EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2015
·Vol. 242
·期 4
·537-546
·
IF 1.559
Yang Hongli,Huang Zhongping,Chen Yin,Zhang Changjun,Ye Mengdie,Wang Lili
Propolis, a well-known folk medicine in the world, has drawn growing attention due to the various bioactivities. An on-line method of coupling HPLC with 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) cationic radical scavenging detection (HPLC–ABTS) was developed to screen the single polyphenol possessing ABTS cationic radical scavenging activity in ethanol extracts of Chinese propolis (EECP). Ten common polyphenols were selected from the active fingerprints of ten EECP samples obtained from different provinces of China by the on-line HPLC–ABTS method and quantified by Trolox equivalent. Their contributions to the antioxidant property of EECP were compared. Furthermore, Pearson’s correlation analysis was applied to determine whether there was a correlation between the antioxidant activity of individual polyphenol and total antioxidant activity of EECP. The results indicated that the composition of polyphenols in ten EECP samples obtained from China was similar. But the different polyphenols contributed in different way. Caffeic acid, kaempferol, and galangin simultaneously were the most prominent contributors to the antioxidant properties of ten EECP. The contributions of pinobanksin 3-acetate, caffeic acid phenethyl ester (CAPE), and caffeic acid cinnamyl ester varied largely with the different geographical regions. They, except galangin, significantly correlated with the antioxidant property of propolis. However, pinobanksin, pinocembrin, chrysin, p -coumaric acid, and ferulic acid rarely contributed to the antioxidant property of Chinese propolis. Therefore, the on-line HPLC–ABTS method was convenient, effective, and comprehensive to evaluate the contributions of polyphenols to the antioxidant property for Chinese propolis.
研究领域:
植物化学
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药理学
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产品关联
DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Di Mu,Yu Jiang,Jing He,Yuanxing Zhang,Dahai Yang,Qin Liu,Zhuang Wang
Propolis is non-hazardous resinous substance mixture containing bioactive ingredients such as polyphenols, flavonoids and organic acid. It has been widely used as food supplement and immune adjuvant due to its benefits in anti-microbial and immunomodulation . Edwardsiella piscicida is a kind of threatening pathogen which could cause high mortality in turbot . However, whether propolis could enhance the innate immune response against E. piscicida infection in turbot remains unknown. In this study, we found dietary propolis addition could improve the expression of anti-oxidative stress related enzymes, e.g., SOD , CAT and GPT , and relieved the histopathological changes of juvenile turbot after E. piscicida infection. Moreover, propolis addition increased the expression of cytokines such as il-1β , il-6 and tnf-α in different organs of juvenile turbot. Importantly, rescued survival and decreased bacterial loads were observed in propolis feeding group. Taken together, these findings suggest that the important roles of propolis in protecting juvenile turbot from E. piscicida infection, indicating propolis might be applied as a promising immunopotentiator candidate in aquaculture.
研究领域:
水产养殖学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Jinman He,Gulden Goksen,Xinli Cong,Mohammad Rizwan Khan,Naushad Ahmad,Wanli Zhang
This study aims to develop zein co-encapsulated Wampee [ Clausena lansium (Lour.) Skeels] essential oil (WEO) and propolis ethanol extract (PEE) for incorporation into sodium alginate matrix (ZPWS). The films were evaluated for their morphological features, chemical structure, mechanical and barrier properties, as well as their resistance to high temperatures. Additionally, antibacterial effects and antioxidant activities of films were investigated. The functional properties of zein were also evaluated for its ability to effectively co-encapsulate WEO and PEE, achieving in delayed release. Overall, the ZPWS films exhibited excellent functional properties compared the original films. These enhancements included greater tensile strength, increased rigidity, heightened resistance to water, improved thermal stability, a more condensed structure, and the presence of strong antioxidant and long-lasting bacteriostatic properties. Finally, a real-time study on the edible coated films was carried out strawberries preservation. The ZPWS film demonstrated good potential for application in food preservation.
研究领域:
食品科学
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材料科学
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生物学
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产品关联
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 46
·5577-5588
·
IF 2.073
Qian Liu,Hai-Long Wu,Zhi Liu,Rong Xiao,Tong Wang,Yong Hu,Yu-Jie Ding,Ru-Qin Yu
A methodology for simultaneously measuring seventeen polyphenols in six Chinese raw propolis samples by using chemometrics-assisted high performance liquid chromatography-diode array detection (HPLC-DAD) was developed and validated. With the aid of a second-order calibration method based on an alternating trilinear decomposition (ATLD) algorithm, HPLC analysis was easily achieved within 16.5 min even in the presence of heavily overlapped peaks and unknown interferents. The average recoveries were in the range of 95.2% to 118.7% with standard deviation less than 7.0% for the selected raw propolis. Figures of merit including sensitivity (SEN), selectivity (SEL), root mean square errors of prediction (RMSEPs), etc. were calculated to validate the accuracy of the proposed method. For further confirmation, a multiple reaction monitoring (MRM) method based on LC-MS/MS was adopted. The paired F-test and t-test results showed that there was no significant difference between the MRM and the proposed method at the 95% confidence level, which indicated that the developed ATLD-assisted HPLC-DAD method is a suitable tool for the rapid analysis of Chinese raw propolis. Considering its advantages of simplicity, rapidity, precision and accuracy, it might also be extended as an alternative and available method for the quality monitoring of propolis raw material in the pharmaceutical industry.
研究领域:
分析化学
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药学
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产品关联
DOI
高影响因子
Food Biophysics
·
2022
·Vol. 17
·期 3
·386-396
·
IF 3.34
Wang Yuhang,Fu Yuying,McClements David Julian,Ba Chujie,Li Teng
Propolis is a natural bioactive compound that is being explored as a nutraceutical because of its potential health benefits. In this study, propolis-loaded biopolymer nanoparticles were fabricated from folic acid-modified carboxymethyl chitosan (FA-CMCS) using the pH-driven method. The mean diameter of the propolis-loaded FA-CMCS nanoparticles was about 180 nm, and transmission electron microscopy showed they had a spherical shape. Fourier transform infrared spectroscopy analysis demonstrated that hydrogen bonding, electrostatic, and hydrophobic interactions were the major driving forces responsible for nanoparticle formation. The nanoparticles had good colloidal stability at pH 2.0, 3.0, 7.0, and 8.0 but exhibited some aggregation at intermediate pH values. The nanoparticles were also stable to salt addition up to 400 mM NaCl. Propolis-loaded FA-CMCS nanoparticles exhibited improved antioxidant properties compared to pure propolis and propolis-free nanoparticles. A simulated digestion model (fed state conditions) indicated that different amounts of propolis were released in different gastrointestinal regions: around 25%, 15%, and 25% in the stomach, small intestine, and colon, respectively. Our results suggest that the nanoparticles fabricated in this study have potential as colloidal delivery systems for sustained release of propolis in the human gut.
研究领域:
药剂学
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生物材料
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营养学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
Fengjie Yu,Jianing Zhang,Yunmin Tao,Chunping Du,Wenchao Yang,Wenbin Chen,Xijuan Tu
In the present study, a high-throughput homogenous liquid-liquid extraction method was developed for fast sample preparation of multiple phenolic compounds in propolis. This method was proposed based on cooling samples array in subzero temperature to induce phase separation of ACN-H 2 O extractant. Due to the high-throughput ability, optimization of extraction parameters was rapidly achieved by using a 5 × 4 × 3 samples array. In addition, multiple arrays were investigated for evaluating the analytical performance of the high-throughput method, which indicated that limits of detection and quantification were ranged from 0.04 to 0.35 µg/mL and 0.12 to 1.05 µg/mL, respectively. Recoveries and precisions in inter-day high-throughput studies were in the range of 90.55–105.50% and 2.58–4.30%, respectively. Comparing with the conventional liquid extraction method, this ecofriendly high-throughput method presented remarkable advantages in reducing sample and chemical consumption, as well as saving labor and time cost. The proposed method might provide a valuable strategy for the design of high-throughput extraction procedures.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ANTIVIRAL RESEARCH
·
2024
·
IF 4.5
Zhanding Cui,Jing Zhang,Jinlong Wang,Jinlong Liu,Pu Sun,Jiaoyang Li,Guoxiu Li,Ying Sun,Juanbin Ying,Kun Li,Zhixun Zhao,Hong Yuan,Xingwen Bai,Xueqing Ma,Pinghua Li,Yuanfang Fu,Huifang Bao,Dong Li,Qiang Zhang,Zaixin Liu,Yimei Cao,Zengjun Lu
Porcine Reproductive and Respiratory Syndrome (PRRS) presents a formidable viral challenge in swine husbandry. Confronting the constraints of existing veterinary pharmaceuticals and vaccines, this investigation centers on Caffeic Acid Phenethyl Ester (CAPE) as a prospective clinical suppressant for the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). The study adopts an integrated methodology to evaluate CAPE's antiviral attributes. This encompasses a dual-phase analysis of CAPE's interaction with PRRSV, both in vitro and in vivo , and an examination of its influence on viral replication. Varied dosages of CAPE were subjected to empirical testing in animal models to quantify its efficacy in combating PRRSV infections . The findings reveal a pronounced antiviral potency, notably in prophylactic scenarios. As a predominant component of propolis, CAPE stands out as a promising candidate for clinical suppression, showing exceptional effectiveness in pre-exposure prophylaxis regimes. This highlights the potential of CAPE in spearheading cutting-edge strategies for the management of future PRRSV outbreaks.
研究领域:
兽医学
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病毒学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2011
·Vol. 16
·期 4
·3444-3455
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IF 1.988
Haisha Yang,Yuqiong Dong,Huijing Du,Haiming Shi,Yunhua Peng,Xiaobo Li
The antioxidant activities of the chloroform, ethyl acetate andn-butanol extract fractions from propolis collected in Anhui, China were evaluated in this study. The ethyl acetate fraction contained the highest amount of total phenolics and total flavonoids, and showed the greatest 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging capacities and Ferric Reducing/Antioxidant Power (FRAP). The antioxidant activity of twenty-two compounds isolated from the ethyl acetate fraction was also evaluated using the above-mentioned three assays. The results indicated that phenolics contributed to the antioxidant activity of propolis collected in Anhui, China. Therefore, propolis collected in Anhui, China and its phenolics might be used as a natural antioxidant.Keywords:propolis;antioxidant activity;phenolics;natural antioxidants
研究领域:
植物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Do Hee Kim,Eun Woo Jeong,Youjin Baek,Hyeon Gyu Lee
Propolis extract (PE) is a natural polyphenol with various biological activities; however, its application is limited due to low solubility and instability. To overcome these drawbacks, we encapsulated PE in nanoparticles (PE-loaded nanoparticles, PE-NP) using chitosan (CS) and hyaluronic acid (HA) as wall materials. We examined the physicochemical characteristics (size, polydispersity index, zeta potential, and entrapment efficiency) of PE-NP prepared with the various ratios of CS to HA and core to the wall material, and confirmed the optimal ratio for the formulation of PE-NP as 4:7 and 1:2, respectively. In addition, PE-NP significantly increased the solubility of PE by 2.43-fold compared to that of free PE ( p < 0.05). Moreover, the retention rate of total flavonoid content (TFC) in PE-NP was significantly higher than that in free PE after the heat treatment (90 °C) for 24 h ( p < 0.05). Furthermore, PE-NP showed significantly higher retention rates of TFC and DPPH radical scavenging effects than free PE after storage for 4 weeks at 30 and 50 °C ( p < 0.05). In conclusion, these findings suggest that PE-NP may be a promising approach to enhance the solubility and stability of PE and could provide helpful information for expanding the application of PE.
研究领域:
药剂学
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材料科学
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天然产物研究
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Qingzhi Ding,Yuan Zheng,Yongqi Zhu,Huamin Yang,Lin Luo,Haile Ma,Xiaoran Li
The individual and combined inhibitory activities of propolis extract (PE) and Hermetia illucens larval proteins (Black soldier fly larvae proteins) (BSFLP) against Aspergillus niger were investigated. The antifungal effects of PE, BSFLP and Synergy of PE and BSFLP (SPB) against Aspergillus niger were determined by mycelial growth , spore germination rate and hyphal elongation. Membrane integrity was measured by assessing changes in extracellular proteins, reducing sugars, and electrical conductivity . Intracellular MDA , H 2 O 2 , ergosterol , and mannan contents were also evaluated. SEM was used to observe the morphology of the Aspergillus niger mycelium . Additionally, AutoDock Tools software and multi-ligand docking methods were used to study the synergistic interactions of ligand combinations on the receptor. The results showed that the combination of PE and BSFLP had excellent effects. During the treatment of antifungal agents, the membrane permeability changed and a large amount of content leaked, while control check (CK) remained almost unchanged. Ergosterol content was found to increase, while mannan content decreased significantly, leading to cell death. In addition, SPB can cause abnormal accumulation of MDA and H 2 O 2 , leading to oxidative stress in cells. SEM results showed that morphology of Aspergillus niger treated with SPB was severe wrinkling, distortion and even breakage. Multi-ligand docking results showed that the binding affinity of receptor 1KS5 to 14 complex ligands increased, as indicated by the decrease in docking energy values. These results provide scientific basis for further study on the application of SPB and development of effective antifungal products.
研究领域:
微生物学
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食品科学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2012
·
IF 2.823
Haiming Shi,Haisha Yang,Xiaowei Zhang,Liangli (Lucy) Yu
Fifteen propolis samples collected from different regions of China were investigated and compared for their phytochemical composition and anti-inflammatory and radical scavenging properties. Eleven compounds including caffeic, p-coumaric, ferulic, isoferulic, and 3,4-dimethylcaffeic acids, pinobanksin, chrysin, pinocembrin, galangin, pinobanksin 3-acetate, and caffeic acid phenylethyl ester were quantified for the 15 propolis samples using a UHPLC method, whereas 38 compounds were identified by UPLC/Q-TOF-MS. The 15 propolis samples significantly differed in their total phenolic and total flavonoid contents, as well as their phytochemical profiles. The methanol extracts of propolis also showed significant anti-inflammatory effects in LPS-stimulated RAW 264.7 mouse macrophage cells at 10 μg propolis extract/mL concentration. Additionally, the propolis samples differed in their DPPH, ABTS cation, hydroxyl, and peroxide radical scavenging capacities and ferric reducing abilities. The results from this study may be used to improve the commercial production and consumption of Chinese propolis products.
研究领域:
植物学
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药理学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2020
·Vol. 43
·期 12
·2419-2428
·
IF 2.878
Xiaoling Yang,Yiduo Mi,Fang Liu,Jing Li,Haixiang Gao,Sanbing Zhang,Wenfeng Zhou,Runhua Lu
In this work, a novel extraction technique based on the effervescence-assisted dispersion and magnetic recovery of attapulgite/polypyrrole sorbents was developed for determining the concentrations of five pyrethroids in honey samples. The magnetic nanoparticles were synthesized by a one-pot method. Several experimental parameters that affected the extraction efficiency, including the dispersion conditions, pH, ionic strength, and desorption conditions, were investigated. Under optimal conditions, the calibration curves for the five pyrethroids in honey samples exhibited good linearity, with r 2 values ranging from 0.9979 to 0.9990. The limits of detection varied between 0.21 and 0.34 µg/L. Satisfactory recoveries of 81.42–106.73% with intra- and interday relative standard deviations of less than 6.94 and 10.89%, respectively, were obtained. Moreover, the sorbents exhibited acceptable batch-to-batch repeatability in the range of 5.06–15.01%, and each sorbent could be reused for up to four extraction cycles without a significant loss in the extraction recovery.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）