INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2019
·
IF 1.398
Yufeng Sun,Jiameng Liu,Yatao Huang,Minmin Li,Jia Lu,Nuo Jin,Yan He,Bei Fan
Fresh kidney beans (Phaseolus vulgaris L.) are common green vegetable that are widely cultured and consumed worldwide. The benefits of fresh kidney beans are highly substantial, but at the same time, toxicity caused by phytohemagglutinin (PHA) has raised concerns. PHA from fresh kidney beans accounted for 40% of poisoning events induced by poisonous plants in China from 2004 to 2013. However, an understanding of PHA levels in fresh kidney beans remains elusive. An analysis method for PHA based on agglutination of PHA to erythrocyte cell was established. We investigated the effect of cultivar, bean part, maturity, and cooking method on PHA content in fresh kidney beans. We demonstrated significant differences in the distribution of PHA among various predominant, fresh Chinese kidney bean cultivars and parts. One major Chinese strain, Zihuayoudou, was found to contain the highest amount of PHA, which was abundant in fresh kidney bean seeds. Moreover, PHA concentrations were found to decrease with the maturity of the fresh product. Further, cooking methods had a significant effect on PHA levels in fresh kidney beans. PHA could be effectively removed when stir-frying for more than 18 min, or braising for more than 10 min. These findings provide insight into the PHA content of fresh kidney beans in China and will be helpful to guide food safety measures.
研究领域:
食品科学
·
毒理学
·
植物生物学
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DOI
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2017
·
IF 4.581
Dongguo Lin,Yinzhu Luo,Guang Yang,Fangfang Li,Xiangkun Xie,Daiwei Chen,Lifang He,Jingyu Wang,Chunfeng Ye,Shengsheng Lu,Lin Lv,Shuwen Liu,Jian He
Influenza A viruses (IAVs) induce acute respiratory disease and cause significant morbidity and mortality throughout the world. With the emergence of drug-resistant viral strains, new and effective anti-IAV drugs with different modes of action are urgently needed. In this study, by conjugating cholesterol to the N-terminus of the short peptide KKWK, a lipopeptide named S-KKWK was created. The anti-IAV test indicated that S-KKWK and its derivatives displayed potent antiviral activities against a broad variety of influenza A viral strains including oseltamivir-resistant strains and clinically relevant isolates with IC 50 values ranging from 0.7 to 3.0 µM. An extensive mechanistic study showed that these peptides functioned as viral “entry blockers” by inhibiting the conformational rearrangements of HA2 subunit, thereby interrupting the fusion of virus-host cell membranes. Significantly, a computer-aided docking simulation and protein sequence alignment identified conserved residues in the stem region of HA2 as the possible binding site of S-KKWK, which may be employed as a potential drug target for designing anti-IAVs with a broad-spectrum of activity. By targeting this region, a potent anti-IAV agent was subsequently created. In addition, the anti-IAV activity of S-KKWK was assessed by experiments with influenza A virus-infected mice, in which S-KKWK reduced the mortality of infected animals and extended survival time significantly. Overall, in addition to providing a strategy for designing broad-spectrum anti-IAV agents, these results indicate that S-KKWK and its derivatives are prospective candidates for potent antivirals.
研究领域:
病毒学
·
药物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Guo Liu,Mingjuan Liao,Baoyan Guo,Qixin Kan,Shuangde Zhou,Konglong Feng,Wanhuang Lin,Yahui Huang,Jianyin Miao,Yong Cao
Castor ( Ricinus communis L.) meal, a by-product of castor oil production, is a high-quality protein source. Unfortunately, the toxic substance limits its application. Herein, a novel continuous phase transition extraction (CPTE) technology at pilot scale was explored for castor meal detoxification. The detoxification conditions were optimized using response surface methodology (RSM) as: treatment time of 3 h, extraction pressure of 0.16 MPa and temperature of 84.8°C. And the removal rate of allergens, hemagglutinin, and ricin reached 79.2 %, 99.8 % and 99.0 %, respectively. The protein content of castor meal increased from 38.4% to 39.0%. The nontoxicity nature of the treated caster meal was verified via an acute toxicity tests where the mean lethal dose was >5000 mg/kg body weight. Overall, CPTE offers a novel and efficient tool for castor meal detoxification, with the superiority of non-nutrient loss, it improved the application value of castor meal and reduced the risk of environmental pollution.
研究领域:
食品科学
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植物学
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DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 29
·3641-3647
·
IF 2.378
Chanjuan Liu,Chao Li,Guoyun Li,Guangli Yu
The specific interaction of carbohydrates and hemagglutinin (HA) on influenza A virus (IAV) is of great interest for carbohydrate-based anti-IAV drug discovery. Herein, a carbohydrate microarray-based method was developed for the determination of the specific interaction between carbohydrates and HA. Four marine acidic saccharides from algin (L-guluronic acid, GulA; L-diguluronic acid, GulA2; L-triguluronic acid, GulA3; and L-tetraguluronic acid, GulA4) were immobilized on poly(2-hydroxyethyl methacrylate)-cyanuric chloride-modified substrates, recently reported by us, to form a microarray. GulA, GulA2, GulA3, and GulA4 all showed specific binding with HA from influenza A H1N1 subtype and exhibited increased binding affinity. Molecular docking was adopted to simulate the complexes formed by investigated saccharides and HA to gain better insight into their binding behaviors. The reliability of the carbohydrate microarray-based assay was also verified by cytopathic effect inhibition assay and surface plasmon resonance analysis. By adopting this microarray-based method, the consumption of saccharides could be reduced to the pmol level and the limit of detection of HA could be reduced to 8.4 nM without any further signal amplification. This carbohydrate microarray-based assay is thus an ideal tool for studying specific interactions of carbohydrates and HA or other proteins, and will provide a promising platform for carbohydrate-based drug discovery and development.
研究领域:
病毒学
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生物化学
·
分子生物学
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药学
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产品关联
DOI
高影响因子
Cell Reports Physical Science
·
2023
·
IF 8.9
Liangliang Wang,Li Yang,Feng Zhang,Xuejie Liu,Qian Xie,Qingyun Liu,Lifang Yuan,Tianyi Zhao,Sidian Xie,Qiuyi Xu,Wudi Zhou,Lin Mei,Huacheng Yan,Xiaowei Zeng,Yuelong Shu
Summary Seasonal influenza A viruses continue to pose a public health threat, and current vaccines are not sufficiently effective because of virus mutation. There is an urgent need to develop a broad-protection influenza A vaccine. Our team previously designed potential universal hemagglutinin (HA) sequences against seasonal influenza A H1N1 and H3N2 (mH1 and mH3, respectively) through a mosaic strategy. In this study, we construct DNA vaccines by linking the antigens mH1 and mH3 via internal ribosome entry sites and then wrap the DNA with the deoxycholic acid-modified polyetherimide to form DNA nanovaccines. A microneedle is used to deliver DNA nanovaccines, and the data show that better cellular and cross-reactivity and protective immunity are induced compared with the intramuscular injection method. These results suggest that microneedle-based delivery of DNA nanovaccines could be a promising platform for development of a broad-protection influenza vaccine.
研究领域:
病毒学
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免疫学
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药学
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生物技术
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Xiaoling Pan,Pai Liu,Xinghui Wu,Yahui Zhang,Zhengxu Cai,Jianbing Shi,Junge Zhi,Zi Li,Dayan Wang,Bin Tong,Yuping Dong
Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited “turn-on” fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.
研究领域:
分析化学
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病毒学
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分子生物学
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药物设计
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产品关联
DOI
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2023
·
IF 5.8
Runhong Zhou,Jianan Hu,Jingnan Qiu,Shengsheng Lu,Haixing Lin,Ruifeng Huang,Shaofen Zhou,Guoqing Huang,Jian He
Influenza A virus (IAV) is one of the leading causes of respiratory illness and continues to cause pandemics around the world. Against this backdrop, drug resistance poses a challenge to existing antiviral drugs, and hence, there is an urgent need for developing new antiviral drugs. In this study, we obtained a phenolic compound SG -7, a derivative of natural compound 2-hydroxymethyl-1,4-hydroquinone, which exhibits inhibitory activity toward a panel of influenza viruses and has low cellular toxicity. Mechanistic studies have shown that SG -7 exerts its anti-IAV properties by acting on the virus itself and modulating host signaling pathways. Namely, SG -7 targets the HA2 subunit of hemagglutinin (HA) to block the fusion of viral-cellular membranes and inhibits IAV-induced oxidative stress and overexpression of pro-inflammatory factors by activating the Nrf2/HO-1 pathway and reducing NF-κB activation. In addition, SG -7 can enhance type I IFN antiviral response by inducing Nrf2 expression. Importantly, SG-7 showed the ability to inhibit viral replication in the lungs of IAV-infected mice and reduce their mortality. Therefore, SG-7 may be a promising lead compound for anti-influenza drug development .
研究领域:
药理学
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病毒学
·
分子生物学
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免疫学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Zhen Wan,Haijiang Gong,Qi Liu,Qingyu Wang,Ping'an Ma,Fei He,Qingqing Wang,He Ding,Shili Gai,Piaoping Yang
Rapid and specific detection of influenza A viruses is critical for pandemic response. However, traditional lateral flow immunoassays (LFIAs) face challenges in sensitivity, signal reliability, and cross-reactivity. Herein, a dual-aptamer-functionalized lateral flow platform is designed, integrating upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs) to mitigate these bottlenecks through a synergistic mechanism: near-infrared-excited luminescence resonance energy transfer (LRET) for fluorescence modulation, dual-aptamer domain recognition for specific binding, and fluorescence-colorimetric dual readouts for orthogonal validation. Specifically, LRET serves as the core signal transduction mechanism, with aptamer1-conjugated UCNPs as donors and aptamer2-conjugated AuNPs as acceptors, regulated by target-induced proximity. Dual-aptamers recognizing distinct hemagglutinin (HA) epitopes enhance specificity, minimizing cross-reactivity with non-target subtypes (H5N1, H6N1, etc.) while ensuring high-affinity H1N1 HA binding. Such synergy enables significant signal amplification, achieving a fluorescent limit of detection (LOD) of 2.814 ng mL −1 (2.84–5.33-fold better than traditional AuNP-LFIAs) with a linear range of 4–10 ng mL −1 . Importantly, the dual-mode design integrates upconversion fluorescence and AuNPs colorimetry, mitigating variability, ensuring precision, and enabling 20-minute qualitative detection (up to 100 ng mL −1 ) for point-of-care testing (POCT). Thus, this work presents a scalable POCT paradigm via apt-LRET integration, leveraging dual-aptamer recognition and LRET-mediated amplification for on-site viral diagnostics with pandemic-response timeliness.
研究领域:
病毒学
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生物传感器
·
即时检测
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纳米生物技术
·
诊断成像
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 23
·4130-4137
·
IF 4.872
Chunyu Zhao,Qiang Shi,Jianwen Hou,Zhirong Xin,Jing Jin,Chunming Li,Shing-Chung Wong,Jinghua Yin
A newly glycopolymer-patterned surface for capturing red blood cells (RBCs) is demonstrated. Our strategy is based on the surface-initiated photopolymerization of 2-acryl-amido-2-methylpropane sulfonic acid (AMPS) on a thermoplastic elastomer, the patterning of poly(D-gluconamidoethyl methacrylate) (PGAMA, glycopolymer) micro-domains on the PAMPS layer with photomask-assisted photolithography, followed by the generation of a phytohemagglutinin (PHA) array on the patterned surface through lectin–carbohydrate recognition. We demonstrate that the bi-component polymer-patterned surface with high lateral resolution is successfully fabricated; the PAMPS layer with patterned glycopolymer domains remains hydrophilic to resist non-specific plasma protein adsorption and cell adhesion; the PHA array on the patterned PGAMA domains induces nearly no platelet adhesion on the patterned surface, but shows high capability for capturing RBCs in the blood, and in addition, the captured RBCs maintain cellular integrity and function. Our work presented herein not only paves a new way for capturing RBCs from the blood, but also establishes a basic principle to capture non-adherent cells in the blood or biological fluid without damage.
研究领域:
材料科学
·
生物医学工程
·
细胞生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Liangting Shao,Jingwen Wang,Haijing Hu,Xinglian Xu,Huhu Wang
Microbial spoilage of meat products is a significant problem in the food industry. Aeromonas salmonicida is a significant microorganism responsible for spoilage in chilled meat. Its effector protein, hemagglutinin protease (Hap), has been identified as an effective substance for degrading meat proteins. The ability of Hap to hydrolyze myofibrillar proteins (MPs) in vitro demonstrated that Hap has obvious proteolytic activity, which could alter MPs’ tertiary structure, secondary structure, and sulfhydryl groups. Moreover, Hap could significantly degrade MPs, focusing primarily on myosin heavy chain (MHC) and actin. Active site analysis and molecular docking revealed that the active center of Hap was bound to MPs via hydrophobic interaction and hydrogen bonding. It may preferentially cleave peptide bonds between Gly44-Val45 in actin, and Ala825-Phe826 in MHC. These findings suggest that Hap may be involved in the spoilage mechanism of microorganisms and provide crucial insights into the mechanisms of meat spoilage induced by bacteria.
研究领域:
食品科学
·
微生物学
·
蛋白质化学
·
结构生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Bin Wang,Baojie Li,Huachuan Huang,Shuwei Yang,Dan Jian,Jing Liu,Keding Yan,Yanke Shan,Shouyu Wang,Fei Liu
Avian influenza virus (AIV) is a serious zoonotic disease causing severe damages to both poultry industry and human health. To rapidly detect AIV on-site with high sensitivity and accuracy, we design sensitive antibody fluorescence immunosorbent assay (SAFIA) on AIV H9N2 antibody. In SAFIA, hemagglutinin (HA1) protein coated sample chamber specifically binds targets but remarkably reduces non-specific absorption; Protein L coated polystyrene microsphere captures target through secondary antibody to significantly amplify the fluorescence signal; and a portable fluorescence counter automatically measures the fluorescence spot density for AIV H9N2 antibody detection. Proved by practical applications, SAFIA could probe AIV H9N2 antibody in high sensitivity and selectivity , and distinguish positive and negative serum samples in high accuracy. Additionally, SAFIA can rapidly detect AIV H9N2 antibody at room temperature only requiring simple operations as well as cost-effective and compact devices. Therefore, SAFIA is a potential new-generation tool in rapid on-site testing for agricultures.
研究领域:
兽医学
·
分子生物学
·
诊断技术
·
动物健康
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 23
·9145-9151
·
IF 3.069
Chanjuan Liu,Chao Li,Qingfeng Niu,Chao Cai,Guoyun Li,Guangli Yu
Carbohydrate microarrays provide a powerful tool for high-throughput analysis of carbohydrate-mediated interactions. In this work, they were fabricated on poly(2-hydroxyethyl methacrylate) (pHEMA)-cyanuric chloride (CC)-modified substrates for the analysis of carbohydrate–lectin interactions. The pHEMA polymer provides an anti-fouling surface and the CC linker allows the covalent immobilization of intact carbohydrates. Carbohydrates of different sizes (mannose, glucose, galactose, maltose, and dextran) were successfully immobilized on the proposed substrates and their recognition specificity with concanavalin A (ConA) was highly conserved. Compared with a 4-hydroxyphenylacetic acid (HPA)-CC-based microarray, the proposed pHEMA-CC-based microarray exhibits better anti-nonspecific adsorption ability, higher carbohydrate loading capability, stronger multivalent recognition and a lower limit of detection for carbohydrate and lectin without any further signal amplification. The application of the proposed microarray was validated by studying carbohydrate-influenza A virus H1N1 hemagglutinin interactions and fabricating microarrays containing carbohydrates with different properties. It is possible to extend it to study interactions of more carbohydrates and different lectins, and it will meet the wider needs in bioanalytical and biomedical application.
研究领域:
生物化学
·
分析化学
·
生物技术
·
分子生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）