JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2019
·Vol. 96
·期 12
·1367-1377
·
IF 1.72
Zhong-Yuan Liu,Da-Yong Zhou,Kanyasiri Rakariyatham,Hong-Kai Xie,De-Yang Li,Bei-Wei Zhu,Fereidoon Shahidi
The current study shows the compositional changes and oxidation development of clam ( Ruditapes philippinarum , R. philippinarum ) lipids and frying oils when subjected to different processing conditions. Parameters measured include acid value, peroxide value (POV), thiobarbituric acid-reactive substances (TBARS), total oxidation (TOTOX), lipid classes, fatty acid composition, phosphatidylcholine (PC), and phosphatidylethanolamine (PE) contents together with major glycerophospholipid (GP) molecular species. Deep-fat frying increased triacylglycerol (TAG) content and decreased the contents of PC, PE, and GP molecular species in clam in a time-dependent manner. Meanwhile, minor amounts of free fatty acids, diacylglycerols, monoacylglycerols, and polar lipids were detected in frying oils, indicating lipid migration between the clam and frying oils. The time-dependent increase of POV, TBARS, and TOTOX in fried clams and frying oils with concurrent reduction of docosahexenoic acid and eicosapentaenoic acid indicates extensive oxidative degradation of clam lipids. Moreover, the moisture-rich clam aggravated the deterioration of frying oils. Consequently, deep-fat frying significantly altered the lipid profile and decreased the nutritional value of clams.
研究领域:
食品科学
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营养学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Current Nanoscience
·
2024
·Vol. 21
·期 2
·345-353
·
IF 1.4
Liqing Zeng,Yazhen Chen,Baiqi Pan,Hui Yang,Yuyan Chen,Mei Xu,Songmei Chen,Fan Yang,Chongyu Gu
BackgroundMicroplastics is a new type of global pollutant that can absorb pollutants in the environment and enter the food chain. Arsenic (As) is a kind of heavy metal element, and its pollution to the environment has been triggered concern. Currently, the escalating threat to marine ecology posed by both microplastics and heavy metal pollution is garnering increasing attention, particularly concerning their detrimental impact on human health.MethodsThe aim of this paper is to study the adsorption of As by microplastics and their combined toxic effects on clams, which were determined by joint toxicity test.ResultsDuring the initial 48 hours, the fatality rates for larval clams exposed to As (III)-adsorbed microplastics, including polypropylene (PP), polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC), and polymethyl methacrylate (PMMA) at concentrations ranging from 10 mg/L to 500 mg/L, varied in the range of 0-30.0%, 0-10.0%, 0-30.0%, 0-15.0%, and 0-50.0%, respectively. Similarly, adult clams exhibited fatality rates within the ranges of 0-35.0%, 0-25.0%, 0-30.0%, 0-50.0%, and 0-15.0%. However, these rates increased significantly after 48 hours, reaching 80.0% (PP), 62.0% (PE), 40.0% (PS), 60.0% (PVC), and 70.0% (PMMA) for larval clams, and 85.0% (PP), 72.0% (PE), 40.0% (PS), 72.0% (PVC), and 65.0% (PMMA) for adult clams, respectively. In contrast, when exposed to microplastics concentrations exceeding 1000 mg/L with adsorbed As (III), both larval and adult clams experienced fatality rates that initially peaked between 55.0% and 100.0% within the first 48 hours. Throughout the entire incubation period with As (III) alone, the fatality rates for larval and adult clams remained relatively low, ranging from 0-20.0% and 0-15.0%, respectively.ConclusionThe mortality rate of clams directly correlated with the input of microplastic particles containing As (III); specifically, an increase in the concentration of microplastics resulted in higher fatality rates and accelerated death rates among the clams. Clams demonstrated varying toxicological responses to the different types of microplastics.
研究领域:
环境科学
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毒理学
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海洋生物学
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Shanqi Zhou,Mengru Fu,Kailun Luo,Zhihua Qiao,Cheng Peng,Wei Zhang,Juying Lei,Siyuan Ling,Bingsheng Zhou
Due to the characteristics of persistent organic pollutants (POPs), some legacy brominated flame retardants (LBFRs) were prohibited from use, and then gradually replaced by novel brominated flame retardants (NBFRs). However, till now little research focused on the effects of NBFRs on the benthos . In the present study, 0.5, 5, and 50 mg/kg dw of pentabromotoluene (PBT), hexabromobenzene (HBB), 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE), decabromodiphenyl ethane (DBDPE) and decabromodiphenyl ether (BDE209) were added into sediments to test freshwater clams ( Corbicula fluminea ). In the 35-day exposure experiment, C. fluminea had different enrichment behaviors in three treatment groups. It was conjectured that in the lower dose group, the clams ingested contaminants and tended to be stable over time. While in higher dose groups, the clams were induced by the chemicals, leading to the changes in physiological activities so that the concentrations showed a downward trend first and then went up. The half-lives of contaminants in freshwater clams were between 0.911 and 11.6 days. DBDPE showed stronger bioaccumulation ability than BDE209 in this study. Parabolic relationships were observed between log BSAF and log K ow values in clam tissues. Debromination, hydroxylation, and methoxylated products were detected. Additionally, the gill samples of C. fluminea exposed to 50 mg/kg dw of single substance were observed by scanning electron microscope (SEM), indicating that the adhesions, tissue hyperplasia, and messy cilia occurred on the surface. Our research potentially contributes to further evaluations of the environmental risks posed in sediments contaminated by PBT, HBB, BTBPE, DBDPE, and BDE209, particularly the benthic organisms.
研究领域:
环境科学
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生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2020
·Vol. 11
·期 4
·3483-3492
·
IF 4.171
Zhong-Yuan Liu,Yuan-Yuan Hu,Man-Tong Zhao,Hong-Kai Xie,Xiao-Pei Hu,Xiao-Chi Ma,Jiang-Hua Zhang,Yan-Hong Bai,Da-Yong Zhou
The formation and disappearance of aldehydes during simulated gastrointestinal digestion (SD) of fried clams was investigated in order to shed light on the underlying mechanism. Results from the thiobarbituric acid reactive substance (TBARS) and fluorometric assays using a specific aldehyde probe indicated that the SD (with lipase) of fried clams initially reduced (at the gastric stage), but subsequently increased (mainly at the intestinal stage) the contents of total aldehydes. Meanwhile, eight specific aldehydes including propanal, acrolein, trans-2-pentenal, hexanal, trans,trans-2,4-octadienal, trans,trans-2,4-decadienal, 4-hydroxy-hexenal and 4-hydroxy-nonenal in the digested meal were determined by using a high-performance liquid chromatography-tandem electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) method. Results indicated that the changes in the trend of the contents of the eight aforementioned aldehydes were similar to those of total aldehydes during SD (with lipase) of fried clams. However, a similar SD process without lipase time-dependently reduced the contents of total and individual aldehydes. Moreover, lipid classes and free fatty acids (FFAs) in the digested meal were determined to reveal the degree of hydrolysis of lipids during the SD process. Results indicated that the SD (with lipase) of fried clams significantly hydrolyzed triacylglycerols (TAG) and polar lipids (PL) and produced FFAs, but the SD process without lipase resulted in negligible lipid hydrolysis. Thus, our results demonstrated a positive correlation between lipid hydrolysis and aldehyde generation during the SD of fried clams. Alternatively, unsaturated FFAs instead of TAG and PL could have served as the main precursors for aldehyde generation due to their high oxidative susceptibility.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Chengwen Liu,Xulong Chen,Yan Ouyang,Liping Sun,Shaoyou Lu
A simple and sensitive method was developed and validated for the simultaneous determination of 21 amino acids in three aquatic products including grass carp ( Ctenopharyngodon idella ), clam ( paphia undulata ) and shrimp ( Metapenaeus ensis ) with high-performance liquid chromatography coupled with ionization tandem mass spectrometry (HPLC-MS/MS). Firstly, aquatic products samples were pretreated by enzymatic hydrolysis and the efficiencies of different hydrolysis conditions were investigated. Then both chromatographic and mass spectrometric conditions were optimized to improve sensitivity and accuracy for the analytes. Under the optimized conditions, the limits of detection (LODs) for 21 amino acids were in the range from 0.02 μg/g to 1.78 μg/g. The correlation coefficients and recoveries were ranged from 0.997 to 0.999 and 80–115 %, respectively, and the relative standard deviation was within the range of 0.6–8.7 %. The results showed that 21 amino acids were all detected in samples. Except for asparagine, glutamine, tryptophan, cystine, cysteine, aspartic acid and glutamic acid, there is no significant difference in the measurement of amino acids by HPLC-MS/MS and amino acid analyzer.
研究领域:
分析化学
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食品科学
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水产学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Zhong-Yuan Liu,Da-Yong Zhou,Zi-Xuan Wu,Fa-Wen Yin,Qi Zhao,Hong-Kai Xie,Jian-Run Zhang,Lei Qin,Fereidoon Shahidi
Phospholipid (PL)-enriched oils were recovered from six species of edible clams, namely Cyclina sinensis , Mactra chinensis Philippi , Mactra veneriformis Reeve , Meretrix meretrix , Ruditapes phliippinarum and Saxidomus purpurata, using a mixture of ethanol and hexane (1:1, v/v). The oils contained a high proportion of polyunsaturated fatty acids (PUFA) (26.78–45.36% of total FAs), especially eicosapentaenoic acid (EPA) (8.17–10.48% of total FAs) and docosahexaenoic acid (DHA) (7.83–21.34% of total FAs). The oils also contained a high percentage of PL (39.86–74.05% of total lipids). Among PL, phosphatidylcholine (37.40–52.19 mol%) and phosphatidylethanolamine (34.74–43.10 mol%) were dominant. At least 435, 442, 513, 438, 433 and 437 glycerophospholipid (GP) molecular species were characterized, respectively, in lipids from Cyclina sinensis , Mactra chinensis Philippi , Mactra veneriformis Reeve , Meretrix meretrix , Ruditapes phliippinarum and Saxidomus purpurata . Most of the predominant GP molecular species contained PUFA, mainly EPA and DHA, indicating that clam is a potential resource of PUFA enriched GP.
研究领域:
营养学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Kena Zhang,Quanzhi Xiao,Jianbo Jia,Yan Gao,Yanfei Zhou,Xiao-Xia Zhou,Bing Yan
The extensive use of cadmium sulfide nanoparticles (CdS-NPs), along with their natural formation through the complex biogeochemical transformation of anthropogenic cadmium ions (Cd2+), poses substantial risks to ecosystems and human health. Despite this, the mechanisms underlying the toxicity of CdS-NPs remain unclear. A key question is whether their toxicity arises from the nanoparticulate form of cadmium (Cd) or from the release of Cd2+. To explore this, we exposed freshwater clams (Corbicula fluminea) to environmentally relevant concentrations (0.01–1 mg/L) of CdS-NPs or Cd2+ for 10 days. Hematoxylin and eosin (HE) staining revealed significant damage to the digestive gland in both cases. Although CdS-NPs released some Cd2+ (≤10.4%), transcriptomic and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses indicated different toxicity mechanisms. CdS-NPs primarily induce ferroptosis, triggered by lysosomal dysfunction that releases Fe2+ into the cytoplasm, disrupting the cellular iron metabolism. In contrast, Cd2+ primarily induces an autophagic response, as evidenced by the upregulation of autophagy-related markers and activation of apoptosis pathways linked to mitochondrial membrane permeabilization. Overall, our findings suggest that the toxicity of CdS-NPs is not solely derived from Cd2+, highlighting the need to evaluate the risks posed by metal sulfide nanoparticles to benthic ecosystems.
研究领域:
环境毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Zhong-Yuan Liu,Da-Yong Zhou,Ao Li,Man-Tong Zhao,Yuan-Yuan Hu,De-Yang Li,Hong-Kai Xie,Qi Zhao,Xiao-Pei Hu,Jiang-Hua Zhang,Fereidoon Shahidi
This study aimed at investigating the formation and accumulation of 16 reactive aldehydes in clam ( Ruditapes philippinarum ) during oil frying in both the tissue and the oil using an HPLC-ESI-MS/MS methodology. After processing, the accumulation of acrolein, crotonaldehyde, pentanal, trans -2-hexenal, hexanal, trans , trans -2,4-heptadienal, heptanal, nonanal, trans , trans -2,4-decadienal and 4-hydroxy-2-nonenal was most noticeable in both fried clam and frying oil. Most of the aldehyde species showed a time- and temperature-dependent manner of formation and accumulation during frying due to continuous oxidative degradation under conditions employed. However, several species of aldehyde such as acrolein and trans -2-pentenal slightly decreased at higher temperatures and/or longer frying times, which may be due to the imbalance toward disappearance of aldehydes resulting from their evaporation under the extreme conditions. Presence of natural polyphenols in bamboo leaves significantly prevented the formation of aldehydes in both fried clam and frying oil due to their antioxidant activity ( P < 0.05).
研究领域:
食品科学
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分析化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2018
·Vol. 83
·期 12
·2976-2982
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IF 2.018
Zhong-Yuan Liu,Da-Yong Zhou,Xin Zhou,Fa-Wen Yin,Qi Zhao,Hong-Kai Xie,De-Yang Li,Bei-Wei Zhu,Tong Wang,Fereidoon Shahidi
Clam Ruditapes philippinarum was processed by hot-air drying and the changes of its lipids were evaluated by analyzing lipid classes, phospholipid classes, fatty acids, as well as oxidation parameters including peroxide value (POV), thiobarbituric acid-reactive substances (TBARS) value, total oxidation value (TOTOX), and oxidation test (OXITEST). The hot-air drying process reduced the contents of triacylglycerol, phosphatidylcholine, and phosphatidylethanolamine, indicating the hydrolysis of lipids. Meanwhile, the hot-air drying process significantly decreased the proportion of n-6 polyunsaturated fatty acid (PUFA), and consequently increased the PUFA ratio of n-3/n-6. Interestingly, the POV, TBARS and TOTOX decreased after the hot-air drying process. However, significant decline of the induction period for the dried clam tissue at elevated temperatures indicated their higher oxidation level, poor oxidative stability and reduction of shelf-life. Therefore, OXITEST method turned out to be an effective tool for estimating the level of lipid oxidation for hot-air dried clam.
研究领域:
食品科学
·
脂质化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Qingxia Yuan,Longyan Zhao,Qianqian Cha,Yi Sun,Hong Ye,Xiaoxiong Zeng
Sinonovacula constricta has been widely used as a health food and medicine in China, Japan, and Korea. In the present study, a water-soluble polysaccharide fraction (SCP-1) was prepared from S. constricta by enzyme-assisted extraction and purification of chromatography with DEAE-52 cellulose anion-exchange column and Sephadex G-100 size exclusion column. On the basis of the analytical results of high-performance liquid chromatography, Fourier transform-infrared spectroscopy, methylation analysis, and NMR spectroscopy, SCP-1 was found to have an average molecular weight of 15.63 kDa and a linear backbone of (1→4)-linked α-d-Glcp residue with one branch, α-d-Glcp, attached to the main chain by a (1→6) glycosidic bond at every five α-d-Glcp units. Furthermore, it was found that SCP-1 could significantly increase the viability of macrophages, enhance the capability of macrophage phagocytosis, increase the activity of acid phosphatase, and promote the production of nitric oxide, mouse tumor necrosis factor (TNF)-α, mouse interferon (IFN)-γ, and mouse interleukin (IL)-1β. The results suggest that SCP-1 possesses potent immunomodulating effect and may be explored as a potential biological response modifier.
研究领域:
海洋生物学
·
免疫学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2024
·
IF 4.1
Xiaoxue Li,Erzhou Yao,Jie Li,Weiqun Lu
Nano-TiO 2 is inevitably released into aquatic environment with increasing of nanotechnology industries. Study pointed that different individuality showed divergent behavioral and physiological response when facing environmental stress. However, the effects of nano-TiO 2 on tolerance of bivalves with different individualities remain unknown. In the study, clams were divided into two types of individuality - proactive and reactive by post-stress recovery method. It turned out that proactive individuals had quicker shell opening level, stronger burrowing behavior, faster feeding recovery, higher standard metabolic rate and more rapid ammonia excretion ability than reactive individuals after exposed to air. Then, the survival rate, hemocytes response and oxidase activity of classified clams were evaluated after nano-TiO 2 exposure. Results showed that after 30 d exposure, proactive individuals accelerated burrowing behavior with higher survival rate. Moreover, proactive clams had better adaptability and less hemocytes response and oxidative damage than reactive clams. The study highlights the individualities of marine shell fish determine individual capacity to adapt to environmental changes, play important roles in aquaculture and coastal ecosystem health.
研究领域:
海洋生物学
·
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zheng Zhou,Yu-Ying Zhang,Jing-Xuan Gao,Li-Xin Ma,Xu-Hui Huang,Jie Zheng,Xiu-Ping Dong,Lei Qin
This study investigated the changes in free amino acids (FAAs), 5′-nucleotides, and lipid compositions of Manila clams ( Ruditapes philippinarum ) collected from December 2017 to November 2018. High-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry was used to quantify twenty-one FAAs and six 5ʹ-nucleotides. Lipid profiles were explored by ultra-performance liquid chromatography coupled with a Q-Exactive Orbitrap mass spectrometer with an untargeted lipidomic approach. Results showed significant monthly changes of FAAs, 5′-nucleotides, and lipids profiles. Alanine , glutamic acid , glycine, and aspartic acid , which contributed to the sweet and umami flavor, were the most abundant FAAs in Manila clams. Totally 90 glycerides, 76 glycerophospholipids , 21 lyso-glycerophospholipids, and 52 fatty acids were identified in Manila clams. Among these lipids, most of them possessed a high-level of unsaturation degree. The unsaturation degree of lipids in February, March, and April was the highest, showing the best nutritional value of Manila clams.
研究领域:
海洋生物学
·
代谢组学
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食品科学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）