质组学
·
酶工程
·
生物材料
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2016
·
IF 2.76
Shanshan Zhang,Xuerui Chen,Shiping Ding,Qunfang Lei,Wenjun Fang
The interactions of human serum albumin (HSA) with four cationic gemini surfactants and four single-chain surfactants have been investigated from the measurements of fluorescence spectroscopy, dynamic lights scattering (DLS), zeta potential, and circular dichroism (CD). The fluorescence results show the binding strength of surfactants to HSA, along with the blue shift under the maximum emission wavelength ( λ max ) which is sensitive to the protein conformations. It has been found that the hydrodynamic diameters of the protein aggregates are enlarged and the electrokinetic potentials of them get positive with increasing the concentration of the surfactants. The conformational transformation of the secondary structures of surfactant–HSA aggregates has been confirmed through the quantitative analysis of the CD spectra. The isothermal titration microcalorimetry (ITC) has been employed to further analyze the binding process of single-chain surfactants to HSA. The comprehensive results obtained from various approaches have shown that both hydrophobic and electrostatic interactions are present in the cationic surfactant–HSA systems. Compared with the corresponding single-chain surfactants, the gemini ones have much stronger binding ability to induce the unfolding of HSA. These investigations on the interactions between HSA and surfactants with the altered chain architecture in different concentration regimes can facilitate the application of surfactant-protein systems in pharmaceutical, biotechnology and related fields.
研究领域:
生物物理化学
·
分子生物学
·
药剂学
·
蛋白质科学
·
药物代谢
·
胶体与界面科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）