ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Yi Jintao,Fu Nani,Zhong Wenhui,Liu Xia,Zhou Anqi,Li Xun
Amoxicillin (AMO), a penicillin antibiotic, and ciprofloxacin (CIP), a quinolone antibiotic, are widely used to combat bacterial infections and are frequently co-administered to enhance therapeutic efficacy. However, improper use of AMO and CIP may lead to antibiotic residues in the environment, posing serious threats to human health. The visual detection of AMO and CIP remains significant challenges. Herein, the Eu-DPA@CQDs was used for the dual-mode detection of AMO and CIP, which was synthesized by CQDs doped in europium-DPA metal-organic framework. In the colorimetric mode, the catalase-mimicking Eu-DPA@CQDs oxidized TMB to blue oxTMB. Upon addition of AMO, the oxTMB was reduced, causing color fading. Subsequent introduction of CIP restored nanozyme activity, regenerating the blue signal and enabling dual-analyte antibiotic detection. In the fluorescence mode, the Eu-DPA@CQDs probe exhibited dual emission at 440 nm and 613 nm. Upon AMO addition, the fluorescence at both wavelengths was quenched, accompanied by a color change from red to purple. In the presence of CIP, the emission at 440 nm increased and redshifted to 490 nm, while the signal at 613 nm was quenched, resulting in a multicolor transition from red to cyan. The Eu-DPA@CQDs sensor enabled rapid and dual-mode detection of AMO and CIP. The colorimetric method exhibited wide linear ranges of 0.005–25 μM for AMO and 0.007–4.25 μM for CIP, with detection limits of 1.67 nM and 3.75 nM, respectively. A visible color transition from dark blue to colorless was observed with increasing AMO concentration, while the addition of CIP reversed the signal back to dark blue. Similarly, the fluorescence detection achieved wide linear ranges from 0.005 to 30 μM for AMO and 0.005 to 15 μM for CIP, with detection limits as low as 4.74 nM and 3.11 nM, respectively, also within only 1 min. As the concentration of AMO increased, the color shifted from red to purple. In contrast, increasing CIP concentration induced a color transition from red to cyan. These color transitions were both clearly visible to the naked eye. The sensor demonstrated excellent selectivity and anti-interference capability, providing a reliable platform for accurate, sensitive, and quantitative determination of both antibiotics in real samples. Graphical abstract
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NEW JOURNAL OF CHEMISTRY
·
2026
·
IF 2.5
Weina Zhang,Hongji Li,Yuming Liu,Mengyang Xu,Dandan Wang
In this study, array structure SERS substrate was designed by using the polydimethylsiloxane (PDMS) as the support membrane and loaded Au@Ag@Au nanoparticles on its surface. By the three-phase self-assembly procedure, the Au@Ag@Au nanoparticles could smoothly transferred on the PDMS membrane and presented array structure, which could significantly promote the SERS sensitivity. Furthermore, molecularly imprinted polymers (MIPs) were coated on the surface of membrane to promote the selective detection property. In order to protect the chemical structure of MC-LR molecules, the Au@Ag@Au/PDMS molecularly imprinted membranes (AAAP-MIMs) were synthesized by the photo-initiated polymerization. It was demonstrated from the results that the AAAP-MIMs exhibited high SERS sensitivity, stability, and uniformity. This investigation presented a novel analysis procedure applied into the toxic microcystin selective detection.
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JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Qi He,Dandan Wang,Jing Shao,Yi Li,Ming Cheng,Lihui Dong,Yunhui Li,Jianwei Zhu,Hongji Li
A novel imprinting membrane (Ag/CuO/CNTs/Eggshell membrane -Molecularly Imprinted polymeric Membrances, ACCE-MIMs) based on surface enhanced Raman scattering (SERS) is successfully synthesized for the specific detection of spiramycin (SP). Firstly, CNTs and CuO are successively loaded on eggshell membrane (ESM) to increase the uniformity and sensitivity by virtue of the unique reticular structure of CNTs and excellent chemical enhancement of CuO. Through the abundant amino and carboxyl groups on ESM, Ag nanoparticles (Ag NPs) are anchored and growth on the surface of membrane by two in-situ reduction process and form a three-dimensional (3D) architecture. Eventually, the ACCE-MIMs are synthetized by silicon-based sol-gel method. With the help of imprinted polymers, the ACCE-MIMs present anti-interference performance during selective detection. The sensor presents a great liner relationship (R 2 = 0.9965) between SP concentration and the Raman peak intensity when the concentration of SP ranges between 1 × 10 −6 M ∼1 × 10 −12 M. The limit of detection concentration is 1 × 10 −12 M and the enhancement factor (EF) is 0.2956 × 10 7 . The ACCE-MIMs exhibits sensitive detection property and this procedure provides a promising future for detection process.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Hongji Li,Junfu Wang,Xiyue Wang,Haochen Yu,Linjing Ji,Tianyu Zhou,Chunbo Liu,Guangbo Che,Dandan Wang
In this test, the eggshell membrane (ESM) is selected as the support membrane for the biocompatibility and anchors CNTs on the surface to increase the mechanical properties. Then Ag NPs are decorated on CNTs-ESM substrate as SERS substrate by twice in-situ reduction. Finally, a layer of imprinted polymers is coated on the surface of the substrate to synthesize the imprinted membrane for selective detection of spiramycin. It is exhibited from the characteristic results that the CNTs significantly increase the mechanical properties and the detection sensitivity, simultaneously. When the concentration of SP changes between 10 -6 ∼ 10 -11 M, there is a linear relationship between SERS intensity and SP concentration. The detection limit is 10 -11 M, and the correlation coefficient R 2 is 0.9864. The SERS imprinted membrane can be applied into the detection of antibiotics in practical sample, which broadens the research field of antibiotics detection.
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PHARMAZIE
·
2023
·
IF 1.6
Yan Weifeng,Cui Zhe,Li Wenxin,An Xin,Cheng Xu,Wang Sihong,Jin Chunmei
New anti- Toxoplasma gondii agents are in demand due to the emergence of high toxicity. Ginseng polysaccharides and saponins can be used to treat the replication of Toxoplasma gondii in an attempt to determine whether the medicinal uses of ginseng are supported by pharmacological
effects. Anti- Toxoplasma gondii activities of ginseng polysaccharides and saponins were examined in vitro and in vivo. The findings are the survival time and rate of Toxoplasma gondii infected mice after the intake of the total polysaccharides and saponins increased
compared to untreated control mice. The survival rate of mice treated with ginseng saponins was the highest at 83.3%, the phenomenon of splenomegaly of mice was decreased especially ( p < 0.05) treated with ginseng polysaccharides. Accordingly, ginseng polysaccharides and saponins
have a potential application in anti-Toxoplasma gondii treatments.
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JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Yicheng Dou,Tingting Yan,Zhipeng Zhang,Qiya Sun,Lin Wang,Yongmei Li
In this study, peroxydisulfate (PDS) was activated by synthesized sulfur-doped g-C 3 N 4 (SCN) under visible-light irradiation and was adopted to enhance the removal of spiramycin, which is an important precursor of N-nitrosodimethylamine (NDMA). Specifically, 95.4% of spiramycin (≤10 mg/L) was removed in 60 min under the conditions of an initial value of pH of 7.0, an SCN dose of 1.0 g/L, and a PDS dose of 200 mg/L, and its degradation fitted well with the pseudo first-order kinetics. Electron paramagnetic resonance analysis and trapping experiments confirmed that ·O 2 − and h + were the main oxidizers for the degradation of spiramycin, and ·SO 4 − and ·OH also participated in the removal of spiramycin. The removal of spiramycin in the PDS/SCN visible-light catalytic system occurred through three different pathways: aldehyde oxidation , cleavage of C-O bond and demethylation. Notably, 61.4% of NDMA formation potential (FP) was reduced after the reaction. The SCN catalyst was stable and its catalytic performance was excellent in the PDS/SCN system, as the spiramycin removal efficiency decreased only slightly from 95.4% to 87.3% after being reused three times. Therefore, our study not only provides an alternative method for removing spiramycin but can also can significantly reduce NDMA FP.
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OPTICAL MATERIALS
·
2021
·
IF 3.08
Guoqi Sui,Xiuyun Yang,Hongji Li,Yunhui Li,Leijiao Li,Jing Shao,Yi Li,Junfu Wang,Yong Xue,Jinyue Zhang,Shuo Dong
A novel method for the detection of antibiotics is developed by combining surface enhanced Raman scattering (SERS) with molecularly imprinted polymers (MIPs) on membrane materials. In this test, eggshell membrane (ESM) is selected as support material, which shows the advantages of low price, easy preparation and more functional groups comparing with other common synthetic membranes. Firstly, Ag nanoparticles with different morphologies are deposited on the surface of ESM by in-situ reduction, and then imprinted polymer is coated by precipitation polymerization to promote high specific sensitivity. The results present that the sensitivity of SERS imprinted membrane is mainly affected by Ag morphology. Under the optimal conditions, it presents a good linear relationship between SERS intensity and spiramycin (SP) concentration, and the coefficient of determination (R 2 ) is 0.9981. It also exhibits the ability of selective detection and retrievability. It can be recycled at least 10 times. This method provides a cheap, stable and sensitive substrate, which broadens the research field of SERS detection of antibiotics.
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Foods
·
2021
·Vol. 10
·期 8
·1838
·
IF 4.35
Yu Wang,Jin-Yi Yang,Ying He,Lu Li,Jian-Xin Huang,Yuan-Xin Tian,Hong Wang,Zhen-Lin Xu,Yu-Dong Shen
Tylosin and tilmicosin (T&T) residues in livestock products have received extensive attention from consumers. Time-resolved fluorescence immunochromatographic assay (TRFICA), as a fast, efficient and sensitive immunoassay method, has played an increasingly important role in the food safety field. Therefore, herein a quantitative and visual TRFICA was established for simultaneously detecting T&T in milk in a group-screening manner. Under the optimal conditions, the standard curve range of developed TRFICA based on the T&T was 1.87~7.47 ng/mL, and the half-maximal inhibition concentrations (IC50) were 4.06 ng/mL and 3.74 ng/mL, respectively. The limits of detection (LOD) of the TRFICA method were from 1.72 ng/mL to 1.39 ng/mL, and the visual cut-off values were 31.25 ng/mL and 62.50 ng/mL for T&T in milk, respectively. Moreover, the stability experiments showed that the strips could be stored at 4 °C for more than 6 months, the total detection time was less than 13 min, and the cross-reactivities (CRs) with related compounds were less than 0.1%, which concluded that the developed TRFICA method could be used in real milk sample detection.Keywords:time-resolved fluorescence immunochromatographic assay;tylosin;tilmicosin;milk
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WATER RESEARCH
·
2021
·
IF 11.236
Lin Wang,Tingting Yan,Ruijie Tang,Qian Ping,Yongmei Li,Jie Wang
In this study, nanoscale zero-valent iron (nZVI) was added to motivate the functions of all the reactive oxidation species in peracetic acid (PAA) mixture under ultraviolet (UV) irradiation, and to enhance the removal of spiramycin, which is a typical precursor of N-nitrosodimethylamine (NDMA). Spiramycin (≤ 10 mg/L) could be completely removed within 20 min under the conditions of an initial pH of 4.0, a nZVI dose of 0.02 g/L and a PAA dose of 3.0 mg/L; additionally, 95.8% and 78.8% of PAA and H 2 O 2 were consumed during the process. Electron paramagnetic resonance analysis and quenching experiments confirmed that 52.4% and 44.8% of spiramycin removal was contributed by hydroxyl radical (•OH) and carbon-centered radicals (R-C•), respectively; and Fe 2+ released from nZVI played a critical role in radicals generation. Four degradation pathways of spiramycin were proposed and verified by the density of functional theory analysis. 65.2% of the NDMA formation potential (FP) was reduced after the reaction, and its residual was mainly contributed by the undegraded intermediate of dimethylamine. The results of multiple characterizations and continuous degradation experiments indicated that nZVI was stable in the system as the removal of spiramycin was hardly influenced even if reused three times. The nZVI/UV/PAA process is a promising advanced oxidation technology not only for the removal of refractory NDMA precursors (such as spiramycin) but also for significantly lowering the NDMA FP.
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RSC Advances
·
2019
·Vol. 9
·期 48
·28248-28252
·
IF 3.049
Yumin Leng,Fang Hu,Chunhua Ma,Chenxi Du,Linfeng Ma,Jiao Xu,Qiyuan Lin,Zhipei Sang,Zhiwen Lu
A simple, rapid, sensitive, selective and label-free method is presented for the colorimetric determination of lincomycin (Lin) by using HAuCl4 and NaOH. Upon the addition of Lin, the mixture of HAuCl4 and NaOH shows a color change from colorless to blue (or dark blue). The limit of colorimetric detection is as low as 1 μM, observed both in Milli-Q water and real samples. The selectivity of Lin detection is excellent compared with 9 other common antibiotics. On the basis of the “three-color” principle of Thomas Young, we extracted the red, green and blue (RGB) alterations of the sensor in the absence and presence of different concentrations of Lin. The color changes are quantitatively illustrated by the total Euclidean distances (EDs = [ΔR2 + ΔG2 + ΔB2]1/2). The good linear relationship between the EDs and Lin concentration is used for the quantitative assay of Lin. The developed method demonstrates great potential for the detection of Lin in environmental water and milk.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2018
·
IF 0.824
Chan-Yuan YAO,Jin-Yi YANG,Zhen-Lin XU,Hong WANG,Hong-Tao LEI,Yuan-Ming SUN,Yuan-Xin TIAN,Yu-Dong SHEN
A specific polyclonal antibody was prepared based on a new hapten with stable structure. Based on this, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established for determination of tylosin residues in food and environmental samples. The experimental conditions were optimized as follows: the coating antigen and antibody were respectively diluted by 6000 times and 2000 times, and the competitive reaction time was 40 min. Under the optimized conditions, the method showed a detection limit of 0.07 ng mL −1 , an IC 50 of 1.39 ng mL −1 and a linear range of 0.17–11.0 ng mL −1 . The recoveries of spiked raw milk and water samples were ranged from 78.4% to 105.6%, with the RSDs of less than 15%. Good correlation between icELISA and HPLC method was obtained for spiked samples ( R 2 = 0.97). This method was suitable for the determination of tylosin residues in milk and water samples.
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EXPERIMENTAL PARASITOLOGY
·
2016
·
IF 1.623
Xiaochuan Zhang,Lili Jin,Zhe Cui,Changhao Zhang,Xue Wu,Hyun Park,Hongmei Quan,Chunmei Jin
Toxoplasma gondii ( T . gondii ) is an important pathogen which can causes serious public health problems. Since the current therapeutic drugs for toxoplasmosis present serious host toxicity, research on effective and new substances of relatively low toxicity is urgently needed. This study was carried out to evaluate the anti-parasitic effect of oxymatrine (OM) and matrine (ME) against T . gondii in vitro and in vivo . In our study, the anti- T . gondii activities of ME and OM were evaluated in vitro using cell counting kit-8 assay, morphological observation and trypan blue exclusion assay. In vivo , mice were sacrificed four days post-infection and ascites were drawn out to determine the extent of tachyzoite proliferation. Viscera indexes and liver biochemical parameters, such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione (GSH) and malondialdehyde (MDA), were examined to evaluate the toxicity of compounds to mice. As a result, OM and ME showed anti- T . gondii activity but low selectivity toxicity to HeLa cells. Both compounds also significantly decreased the number of tachyzoites in peritoneal cavity and recovered the levels of ALT, AST, GSH and MDA in liver. Moreover, the mice treated with OM or ME achieved better results in viscera index and survival rate than that of spiramycin. These results suggest that OM and ME are likely the sources of new drugs for toxoplasmosis, and further studies will be necessary to compare the efficacy of drug combination, as well as identify its action of mechanism.
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