Applied Sciences-Basel
·
2021
·Vol. 11
·期 10
·4578
·
IF 2.679
Xiaofeng He,Liling Xie,Xiaoshan Zhang,Fan Lin,Xiaobo Wen,Bo Teng
Aged swim bladders from the yellow drum (Protonibea diacanthus) are considered collagen-based functional food with extremely high market value. The structural integrity of collagen may be crucial for its biological functions. In the current study, swim bladders with 25-year-old sequences were collected and found to be basically composed of collagen. Then, thermogravimetry (TG), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and attenuated total reflectance–Fourier transform infrared spectroscopy (ATR–FTIR) were conducted to evaluate the integrity of the peptide chain and triple helix in the collagen. The structures of microfibers and fiber bundles were revealed with atomic force microscopy (AFM), scanning electrical microscopy (SEM), and optical spectroscopy. The collagens in the aged swim bladders were found to have similar thermal properties to those of fresh ones, but the relative content of the triple helixes was found to be negatively correlated with aging. The secondary structure of the remaining triple helix showed highly retained characteristics as in fresh swim bladders, and the microfibrils also showed a similar D-period to that of the fresh one. However, the fiber bundles displayed more compact and thick characteristics after years of storage. These results indicate that despite 25 years of aging, the collagen in the swim bladders was still partially retained with structures.Keywords:collagen;triple helix structure;swim bladder;aging
研究领域:
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xu Wanjun,Yu Qingqing,Zhang Yaqi,Fu Yu,Cheng Qiaoyu,Gao Ruichang,Bao Yulong
The purpose of this study was to investigate the effect of oxidation on the physicochemical properties of gelatin and gelatin gels. Porcine skin gelatin was oxidized with different concentrations of H 2 O 2 (0–30 mM). Upon oxidation of gelatin, a significant modification of amino acid residues including glycine, proline , hydroxyproline, and hydroxylysine occurred. Zeta-potential, ordered secondary structure and the fraction of triple-helix decreased, while particle size and surface hydrophobicity increased. Gels made from oxidized gelatin showed a looser network structure indicated by scanning electron microscope, and the gels had a weakened mechanical strength and water-holding as compared to non-oxidized gelatin gels. Based on these results, a mechanism of how oxidation affects the gelatin gel properties was proposed: Oxidation-induced increase of hydrophobicity and decrease of net charges promoted aggregation between gelatin molecules, thereby limiting the formation of triple-helix, which subsequently leads to a loose network structure and eventually a weakened gel strength and water-holding capacity.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Simin Feng,Di Luan,Ke Ning,Ping Shao,Peilong Sun
Brasenia schreberi ( B. schreberi ) is a rare and precious vegetable, which coat with a gelatinous mucilage. This study is aiming to investigate the relationship between structural characteristics and hypoglycemic activities of polysaccharides with different molecular weight ( Mw ) range. Three polysaccharides fractions, namely BSP-U100 (50–100 kDa), BSP-U50 (10–50 kDa) and BSP-U10 (<10 kDa), were isolated from B. schreberi mucilage using ultrafiltration method. Compared to other polysaccharide samples, only BSP-U100 had a triple helix structure and existed as the ordered structure in aqueous solution. Furthermore, BSP-U100 exhibited higher α-amylase (IC50 = 0.4414 mg/mL) and α-glucosidase (IC50 = 0.5993 mg/mL) inhibitory activity. A heteropolysaccharide BSP-1a, which average Mw was 83.98 kDa, was purified from BSP-U100 by DEAE Sepharose Fast Flow and Sephadex G-75 column. D-galactose (66.85%), d -glucose (2.05%) and D-mannose (1.65%) were three main monosaccharides of BSP-1a. Structural characterization of BSP-1a showed that BSP-1a might be a kind of L-D-pyranose type galactose and mainly composed of α -1 → 6-linked Galp, α -1 → 3-linked Manp and α -1 → 4-linked Glcp. This study provided a theoretical basis for the development and application of B. schreberi polysaccharides as a potential anti-diabetic supplement.
研究领域:
植物化学
·
药理学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2019
·Vol. 44
·期 1
·e13092
·
IF 1.358
Li-Li Gong,Fan-Ju Meng,Yu-Chen Hou,Yu Liu,Jia-Jia Xu,Wen-Na Zhang,Yan Chen
Two new polysaccharide fractions (TZP1-1 and TZP2-1) were obtained from the fruiting bodies of Thelephora ganbajun using DEAE-52 cellulose and Superdex 200 columns chromatography. The physiochemical characterization and biological activities of TZP1-1 and TZP2-1 were investigated. The relative molecular weight of TZP1-1 and TZP2-1 were 2.07 × 10 6 and 4,886 Da, respectively. TZP1-1 included mannose, rhamnose, galactose, and xylose (4:1:83.9:7.5), while TZP2-1 included mannose, glucose, galactose, and xylose (5.4:1:79.0:8.1). The Congo red experiment results confirmed that TZP2-1 had triple helix conformation. Furthermore, both TZP1-1 and TZP2-1 showed a certain cytotoxicity on HeLa and SH-SY5Y cells, while they exhibited a stronger inhibitory effect on HeLa than SH-SY5Y. Besides, the cytotoxicity of TZP1-1 was better than that of TZP2-1. Moreover, both of them exhibited a moderate inhibitory effect on α -amylase and α -glucosidase. These findings could promote the application of polysaccharides from T. ganbajun . Practical applications Thelephora ganbajun is an edible fungus widely distributed in Southwestern China. T. ganbajun polysaccharides as important active ingredients have not been reported. In this current study, two polysaccharides fractions (TZP1-1 and TZP2-1) were characterized, and their cytotoxicities and antidiabetic effect were also assayed. These findings could promote polysaccharides from T. ganbajun to be better application.
研究领域:
食品科学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2017
·
IF 1.753
Wenlu Pan,Yuming Zhou,Man He,Xiaohai Bu,Binbin Ding,Tingyuan Huang,Shuang Huang,Shiwei Li
Novel chiral N -propargylamide based on stigmasterol (M1, HC CCH 2 NHCOCH 2 CH 2 COO-R, R = stigmasteryl) and achiral N -propargylamide based on pivalic acid (M2) were prepared and (co)polymerized with rhodium zwitterions catalyst in CHCl 3 to afford helical polyacetylenes with moderate molecular weights (8997–26777) in good yields. The polymers were characterized by Fourier transform infrared spectroscopy (FT-IR), 1 H nuclear magnetic resonance ( 1 H NMR), gel permeation chromatography (GPC). Ultraviolet–visible spectroscopy (UV–Vis) and circular dichroism (CD) absorption spectra demonstrated that the as-prepared polymers could form helical structures with a preferential helicity. The formation of hydrogen bonds of poly(M1) in CHCl 3 was investigated according to the solution sate IR spectroscopic data . The single-handed helical conformation and polymerization degree of the new-typed poly( N -propargylamide)s could be controlled by the changing the feed ratio of chiral and achiral monomers . Furthermore, the infrared emissivity values (0.536–0.798) of copolymers were investigated at 8–14 μ m by the Infrared Emissometer.
研究领域:
高分子化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2023
·
IF 6.2
Qi Zhang,Xiaojing Li,Kui Huang,Yongjie Huang,Suwen Zhao,Shanshan Liu,Yang Li
Collagen hybridizing peptides (CHPs) are a powerful tool for targeting collagen damage in pathological tissues due to their ability to specifically form a hybrid collagen triple-helix with the denatured collagen chains. However, CHPs have a strong tendency to self-trimerize, requiring preheating or complicated chemical modifications to dissociate their homotrimers into monomers, which hinders their applications. To control the self-assembly of CHP monomers, we evaluated the effects of 22 cosolvents on the triple-helix structure: unlike typical globular proteins, the CHP homotrimers (as well as the hybrid CHP–collagen triple helix) cannot be destabilized by the hydrophobic alcohols and detergents (e.g., SDS) but can be effectively dissociated by the cosolvents that dominate hydrogen bonds (e.g., urea, guanidinium salts, and hexafluoroisopropanol). Our study provided a reference for the solvent effects on natural collagen and a simple effective solvent-switch method, enabling CHP utilization in automated histopathology staining and in vivo imaging and targeting of collagen damage.
研究领域:
生物化学
·
分子生物学
·
医学材料学
✓ 已验证
DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2015
·
IF 2.515
Chunyu Zhang,Yu Zhang,Hexin Zhang,Yanming Hu,Xuequan Zhang,Toshio Masuda
Acetylenic monomers containing salicylidene Schiff-base groups ( 1a and 1b ) as well as Schiff-base and hydroxy groups ( 1c ) were synthesized and polymerized with [(nbd)RhCl] 2 /Et 3 N catalyst to afford the corresponding polymers 2a – c with high molecular weights ( M n = 2.6–7.2 × 10 5 ) in high yields (75–97%). Polarimetric, circular dichroism (CD), and UV–vis spectroscopic analyses indicated that the polymers formed helical structures with a predominantly one-handed screw sense. The addition of metal ions to salicylidene Schiff-base-containing polymers 2a and 2b produced insoluble polymer/metal complexes through ionic cross-linking as a result of salicylaldimine–metal ion complexation. Polymers 2b and 2c underwent a helix–coil transition upon the addition of HSO 4 − , whereas these polymers did not exhibit responsiveness to other anions, such as F − , Cl − , and Br − .
研究领域:
高分子化学
·
材料科学
·
有机化学
✓ 已验证
产品关联
DOI
高影响因子
CHIRALITY
·
2017
·
IF 1.956
Yin Zhao,Lu Yin,Jingjing Liu,Hailing Chen,Wei Zhang
Conjugated homopolymer poly(9,9-bis(3-(( S )-2-methylbutylpropanoate))fluorene) (PSF) with chiral pendants was synthesized and characterized. Dissolution experiments show that PSF is well dissolved in racemic limonene at high temperature and begins aggregating upon sequential cooling treatment. The corresponding assemblies were transferred to quartz plate by the spin-coating method. Comparably, film casting from chloroform solution was also prepared. Upon annealing thermal treatments, these PSF films exhibited perfect mirror circular dichroism (CD) Cotton effects and dissymmetry ratios. Optical absorption spectroscopy (UV-vis), CD, and fluorescence spectroscopy results reveal that chiral side chains successfully induced M - and P -helical structures in aggregates and films, and this significant difference was ascribed to their differential supramolecular conformations.
研究领域:
高分子化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-11
·
IF 16.6
Peng Huawen,Yu Kaicheng,Liu Xufei,Li Jiapeng,Hu Xiangguo,Zhao Qiang
Current polyamide lithium extraction nanofiltration membranes are susceptible to chlorine degradation and/or low permeance, two problems that are hard to reconcile. Here we simultaneously circumvented these problems by designing a quaternized-spiro piperazine monomer and translating its beneficial properties into large-area membranes (1 × 2 m 2 ) via interfacial polymerization with trimesoyl chloride. The quaternary ammonium and spiral conformation of the monomer confer more positive charge and free volume to the membrane, leading to one of the highest permeance (~22 L m −2 h −1 bar −1 ) compared to the state-of-the-art Mg 2+ /Li + nanofiltration membranes. Meanwhile, membrane structures are chlorine resistant as the amine–acyl bonding contains no sensitive N-H group. Thus the high performance of membrane is stable versus 400-h immersion in sodium hypochlorite, while control membranes degraded readily. Molecular simulations show that the high permeance and chlorine resistance, which were reproducible at the membrane module level, arise from the spiral conformation and secondary amine structures of the monomer.
研究领域:
材料科学
·
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Hairui Huang,Hanyu Wu,Yizhou Zhang,Xunda Feng
Molecular-scale adsorption, catalysis, and separation demand nanoporous materials with high permeability, extensive surface areas, and pronounced nanoconfined effects. Fabricating polymeric particles with 3-D interwoven pores of ∼1 nm potentially addresses these needs. However, significant challenges remain in controlling their pore interconnectivity, uniformity, and achieving faceted particle shapes. Herein we present facile fabrication of polyhedral particles possessing interpenetrating 1 nm pores by suspension polymerization of double-gyroid (DG) liquid crystalline droplets. Mechanical stirring of the disordered phase at elevated temperatures, followed by undercooling, leads to the emulsification of DG droplets, as confirmed by synchrotron small-angle X-ray scattering (SAXS). UV-induced cross-linking of the DG droplets preserves the ordered network of 1 nm pores, as characterized by SAXS and microscopy. Intriguingly, due to the elasticity induced by the Ia3̅d periodicities, these particles adopt polyhedral shapes to avoid the elastic energy penalty associated with conventional sphericity. We demonstrate that these faceted particles, featuring 1 nm pores and efficient packing, enable rapid, size-exclusive adsorption and nanoconfined degradation of organic pollutants, driven by their 3-D permeability, high surface area, and enhanced nanoconfinement effects.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
BIOPOLYMERS
·
2012
·Vol. 97
·期 12
·988-997
·
IF 2.87
Qingye Liu,Xiaojuan Xu,Lina Zhang
We have firstly demonstrated the renaturation process of dissociated single chains of lentinan (s-LNT) and the variable conformations of the renatured LNT (r-LNT). The results from ultrasensitive differential scanning calorimetry and circular dichroism revealed that the variable structures including perfect triple helix, defective triple helix containing duplex segment, and single chains occurred in the renaturation of s-LNT, depending on the renaturation time, solvent composition, molecular weight, and the mode of renaturation. When water was added into s-LNT/dimethylsulfoxide (DMSO) to reach 95% (v/v), the classic low-temperature intra-triple-helical conformational transition at ∼10 ° C ( T 1 ) appeared within 4 h, indicative of a rapid reconstruction of triple helical structure. Besides, one newly endothermic peak at ∼43 ° C ( T 2 ) simultaneously occurred, which was first ascribed to the melting of duplex segment in the imperfect triplex. The duplex stretches disappeared when DMSO reached 50%, in which single chains coexisted with triplex. Moreover, the duplex segment disappeared by slowly dropping water into s-LNT/DMSO. This work suggested that the structure of r-LNT could be controllable, and provided important information for their successful development and application in polymer and life science. © 2012 Wiley Periodicals, Inc. Biopolymers 97:988–997, 2012.
研究领域:
生物化学
·
分子生物学
·
聚合物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xinxing Zhou,Bo Wu,Wei Wei,Kun Wang,Kerui Li,Chengyi Hou,Qinghong Zhang,Yaogang Li,Hongzhi Wang
The construction of high-density thermoelectric legs on flexible thermoelectric generators (F-TEG) to enhance thermal energy utilization has garnered widespread attention. However, designing an efficient spatial structure to increase the density of TE legs in F-TEG remains challenging. Inspired by the spiral configuration of nautilus shells, we have developed a novel belt-shaped flexible thermoelectric generator (BF-TEG) featuring a spiral multi-layered three-dimensional structure. This multi-layer structure not only increases the number of TE legs along the thermal gradient but also mitigates rapid heat transfer to the cold end, enabling high-performance output from the device. The BF-TEG exhibits an ultrahigh normalized Seebeck voltage of 0.518 mV/K cm −2 at a 40 K ΔT, which is double that of the state-of-the-art printed thin-film devices. Electricity can be generated directly without the need for a spacer in actual wear. Demonstrations of it powering electrochromic devices and LED bulbs show its potential as an energy supply device.
研究领域:
材料科学
·
能源技术
·
仿生学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）