ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
·
IF 4.527
Wenfeng Wang,Qun Wan,Yixin Li,Wenjun Xu,Xiangyang Yu
The extensive application of pesticides in agricultural activities has raised increasing concerns on crop contamination by pesticide residues . Vegetables seem more susceptible to pesticide contamination given the high-intensive application of pesticides during their entire growth, while information about transfer and cell diffusion characteristics of pesticides in vegetables is currently insufficient. Here, we investigated the uptake, translocation and subcellular distribution behaviors of four commonly used pesticides in Chinese cabbage ( Brassica rapa var. chinensis ) under laboratory hydroponic conditions. Root uptake of pesticides followed the order of fenbuconazole > avermectin > thiamethoxam > spirotetramat. Thiamethoxam was more readily to be translocated from vegetable root to shoot, while spirotetramat, fenbuconazole and avermectin preferentially accumulated in vegetable root. Cell soluble components were the dominant storage compartment for thiamethoxam. The majority of spirotetramat, fenbuconazole and avermectin were partitioned into the cell walls. Hopefully, results of this study would extend the current knowledge of pesticide bioconcentration behavior in food-crops and assist in properly evaluating the threats of pesticide residues to human health via food chain .
研究领域:
农业科学
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环境科学
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植物生理学
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食品安全
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yaqi Jin,Zihui Jin,Di Sun,Yilin Peng,Qiyang Zhao,Yue He,Jing Li,Yaohai Zhang,Yongliang Cui
Spirodiclofen, a spirocyclic tetronic acid derivative, has excellent acaricidal effect and is used worldwide to control the majority of important mite species. For monitoring its residue in food and environmental samples, two haptens containing different spacer arms were synthesized, a monoclonal antibody (mAb 5A4) against spirodiclofen was prepared, and a heterologous indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established. The 50% inhibition concentration (IC 50 ) of ic-ELISA was 25.46 ng/mL, and the working range was 5.59–133.85 ng/mL. The ic-ELISA showed no cross-reactivity with structural analogs of spirodiclofen and other commonly-used acaricides. The average recoveries from Shiranui citrus samples and Yangtze River water were 85.62%-97.74% and 85.95%-99.30%, respectively. In the analysis of 12 citrus samples, the results of the ic-ELISA were quite similar to those of ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Hence, the new immunosorbent assay provides a substitute method for the qualitative and quantitative of spirodiclofen in food and environmental samples.
研究领域:
农业化学
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分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
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IF 4
Zhengyi Mao,Wen Zhao,Dongping Chen,Zhiyan Jiang,Qiang Liu,Jiayun Li,Yunxuan Wu,Nannan Lv,Jianting Fan
The pine-forest guardian Dastarcus helophoroides mainly rely on olfaction to locate its host accurately and interact socially. Odorant binding proteins of D. helophoroides play an important role in olfactory recognition and transporting odors to olfactory receptors to trigger signal transduction. However, some compounds affect olfactory recognition of D. helophoroides by binding to odorant binding proteins. In this study, DhelOBP4 and DhelOBP21, which are involved in the recognition of plant volatiles, were expressed to explore compounds that affect olfactory recognition. The affinity of 24 compounds with DhelOBP4 and DhelOBP21 was investigated by vitro fluorescence competitive binding assays. It was found that cyetpyrafen, chlorfenapyr and spirodiclofen had high affinity with DhelOBP4 and DhelOBP21. At the same time, we found that these three pesticides had a good evasive effect on the D. helophoroides and elicited EAG response in a dose-dependent manner. Besides, it was found that silencing DhelOBP4 and DhelOBP21 would affect D. helophoroides identifying these three pesticides. Through homology modeling and molecular docking, we identified key amino acid sites involved in the binding of DhelOBP4 and DhelOBP21 to these three pesticides, which might revealed their specific binding molecular interactions. This study is helpful to provide a valuable addition of OBP-pesticide interactions of D. helophoroides .
研究领域:
昆虫学
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农药科学
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分子生物学
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生物化学
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生态学
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神经生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Jiaxuan Fan,Jing Li,Wenfeng Zhou,Haixiang Gao,Runhua Lu
Acaricide residues (ARs) have high toxicity and pose a significant chronic dietary risk to humans. In this work, a low-cost and efficient selective detection method for ARs was developed. Meanwhile, to reduce the matrix effects of samples, a novel and fast pretreatment technology called magnetic solid phase extraction is used. After the optimization and validation of the method, the method’s limit of detection and quantitation were calculated as 0.5–0.9 and 1.7–3.1 μg L −1 , respectively. Additionally, the adsorption experiment between sorbents and ARs was thoroughly investigated in terms of adsorption behavior, mechanism, and selectivity. Finally, two environmental water samples were selected to verify the feasibility of the proposed method. The relative recovery ranged from 87 % to 116 % with a relative standard deviation ranging from 0.27 % to 9.41 %. These results demonstrated that the proposed method was low-cost and efficient and had the ability to selectively detect multiple ARs.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 13
·1713-1721
·
IF 3.1
Yumei Yan,Zhili Liu,Wenfeng Zhou,Haixiang Gao,Runhua Lu
Herein, three patterns for the detection of fenpyroximate based on the response signal of gold nanoparticles are described. The strong interaction between the guanidine group of arginine-modified gold nanoparticles and the ester group of fenpyroximate led to the aggregation of the nanoparticles and to a variation of ultraviolet-visible light spectrum and color of the solution. Sensors were constructed based on the correlation of the concentration of fenpyroximate with the absorbance ratio (A650/A525) and the R value was obtained by extracting the color of the test solution by using a smartphone to take a photo of the solution, which was then analyzed by colorimeter software. The absorbance ratio increased linearly in the range of 0.225–0.375 mg L−1 and the limit of detection was 0.215 mg L−1, while the R value declined linearly in the range of 0.20–0.40 mg L−1 and the limit of detection was 0.21 mg L−1. Further, the gold nanoparticles could cause a fluorescence quenching of fluorescent dyes, such as rhodamine B, and it was found that the fluorescence could be quenched and then restored after aggregation; therefore, a fluorescence method based on fluorescence “off–on” was constructed, and the fluorescence quenching was found to increase linearly in the range of 0.0–1.0 mg L−1 and the limit of detection was 0.013 mg L−1. These three patterns indicated highly selective and sensitive response signals for fenpyroximate, and all were applied to the detection of fenpyroximate in apple juice, pear juice, and environmental water samples, with the results showing that the three methods could be mutually verified, with the recoveries ranging from 94.15% to 110.65%.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）