FOOD CHEMISTRY
·
2022
·
IF 9.231
Jiawen Ji,Changsheng Li,Bingjie Zhang,Wenjuan Wu,Jianli Wang,Jianhui Zhu,Desheng Liu,Rumin Gao,Yongqiang Ma,Sen Pang,Xuefeng Li
N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) and N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine-quinone (6PPDQ) can pose a threat to human health through the food chain because of their ubiquitous presence in the environment and the biotoxicity on organisms. In this work, we developed modified QuEChERS methods coupled with high performance liquid chromatography tandem mass spectrometry (HPLC/MS-MS) to explore whether 6PPD and 6PPDQ are present in fish and honey. The proposed methods showed acceptable limits of quantification (0.00043-0.001 mg/kg), linearity (R 2 > 0.99), recovery (73.3%-108.3%), matrix effect (70.4%-95.6%) and repeatability (RSD < 8.4%). Accordingly, 6PPD and 6PPDQ have been discovered in snakehead , weever and Spanish mackerel fish, while none of which have been detected in the honey samples. The results of our work contributed to increasing public attention to 6PPD and 6PPDQ in agricultural products and provided important reference for the analysis of them.
研究领域:
食品科学
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环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 1
·1-10
·
IF 5.833
Zheng Pimiao,Peng Tao,Wang Jianyi,Zhang Jing,Wang Zile,Zhang Yanfang,Ren Zhenhui,Wang Sihan,Jiang Haiyang
An ultrasensitive and rapid fluorescent immunoassay based on a broad-spectrum monoclonal antibody (mAb) was developed to detect pyrrolizidine alkaloids (PAs) in honey samples. First, Discovery Studio software was used to analyze and predict the target hapten, and retrorsine (RTS) was selected to react with succinic anhydride (HS) for hapten synthesization. A sensitive and broad-spectrum monoclonal antibody (mAb 13E1) was obtained for nine PAs. Then, fluorescent gold nanoclusters (AuNCs) were conjugated with mAb as a label probe and used in establishing a qualitative and quantitative lateral flow immunoassay (AuNCs-LFIA) for the determination of four PAs (retrorsine, platyphylline, senecionine, integerrimine) in honey within 14 min. The limits of detection (LOD) were 0.083 μg/kg. The recovery in spiked honey samples were 87.98–119.57%, with coefficients of variation of ≤ 11.5%. A total of 45 commercial import honey samples from nine different countries were tested through AuNCs-LFIA and UPLC-MS/MS method, and satisfactory consistency ( R 2 = 0.995) was obtained. The rates of positive samples were 55.56% (25/45), and the average concentrations of four PAs were 3.24–46.47 μg/kg. This ultrasensitive multi-PA method provides an alternative analytical tool for evaluating the human risk posed by the consumption of PA-contaminated honey. Graphical abstract
研究领域:
分析化学
·
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2015
·
IF 3.391
Chengzhu Ni,Binhe Zhu,Nani Wang,Muhua Wang,Suqing Chen,Jiajie Zhang,Yan Zhu
Honeydew is excreted by aphids as a sweet waste and nectar is floral honey. Honeydew and nectar are complicated samples which consist of various sugars and amino acids. In this work, a simple ion chromatography with column-switching method was developed for the simultaneous analysis of 8 monosaccharides and oligosaccharides in honeydew and nectar. A reversed-phase column was used as a pretreatment column to eliminate organics on-line and sugars were eluted from a collection loop to analytical column by using column-switching technique. This method showed good linearity ( r ⩾ 0.9994) and afforded low limits of detection ranging from 1.55 to 10.17 μg L −1 for all the analytes. Recoveries ranged from 95% to 105% and repeatability results were acceptable with relative standard deviation of less than 3.21% ( n = 6). This method was successfully applied to quantification of these sugars in honeydew and nectar. These results showed honeydew had much more oligosaccharides than nectar.
研究领域:
分析化学
·
植物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2014
·Vol. 406
·期 29
·7705-7714
·
IF 3.578
Huang X. Y.,Fang X. W.,Zhang X.,Dai X. M.,Guo X. L.,Chen H. W.,Luo L. P.
Herein, we constructed a platform of neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) for direct and rapid detection of chloramphenicol (CAP) in honey samples diluted with methanol. Under the optimized working conditions, the quantitative information of CAP residues was acquired effectively by EESI-Ion Trap MS n . Using heated methanol-N 2 as spray reagent, we reduced the limit of determination (LOD) from 73.3 ng/mL to 0.3 ng/mL, and the CAP detection is linear in the range of 1–5000 ng/mL ( R = 0.9947). For the honey samples with CAP of 10, 100, and 1000 ng/mL, the recoveries were 133.0, 80.6, and 101.1 %, and the relative standard deviations were 5.96, 8.82, and 8.71 %, respectively. The reproducibility assays showed the stability of this method. Therefore, this ND-EESI-MS method is powerful for direct, rapid, and quantitative CAP analysis in honey samples with high sensitivity, precision, and specificity.
In the current neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) method, N 2 is inlet into samples to desorb chloramphenicol (CAP). We tried to use some organic solvents as the spray reagent to dissolve CAP, and then the best neutral desorption efficiency was obtained when using methanol. We applied this modified ND-EESI-MS method to detect CAP in honey samples only with sample dilution. The limit of CAP detection was then reduced from 73.3 to 0.3 ng/mL, reaching the current EU standard. Therefore, this is a powerful method for direct, rapid, and quantitative CAP analysis in honey samples.
研究领域:
分析化学
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2017
·
IF 1.427
Yun Ma,Bing Zhang,Hongyan Li,Yulu Li,Jiangning Hu,Jing Li,Hongming Wang,Zeyuan Deng
Honey crystallization is a natural phenomenon commonly found in honey products. To better understand the characteristics of honey crystallization, the concentrations of the four major compositions (namely glucose, fructose, sucrose, and maltose) were determined in the liquid, crystal, and mixed forms of honey. Our study revealed the contents of glucose and fructose in three forms varied significantly (p < 0.05). Molecular dynamics simulation was performed to investigate honey crystallization process. All the simulation systems had a tendency to form a cubic crystal. When the ratio of glucose/fructose is 2.5:1, which is the same as the crystal found in honey, a shorter time and lower root-mean-square deviation were found compared to the ratios at 2:1, 1:1, and pure glucose. It infers that glucose/fructose at 2.5:1 is the most stable honey crystallization, relatively. The morphology of crystals found in honey, which was observed under an environment scanning electron microscope, looked like sticky balls with indistinct edges, different from that plates or granule of glucose crystal with flattened edges. These results indicated that the relative content of glucose and fructose had a significant effect on the crystallization of honey. The glucose/fructose at 2.5:1 probably is the critical ratio of honey crystallization, at which honey can form stable crystalline deposit speedily.
研究领域:
食品科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xiaofang Li,Lingling Guo,Liguang Xu,Liqiang Liu,Maozhong Sun,Xinxin Xu,Hua Kuang,Chuanlai Xu
The European Union (EU) has detected matrine (MAT) in honey and tea exported from China, which is neurotoxic, hepatotoxic, developmentally toxic, and reproductively toxic. To develop a rapid method for the detection of MAT in honey and tea, we designed and analyzed hapten in terms of structure-function similarity, surface electrostatic potential, 3D conformation, and Mulliken atomic charges, prepared a monoclonal antibody (mAb) with high sensitivity (IC 50 = 0.59 μg/L) and specificity to recognize MAT, and developed an immunosensor based on gold nanoparticles (GNP-based immunosensor) for the rapid qualitative detection of MAT in honey and tea samples with visual limit of detection (vLOD) of 2 and 5 μg/kg, respectively. The accuracy of the GNP-based immunosensor was verified by spiking recovery experiments. The GNP-based immunosensor is instrument-free and can be completed within 8 min with the advantages of convenience, rapidity and high sensitivity, which provides a tool for MAT monitoring of honey and tea in China.
研究领域:
食品科学
·
分析化学
·
毒理学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 18
·7625-7630
·
IF 1.821
Yi Li,Jinhui Zhou,Xiaofeng Xue,Liming Wu,Lanzhen Chen,Jinzhen Zhang,Shuming Yang
An optimization method of NBD-F derivatization has been developed for the determination of proline in honey using high performance liquid chromatography (HPLC) with fluorescence detection. To determine the free proline in different samples of honey, ultrasound extraction was performed using 0.1 M borate buffer solution as the extraction solvent. Chromatographic separation was performed on a XDB C18 column (150 × 4.6 mm i.d.) using 0.1 M sodium acetate buffer solution (pH 7.2) : methanol : tetrahydrofuran (900 : 95 : 5, respectively, v/v/v) as mobile phase A and methanol as mobile phase B at a flow rate of 1.0 mL min−1 with a run time of 10 min. The assay was linear over a range of 0.15 μg mL−1 to 100.00 μg mL−1 with a lower limit of detection of 3.00 mg kg−1, and recoveries were greater than 90%. The intra-day and inter-day precision was less than 4%. This method was successful in determining free proline in honey, ranging from 80.5 mg kg−1 to 426.4 mg kg−1 in different honey samples.
研究领域:
分析化学
·
食品科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yali Ding,Juanjuan Feng,Mingxia Sun,Yang Feng,Xubo Xin,Min Sun
The accurate detection of analytes in honey is affected by the complex substrates, making it crucial to employ an effective sample preparation technique. In this work, an imidazolium ionic liquid was functionalized to the silica surface by a click reaction for solid-phase extraction (SPE) column, and in situ anion-exchange process was performed with different organic anions (dodecyl sulfonate, dodecyl benzene sulfonate, and naphthalene sulfonate). These SPE columns were evaluated through extracting the estrogens. The naphthalene sulfonate-based SPE column displayed the best extraction ability among these, and it was combined with high-performance liquid chromatography-diode array detection to establish an online enrichment and analysis system. Under the optimal test conditions, an online analytical method was developed, with high enrichment factors (1872–4744), wide linear ranges (0.0033–1.50, 0.0165–1.50, and 0.0330–1.50 μg g −1 ), and low detection limits (0.001–0.010 μg g −1 ). The method successfully determined several estrogens in some honey samples, and achieved satisfactory recovery results.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Jiaojiao Xia,Bin Zou,Feng Liu,Pengyun Wang,Yan Yan
As a healthy and nutritious food, the taste of honey is usually affected by glucose crystals. To investigate the glucose concentration of honey, a new electroanalytical method based on a composite material of functionalized MWCNTs with β-cyclodextrin (β-CD/MWCNTs) was hereby proposed. After glucose oxidase (GOx) loading on β-CD/MWCNTs, a new biosensor GOx/β-CD/MWCNTs/GCE was constructed for the detection of glucose in honey. Scanning electron microscopy (SEM) and infrared spectroscopy (FT-IR) proved the successful immobilization of GOx on the β-CD/MWCNTs carrier. The prepared biosensor showed a linear response to the glucose concentration in the range of 50 μM ∼ 1.15 mM with the detection limit of 0.42 μM and a sensitivity of 32.28 μA mM −1 cm −2 was obtained. The biosensor has excellent stability (95.7 % after two weeks), reproducibility (n = 10, relative standard deviation (RSD) = 2.5 %) and anti-interference. HPLC results validated the accuracy of the newly proposed method in the detection of glucose in real honey samples.
研究领域:
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Xiao-Hua Zhang,Xiang-Dong Qing,Shu-Ting Mu,Deguo Wang,Jing-Jing Zheng,San-Jiu Zhou,Chao Kang,Zhi Liu
The chemometric second-order multivariate calibration method was applied to the qualitative and quantitative analysis of seven phenolic acids in honey samples with five different floral origins. First, based on the “second-order advantage,” reasonable resolutions and satisfactory quantitative results were obtained even in the presence of strong background interference and overlapping peaks in complex real honey samples. Average spike recoveries for the seven target analytes were in the range of 89.34–118.81%, with high correlation coefficient ( r ≥ 0.9997), low limit of quantification (1.00–20.20 ng mL −1 ) and high sensitivity (0.135–0.375 mL ng −1 ). Second, the quantitative data of seven target analytes were analyzed by principal component analysis (PCA) on MATLAB software to classify honey of five nectar sources. These results indicated that there is a strong association between the content of major phenolic acids and the flower sources of honey. Third, the quantitative composition-antioxidant activity relationship analysis was evaluated by partial least squares (PLS) analysis and studied to provide reliable analysis of multi-marker antioxidant data. The results enable the continued exploitation and utilization of honey from different floral origins and further contribute to honey market development and choices in available honey for consumption.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Lu Xu,Daowang Lu,Qiong Shi,Hengye Chen,Shunping Xie,Gangfeng Li,Hai-Yan Fu,Yuan-Bin She
To enhance the power of untargeted detection, a “turn-off” fluorescent probe with double quantum dots (QDs) was developed and coupled with chemometrics for rapid detection of multiple adulterants in an herbal ( Rhus chinensis Mill., RCM) honey. The double water-soluble ZnCdSe-CdTe QDs have two separate and strong fluorescent peaks , which can be quenched by honey and extraneous adulterants with varying degrees. Class models of pure RCM honey samples collected from 6 different producing areas ( n = 122) were developed using one-class partial least squares (OCPLS). Four extraneous adulterants, including glucose syrup, sucrose syrup, fructose syrup, and glucose-fructose syrup were added to pure honey samples at the levels of 0.5% to 10% (w/w). As a result, the OCPLS model using the second-order derivative (D2) spectra could detect 1.0% (w/w) of different syrups in RCM honey, with a sensitivity of 0.949. The double water-soluble QDs, which can be adjusted for analysis of other water-soluble food samples, has largely extended the capability of traditional fluorescence and will provide a potentially more sensitive and specific analysis method for food frauds.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 15
·21826-21838
·
IF 4.223
Pang Xiuyu,Li Chenyu,Zang Chuanjiang,Guan Lei,Zhang Peng,Di Chunxiang,Zou Nan,Li Beixing,Mu Wei,Lin Jin
An analytical method of simultaneous detection of ten insecticide residues in honey and pollen was established. The samples were purified with QuEChERS approach using new adsorbents and analyzed with UPLC-MS/MS. The results showed that both of graphene and carbon nanotubes were highly efficient adsorbents for the dSPE clean up to eliminate coextractives in the samples, and graphene was superior to carbon nanotubes for the detection of pesticide residues in honey and pollen samples. The proposed method was used to detect pesticide residues in 25 honey samples and 30 pollen samples which were randomly collected from more than ten provinces in China. All honey samples contain 1–27 μg/kg of chlorpyrifos residues. Only 4% of the honey samples were detected containing acetamiprid and imidacloprid, while the other seven pesticides were not detected. Chlorpyrifos residues were found in all pollen samples (5–66 μg/kg), among which twenty percent exceeded the maximum residue limits (MRLs, 50 μg/kg, European Commission Regulation). Most of the pollen samples containing pesticide concentrations higher than MRLs were collected from rape, followed by lotus, camellia, and rose. Besides, 36.7% and 33.3% of the pollen samples had imidacloprid and flupyradifurone higher than 5 μg/kg. A total of 26.7% pollen samples were detected containing bifenthrin, while none of the other six pesticides were detected in pollen samples.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）