Foods
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2025
·Vol. 14
·期 15
·2711
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IF 5.1
Wanying Song,Xinyue Zhang,Huiyi Yang,Hanyu Liu,Baodong Wei
This study presents the development and optimization of a functional soy sauce fermented withCordyceps militaris(C. militaris), a medicinal fungus known for its high cordycepin and polysaccharide content. UsingC. militarisas the sole starter culture, the process aimed to improve both nutritional and functional properties. Response surface methodology was employed to optimize the entire fermentation process. During the koji stage, temperature, aeration, and inoculum concentration were adjusted to maximize protease activity and cordycepin production. In the fermentation stage, temperature, brine concentration, and water-to-material ratio were optimized to increase amino acid nitrogen and bioactive compound levels. Under optimal conditions (24 °C, 679.60 LPM aeration, 9.6% inoculum for koji; 32 °C, 12% brine, 1.53:1 water-to-material ratio for fermentation), the resulting soy sauce contained 1.14 ± 0.05 g/100 mL amino acid nitrogen and 16.88 ± 0.47 mg/100 mL cordycepin. Compared with traditionally fermented soy sauce, theC. militarisproduct exhibited a darker color, enhanced umami taste, and a distinct volatile profile featuring linoleic acid, methyl palmitate, and niacinamide. These results demonstrate the feasibility of usingC. militarisin soy sauce fermentation and its potential as a novel functional condiment with improved bioactivity and sensory quality.
研究领域:
食品科学
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微生物学
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生物技术
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营养学
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发酵工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2018
·
IF 4.019
Yong Tianqiao,Chen Shaodan,Xie Yizhen,Chen Diling,Su Jiyan,Shuai Ou,Jiao Chunwei,Zuo Dan
Recently, we've reported the anti-hyperuricemic effects of Cordyceps militaris. As a characteristic compound of C. militaris, we hypothesized that cordycepin may play a role in preventing hyperurecimia. Remarkably, cordycepin produced important anti-hyperuricemic actions, decreasing SUA (serum uric acid) to 216, 210, and 203 μmol/L (P < 0.01) at 15, 30, and 60 mg/kg in comparison of hyperuricemic control (337 μmol/L), closing to normal control (202 μmol/L). Elisa, RT-PCR and western blot analysis demonstrated that the actions may be attributed to its downregulation of uric acid transporter 1 (URAT1) in kidney. Serum creatinine levels and blood urine nitrogen and liver, kidney, and spleen coefficients demonstrated that cordycepin may not impact liver, renal, and spleen functions. In addition, we used computational molecular simulation to investigate the binding mechanism of cordycepin. Of which, van der Waals interaction dominated the binding. Residues TRP290, ARG17, ALA408, GLY411, and MET147 contributed mainly on nonpolar energy. This provided the theoretical guidance to rationally design and synthesis novel URAT1 inhibitors.
研究领域:
药理学
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生物化学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Cosmetic Dermatology
·
2025
·Vol. 24
·期 8
·e70343
·
IF 2.5
Wenlong Wang,Ming Zhong,Ronghua Liu,Shichun Zhao,Xianwen Wei,Qiwan Zheng,Qingyu Zhong,Yujie Shen,Feng He
Objective Although bioactive compounds from single herbs are extensively explored in cosmetics, the synergistic potential of herbal combinations remains understudied. This study aimed to evaluate the stability of a combined extract of Panax ginseng , Ganoderma lucidum , Cordyceps militaris , and several Asian plants (PGC), and its multifunctional efficacy for acne‐related skin dysfunction. Methods PGC was analyzed using high‐performance liquid chromatography (HPLC) for batch consistency and bioactive quantification. Network pharmacology and molecular docking were used to identify active components and targets and assess binding affinities, respectively. In vitro assays were conducted to evaluate antibacterial activity, skin barrier repair, keratinocyte migration, reactive oxygen species (ROS) reduction, anti‐inflammatory effects, and inhibition of lipid accumulation. The safety was tested via cytotoxicity assessments. Results HPLC analysis validated batch consistency and identified key bioactive constituents in PGC, including phenolic acids, flavonoids, and alkaloids. Integrated network pharmacology and molecular docking revealed multitarget mechanisms through the regulation of the IL‐17/TNF/NF‐κB axis pathway modulation. PGC exhibited potent antibacterial efficacy against acne‐associated pathogens ( Cutibacterium acne , MIC = 25 μg/mL), restored skin barrier integrity (filaggrin, +235%; loricrin, +261%), and sodium dodecyl sulfate (SDS)‐induced damage (85%). Concurrently, PGC accelerated keratinocyte migration (40%), reduced ROS (45%) and abnormal lipid droplet content (60%), and attenuated inflammatory responses (40% nitric oxide (NO) inhibition) while maintaining biosafety (no cytotoxicity ≤ 200 μg/mL). Conclusion PGC exemplified the translational potential of herbal compatibility, offering a multitargets solution for acne management through integrating antibacterial, barrier‐repair, anti‐inflammatory actions, and several other effects. This study established a network pharmacology‐guided framework for developing evidence‐based multitargets cosmetics.
研究领域:
化妆品科学
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植物化学
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皮肤科
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药理学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）