ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2023
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IF 6.8
Guang-Li Fu,Qin-Yuan Meng,Yu Chen,Jin-Zhao Xin,Jia-Hui Liu,Wei Dang,Hong-Liang Lu
The antidiabetic pharmaceutical metformin (MET) is largely unmetabolized by the human body. Its residues are readily detectable in various aquatic environments and may have adverse impacts on the growth and survival of aquatic species. To date, its toxicological effects have scarcely been explored in non-fish species. Here, we exposed the tadpoles of black-spotted pond frog ( Pelophylax nigromaculatus ) to different concentrations (0, 1, 10 and 100 μg/L) of MET for 30 days and measured the body size, intestinal microbiota and metabolites to evaluate potential effects of MET exposure in amphibian larvae. MET exposure did not affect the growth and intestinal microbial diversity of tadpoles. However, intestinal microbial composition changed significantly, with some pathogenic bacteria (e.g., bacterial genera Salmonella , Comamonas , Stenotrophomonas , Trichococcus ) increasing and some beneficial bacteria (e.g., Blautia , Prevotella ) decreasing in MET-exposed tadpoles. The levels of some intestinal metabolites associated with growth and immune performance also changed significantly following MET exposure. Overall, our results indicated that exposure to MET, even at environmentally relevant concentrations, would cause intestinal microbiota dysbiosis and metabolite alteration, thereby influencing the health status of non-target aquatic organisms, such as amphibians.
研究领域:
生态毒理学
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环境科学
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微生物学
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PubMed
高影响因子
AQUATIC ECOLOGY
·
2018
·Vol. 53
·期 1
·9-19
·
IF 1.978
Zhao Tian,Wang Xiaoyi,Wang Xungang,Wang Sishuo,Chen Youhua,Jiang Jianping
As one of the important contributors of biodiversity, amphibian populations are declining worldwide. Numerous factors are involved in these declines, one of them being the use of fertilizers in agriculture. This is especially true for tadpoles which can live in the fertilizer-polluted farmland water bodies until metamorphosis. The present study aimed to assess the effects of urea (CH 4 N 2 O), as one of the most economical and effective fertilizers, on the anti-predator behavior and intraspecific functional trait variability of Asiatic toad ( Bufo gargarizans ) tadpoles. Based on published literatures and the field observation of urea concentrations in China, glass beakers with a gradient of urea concentrations (0, 200, 400, 600, and 1200 mg/L) were prepared, with 10 tadpoles placed in each glass beaker. Each treatment was replicated three times. Mosquito fish ( Gambusia affini s) cues were used as the predator disturbance, and three main functional traits (body mass, trunk bending shape, and eye position) were selected. Our results revealed that tadpoles activity levels decreased when exposed to urea as well as to mosquito fish cues. However, urea exposure did not alter the anti-predator behaviors of tadpoles. Additionally, we found that increasing urea concentrations might modify some functional traits of tadpoles. Importantly, urea disturbance decreased tadpoles intraspecific functional trait variability. (Functional similarity increased between developmental stages.) Given that functional similarity between developmental stages could potentially increase intraspecific competition, urea could indirectly reduce tadpoles survival by decreasing intraspecific traits variability.
研究领域:
生态学
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环境科学
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高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
·
IF 6.2
Zihan Li,Youhua Chen
Polylactic acid microplastics (PLA-MPs), biobased plastics made from renewable resources, are considered to be a potential solution for alleviating the pollution pressure of plastics; however, the potential environmental risks of PLA-MPs must be further evaluated. In this study, the effects of PLA-MPs on the tadpoles of Pelophylax nigromaculatus were investigated by designing different PLA-MP exposure experiments. We found that PLA-MPs negatively affected the survival, growth and development of tadpoles. In addition, in open field tests, PLA-MPs also reduced tadpole locomotion while resulting in more repetitive searching behavior within a restricted area. This effect was more pronounced at higher concentrations of PLA-MPs (20 mg/mL) and in combination with the heavy metal Cd 2+ . In the tank tests, PLA-MPs increased tadpole aggregation, and their combined effect with Cd 2+ resulted in a tendency for tadpole aggregation to increase and then decrease, with the distribution tending to favor aggregation in edge regions. PLA-MPs also strongly inhibited the spatiotemporal exploratory activities of tadpoles in the tanks. This study provides a more detailed investigation of the behavioral effects of PLA-MPs on tadpoles and provides a theoretical basis for subsequent ecotoxicological studies of PLA-MPs.
研究领域:
环境科学
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动物学
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PubMed
高影响因子
AQUACULTURE
·
2023
·
IF 4.5
Juan Gao,Xueshan Li,Kangle Lu,Kai Song,Jian Zhang,Ling Wang,Chunxiao Zhang
This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of the bullfrog ( Lithobates catesbeianus ) tadpoles. Seven isolipidic and isoenergetic diets were formulated with 24.61%, 28.49%, 33.74%, 37.50%, 44.06%, 48.39% and 53.92% crude protein. Triplicate replicates of tadpoles (growth stage 25 and 0.009 g) were fed with experimental diets two times daily for 11 weeks, and the daily feeding amount was 6.50% of the tadpole's body weight. Weight gain (WG), specific growth rate (SGR), the total antioxidant capacity (T-AOC) and catalase (CAT) activity in the liver, the intestinal trypsin activity (TPS) and metamorphosis rate at stage 42 of bullfrog tadpoles all increased with increasing dietary protein level from 24.61% to 44.06% and then decreased. Based on the second-order polynomial regression analysis of the weight gain and metamorphosis rate at stage 41 vs. dietary protein level, the optimal dietary protein level for bullfrog tadpoles was 42.49% and 46.50%, respectively. In conclusion, the optimum dietary protein level of bullfrog tadpoles was estimated to be 42.49%–46.50% of the diet.
研究领域:
水产养殖
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营养学
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动物生理学
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高影响因子
Comparative Biochemistry and Physiology D-Genomics & Proteomics
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2021
·
IF 2.674
Liming Chang,Bin Wang,Meihua Zhang,Jiongyu Liu,Tian Zhao,Wei Zhu,Jianping Jiang
Corticosterone (CORT)-mediated adaptive plasticity improves animal fitness in stressful environments. Although it brings ecological benefits, the cost potentially constrains its expression and evolution. Revealing the factors affecting plasticity costs is of great ecological and evolutionary significance. Evidence indicates that both CORT and background colour can induce metabolic changes in animals, which in turn determine phenotypic plasticity . However, whether and/or how CORT and background colour jointly act on plastic responses has not been studied. Here, this question has been investigated in amphibian tadpoles ( Microhyla fissipes ) exposed to CORT at different background colours (white or black) using integrated morphological, histological, and transcriptomic analyses. The results showed that CORT exposure increased relative tail length, immune function, and metabolic maintenance (i.e., transcription of substrate catabolism and oxidative phosphorylation) at the expense of reduction in growth rate and skin melanin level. The black background also increased relative tail length and metabolic maintenance (i.e., transcription of oxidative phosphorylation) at the cost of reduction in growth rate, but increased skin melanin level. The expression of critical pigmentation genes indicated that black background activated a distinct and opposite pigmentation regulating route to CORT. Although there was no interactive effect of background colour and CORT on phenotypic and metabolic variations, their additive effects further impact the trade-off between somatic growth, metabolic maintenance, and pigmentation in terms of resource allocation. In conclusion, the individual and additive effects of background colour and CORT exposure on tadpole plasticity were revealed. These results likely provide new insights into the environmental adaptation of animals.
研究领域:
生态学
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进化生物学
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生理学
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PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
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2016
·Vol. 27
·期 10
·1376-1382
·
IF 1.823
Naipu He,Yue Wang,Zhenwu Lu
The temperature-responsive poly (N, N-diethylacrylamide) (pDEAAm) with narrower molecular weight distribution was prepared by the atom transfer radical polymerization and characterized by 1 HNMR and gel permeation chromatography. The temperature-responsive “tadpole-shaped” BSA–pDEAAm hybrids were fabricated via a free Cys-34 residue of bovine serum albumin (BSA) site specifically binding to the end group disulfide bonds of pDEAAm and characterized by native-polyacrylamide gel electrophoresis (Native-PAGE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry. Their temperature-responsive behaviors were measured by ultraviolet-visible spectra (UV-Vis). The lower critical solution temperature (LCST) of the pDEAAm was identified as 28°C, and the LCST of BSA–pDEAAm hybrids was identified as 31°C. The morphologies of BSA–pDEAAm hybrids self-assembled in the aqueous solutions with two different temperatures at 25 °C and 40°C were investigated by transmission electron microscopy. Below the LCST of BSA–pDEAAm hybrids, the separate spherical nanoparticles were observed. In contrast, bundles and clusters were observed above the LCST of BSA–pDEAAm hybrids. The results suggested that the self-assembly morphology of BSA–pDEAAm hybrids depended upon the pDEAAm block in BSA–pDEAAm hybrids, and the morphology transitions were effected by the LCST of BSA–pDEAAm hybrids. It would be expected to be used in biomedicine and materials science. Copyright © 2016 John Wiley & Sons, Ltd.
研究领域:
高分子化学
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材料科学
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生物医学工程
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高影响因子
POLYMER
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2017
·
IF 3.684
Yuanyuan Tao,Hanying Zhao
Shell-sheddable polymeric assemblies are a type of self-assembled structures formed by amphiphilic block copolymers with cleavable linkages at the junction points. The shell-sheddable self-assemblies have been used as nanocarriers for drugs and inorganic nanoparticles . In this research, synthesis and self-assembly of amphiphilic tadpole-shaped block copolymer with disulfide bonds at the junction points were investigated. Dialkyne-terminated polystyrene (Da-PS) was synthesized by reversible addition-fragmentation chain transfer polymerization and thiol-disulfide exchange reaction. The tadpole-shaped block copolymer was synthesized by bimolecular coupling between azido-terminated PEG and Da-PS. Size exclusion chromatograph , FTIR, 1 H NMR and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass analysis results all indicated successful synthesis of the polymer. The tadpole-shaped block copolymer self-assembles into vesicles in aqueous solution. The disulfide bonds at the junction points can be cleaved by a reducing agent, resulting in aggregation of the assemblies.
研究领域:
材料科学
·
药物递送系统
·
高分子化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Linbin Zheng,Meng Li,Zhouyu Jiang,Junyan Fan,Zengbo Fang,Jihuai Zheng,Yan Cui
Background The increased production and use of chiral pesticides will enhance their exposure in the environment. Chiral pesticides typically exhibit varied biological effects among these enantiomers. Therefore, it is very essential to develop and validate chiral analytical methods to investigate their potential ecological risks from a stereoselective perspective. Current separation of pesticides enantiomers relies extensively on chiral stationary phases (CSPs), while the development of β-Cyclodextrin derivatives CSPs become the research focus due to their great modifiability and excellent chiral recognition capabilities. Results A novel chiral stationary phase, 3,5-dichlorophenylaminomethyl-6-phenylenediamine-β-cyclodextrin chemically bonded silica gel (MPDCDA), was successfully prepared. Based on that, a stereoselective HPLC-MS/MS method was developed and validated for the determination of tebuconazole enantiomers in Rana nigromaculata tadpoles. After extraction by QuEChERS, the tebuconazole enantiomers were completely separated with the resolutions of 1.63 using the mobile phase of methanol-water (70/30, v/v). Good linearity (r > 0.9990) for both enantiomers over a concentration range of 0.20–500.0 ng/mL was obtained with the accuracy ranged from 6.7 % to 9.3 % and the intra-day and inter-day precisions below 6.2 % at three quality control levels. The proposed method was successfully applied in evaluating the enantioselective bioaccumulation and elimination profiles of tebuconazole in tadpoles. At the tested conditions, there was no significantly enantioselective difference in the bioaccumulation process for S - tebuconazole and R -tebuconazole. However, the elimination process of tebuconazole enantiomers was enantioselective with R -tebuconazole preferentially degraded. Significance This work provided an accurate risk assessment of chiral pesticides to non-target aquatic organisms from a stereoselective perspective. These findings would deepen our understanding of the potential ecological risks of chiral pesticides on aquatic organisms and provide scientific support for the protection of aquatic organisms and their ecological environments, as well as sustainable development.
研究领域:
分析化学
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环境科学
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生态毒理学
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PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2017
·
IF 3.743
Peng Xu,Ledan Huang
Populations of many amphibian species are declining worldwide in part because of pesticide contamination. As a surface water contaminant, α-cypermethrin may have severe ecological impacts on amphibians. Here, we examined the acute toxicity of α-cypermethrin enantiomers to dark-spotted frog Rana nigromaculata tadpoles at 24, 48, 72 and 96 h, finding that the tadpoles were indeed sensitive to α-cypermethrin. The (S)-(1R, 3R)-enantiomer was approximately 29 times more toxic than the (R)-(1S, 3S)-enantiomer at 96 h. A significant delayed growth in R. nigromaculata tadpoles after exposure to 0.5 µg L −1 of S-(1R, 3R)-cypermethrin was observed. Additionally, increased superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) and malondialdehyde (MDA) levels indicate the presence of oxidative stress in the tadpoles. Further, tadpoles exposed to sublethal concentrations of α-cypermethrin enantiomers exhibited enantioselective growth and oxidative damage. Bioaccumulation experiments showed that the tadpoles could rapidly accumulate α-cypermethrin. The (R)-(1S, 3S)-enantiomer was preferentially accumulated over the (S)-(1R, 3R)-enantiomer, and it was also eliminated more quickly, as evidenced in the subsequent depuration experiments.
研究领域:
环境科学
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毒理学
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生态学
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生物化学
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PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2020
·
IF 7.183
Biaowen Wei,Jun Feng,Caidi Chen,Shixi Zhong,Shijun Liao,Yigang Yu,Xiuhua Li
A series of tadpole-type ionic ionomers PPO-DMODAs with long alkyl side chains designed for electrodialysis (ED) desalination were synthesized successfully from dimethyloctadecylamine (DMODA) and brominated poly (2,6-dimethyl-1,4-phenylene oxide) (BPPO). The series of AEMs display stably low water uptakes ranging from 6.2% to 15.1% and high dimensional stabilities with swelling rations of 1.9%–4.8% at temperatures of 25 °C–60 °C. The nano-phase separated tadpole-type ionic structures ensure that the PPO-DMODAs membranes show excellent permselectivities with values above 94.6% and outstanding ED performances in the mostly reported desalination system of NaCl aqueous solutions. Especially, PPO-DMODA-3 surpasses commercial membrane TWEDA1 in ED competitions and ED-stability matches with current efficiency (η) of 88.7%, salt flux (J) of 80.61 mg m −2 s −1 , energy consumption (EC) of per kg of NaCl of 2.56 kWh kg −1 and η retentions of the repeated runs higher than 97% of that of the origin run. Moreover, PPO-DMODA-3 membrane obtained from the ethanol solution exhibits stable aggregate structure and ED performances comparable to those of the PPO-DMODA-3 membranes cast with NMP and TCE solutions. Good solubility of PPO-DMODAs in ethanol offers an environmentally friendly green method to fabricate ED AEMs. In consideration of the advantages of low cost, simple synthesis process and green membrane casting technology, PPO-DMODA-3 membrane has a strong potential for ED-desalination application.
研究领域:
材料科学
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环境工程
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化学工程
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高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 12
·2877-2884
·
IF 6.331
Yanping Cao,Shengjie Liu,Zhaoqiang Wu,Hong Chen
Linear poly(N-hydroxyethylacrylamide) (PHEAA) is regarded as one of the most promising antifouling materials because of its excellent antifouling properties and good hemocompatibility. However, the antifouling performance of topological PHEAAs remains largely unknown. Herein, the preparation of antifouling surfaces based on a tadpole-shaped PHEAA coating is reported for the first time, and how the tadpole-shaped PHEAA architecture affects antifouling performance is investigated. It is shown that the tadpole-shaped PHEAA-modified surfaces exhibit better antifouling performance than linear copolymer precursor-modified surfaces with identical molar masses and chemical compositions. This may be primarily attributed to the presence of cyclic PHEAA head chain segments in the tadpole-shaped PHEAA copolymer, and the absence of interchain entanglements can facilitate the formation of smoother and densely packed grafts, which result in better antifouling properties.
研究领域:
材料科学
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表面化学
·
生物医学工程
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DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2022
·
IF 24.319
Yumeng Gao,Wentao Wang,Mohsen Mohammadniaei,Ming Zhang,Jian Shen,Ninglin Zhou
Bacterial infection has become a global health issue. The misuse of antibiotics has been resulting in increased drug resistance and bioaccumulation. Therefore, developing a highly safe antibacterial agent, with high antibacterial performance is demanding. Inspired by the natural motors performing automated tasks in complicated living environments, we demonstrate tadpole-like nanoparticles (TNPs) with several functions, including high photothermal conversion efficiency, peroxidase-like catalytic activity, glutathione peroxidase-like activity, and catalase-like activity. TNPs produce hydroxyl radical (•OH) at an extremely low concentration of hydrogen peroxide of 0.006%, which can damage bacterial cell membranes, proteins, and DNA. Moreover, the glutathione peroxidase-like activity disrupts the anti-oxidative mechanism of bacteria and improves the permeability of the cell membranes, consequently enhancing the killing effect of ROS. In addition, TNPs possess tadpole-like asymmetry to overcome Brownian motion, demonstrating strong directional motion propelled by O 2 . The in vivo experiments indicate that TNPs could also shorten the inflammatory period and promote angiogenesis, making them a very promising antibacterial agent.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
微生物学
·
治疗学
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高影响因子
危险品化学品经营许可证（带存储）