INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION
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2026
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IF 1.9
Peng Wei,Junhui Li,Yuheng Chen,Jichao Zhang,Yuchen Liu,Yumin Xia,Yanping Wang
In this study, the hydrolytic degradation behavior of high-performance bio-based aromatic-aliphatic liquid crystal copolyesters (BTLCP)—synthesized from 4-hydroxybenzoic acid (HBA), phloretic acid (HPPA), vanillic acid (VA), and lactic acid (LA)—in proteinase K, phosphate buffer, and dilute alkaline solutions has been systematically and comparatively investigated. The degradation behavior was found to be correlated with the copolyester’s rigid and flexible molecular chain structure and composition. The weight loss after degradation was positively correlated with the LA content, as the aliphatic ester groups and amorphous regions of the molecular chain were most susceptible to chemical attack. The molecular chain further ruptured as degradation proceeded, causing the molecular weight to decrease sharply before leveling off after the seventh degradation period. Concurrently, the morphology of the copolyester flakes became coarser and more porous with increasing degradation time, suggesting a combined surface and bulk corrosion mechanism. Although the degraded copolyesters retained their liquid crystal behavior, the isotropic transition was observed below 350 °C, indicating a decrease in molecular chain rigidity. Moreover, the hydrophilicity of the copolyesters increased dramatically after degradation, especially for those containing more LA units, due to the generation of -OH and -COOH end groups from molecular chain scission. In summary, the results reveal that the hydrolytic degradation rate can be regulated by adjusting the composition and degradation medium. The synthesized BTLCPs containing more LA units exhibit excellent degradation performance and hydrophilicity, highlighting their potential for applications in biomedical and packaging fields.
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ACS Applied Materials & Interfaces
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2026
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IF 7.8
Xuan Ling,Ting Han,Pengfei Yang,Qian Zhang,Yong Xiang,Zhao Wang,Shikai Hu,Xiaoran Hu
Chronic obstructive pulmonary disease (COPD) is a respiratory disease imposing significant economic and health burdens to patients. The long latent period of COPD due to inhalation of PM 0.3 makes it difficult to prevent and identify the disease at an early stage. Herein, an environmentally friendly and long-term reliable nanofiber membrane with detection of COPD and filtration of PM 0.3 function is developed based on piezoelectric poly(l-lactic acid) (PLLA)/lactic acid-based copolyester piezoelectric elastomer (LCPE) composites. The LCPE is synthesized with low elastic modulus and high piezoelectricity, which firmly bonds with PLLA and acts as a stress concentration point in the fiber framework, converting local fiber vibrations into global film vibrations and helping to improve the acoustic-electric conversion of PLLA/LCPE nanofiber filtration membranes (PLNFM), which is 24 times that of neat PLLA under the same airflow. The PLNFM enables COPD identification by acoustic spectrum recognition through artificial intelligence (AI)-assisted algorithms, presenting a high recognition rate of over 92.3%. Meanwhile, the interwoven structure of PLNFM and the induced piezoelectric charge achieve PM 0.3 filtration efficiency as high as 99.975%. The respiratory disease identification function, high PM 0.3 filtration efficiency, and reusable performance of PLNFM provide great potential in intelligent wearable device fields.
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PLoS One
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2026
·Vol. 21
·期 2
·e0342746
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IF 2.8
Dandan Feng,Yakun Song,Zuqi Wu,Wuruo Liu,Yuting Pu,Yangcan Gao,Yuying Sun,Jiquan Zhang
Trypsin, a canonical serine protease in crustaceans, plays a crucial role in ontogeny and antibacterial defense. Whether these biological functions correlate with its catalytic characteristics remains unresolved in the freshwater shrimp Neocaridina denticulata sinensis . To address this gap, we characterized a trypsin gene from N. denticulata sinensis ( NdTryp ) and assessed both its biological roles and its prospective utility. NdTryp was predominantly expressed in the hepatopancreas, where it localized to storage cells (R-cells) and tubule-lining epithelial cells (ECTs). Across development, NdTryp transcripts were essentially absent during early embryogenesis but rose sharply at late stages, temporally coincident with hatching and the onset of feeding. After a challenge with Vibrio parahaemolyticus , the expression of NdTryp was induced, with the expression level significantly increased relative to the baseline expression level. RNA interference-mediated knockdown rendered shrimp more susceptible to infection and was accompanied by extensive hepatopancreatic injury, including epithelial detachment and disruption of the basement membrane. Biochemically, recombinant NdTryp (rNdTryp) displayed proteolytic activity over a broad temperature and pH span. Activity was differentially tuned by metal ions, with several divalent cations producing marked enhancement, whereas ferric iron exerted strong inhibition. Overall, our results showed that NdTryp functions as a multifunctional protease involved in both late embryonic development and innate antimicrobial defense. Furthermore, the robust stability of rNdTryp underscores its potential as an aquafeed additive and candidate for enzymatic biotransformation.
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POLYMER ENGINEERING AND SCIENCE
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2026
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IF 3.6
Lei Gong,Yuesheng Zhang,Enyang Xu,Hongtao Lu,Junrui Duan,Jianna Bao
In this work, poly(L-lactide-co-ε-caprolactone) (PLCL) copolymers with varying ε-caprolactone (ε-CL) contents that ranged from 0% to 10% were synthesized via ring-opening polymerization of L-lactide and ε-CL at 170°C for 1 h. Fully biodegradable PLCL/poly(D-lactide) (PDLA) blends with stereocomplex (SC) crystallites were fabricated through a solution blending approach. The effects of ε-CL content in PLCL on the crystallization behavior, thermal properties, mechanical performance, and degradation behavior of PLCL/PDLA blends were systematically investigated. The successful synthesis of PLCL was confirmed by 1 H NMR and FTIR analyses. The melting peaks observed in the high-temperature region and the characteristic diffractions in WAXD patterns provided evidence for the formation of SC crystallites in PLCL/PDLA blends. Compared with PLCL, the incorporation of PDLA significantly enhanced the crystallization rate, due to the nucleation function of SC crystallites. With increasing ε-CL content in the copolymer system, the T g and crystallinity of PLCL gradually reduced, and the melting peaks of HC and SC crystals shifted toward lower temperatures due to the reduced sequence regularity. A pronounced improvement in deformability was achieved by the presence of ε-CL segments. As the ε-CL content increased from 1.5% to 10%, the elongation at break rose from 12% to 192%, with the morphology of elongated fibrils. Higher ε-CL contents accelerated enzymatic degradation, with mass loss exceeding 75% after 7 days at 10% ε-CL, because the increased chain mobility may facilitate the penetration of water and Proteinase K into the blend, thereby promoting enzymatic erosion. Overall, high-performance PLA-based composites with excellent biodegradability were achieved via SC crystallization and modulation of ε-CL comonomer content. Summary PLCL copolymers with tunable ε-CL content were prepared and characterized. SC crystallization regulated the crystallization and thermal behavior. ε-CL segments enhance chain mobility and improve blend deformability. Enzymatic degradation kinetics were accelerated as ε-CL content increased.
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Biomaterials Advances
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2026
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IF 6.7
Zhenying Chen,Zhao An,Axiu Zheng,Yong Xu,Yingran Shen,Jun Lu
Microcurrent stimulation regulates ADSC chondrogenesis in a frequency-dependent manner; • 1 kHz MS enhances cartilage-like matrix deposition in ADSC-derived spheroids; • Optimal-frequency MS suppresses fibrocartilaginous, hypertrophic, and catabolic markers; • MS-induced chondrogenesis is associated with Ca 2+ –calcineurin–NFAT activation; • Frequency tuning provides a rational bioelectrical strategy for cartilage engineering. Adipose-derived stem cells (ADSCs) are promising for cartilage regeneration, but efficient, safe strategies to enhance chondrogenesis remain limited. This study examined frequency-dependent effects of microcurrent stimulation (MS) on rabbit ADSCs cultured as monolayers and three-dimensional spheroids. Cells were exposed to 0 Hz, 10 Hz, 100 Hz, 1 kHz, 10 kHz, or 60 kHz MS for 20 min daily. Chondrogenesis, extracellular matrix deposition, spheroid morphology, mechanical properties, inflammatory/catabolic responses, apoptosis, and Ca 2+ -calcineurin-NFAT signaling were evaluated. Among tested frequencies, 1 kHz MS most effectively enhanced chondrogenic differentiation, increasing SOX9, COL2A1, ACAN, and COMP expression and promoting collagen II and glycosaminoglycan deposition. It also improved spheroid morphology and mechanical properties while suppressing fibrocartilaginous, hypertrophic, inflammatory, and catabolic markers. MS did not alter medium pH, temperature, or reactive oxygen species levels and did not impair viability or increase apoptosis. Mechanistically, 1 kHz MS promoted Ca 2+ influx, NFAT nuclear translocation, and NFATc1 expression; inhibition of L-type Ca 2+ channels, intracellular Ca 2+ , or calcineurin attenuated this response. These findings identify frequency-tuned MS as a noninvasive strategy for promoting ADSC chondrogenesis and cartilage-like matrix formation. Download: Download high-res image (158KB) Download: Download full-size image
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TALANTA
·
2026
·
IF 6.7
Han Pang,Qiang Zhao
Rapid and sensitive detection of proteins is in great demand in disease diagnostics, drug discovery, basic research, and other biomedical applications. The emerging microscale thermophoresis (MST) technique, which enables to monitor fluorescence responses of fluorescent molecules under heating in real time, provides an attractive avenue for sensing transducer, with advantages in no separation and immobilization, ratiometric measurement, rapid analysis, and high throughput. Here, we developed a one-step, selective, and sensitive MST sensor for detection of protein based on a DNA switch assembled by binding a pair of aptamer probes on the same protein. These two aptamer probes share an additional short complementary sequence at terminals, and one is labeled with a fluorophore for signal output. They can bind to protein target, forming a sandwich complex and greatly enhancing local concentrations of aptamer probes, and the complementary sequences hybridize together, assembling a DNA switch with a duplex structure. The DNA switch can be opened upon heating in MST analysis, generating remarkable difference in MST responses over the unbound aptamer probes. Target protein can be detected by measuring the significant MST signal changes. We achieved detection of thrombin and platelet-derived growth factor BB (PDGF-BB) with this DNA switch-based MST sensor. The proposed strategy is promising for sensitive and selective detection of various target proteins.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 13.2
Lu Tian,Baiqiang Chen,Jingxue Huang,Yu Cao,Tingting Wu
Tumor-derived exosomal miRNAs serve as crucial biomarkers for clinical diagnosis and disease treatment monitoring. However, the need to lyse exosomes and extract miRNAs renders most methods expensive, laborious, and time-consuming. The simple, rapid, and sensitive analysis of exosomal miRNA still poses a significant challenge. Herein, we present a multifunctional biomimetic interface biosensor (MBIB) for exosome capture, miRNA release and amplified detection in one step. Catalytical hairpin assembly (CHA) probes functionalized MCF-7 cell membrane was firstly coated onto the glass substrate to fabricate the MBIB. Due to the homologous recognition ability, the MBIB could specifically capture MCF-7 cell-derived exosomes from samples and then release miRNA through membrane fusion. Finally, the released miRNA could be detected by the CHA probes on the cell membrane. Owing to the fluidity of the cell membrane, the CHA probes could collide effectively, resulting in an enhanced signal. The developed biosensor exhibited high sensitivity, reproducibility and stability. It has been successfully applied to the real samples and could accurately distinguish the tumor-bearing mice and healthy mice, showing great potential in clinical application.
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CHEMICAL ENGINEERING JOURNAL
·
2026
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IF 13.2
Wanning Gao,Shengyuan Shi,Youcong Li,Fangyuan Liu,Yudi Cheng,Jie Zheng,Meng Qiao,Shuai Yuan,Xing Zhang
The spatial organization of enzymes in natural cells enables efficient metabolic cascades through localized microenvironments and controlled intermediate diffusion. Mimicking this compartmentalized architecture in artificial systems remains a key challenge in biocatalysis. Here, we introduce a DNA-mediated self-assembly strategy to construct the biomimetic core-satellite structure of metal-organic frameworks (MOFs) that spatially organize multiple enzymes into defined compartments. In this system, DNA bridges larger “core” MOFs and smaller “satellite” MOFs, forming ordered assemblies with tunable microenvironments tailored for individual enzymes. Using glucose oxidase (GOx) and horseradish peroxidase (HRP) as a model cascade, we demonstrate that the core-satellite structure facilitates substrate diffusion, mitigates inhibition by intermediates, and enhances overall catalytic efficiency. This platform was further applied to support multi-enzyme cascades, including the two-enzyme system of N -acetyl hexosamine 1-kinase (NahK)/UDP- N -acetylgalactosamine pyrophosphorylase (GlmU) for glycosyl donor synthesis, and the three-enzyme system of β-galactosidase (β-Gal)/GOx/HRP for sequential cascade catalysis. Compared to the single MOF system, spatially compartmentalized MOFs exhibit up to 34-fold higher activity and improved thermal, solvent, and operational stability. This work provides a versatile platform for the design of cell-inspired biocatalytic systems and underscores the potential of DNA-guided MOF assembly in constructing programmable multi-compartment enzyme reactors.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Qingxu Song,Gongde Lan,Qian Li,Yang Lin,Xin Chen,Hong Liu,Yufeng Cheng,Yuxia Luan
Hypofractionated radiotherapy (HRT) is a promising approach for tumor treatment, owing to its straightforward elimination of tumor cells and stimulation of immunogenic cell death. However, clinical application of HRT is hindered by the inherent radio-resistance and inadequate immune activation. Herein, an injectable hydrogel platform was reported, enabling a cascade amplification of radiosensitization and immune activation to potentiate HRT. The hydrogel with encapsulated TLR7/8 agonist R848 is in situ constructed in tumor via L-norvaline-based polymer (LNP, a gelator). During HRT, the released L-norvaline from LNP suppresses L-arginine consumption through arginase 1 (ARG1) pathway, sparing adequate L-arginine that was used for radiosensitizer nitric oxide (NO) production by inducible NO synthase (iNOS). Meanwhile, R848 repolarizes tumor-associated macrophages (TAMs) from M2 type with rich ARG1 to M1 type with rich iNOS, reinforcing NO production and disrupting M2-type TAMs-induced radio-resistance and immunosuppression. Therefore, the hydrogel demonstrates a cascade regulation of radio-sensitization and immune activation, achieving powerful inhibition of tumor growth and tumor metastasis. This hydrogel provides a novel approach to potentiate HRT, holding great promise in clinical application.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Xiangpeng Han,Mengyu Zhang,Jiayi Peng,Jinsong Wu,Qingping Zhong
Vibrio parahaemolyticus and its biofilm threaten food safety and human health. Bacteriocins are known to inhibit various foodborne bacteria and their biofilms; however, few bacteriocins with antibiofilm activity against V. parahaemolyticus have been reported. Herein, a novel plantaricin Z057 from Lactiplantibacillus plantarum Z057 was extracted with ethyl acetate, purified by Sephadex LH-20 gel chromatography and reversed-phase high-performance liquid chromatography, and identified by nano-liquid chromatography coupled with tandem mass spectrometry. Its molecular mass and amino acid composition were 1272.61 Da and A-G-G-D-G-G-H-G-G-S-I-I-L-Y, respectively. Plantaricin Z057 presented a broad antibacterial spectrum against Gram-positive and Gram-negative pathogens. Moreover, it also exhibited excellent heat tolerance, maintained antibacterial activity at pH 2.0–4.0, and was susceptible to common proteases (pepsin, trypsin, papain, proteinase K). In addition, scanning electron microscopy revealed that plantaricin Z057 destroyed membrane integrity and caused cell deformation and cytoplasmic leakage. The crystal violet staining and XTT reduction assays revealed that plantaricin Z057 markedly decreased biofilm biomass and metabolic activity of V. parahemolyticus . Fluorescence microscopy showed that plantaricin Z057 efficiently inactivated sessile cells, and damaged integrated biofilm structure by reducing the extracellular polymer content. In summary, plantaricin Z057 exhibited significant potential for controlling V. parahaemolyticus and could be used in food production as a biological preservative.
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BIOMACROMOLECULES
·
2023
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IF 6.2
Lihuang Wu,Yuqi Wang,Xinyue Zhao,Hongli Mao,Zhongwei Gu
Poly(trimethylene carbonate) (PTMC), as one of the representatives of biodegradable aliphatic polycarbonates, has been found to degrade in vivo via surface erosion. This unique degradation behavior and the resulting nonacidic products make it more competitive with aliphatic polyesters (e.g., polylactide) in clinical practice. However, this surface degradation mechanism is complicated and not fully understood to date despite the findings that several reactive oxygen species and enzymes can specifically degrade PTMC in vitro. Herein, the biodegradation mechanism of PTMC was investigated by using possible degradation factors, distinct cell lines, and the inhibitors of these factors. The results demonstrate that PTMC undergoes a specific macrophage-mediated erosion. Macrophages tend to fuse into giant cells and elicit a typical inflammatory response by releasing proinflammatory cytokines. In addition, macrophages are suggested to primarily secrete enzymes (lipase specifically) to erode the PTMC bulk extracellularly as inhibiting their activity effectively prevented this eroding process. The clarification of the biodegradation mechanism in this work suggests that the degradation of PTMC highly depends on the foreign body response. Thus, it reminds the researchers to consider the effect of the microenvironment on the degradation and drug release of PTMC-based implantation devices and localized drug delivery systems.
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BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Tingting Wu,Xushun Liu,Hanjun Chen,Ying Liu,Yu Cao
Exosomal microRNAs (miRNAs) are emerging as attractive non-invasive and reliable biomarkers for disease diagnosis. In situ exosomal miRNA detection can avoid laborious and time-consuming exosome lysis, RNA extraction and effectively improve the accuracy. However, in situ exosomal miRNA detection is hampered by the low abundance of the targets and low permeability of the probes. Herein, an in situ exosomal miRNA sensing biochip based on multi-branched localized catalytic hairpin assembly (MLCHA) and photonic crystals (PCs) was proposed. The MLCHA probes could penetrate into the exosomes nondestructively due to its rigidity and generate amplified fluorescence signal upon recognizing the target miRNA. And then, the fluorescence signal was further enhanced by PCs to improve the sensitivity. The developed biosensor can not only detect exosomal miRNA in a concentration-dependent manner but also distinguish samples from cancer state and healthy state, which is potential for non-invasive clinical diagnostics.
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