FOOD CHEMISTRY
·
2021
·
IF 7.514
Jiaxiang Luo,Óscar Monroig,Qicun Zhou,Douglas R. Tocher,Ye Yuan,Tingting Zhu,Jingjing Lu,Dongying Song,Lefei Jiao,Min Jin
The quality of crustaceans’ flesh has direct impact on consumers’ purchase choices, with water environment and dietary nutrition being effective ways to regulate flesh quality. The aim of present study was to investigate the impacts of water salinity (low, 4 and medium, 23) and dietary lipid source (fish oil and soybean oil) on nutritional values, texture, taste and odor of flesh of mud crab. While water salinity had no significant influence on nutritional values of crab flesh, crabs fed soybean oil displayed significantly lower contents of amino acids and n-3 PUFAs in muscle. However, crabs reared at low salinity showed reduced flesh hardness, chewiness and gumminess likely related to altered myofiber structure, that impacted muscle texture. Furthermore, low salinity and dietary soybean oil weakened umami taste and aroma characteristics of crab flesh associated with decreased contents of free amino acids, flavor nucleotides, inorganic ions and odor active compounds in flesh.
研究领域:
水产养殖
·
营养学
·
海洋生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Oceanology and Limnology
·
2023
·Vol. 41
·期 5
·1918-1939
·
IF 1.6
Guo Liwen,Zhao Yaqi,Guo Huarong
Crab cell line, especially continuous crab cell line, can provide us a useful tool for studies on the virology, immunology, and molecular biology of crabs. However, no continuous crab cell line has been available due to the lacking of suitable medium and the occurrence of mitosis-arrest. In this study, long-term in vitro culture conditions for both two- (2D) and three-dimensions (3D) were successfully developed for the circulating hemocytes of swimming crab Portunus trituberculatus , designated as PTH cells. In 2D culture, a novel crab basic medium in osmolarity of 990–1 100 mOsm/kg was optimized for the first time, which is different from Leibovitz’s L-15 medium in mainly the components of amino acids, containing double strengths of the contents of free amino acid mixture in the crab serum. Then an optimal crab growth medium was developed by supplementing 5% fetal bovine serum, 50-g/L yeast extract powder, 20-µg/L basic fibroblast growth factor and epidermal growth factor into the optimal crab basic medium, and found that it could support a long-term survival of PTH cells in a healthy monolayer up to 347 days and partially break through the mitosis-arrest of crab cells evidenced by the obvious increase of proliferating potential detected in the 10-d primarily cultured PTH cells. These 2D cultured PTH cells could be successfully sub-cultured for 11 times by physical flushing method and well cryopreserved in liquid nitrogen. In 3D culture, using the same crab growth medium, the PTH cell aggregates could be easily formed and healthily maintained on the surface of solidified Matrigel or in the ultra-low-attachment plate with a survival rate of 50%–60% on Day 103. This work largely improved the primary culture and subculture of crab cells and will facilitate the establishment of continuous crab cell line.
研究领域:
海洋生物学
·
细胞生物学
·
免疫学
·
病毒学
·
分子生物学
✓ 已验证
DOI
高影响因子
AQUACULTURE
·
2016
·
IF 1.893
Hong-Yue Tan,Guang-Ming Shao,Peng-Fei Kang,Yu-Feng Wang
The red swamp crayfish Procambarus clarkii is an important freshwater economic crayfish in Chinese inland due to its high market value and consumer demand. The sexual precocity in this species has received considerable attention in recent years because more and more individuals matured at small sizes, which devalues the commercial production. In this study, by the full-length normalization subtractive hybridization (FNSH) method we identified 16 differently expressed sequences between precocious and normal grown P . clarkii ovaries. Of the down-regulated sequences in precocious ovaries, one encodes a peptide which shared the highest identity to C1q-like family and contained a typical C1q domain at the C-terminal, thus it was named PcC1q-like . QRT-PCR analysis showed that PcC1q-like was transcribed specifically in ovary, with higher expression level in vitellogenic stages. In situ hybridization exhibited specific expression of PcC1q-like in follicular cells around the vitellogenic oocytes . Knockdown of PcC1q-like by injection of double strand RNA (dsRNA) resulted in disruption of follicular cells, decreased transcripts of vitellogenin ( Vg ) and vitellogenin receptor ( VgR ), and reduced progesterone content. These results suggest that PcC1q-like may promote ovarian development by stimulating the synthesis and transportation of vitellogenin into oocytes. These data provide insights into the molecular regulatory mechanisms of sexual precocity and ovarian development in crustaceans. Statement of relevance The red swamp crayfish Procambarus clarkii is one of the most important freshwater aquaculture species in Chinese inland. We screened 16 sequences that are potentially involved in sexual precocity of P. clarkii . One candidate was demonstrated to be a novel ovary-specific C1q-like gene of P. clarkii , thus named PcC1q-like . Knockdown of PcC1q-like resulted in disruption of follicular cells, decreased transcripts of Vg and VgR , and reduced progesterone, suggesting its essential role in vitellogenic ovarian development of P. clarkii .
研究领域:
水产养殖
·
分子生物学
·
生殖生物学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Ye Yuan,Xuexi Wang,Min Jin,Lefei Jiao,Peng Sun,Mónica B. Betancor,Douglas R. Tocher,Qicun Zhou
Lipid sources as alternatives to fish oil could alter the nutritional value and flavor quality of crab meat affecting consumer preferences. Herein, an 8-week nutritional trial was designed to investigate the effects of dietary lipid sources including fish oil (FO), krill oil (KO), palm oil, rapeseed oil, soybean oil and linseed oil on profiles of amino acids, fatty acids and volatiles in muscle of swimming crab ( Portunus trituberculatus ). Volatiles of crab muscle were characterized by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry. Results revealed that crabs fed FO and KO had significantly higher levels of protein, indispensable amino acids, eicosapentaenoic acid and docosahexaenoic acid in muscle. Principal component analysis and hierarchical cluster analysis demonstrated that muscle volatiles of crabs fed different dietary oils exhibited significant variations. Dietary FO and KO significantly increased the relative levels of 3-methylbutanal, heptanal, benzaldehyde and nonanal in muscle, which may produce more pleasant flavors.
研究领域:
水产养殖
·
营养学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DRUG METABOLISM AND DISPOSITION
·
2014
·Vol. 42
·期 5
·839-843
·
IF 3.334
Ke Li,Sylvia Zhao,Lingling Zhang,Xiaoyun Wu,Pan Shu,Yang Wang,Hao Feng,Zheming Gu,Helen Han Hsu
It has been proposed that in humans 4β-hydroxycholesterol is formed mainly by CYP3A-catalyzed metabolism of cholesterol and thus may serve as an endogenous marker for CYP3A activity. The cynomolgus monkey is widely used as one of the nonrodent preclinical safety species in pharmaceutical research. In the current study, the potential application of 4β-hydroxycholesterol as an endogenous biomarker of CYP3A in response to drug treatment was evaluated in cynomolgus monkeys. Following multiple oral administration of rifampicin (a known CYP3A inducer) at 15 mg/kg/d in cynomolgus monkeys, the mean serum 4β-hydroxycholesterol levels increased 4-fold from the baseline of 55.3 ± 21.7 to 221 ± 53.4 ng/ml. The mean concentration ratios of 4β-hydroxycholesterol to cholesterol increased 5-fold. The data suggest that 4β-hydroxycholesterol formation from cholesterol metabolism was induced by rifampicin treatment in monkeys. This observation correlated with the metabolism of midazolam (a probe substrate of CYP3A activity) monitored in the same study. The serum exposure (area under the curve) of midazolam was markedly decreased by ∼95%, confirming the induction of CYP3A catalytic activity by rifampicin treatment in monkeys. The formation of 4β-hydroxycholesterol from cholesterol was specifically mediated by recombinant cynomolgus CYP3A8 and CYP3A5. The Km values of CYP3A8 and CYP3A5 for 4β-hydroxycholesterol formation from cholesterol were 204 and 104 μM, respectively, and Vmax values were 0.600 and 0.310 pg/pmol/min, respectively. The results suggest that 4β-hydroxycholesterol can be used as an endogenous biomarker to identify strong CYP3A inducers in cynomolgus monkeys, which may help to evaluate drug-drug interaction potential of drug candidates in preclinical settings.
研究领域:
药理学
·
毒理学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE RESEARCH
·
2018
·Vol. 49
·期 10
·3434-3441
·
IF 1.475
Puqiang Zheng,Jiteng Wang,Tao Han,Min Yang,Xinyu Li,Chunlin Wang
A 56-day feeding trial was conducted to investigate the effect of dietary cholesterol (CHOL) levels on growth performance, body composition and gene expression of juvenile mud crab ( Scylla paramamosain ). Four isonitrogenous and isoenergetic diets were formulated with 0.4%, 0.8%, 1.2% and 1.6% CHOL supplemented, and the final dietary CHOL concentrations were 0.72%, 1.11%, 1.49% and 1.83% respectively. Each dietary treatment was performed with three replicates (28 crabs per replicate, initial body weight 0.04 g). At the termination of the experiment, although the survival had no statistic difference in all treatments, the mud crabs fed the lowest CHOL diet had the lowest survival rate. The weight gain (WG) of mud crab significantly increased with dietary CHOL level up to 1.11% and then significantly decreased with dietary CHOL level further increased. The total-cholesterol (T-CHOL) content in whole body had an increasing trend with the dietary CHOL level increased. Besides, dietary CHOL supplement generally increased the hepatic superoxide dismutase (SOD) activity, and the mud crabs fed diets CHOL1.11 and CHOL1.49 showed significantly higher value than those fed other diets. The hepatic aspartate aminotransferase (AST) activity decreased slightly with dietary CHOL level up to 1.11% and then significantly increased with CHOL level further increased. The mRNA expression of ecdysone receptor (EcR) gene in the eyestalk obviously increased with dietary CHOL level up to 1.11% and then significantly decreased with dietary CHOL further increased. These results suggested that about 1.11% dietary CHOL seem fulfil to maintain good growth performance and healthy condition for juvenile S. paramamosain .
研究领域:
水产养殖学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2024
·
IF 4.7
Ma Jinjing,Yu Yu,Chu Deyu,Zhu Shaomin,Liu Qishun,Yin Heng
Chitin is the second most abundant biopolymer in the world after cellulose and commonly found in crustaceans. It is also the most abundant natural nitrogen-containing polysaccharide. The challenge in separation of chitin directly from snow crab ( Chionoecetes opilio ) shells is that the solvent used must be versatile in removing calcium carbonate and protein while also being efficient, environmentally friendly, and cost-effective. In this study, chitin was extracted from snow crab shells using a DES consisting of TEBAC and lactic acid. DES successfully removed minerals and proteins, with a deproteinization rate of 95.51% and a decalcification rate of 96.41%, while the purity of chitin was 91.15% and the yield was 21.31%. Furthermore, the extracted chitin has large pores on its surface, making it an ideal matrix for biomedical materials. This study provides an environmentally friendly and cost-effective solution for the utilization of crustacean waste using multifunctional DES.
研究领域:
生物化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
Xuebin Lu,Zhengya Gong,Zhihao Yu,Jian Xiong,Gaixiu Yang,Mengyan Guo,Yuxin Zhang,Yiming Li
Supercapacitors as one of the new energy storage devices have attracted much attention from researchers. The design and preparation of new electrode materials with high conductivity and energy density has become a frontier and key issue in the research of supercapacitor performance improvement. In addition, flow capacitor deionization (FCDI) relying on the working principle of supercapacitors is a promising technology for desalination and has received crucial attention. NiCo 2 S 4 (NCS) is a promising pseudocapacitive material with high electrical conductivity and ideal stability. In this study, sea urchin-like NiCo 2 S 4 electrode materials (CC@NCS) grown in situ on crab shell carbon (CC) were prepared by a simple molten salt-assisted hydrothermal two-step method. The composite successfully combined the properties of CC and NiCo 2 S 4 . The prepared material exhibited excellent electrochemical properties and energy storage performance. In the three-electrode system, the specific capacitance is up to 692.4 F/g at a current density of 0.5 A/g. Asymmetric supercapacitors assembled with CC as the negative electrode, CC@NCS as the positive electrode, and 3 M KOH as the electrolyte obtained high specific capacitance (0.5 A/g, 163.6 F/g) and high energy density (305.16 W/kg, 8.18 Wh/kg). The device also showed excellent cycling performance in the long cycle test (88.65 % retention after 5000 cycles). In addition, the FCDI device with this material as the flow electrode achieved a final conductivity of 15.16 μS/cm by electrosorption of a 1000 mg/L NaCl solution at a constant pressure of 1.2 V for 60 min along with an impressive average salt removal rate (0.0621 mg/cm 2 ·min −1 ) and charge efficiency value (90.79 %), and a low energy consumption value (0.02 kWh/mol). What's more, it also exhibited excellent desalination properties. This indicates that the composite is a promising active material for energy storage and desalination.
研究领域:
材料科学
·
能源存储
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
Yiming Li,Zhihao Yu,Mengyan Guo,Ming Zhang,Jian Xiong,Yina Qiao,Rui Zhang,Xuebin Lu
Carbon-based materials have become the most commonly used electrode materials for supercapacitors due to their simple production and low preparation cost. The preparation of hierarchical porous carbon materials with excellent properties using biomass materials is a very valuable study. Therefore, we used crab shells as carbon precursors to prepare crab shell-based carbon materials by using the self-templating method (CaCO 3 ) combined with low-temperature eutectic molten salt. When the composition of the eutectic salt was CaCl 2 /KCl (molar ratio of 43:57) and the mass ratio of crab shells to eutectic salt was 1:5, the prepared crab-shell-based carbon material (CC-CaK-5) exhibited excellent physical and electrochemical properties with high specific surface area (1193.7 m 2 g −1 ), rich heteroatom doping (O (7.83 %) and N (6.71 %)), and high specific capacitance (290 Fg −1 at 0.5 Ag −1 ).The CC-CaK-5//CC-CaK-5 devices assembled with CC-CaK-5 as the electrode material exhibited excellent electrochemical performance. At a current density of 1 Ag −1 , the capacitance value of the device remained at 93.11 % of the initial capacitance value after 5000 cycles. The energy density reached 6.95 Whkg −1 at a power density of 302.09 Wkg −1 and remained at 4.71 Whkg −1 at a power density of 13,037.54 Wkg −1 .
研究领域:
材料科学
·
能源存储
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Food Science & Technology
·
2025
·
IF 2.8
Yiruo Wang,Hongyu Gan,Qifeng Wei,Shanshan Wang,Xiulian Ren
Deep eutectic solvents (DESs) are considered green due to their low cost, nontoxicity, low flammability, and biodegradability. Although the DESs-based method has been applied for chitin extraction, the efficiency of protein leaching from crustacean shells and the cyclic performance of DESs have not been thoroughly evaluated. In this study, chitin was extracted from crab shells using the DES consisting of choline chloride and lactic acid, achieving a purity of up to 95.14% by optimizing the reaction conditions and minimizing energy consumption. The kinetics of protein leaching were investigated, revealing that protein first diffused across the surface of the shells and subsequently entered the DES through pore diffusion. Mechanistic analysis showed that lactic acid effectively removed calcium carbonate from the crab shells, while protein leaching primarily relied on hydrogen bonding interactions. During the extraction process, lactic acid was consumed to remove calcium carbonate, necessitating the addition of a certain amount of lactic acid after each cycle to maintain the optimal choline chloride/lactic acid ratio. The results demonstrated that the purity and yield of chitin extracted by the DES remained relatively stable after five cycles. This cycling method of the DES supplemented with lactic acid is expected to achieve green and sustainable extraction of high-purity chitin from crab shells.
研究领域:
生物化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2024
·
IF 3.9
Mengting Huang,Yuan Ma,Qianru Fan,Shunli Che,Jun Zhang,Shuquan Ding,Shuren Zhu,Xilei Li
Nanopolystyrene (NP) and phoxim (PHO) are pervasive environmental contaminants that pose a significant threat to the health of aquatic organisms, prompting widespread concern among researchers and the public alike. The hepatopancreas play important roles in the Chinese mitten crab ( Eriocheir sinensis ), such as digestion, absorption and detoxification. This study assessed the hepatopancreatic toxicity caused by the exposure of Eriocheir sinensis to environmentally relevant concentrations of NP and/or PHO. After a 21-day exposure period, NP (1.0 × 10 10 particles/L) and PHO (24 μg/L) exposure resulted in reduced number of blister-like, resorptive, and fibrillar cells and an elevation in lipid droplets within the hepatopancreas compared to the control group. Furthermore, trypsin and lipase activity decreased, amylase activity increased, and a significantly decrease in the expression of digestion-related genes, including CHT , CarL , and CarB , suggested impairment in both digestive and metabolic functions. The marked upregulation of key genes, including PPARγ , GYK , PEPCK , and SCD , as well as key metabolites such as 4-methylzymosterol-carboxylate, zymosterone, lathosterol, 7-dehydro-desmosterol, vitamin D2, 24-methylene-cycloartanol, 5-dehydroepisterol, and sitosterol in the lipid metabolic pathway, showed that the peroxisome proliferator-activated receptor (PPAR) and steroid biosynthesis signaling pathways were highly affected by exposure to NP and/or PHO. These findings indicated that exposure to NP and/or PHO might adversely affect the hepatopancreatic physiological homeostasis in E. sinensis by causing tissue damage and interfering with digestive and metabolic functions. Our results provide ecotoxicological insights into the effects of nanopolystyrene and/or phoxim exposure on the digestive function of Eriocheir sinensis .
研究领域:
环境科学
·
生态毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2024
·
IF 3
Xiaorong Huang,Ru Tan,Zhi Geng,Tao Zhang,Guangpeng Feng,Gang Yang,Feng Zhao,Ping Zhuang
A 60-day feeding trial was conducted to evaluate the impact of dietary Antarctic krill meal on the reproductive performance and embryo quality of the Chinese mitten crab, Eriocheir sinensis. Three diets were formulated, incorporating varying levels of Antarctic krill meal at 0% (Diet K0), 10% (Diet K10), and 20% (Diet K20), with a control group fed razor clam Sinonovacula constricta. Each diet was randomly assigned to three replicate tanks, each stocked with 5 males and 10 females. Male and female weights were 145.38 ± 8.01 and 102.57 ± 9.73 g, respectively. The results revealed no significant differences in weight gain rate, specific growth rate, and survival rate. However, the hepatopancreatic weight and hepatopancreas index of female crabs in each group decreased, while gonadal weight and gonadosomatic index increased significantly after 60 days, with Diet K20 showing the highest values. Egg production and fecundity of female crabs reached their peak in Diet K20, with no significant differences in reproductive indices among all groups. The phospholipid content in Diet K20 was significantly higher than in the other groups (). Cholesterol contents in Diet K0 and the control group were significantly higher than in Diet K10 and K20 (). No significant differences were observed in egg diameter, egg weight, moisture, crude protein, and crude fat between the groups. The content of C20 : 2 and C20 : 4n6 was highest in Diet K0, with a significant difference compared to Diet K10 (). However, no significant differences were found in the total content of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids among all groups. Based on the research findings, it is recommended that the optimal level of Antarctic krill meal in diets is 20%.
研究领域:
水产养殖学
·
动物营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）