Qi Huang,Yanmei Zhou,Qingyou Zhang,Enze Wang,Yinghao Min,Han Qiao,Junli Zhang,Tongsen Ma
Based on spirolactam ring-opening reaction, a chromogenic and “off–on” fluorescent sensor for determination of palladium (II) was developed in this paper. The novel sensor was easily synthesized from rhodamine B hydrazide and diacetyl monoxime named RBM. The structure of the sensor has been characterized by ESI-MS and NMR. EDTA-adding experiment indicated that the binding of chemosensor RBM and Pd 2+ was chemically reversible. The fluorescence intensity and absorbance of the sensor showed a good linearity with the concentration of Pd 2+ in the range of 0–15 μM and 0–17 μM, respectively, with detection limits of 0.015 μM and 0.200 μM. Moreover, it has been successfully applied to detect Pd 2+ in palladium-containing catalyst, natural water samples and imaging of Pd 2+ in living cells with satisfying results. The study indicated that this novel sensor could be an attractive approach to detect Pd 2+ in environmental systems and biological systems.
The dichromate as an industrial oxidant has a very extensive application. However, as one of the heavy metal elements, chromium has become a great threat to the organism and environment due to its carcinogenicity and mutageicity. So far, there is only a small number of detection and imaging methods for dichromate. In this work, a new fluorescent probe RhoCr for detecting dichromate based on in situ Cr 3+ -controlled opening of spirolactam-ring in a rhodamine dye after Cr 2 O 7 2− reduced to Cr 3+ by NaHSO 3 in solution and self-reduction in organism is reported, which displays a strong enhancement of fluorescence emission (λ em 582 nm), fast response (completed within 2 min) and excellent sensitivity (DL 1.18 nM). RhoCr successfully obtained fluorescence images of the intracellular Cr 2 O 7 2− . In addition, the effect have been exhibited in tap water and pool water, which will be crucial to the detection of dichromate in the environment.
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