Acta Biomaterialia
·
2019
·
IF 6.638
Xiao Yang,Wen Liu,Yifen Shi,Guanghui Xi,Mingshan Wang,Bin Liang,Yakai Feng,Xiangkui Ren,Changcan Shi
It is challenging for traditional hemostatic sponges to meet the clinic demand for both uncontrolled and noncompressible hemorrhage. With the aim to develop a rapid shape recovery material with both active and passive hemostatic performance, a dual-functional hemostatic sponge (TRAP-Sp) with a macroporous structure and good mechanical properties for controlling massive and noncompressible hemorrhage was prepared by chemically immobilizing thrombin-receptor-agonist-peptide (TRAP) onto a starch/polyethylene glycol (PEG) sponge. The TRAP2-Sp1 showed the best hemostatic performance among all samples in both rat artery uncontrollable hemorrhage and liver defect noncompressible hemorrhage models. When analyzing the hemostatic mechanism of TRAP-Sp, the high water absorption capacity of the sponge contributed to absorbing plasma, concentrating blood cells, and enhancing blood coagulation . After absorbing water, the shape-fixed TRAP-Sp with sufficient mechanical strength and high resilience can rapidly expand and apply pressure to the wound. TRAP immobilized on the sponge could activate the adhered platelets in an active pathway. Additionally, evaluation of cytotoxicity, hemolysis, and histology further highlighted the adequate biocompatibility of TRAP-Sp. With excellent hemostatic performance and biosafety, this sponge could be a potential candidate as a topical hemostatic agent for uncontrolled and noncompressible hemorrhage. Statement of Significance There is a need for innovative hemostatic materials for both uncontrolled and noncompressible hemorrhage. This manuscript describes a rapid shape recovery hemostatic sponge with both active and passive hemostatic performances synthesized by foaming technique, cross-linking reaction, and chemical immobilization of thrombin-receptor-agonist-peptide (TRAP). On contact with blood, the shape-fixed sponge can not only rapidly recover its original shape and concentrate platelets and RBCs but also activate the adhered platelets efficiently. The dual-functional sponge has excellent hemostatic efficacy in rat femoral artery hemorrhage and can control noncompressible hemorrhage in penetrating liver wound. Thus, we believe that this sponge could be a potential candidate as a topical hemostatic agent for uncontrolled and noncompressible hemorrhage.
研究领域:
生物材料学
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医学工程
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创伤修复
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
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2019
·
IF 1.398
Yufeng Sun,Jiameng Liu,Yatao Huang,Minmin Li,Jia Lu,Nuo Jin,Yan He,Bei Fan
Fresh kidney beans (Phaseolus vulgaris L.) are common green vegetable that are widely cultured and consumed worldwide. The benefits of fresh kidney beans are highly substantial, but at the same time, toxicity caused by phytohemagglutinin (PHA) has raised concerns. PHA from fresh kidney beans accounted for 40% of poisoning events induced by poisonous plants in China from 2004 to 2013. However, an understanding of PHA levels in fresh kidney beans remains elusive. An analysis method for PHA based on agglutination of PHA to erythrocyte cell was established. We investigated the effect of cultivar, bean part, maturity, and cooking method on PHA content in fresh kidney beans. We demonstrated significant differences in the distribution of PHA among various predominant, fresh Chinese kidney bean cultivars and parts. One major Chinese strain, Zihuayoudou, was found to contain the highest amount of PHA, which was abundant in fresh kidney bean seeds. Moreover, PHA concentrations were found to decrease with the maturity of the fresh product. Further, cooking methods had a significant effect on PHA levels in fresh kidney beans. PHA could be effectively removed when stir-frying for more than 18 min, or braising for more than 10 min. These findings provide insight into the PHA content of fresh kidney beans in China and will be helpful to guide food safety measures.
研究领域:
食品科学
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毒理学
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植物生物学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 7
·1361-1367
·
IF 4.872
Juanjuan Li,Xiaochun Hu,Shuo Shi,Yiwei Zhang,Tianming Yao
Early disease diagnosis is very important for the prevention or mitigation of metastasis. Effective and efficient methods are needed to improve the diagnosis and assessment of diseases. Thrombin is a biomarker for diagnosis of some diseases, such as pulmonary metastases and diseases associated with coagulation abnormalities. Lots of methods for detecting thrombin have been reported, however, most of them were based on a single aptamer and needed modification of aptamers, and label free sensors for thrombin detection based on an aptamer pair are challenging. In this work, we present three label free sensing platforms based on [Ru(bpy)2(o-mopip)]2+ (bpy = 2,2-bipyridine; o-mopip = 2-(2-methoxylphenyl)imidazo[4,5-f][1,10]phenanthroline) (OMO) and graphene oxide (GO) for thrombin detection. Thrombin detection using our three label free sensors is accomplished by specific target recognition based on aptamer pair TBA1/TBA2–thrombin, single aptamer TBA1–thrombin or TBA2–thrombin, respectively. Direct readouts of the target recognition is achieved by restoration of the fluorescence of OMO prequenched by GO. All of the three sensing platforms exhibited high sensitivity and selectivity. Furthermore, all sensing platforms were successfully applied to thrombin analysis in diluted bovine serum. According to the thrombin dependent response of the three platforms, the sensing platform based on aptamer pair TBA1 and TBA2 showed the highest sensitivity, widest linearity, best selectivity and recovery in diluted serum to thrombin. These results show that the sensing platform based on an aptamer pair have great potential for clinical diagnosis of disease-related biomarkers.
研究领域:
生物化学
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医学诊断
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分析化学
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
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2025
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IF 4.8
Sha Li,Si-Ming Yan,Li-Wei Zhang,Xiao-Yan Yang,Zhong Guo
Introduction: Polydopamine nanoparticles (PDA NPs) exhibit numerous outstanding characteristics, including simple preparation, broad light absorption, drug binding ability, excellent biocompatibility and adhesive properties, making them suitable for biomedical application. However, the limited information on their hemocompatibility may hinder their progression from laboratory research to clinical application.Methods: In this study, we investigated comprehensively the hemocompatibility of PDA NPs, assessed the effects of PDA NPs on red blood cells (RBCs) morphology and lysis, fibrinogen structure and conformation, blood coagulation, platelet activation, complement system activation, and organ toxicity.Results: The results indicated that PDA NPs can induce morphological changes and hemolysis in RBCs in a concentration-dependent manner. Interactions with fibrinogen suggested a disturbance in the protein’s microenvironment without significantly altering its secondary structure. This study also revealed that PDA NPs have a concentration-dependent effect on blood coagulation, platelet activation, and complement system activation. Additionally, PDA NPs showed no significant acute toxicity after intravenous injection.Conclusion: The findings offer important insights into the hemocompatibility of PDA NPs, which is essential for their safe and effective clinical use. Understanding their interactions with blood components is key to ensuring their compatibility in biomedical applications. These results are vital for guiding the development of PDA NPs for medical use, particularly in blood-contacting applications.
研究领域:
生物医学工程
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材料科学
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血液学
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL PHARMACOLOGY
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2017
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IF 4.581
Dongguo Lin,Yinzhu Luo,Guang Yang,Fangfang Li,Xiangkun Xie,Daiwei Chen,Lifang He,Jingyu Wang,Chunfeng Ye,Shengsheng Lu,Lin Lv,Shuwen Liu,Jian He
Influenza A viruses (IAVs) induce acute respiratory disease and cause significant morbidity and mortality throughout the world. With the emergence of drug-resistant viral strains, new and effective anti-IAV drugs with different modes of action are urgently needed. In this study, by conjugating cholesterol to the N-terminus of the short peptide KKWK, a lipopeptide named S-KKWK was created. The anti-IAV test indicated that S-KKWK and its derivatives displayed potent antiviral activities against a broad variety of influenza A viral strains including oseltamivir-resistant strains and clinically relevant isolates with IC 50 values ranging from 0.7 to 3.0 µM. An extensive mechanistic study showed that these peptides functioned as viral “entry blockers” by inhibiting the conformational rearrangements of HA2 subunit, thereby interrupting the fusion of virus-host cell membranes. Significantly, a computer-aided docking simulation and protein sequence alignment identified conserved residues in the stem region of HA2 as the possible binding site of S-KKWK, which may be employed as a potential drug target for designing anti-IAVs with a broad-spectrum of activity. By targeting this region, a potent anti-IAV agent was subsequently created. In addition, the anti-IAV activity of S-KKWK was assessed by experiments with influenza A virus-infected mice, in which S-KKWK reduced the mortality of infected animals and extended survival time significantly. Overall, in addition to providing a strategy for designing broad-spectrum anti-IAV agents, these results indicate that S-KKWK and its derivatives are prospective candidates for potent antivirals.
研究领域:
病毒学
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药物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Menghui Liu,Yang Sun,Yitong Zhou,Yanlv Chen,Chuandong He,Qing Zhao,Leye Yan,Jianan He,Jingpei Guo,Kecheng Liu,Yongchao Li,JiaYuan Lin,Weihua Liao,Hong Shan,Xin Peng
Hydrogels exhibit potential as embolic agents; however, external stimuli for gelation in vessels are difficult to operate or have poor biocompatibility, and shear-thinning hydrogels are under the risk of being washed away. Herein, a blood-triggering phase transformational hydrogel for endovascular embolization is reported. First, quaternized chitosan (QCS), tannic acid (TA) and iohexol (I) solutions are mixed to prepare a radiopaque and injectable QCS/TA/I (QTI) hydrogel. Upon contacting blood, Na + in blood can form coordination bonds with gel network to increase cross-linking densities, resulting in phase transformation from a soft and injectable hydrogel to a casted and stiffened QTI-blood hydrogel without any other operation. Moreover, the injection force and complete transformation time of the QTI hydrogels as well as the mechanical properties of their derived hydrogels are adjustable, which is helpful for customizing embolic agents according to different lesions. In animal experiments, QTI hydrogel has demonstrated its efficacy and safety in embolizing renal artery and ruptured femoral artery in rabbits, as well as the renal artery, profunda femoral artery (≈4 mm in diameter), and retia mirabilia with plexiform structure in pigs. This work not only develops a novel liquid embolic agent but also reveals a new strategy for blood-triggering phase transformation.
研究领域:
生物材料学
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介入医学
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产品关联
DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Yini Huangfu,Zhezhe Zhao,Xiang Liu,Jingrong Wang,Yufeng Zhang,Tingting Lan,Yonglan Wang,Chenxuan Wu,Ju Zhang,Pingsheng Huang,Chuangnian Zhang,Anjie Dong,Zujian Feng,Deling Kong,Weiwei Wang
Periodontitis is a destructive and chronic inflammatory disease initiated and sustained by multiple proinflammatory mediators. Current therapies mainly deal with bacteria elimination, but directly addressing the over-accumulated multiple inflammatory mediators in the periodontal microenvironment still remains a substantial challenge for regenerative periodontitis treatment. Herein, inspired by blood coagulation, an off-the-shelf artificial blood clot hydrogel encapsulated with platelet-rich plasma (PRP) is reported to mitigate the deteriorative inflammatory environment in periodontitis. The hydrogel (CCS-RSF@PRP) with a hierarchical fibers-interwoven network structure, in which the activated platelets are enriched, is structurally similar to the native blood clot. Functionally, in addition to the function of enriching and releasing growth factors, CCS-RSF@PRP can remarkably scavenge reactive oxygen species (ROS), neutralize endotoxin lipopolysaccharide (LPS), and proinflammatory cytokines (TNF-α, IFN-γ and IL-1β), inhibit M1 macrophage polarization and induce M2 macrophage polarization, thus blocking the chronic inflammatory feedback loop in the periodontitis. In rat periodontitis model, CCS-RSF@PRP hydrogel significantly expedits the repair of periodontium by normalizing the periodontal immune-environment. The work highlights the importance of local immunomodulation in the treatment of periodontitis, and the engineered PRP-derived hydrogel can mimic and expand the structure and function of native blood clot, holding great promise in treating chronic inflammatory diseases.
研究领域:
口腔医学
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生物材料学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2014
·
IF 4.517
Ting Bao,Wei Wen,Xiuhua Zhang,Shengfu Wang
In this work, a dual-signaling electrochemical aptasensor based on exonuclease-catalyzed target recycling was developed for thrombin detection. The proposed aptasensor coupled “signal-on” and “signal-off” strategies. As to the construction of the aptasensor, ferrocene (Fc) labeled thrombin binding aptamer (TBA) could perfectly hybridize with the methylene blue (MB) modified thiolated capture DNA to form double-stranded structure, hence emerged two different electrochemical signals. In the presence of thrombin, TBA could form a G-quadruplex structure with thrombin, leading to the dissociation of TBA from the duplex DNA and capture DNA formed hairpin structure. Exonuclease could selectively digest single-stranded TBA in G-quadruplex structure and released thrombin to realize target recycling. As a consequence, the electrochemical signal of MB enhanced significantly, which realized “signal on” strategy, meanwhile, the deoxidization peak current of Fc decreased distinctly, which realized “signal off” strategy. The employment of exonuclease and superposition of two signals significantly improved the sensitivity of the aptasensor. In this way, an aptasensor with high sensitivity, good stability and selectivity for quantitative detection of thrombin was constructed, which exhibited a good linear range from 5 pM to 50 nM with a detection limit of 0.9 pM (defined as S/N = 3). In addition, this design strategy could be applied to the detection of other proteins and small molecules.
研究领域:
生物化学
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医学检测
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分析化学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Guo Liu,Mingjuan Liao,Baoyan Guo,Qixin Kan,Shuangde Zhou,Konglong Feng,Wanhuang Lin,Yahui Huang,Jianyin Miao,Yong Cao
Castor ( Ricinus communis L.) meal, a by-product of castor oil production, is a high-quality protein source. Unfortunately, the toxic substance limits its application. Herein, a novel continuous phase transition extraction (CPTE) technology at pilot scale was explored for castor meal detoxification. The detoxification conditions were optimized using response surface methodology (RSM) as: treatment time of 3 h, extraction pressure of 0.16 MPa and temperature of 84.8°C. And the removal rate of allergens, hemagglutinin, and ricin reached 79.2 %, 99.8 % and 99.0 %, respectively. The protein content of castor meal increased from 38.4% to 39.0%. The nontoxicity nature of the treated caster meal was verified via an acute toxicity tests where the mean lethal dose was >5000 mg/kg body weight. Overall, CPTE offers a novel and efficient tool for castor meal detoxification, with the superiority of non-nutrient loss, it improved the application value of castor meal and reduced the risk of environmental pollution.
研究领域:
食品科学
·
植物学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 29
·3641-3647
·
IF 2.378
Chanjuan Liu,Chao Li,Guoyun Li,Guangli Yu
The specific interaction of carbohydrates and hemagglutinin (HA) on influenza A virus (IAV) is of great interest for carbohydrate-based anti-IAV drug discovery. Herein, a carbohydrate microarray-based method was developed for the determination of the specific interaction between carbohydrates and HA. Four marine acidic saccharides from algin (L-guluronic acid, GulA; L-diguluronic acid, GulA2; L-triguluronic acid, GulA3; and L-tetraguluronic acid, GulA4) were immobilized on poly(2-hydroxyethyl methacrylate)-cyanuric chloride-modified substrates, recently reported by us, to form a microarray. GulA, GulA2, GulA3, and GulA4 all showed specific binding with HA from influenza A H1N1 subtype and exhibited increased binding affinity. Molecular docking was adopted to simulate the complexes formed by investigated saccharides and HA to gain better insight into their binding behaviors. The reliability of the carbohydrate microarray-based assay was also verified by cytopathic effect inhibition assay and surface plasmon resonance analysis. By adopting this microarray-based method, the consumption of saccharides could be reduced to the pmol level and the limit of detection of HA could be reduced to 8.4 nM without any further signal amplification. This carbohydrate microarray-based assay is thus an ideal tool for studying specific interactions of carbohydrates and HA or other proteins, and will provide a promising platform for carbohydrate-based drug discovery and development.
研究领域:
病毒学
·
生物化学
·
分子生物学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
Brazilian Journal of Pharmaceutical Sciences
·
2023
·
IF 1.3
Mei Jian feng,Cai Shao fen,Yi Yu,Wang Xu dong,Ying Guo qing
Serrapeptase, a proteolytic enzyme, has been used for the adjuvant treatment of many diseases. However, its fibrinolytic activity is still uncertain. Herein, the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects were investigated. The results showed that the fibrinolytic activity of serrapeptase was 1295 U/mg, and the specific activity was 3867 U/mg of protein when its proteolytic activity toward casein was 2800 U/mg. The optimum temperature and pH for serrapeptase activity were 37-40°C and 9.0, respectively. At 1 mmol/L, Zn2+, Mn2+ and Fe2+ could activate the fibrinolytic activity of serrapeptase, while K+, Cu2+, sodium dodecyl sulfate (SDS) and ethylene diamine tetraacetic acid (EDTA) inhibited it. In vitro tests showed that serrapeptase could completely prevent blood coagulation at 150 U/mL, and the percentage of blood clot lysis reached 96.6% at 37°C after 4 h at 300 U/mL. These results indicate that serrapeptase has excellent fibrinolytic activity, and can be used as a health food or candidate drug for the prevention or treatment of thrombotic diseases.
研究领域:
生物化学
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医学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·Vol. 13
·期 23
·2400993
·
IF 10
Qiao Yu,Wenjie Wang,Ningyue Deng,Baihai Su,Weifeng Zhao,Changsheng Zhao
Ideal hemostatic materials for the emergency rescue of war and traffic accident sufferers are essential to significantly control hemorrhage, reduce patient discomfort, and improve the survival ratio. However, most hemostats absorb blood quickly in contact with the wound; and then, adhere to blood clots, resulting in breaking scabs and tearing the wound when the materials are removed. Herein, an effective Janus amphipathic hemostatic dressing (Fiber@Gel/Ca 2+ /KL) with a fiber layer (polylactic acid/carboxymethyl chitosan) and a hydrogel layer (polyvinyl alcohol, carboxymethyl chitosan, Ca 2+ , and kaolin) is reported. Such a composite dressing unidirectionally drains the excessive serum from its hydrophobic side (fiber layer) to its hydrophilic side (hydrogel layer), so-called self-pumping, thereby further concentrating coagulated factors (including red blood cells and platelets). Further, Ca 2+ diffused from the hydrogel layer subsequently activates platelets and coagulation cascade. Besides, the Fiber@Gel/Ca 2+ /KL exhibits specific blood-clot anti-adhesion property on the fiber layer, making the dressing easily and safely peel off from the wound. It is believed that this novel hemostatic dressing with good hemostatic performance, easy clots removal, and excellent biocompatibility is expected to be used as a safe and efficient hemostatic dressing in clinical applications.
研究领域:
医学材料学
·
创伤急救
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）