JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2020
·
IF 3.306
Haochi Liu,Jie Ding,Ligang Chen,Lan Ding
A novel approach was proposed to prepare carbon dots (CDs) by acid carbonization and hydrothermal treatment of pork rib bones from food waste. The synthesized animal waste biomass CDs have the merits of eco-friendly, superior fluorescence properties and biocompatibility. The fluorescence of animal waste biomass CDs could be quenched efficiently by dithizone due to the coordination effect. With the addition of dimethoate, the dithizone ligands at CDs are replaced by hydrolysates, and hence the fluorescence is recovered. Thus, a sensing platform was constructed based on the coordination-originated fluorescence of animal waste biomass CDs which can sensitively and selectively detect dimethoate due to a simple displacement mechanism. Upon optimal conditions, the detection limit of dimethoate is 0.064 μM. The linear ranges were obtained in a range of 0.15 μM–5.0 μM. The method is rapid, convenient and environmentally acceptable that can directly detect dimethoate in water samples with recoveries of 93 %–105 %, and the relative standard deviations (RSDs) were 2 %–6 %, respectively. This simple strategy can be used to develop the fluorescence recovery sensors for chemical detection.
研究领域:
分析化学
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环境科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Lingqian Bai,Zhihua Gao,Xiaofeng Xu,Wenxia Lv,Yikui Wang,Kexin Dong,Zhijun Yu,Xiaolong Yang
Haemaphysalis longicornis is a notorious vector tick species widely distributed in China, with its current control relying heavily on synthetic chemicals. The disadvantages of chemical acaricides including single mechanism of action, environmental pollution, and easy-to-induce acaricidal resistance have long been criticized. As plant extracts and essential oils (EOs) have been proven to show good insecticidal activity and environmental safety, hence, in this study, the chemical constituents of EOs from Cinnamomum cassia and Cinnamomum camphora were analyzed and their potential for application in the control of H. longicornis was explored. Gas chromatography–mass spectrometry analysis showed that the Trans -cinnamaldehyde and Diethyl phthalate were the main components of the EOs of C. cassia and C. camphora , respectively. Larval packet test and nymph immersion test showed that the EOs of C. cassia and C. camphora had significant acaricidal activity against unfed larvae and nymphs of H. longicornis , and the EO of C. cassia showed more toxic against unfed adults than C. camphora EO at the same concentration. Enzyme assays revealed that the C. cassia EO significantly inhibited the activity of acetylcholinesterase (AchE), and C. camphora EO and Trans -cinnamaldehyde had no significant effect on AchE. Cinnamomum cassia EO and C. camphora EO significantly inhibited the activity of carboxylesterase (CarE). Trans -cinnamaldehyde significantly inhibited the activity of catalase (CAT), and C. cassia EO and C. camphora EO had no obvious effect. The above results suggested that the EOs of C. cassia and C. camphora and their major components target different enzymes, which may potentially mitigate the acaricidal resistance development in tick, indicating the prospect that these EOs may become an effective substitute for chemical synthetic acaricides in tick control.
研究领域:
昆虫学
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药理学
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植物科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PEST SCIENCE
·
2023
·
IF 4.8
Nwanade Chuks Fidelis,Wang Min,Yu Zhijun,Liu Jingze
To develop an appropriate program for essential oils (EOs) used in pest control, it is important to acquire information about the effect of the EO when used at a low concentration. Previously, we demonstrated the enzymatic activity of Haemaphysalis longicornis (the main livestock pest in Australia and New Zealand) exposed to Cinnamomum cassia EO and ( E )-cinnamaldehyde (its major compound) when applied at 50% lethal concentration (LC 50 ). However, H . longicornis respond to toxic substances via different mechanisms and at different times of exposure. Herein, the enzymes involved in the defense response were studied by monitoring glutathione-S-transferase, acetylcholinesterase, and Na + /K + -adenosine triphosphatases (ATPase) activities in H . longicornis larvae exposed to C . cassia EO and ( E )-cinnamaldehyde across different times. Further, the molecular changes related to the defense mechanisms were studied by monitoring adenosine triphosphate (ATP)-binding cassette (ABCB10) gene expression. In addition, we investigated the acaricidal activity of the test substances against the engorged larvae and nymphs of H . longicornis . Our results showed that the activities of these enzymes were significantly affected by the EO and/or ( E )-cinnamaldehyde depending on the exposure time. We observed up-regulation of the ABCB10 gene in the larvae. Also, our results revealed that C . cassia EO (LC 50 = 1.46 and 16.69 mg/mL) and ( E )-cinnamaldehyde (LC 50 = 3.86 and 17.96 mg/mL) were effective against the engorged larvae and nymphs, respectively. Our results provided a deeper understanding of the defense mechanisms of H . longicornis, which may provide an important clue for the development of EO-based acaricides that are cheaper and environmentally friendly.
研究领域:
昆虫学
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分子生物学
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害虫防治
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兽医学
✓ 已验证
产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Yuanzhao Wu,Tianyi Chen,Yumei Xia,Jiawen Wang,Anli Wang,Binjie Wang,Jiye Wang,Weixuan Yao
Methidathion is a highly effective organophosphorus pesticide and is extensively utilized for the control of insects in agricultural production. However, there is little information on the adverse effects and underlying mechanisms of methidathion on aquatic organisms . In this work, embryonic zebrafish were exposed to methidathion at concentrations of 4, 10, and 25 mg/L for 96 h, and morphological changes and activities of antioxidant indicators alterations were detected. In addition, the locomotor behavioral abilities of zebrafish exposed to methidathion were also measured. To further explore the mechanism of the toxic effects of methidathion, gene expression levels associated with cardiac development, cell apoptosis, and the immune system were tested through qPCR assays. The findings revealed that methidathion exposure could induce a decrease in survival rate, hatchability, length of body, and increase in abnormality of zebrafish, as well as cardiac developmental toxicity . The LC 50 value of methidathion in zebrafish embryos was determined to be about 30.72 mg/L at 96 hpf. Additionally, methidathion exposure triggered oxidative stress in zebrafish by increasing SOD activity, ROS, and MDA content. Acridine orange (AO) staining indicated that methidathion exposure led to apoptosis, which was mainly distributed in the pericardial region. Furthermore, significant impairments of locomotor activity in zebrafish larvae were induced by methidathion exposure. Lastly, the expression of pro-inflammatory factors including IFN-γ, IL-6, IL-8, CXCL-clc, TLR4 , and MYD88 significantly up-regulated in exposed zebrafish. Taken together, the results in this work illustrated that methidathion caused developmental toxicity, cardiotoxicity, and immunotoxicity in embryogenetic zebrafish.
研究领域:
环境科学
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毒理学
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水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2024
·
IF 5.7
Wu Huimei,Jmel Mohamed Amine,Chai Jinwei,Tian Maolin,Xu Xueqing,Hui Yuan,Nandakumar Kutty Selva,Kotsyfakis Michail
Protease inhibitors regulate various biological processes and prevent host tissue/organ damage. Specific inhibition/regulation of proteases is clinically valuable for treating several diseases. Psoriasis affects the skin in the limbs and scalp of the body, and the contribution of cysteine and serine proteases to the development of skin inflammation is well documented. Cysteine protease inhibitors from ticks have high specificity, selectivity, and affinity to their target proteases and are efficient immunomodulators. However, their potential therapeutic effect on psoriasis pathogenesis remains to be determined. Therefore, we tested four tick cystatins (Sialostatin L, Sialostatin L2, Iristatin, and Mialostatin) in the recently developed, innate immunity-dependent mannan-induced psoriasis model. We explored the effects of protease inhibitors on clinical symptoms and histological features. In addition, the number and percentage of immune cells (dendritic cells, neutrophils, macrophages, and γδT cells) by flow cytometry, immunofluorescence/immunohistochemistry and, the expression of pro-inflammatory cytokines (TNF-a, IL-6, IL-22, IL-23, and IL-17 family) by qPCR were analyzed using skin, spleen, and lymph node samples. Tick protease inhibitors have significantly decreased psoriasis symptoms and disease manifestations but had differential effects on inflammatory responses and immune cell populations, suggesting different modes of action of these inhibitors on psoriasis-like inflammation. Thus, our study demonstrates, for the first time, the usefulness of tick-derived protease inhibitors for treating skin inflammation in patients.
研究领域:
皮肤病学
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免疫学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
·
IF 4.6
Jia Wei,Yi Zhang,Wangui Peng,Weihong Huang,Yunfei Cao,Wenming Yang,Wanzhen Xu
Pesticide residues in food pose significant risks to human health, necessitating accurate monitoring methods. To address this, a lattice distortion strategy-based highly sensitive photoelectrochemical (PEC) sensor was developed for profenofos (PFF) detection. Substitution of Bi 3 + with Co 2+ in the Bi 2 MoO 6 lattice induced enhanced lattice distortions and oxygen vacancies, reducing the bandgap and dramatically improving visible-light absorption and charge transfer efficiency. These modifications synergistically boosted PEC performance, with 4 % Co-doped Bi 2 MoO 6 achieving optimal results, including a reduced narrowed bandgap (2.28 eV) and a significantly enhanced photocurrent density compared to pristine Bi 2 MoO 6 . The sensor exhibited a broad linear detection range (0.5 mg L −1 to 0.01 ng L −1 ) and an ultralow detection limit of 3.3 pg L −1 , demonstrating excellent sensitivity and selectivity in real food samples. This work presents an innovative efficient, non-toxic, and eco-friendly tool for pesticide residue detection, paving new avenues for food safety monitoring and public health protection.
研究领域:
食品科学
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环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
·
IF 3.571
Pingyue Wang,Huanhuan Li,Md Mehedi Hassan,Zhiming Guo,Zheng-Zhu Zhang,Quansheng Chen
In this study, a highly sensitive upconversion fluorescence (FL) biosensor was developed for the detection of organophosphorus pesticides (OPs) based on an acetylcholinesterase (AChE) modulated FL “off–on–off” strategy. The luminescence of synthesized UCNPs could be quenched strongly by Cu2+ due to an energy transfer effect. Upon addition of AChE and acetylthiocholine (ATCh), the enzymatic hydrolysate (thiocholine) could seize Cu2+ from UCNPs-Cu2+ mixture, resulting in the quenched FL triggered on. OPs could irreversibly impede the activity of AChE, which caused the formation of thiocholine to decrease, thus, reduced the recovery of FL. Under the optimum conditions, a linear detection range from 0.1 to 50 ng/mL was achieved for the representative OPs (diazinon) with LOD of 0.05 ng/mL. Furthermore, the ability of the biosensor to detect OPs was also confirmed in adulterated environmental and agricultural samples. In validation analysis, the proposed sensor showed satisfactory results (p > 0.05) with GC–MS.
研究领域:
生物传感技术
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食品安全检测
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Xinyi Feng,Yuqing Fu,Zhanqiu Tang,Hongyuan Tian,Zhenglong Wu,Weidong Qin
Dichlorvos and Cr(VI) are widespread environmental contaminants that pose significant risks to water quality, ecosystems, and human health. Therefore, the development of rapid, sensitive, and reliable detection methods for these pollutants is critically important. Although various techniques have been employed for the individual detection of these substances, achieving simultaneous and selective detection of both remains a considerable analytical challenge. In this study, iron-doped poly(amidoamine) carbon dots (PAMAM-Fe CDs) were synthesized, exhibiting multiple enzyme-mimetic activities, including peroxidase-, oxidase-, superoxide dismutase-, and catalase-like functions. Notably, dichlorvos and Cr(VI) were found to selectively influence the catalase-like and oxidase-like activities, respectively. Based on these findings, a dual-mode fluorescence and colorimetric sensing platform was developed, enabling rapid, sensitive, and selective detection of dichlorvos and Cr(VI), with detection limits of 1.69 × 10⁻⁵ µg/mL and 0.0418 µM, respectively. The applicability of this platform was validated through analysis of real-world samples, demonstrating satisfactory recovery rates and reproducibility. This work not only establishes a new approach for detecting dichlorvos and Cr(VI) for food safety and environmental monitoring but also provides new insights into designing multienzyme-active nanomaterials with significant practical potential.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Yao Chen,Yanyan Zhu,Yuhui Zhao,Jing Wang
An efficient and convenient detection method for organophosphorus pesticide (OP) residues is needed because of their high neurotoxicity and severe threat to food safety. OPs effectively reduce the production of thiocholine in the acetylcholinesterase/acetylthiocholine reaction by inhibiting the activity of acetylcholinesterase. Therefore, we developed a feasible and convenient fluorescent and colorimetric dual-response sensor based on the competitive complexation of Cu 2+ between graphitic carbon nitride nanosheets and thiocholine for the rapid detection of OPs with high sensitivity. Malathion was used as a model OP, and a linear range of 70–800 nM with a detection limit of 6.798 nM for a fluorescent signaling platform and 2.5–25 nM with a detection limit of 1.204 nM for a colorimetric probe were attained. The constructed probe was successfully applied to determine OP in actual samples of cabbages leaves and tap water. The results indicated that the dual-response probe was reliable and sensitive to actual samples.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jun-feng Qi,Duo Tan,Xue-juan Wang,Hui-ting Ma,Ying-chun Wan,Ao Hu,Lin-hai Li,Bin Xiao,Bin Lu
Biosensors using acetylcholinesterase (AChE) as the recognition element detect both toxicity (AChE inhibition activity) and concentration of organophosphorus pesticides (OPs). However, real application of these biosensors remains a challenge due to their low selectivity and low resistance to sample matrix, interferences and hash analytical conditions. In this study, a novel dual-recognized strategy was established by using molecularly imprinted polymer (MIP) and AChE selective to different moieties of acephate (AP) as affinity molecules simultaneously. Using AP as the model OP, MIP highly selective to certain moieties but not phosphorus of AP was prepared on the surface of microplate by self-polymerization of dopamine. The MIP enriched AP from samples selectively. Then AChE selective to phosphorus of AP quantified the levels of AP captured by MIP. AP captured by MIP still inhibited the activity of AChE because the affinity of AP to AChE was higher than to MIP. By incorporating selective sample pretreatment with AChE inhibition assay in one sensor, the biosensor identified AP from other OPs, carbamates pesticides and interferences commonly found in food and environmental samples. When vegetables with high matrix effect on AChE inhibition assay were analyzed, selective sample pretreatment ensured the biosensor to detect AP more accurately and selectively than classical AChE inhibition assay. The results of the biosensor had no statistical difference compared with those of HPLC-MS/MS. The dual-recognized strategy may provide a new solution to detect target enzyme inhibitors in complicated samples using enzyme-based biosensors selectively and accurately.
研究领域:
生物传感技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 33
·期 9
·2048-2052
·
IF 3.223
Lei Liu,Jiawei Guo,Longhua Ding
An electrochemical sensor based on raffia derived porous carbon (RPC) and polyaniline (PANI) composite functional glass carbon electrode (GCE) was constructed for imidacloprid (IMI) determination. PANI nanowire arrays were deposited on RPC surface uniformly without aggregations. The electrochemical response of IMI at RPC@PANI/GCE is about four times than that at bare GCE, indicating high electrocatalytic activity of RPC@PANI towards IMI reduction. The prepared sensor also offers a wide linear range of 0.1–70 μg mL −1 for IMI determination with a limit of detection (LOD) of 0.03 μg mL −1 . In addition, it offers high recoveries with testing real samples.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Guoxin Wu,Chuanqi Zhang,Chang Liu,Xiangzi Li,Yuanyuan Cai,Meifang Wang,Deqiang Chu,Liyun Liu,Tian Meng,Zhiming Chen
Organothiophosphate pesticides (OPPs) are the most common water contaminants, significantly endangering human health and bringing serious public safety issues. Thus, developing effective technologies for the removal or trace detection of OPPs from water is urgent. Herein, a novel graphene-based silica-coated core–shell tubular magnetic nanocomposite (Ni@SiO 2 -G) was fabricated for the first time and used for the efficient magnetic solid-phase extraction (MSPE) of the OPPs chlorpyrifos , diazinon , and fenitrothion from environmental water. The experimental factors affecting extraction efficiency such as adsorbent dosage, extraction time, desorption solvent, desorption mode, desorption time, and adsorbent type were evaluated. The synthesized Ni@SiO 2 -G nanocomposites showed a higher preconcentration capacity than the Ni nanotubes , Ni@SiO 2 nanotubes, and graphene. Under the optimized conditions, 5 mg of tubular nano-adsorbent displayed good linearity within the range of 0.1–1 μg·mL –1 , low limits of detection (0.04–0.25 pg·mL –1 ), low limits of quantification (0.132–0.834 pg·mL −1 ), good reusability (n = 5; relative standard deviations between 1.46% and 9.65%), low dosage (5 mg), and low real detection concentration (< 3.0 ng·mL −1 ). Moreover, the possible interaction mechanism was investigated by density functional theory calculation. Results showed that Ni@SiO 2 -G was a potential magnetic material for the preconcentration and extraction of formed OPPs at ultra-trace levels from environmental water.
研究领域:
环境科学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）