PLoS One
·
2015
·Vol. 10
·期 10
·e0140560
·
IF 3.234
Paul Kyu Han,Jeffrey W. Barker,Ki Hwan Kim,Seung Hong Choi,Kyongtae Ty Bae,Sung-Hong Park
The recent blood flow and magnetization transfer (MT) technique termed alternate ascending/descending directional navigation (ALADDIN) achieves the contrast using interslice blood flow and MT effects with no separate preparation RF pulse, thereby potentially overcoming limitations of conventional methods. In this study, we examined the signal characteristics of ALADDIN as a simultaneous blood flow and MT imaging strategy, by comparing it with pseudo-continuous ASL (pCASL) and conventional MT asymmetry (MTA) methods, all of which had the same bSSFP readout. Bloch-equation simulations and experiments showed ALADDIN perfusion signals increased with flip angle, whereas MTA signals peaked at flip angle around 45°−60°. ALADDIN provided signals comparable to those of pCASL and conventional MTA methods emulating the first, second, and third prior slices of ALADDIN under the same scan conditions, suggesting ALADDIN signals to be superposition of signals from multiple labeling planes. The quantitative cerebral blood flow signals from a modified continuous ASL model overestimated the perfusion signals compared to those measured with a pulsed ASL method. Simultaneous mapping of blood flow, MTA, and MT ratio in the whole brain is feasible with ALADDIN within a clinically reasonable time, which can potentially help diagnosis of various diseases.
研究领域:
医学影像
·
神经影像
·
磁共振成像 (MRI)
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
Biology-Basel
·
2025
·Vol. 14
·期 1
·51
·
IF 3.5
Ferry Saputra,Tzu-Ming Tseng,Franelyne P. Casuga,Yu-Heng Lai,Chih-Hsin Hung,Chung-Der Hsiao
Simple SummaryMany studies have been developed to calculate the blood flow velocity in adult zebrafish. However, the current method requires high-cost equipment a lot of training before it can be replicated. In this study, we provide a simple, cheap, and non-invasive method to enable the measurement of zebrafish blood flow velocity. By applying the same method as the one previously developed for larvae zebrafish, blood flow velocity can be measured and its sensitivity validated by performing an experiment under several ambient temperature conditions, as well as the presence of a toxicant. The applicability of this method was also checked by applying the same method to other fish. Overall, this method provides an easy, cheap, and robust method that can be used by low- to medium-scale laboratories to contribute to knowledge in the relevant fields.Blood flow is an important physiological endpoint to measure cardiovascular performance in animals. Because of their innate transparent bodies, zebrafish is an excellent animal model for assessing in vivo cardiovascular performance. Previously, various helpful methods for measuring blood flow in zebrafish larvae were discovered and developed. However, an optimized method to measure blood flow in adult zebrafish has not been reported. In this paper, the tail fin region was selected as target for blood flow measurements using the Trackmate method, provided by ImageJ platform. Based on power statistic calculations, the aortic vessel at the tail base was selected, and other parameters, such as ambient temperature, were investigated for method standardization, in order to minimize experimental variation. The method was also validated using fenpropathrin and ponatinib, which showed some cardiac alterations in a previous zebrafish study. We also checked the versatility of this method by following the same setup in black tetra and medaka and found that this method performed well. However, our results show that heavy pigmentation, like that found in tiger barb, and overlapping vessels, like those in parrot fish, make it hard for this method to perform well. Overall, an optimized protocol was used for the first time to measure blood flow velocity in adult wild-type zebrafish without the aid of transgenic lines or fluorescent dye.
研究领域:
生物学
·
医学
·
毒理学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inventions
·
2019
·Vol. 4
·期 4
·65
·
IF 0
Fiorency Santoso,Bonifasius Putera Sampurna,Yu-Heng Lai,Sung-Tzu Liang,Erwei Hao,Jung-Ren Chen,Chung-Der Hsiao
This study aimed to develop a simple and cost-effective method to measure blood flow in zebrafish by using an image-based approach. Three days post fertilization (dpf) zebrafish embryos were mounted with methylcellulose and subjected to video recording for tracking blood flow under an inverted microscope equipped with a high-speed CCD camera. In addition, Hoffman lens was used to enhance the blood cell contrast. The red blood cell movement was tracked by using the TrackMate plug-in in the ImageJ image processing program. Moreover, Stack Difference and Time Series Analyzer plug-in were used to detect dynamic pixel changes over time to calculate the blood flow rate. In addition to blood flow velocity and heart rate, the effect of drug treatments on other cardiovascular function parameters, such as stroke volume and cardiac output remains to be explored. Therefore, by using this method, the potential side effects on the cardiovascular performance of ethyl 3-aminobenzoate methanesulfonate (MS222) and 3-isobutyl-1-methylxanthine (IBMX) were evaluated. MS222 is a common anesthetic, while IBMX is a naturally occurring methylxanthine. Compared to normal embryos, MS222- and IBMX-treated embryos had a reduced blood flow velocity by approximately 72% and 58%, respectively. This study showed that MS222 significantly decreased the heart rate, whereas IBMX increased the heart rate. Moreover, it also demonstrated that MS222 treatment reduced 50% of the stroke volume and cardiac output. While IBMX decreased the stroke volume only. The results are in line with previous studies that used expensive instruments and complicated software analysis to assess cardiovascular function. In conclusion, a simple and low-cost method can be used to study blood flow in zebrafish embryos for compound screening. Furthermore, it could provide a precise measurement of clinically relevant cardiac functions, specifically heart rate, stroke volume, and cardiac output.Keywords:blood flow tracking;ImageJ;zebrafish;IBMX;MS222;cardiovascular function assessment
研究领域:
发育生物学
·
毒理学
✓ 已验证
产品关联
DOI
Progress in Natural Science-Materials International
·
2024
·
IF 4.8
Qinli Ye,Jinhui Lin,Guoxiang Wang,Shuwen Zeng,Tao Jiang,Xiang Gao,Muhammad Danang Birowosuto,Wei Xiong,Chenjie Gu
Infrared thermography technology is a noninvasiveness and real-time imaging method, it has attracted enormous attentions for blood flow imaging. However, the implementation of infrared thermography technology is still limited by the poor imaging quality caused by the low temperature discrimination between the target blood vessel and background. Herein, we reported a flexible and low-cost MXene based photothermal microheater for enhancing the infrared imaging of blood flow. In the experiment, MXene films with specific thickness was prepared by vacuum filtering different concentrations of MXene colloidal solutions. Thereafter, the microheaters were fabricated by assembling the MXene films on the PU tape/PDMS substrate, and then sealed with another layer of pure PDMS film on the top of MXene film. The photothermal performance of the microheaters were evaluated by using an 808-nm laser as the exciting source. Experimental results show that the optimal microheater (MXene content: 17.5 mg) can reach a safe temperature of 41.5 °C with 43.9 s under the light irradiation of 100 mW·cm −2 . Moreover, it can be stabilized at the equilibrium temperate for over 25 min. Finally, the above optimal microheater was used to accurately heat the simulated blood vessel. It shows that temperature difference of 3.3 °C can be induced between the heated blood vessel and environment. As a result, it significantly enhances the quality of the infrared imaging of the blood flow in the simulated vascular network.
研究领域:
生物医学工程
·
材料科学
·
医学影像学
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2018
·Vol. 32
·期 6
·e4210
·
IF 1.688
Weiguo Liao,Xiaojie Huang,Yongxin Yin,Bin Liu,Runzhi Zhu
Tetramethylpyrazine (TMP) has been widely used in the treatment of ischemic cerebrovascular disease. However, the mechanism of TMP and how to increase its bioavailability need to be further explored. In our study, an in vivo microdialysis sampling technique coupled with ultra-performance liquid chromatography–mass spectrometry method was developed to investigate the pharmacokinetic properties of TMP and its interaction with different doses of borneol (BO) in rats. Linearity of TMP in brain and blood dialysates exhibited good linear relationships over the concentration range of 0.991–555.14 ng/mL. The specificity, linearity, accuracy, precision, matrix effect and stability were within acceptable ranges. The results demonstrated that BO had a marked impact on the pharmacokinetic properties of TMP. After co-administration, the areas under the concentration–time curve (AUC) of TMP in brain and blood were significantly increased. Meanwhile, the peak concentration of TMP in brain was also enhanced. The AUC Brain /AUC Blood of TMP, increased from 44% to 56 and 60.8% after co-administration with BO (15 and 30 mg/kg). The pharmacodynamic results showed that TMP co-administration with BO enhanced the cerebral blood flow during the period of ischemia and reduced the infarct volume. Overall, it might be an effective way to treat stroke to use TMP co-administered with BO.
研究领域:
药理学
·
神经科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Communications
·
2025
·
IF 4.5
Yi'an Chen,Mengxue Zhou,Jingyue Wang,Yunji Fei,Xiaoling Yan,Xin Hao,Ansha Zhao
Atherosclerotic plaques can be eliminated using approaches such as endarterectomy. However, intimal damage resulting from surgery is frequently challenging to address. Traditional surgical techniques often incorporate foreign substances such as modified stents or drugs to counter the consequences of intimal defects. Such treatment makes it difficult to repair vascular defects in endothelial segments. It is prone to cause problems such as hyperplasia and abnormal blood supply due to vascular endothelial defects. In this study, hyaluronic acid (HA), a natural extracellular mechanism component that is not prone to actively induce coagulation, was employed as a biomimetic material for intimal repair. Through chemical grafting modification, the gel was endowed with rapid photocuring performance within 3 s, and the scientific attributes of the gel-series materials were enhanced. The additional incorporation of Cu 2+ into the gel-prepared HMN-Cu 2+ hydrogel augmented the anticoagulant effect of the material while enhancing blood cell compatibility. The results of material science experiments indicate that HMN gel can adhere stably to various biological interfaces in a fluid environment mimicking blood flow, and its swelling and degradation properties are appropriate. Cell experiments and blood evaluation demonstrated that the gel exhibited favorable erythrocyte compatibility and anticoagulant and endothelial spreading capabilities during intimal repair. The gel has the potential to substitute the intima barrier, improve the intima repair environment, and facilitate subsequent intima repair. It can effectively resolve the problem of intima layer irreparability during novel low-damage vascular surgeries such as intima stripping and plaque rotation.
研究领域:
生物材料学
·
医学工程
·
血管外科
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Science
·
2019
·Vol. 7
·期 4
·1507-1515
·
IF 5.251
Yongjing Li,Jiaxun Wan,Fang Wang,Jia Guo,Changchun Wang
The clinical applications of particulate drug delivery systems have demonstrated limited treatment outcomes, which is largely attributable to the elimination of such systems by the immune system, especially in the liver. Inspired by the mechanism of nanomaterial clearance by the liver, we designed a new anticancer auxiliary delivery system by introducing norepinephrine loaded poly(acrylic acid) nanogels as angiotonics. The auxilliary system effectively decreased the liver uptake of nanodrugs by increasing the liver blood flow rate. With administration of the as-prepared norepinephrine-loaded poly(acrylic acid) nanogels, the blood perfusion amount increased significantly by 177.0% (i.e. 2.77 times) as observed directly by ultrasonic imaging, indicating an increased blood flow rate in the liver. Since the blood flow rate plays a key role in nanomaterial clearance in the liver, nanodrug clearance should be changed by modulation of the blood flow. Our in vivo experimental results clearly showed the enhancement of nanodrug efficiency with this two-step treatment, with a 52% improvement in plasma drug concentration, obvious drug accumulation in the tumor, and significant antitumor effects. These results indicate that a pre-conditioning strategy involving norepinephrine-loaded poly(acrylic acid) nanogels can serve as an ideal route for reducing nanodrug clearance by the liver.
研究领域:
药学
·
肿瘤学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Dental Sciences
·
2024
·
IF 3.4
Xin-Yi Cheng,Pei-Hui Zou,Yi-Ming Ma,Yu Cai,Qiao Shi,Jia Liu,Qing-Xian Luan
Background/purpose Periodontitis is associated with systemic health. One of the underlying mechanisms is the translocation of periodontal pathogens, among which Porphyromonas gingivalis ( Pg ) is the most common. Here, we aimed to illustrate the biodistribution and dynamics of Pg from gingiva to multiple organs through blood circulation. Materials and methods Pg tagged by Cyanine 7 (Cy7- Pg ) was injected into the gingiva of healthy and periodontitis mice. In vivo imaging system (IVIS) was applied to monitor the distribution of Cy7- Pg in multiple organs which were isolated at serial timepoints. Polymerase chain reaction (PCR) was conducted to determine the Pg DNA copies in the gingiva, blood and organs. Cy7- Pg in the gingiva and organs was also confirmed by frozen section staining. Furthermore, to figure out whether the bacteremia derived from oral-gut axis, mice received gavage of Cy7- Pg . Then the blood and organ samples were detected in the similar way as above. Results Intra-gingival injection induced larger amounts of Cy7- Pg accumulating in the gingiva of periodontitis mice ( P < 0.05) as confirmed by above three methods. Twenty minutes after injection, Pg DNA copies in the blood of periodontitis group were 36.3-fold higher than healthy group ( P < 0.05). IVIS results, combined with PCR and frozen sections, demonstrated periodontitis induced longer retention with higher amounts of Cy7- Pg in the periodontitis group. Pg was enriched more significantly in the liver for the longer duration than the kidney and pancreas. Conclusion Our study showed Pg , which accumulated in the gingiva, could translocate through blood circulation to multiple organs with varied duration and amounts.
研究领域:
口腔医学
·
感染性疾病
·
分子影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Tissue Engineering and Regenerative Medicine
·
2018
·Vol. 12
·期 12
·2266-2276
·
IF 4.089
Jing Liu,Chengpan Li,Shaohui Cheng,Shengnan Ya,Dayong Gao,Weiping Ding
In vitro engineering of liver tissue is a rapidly developing field for various biomedical applications. However, liver tissue culture is currently performed on only a small scale with a low density of hepatocytes. In this study, a simple design was introduced in a liver microsystem to enhance the transport of nutrients (e.g., oxygen and glucose) for the three-dimensional large-scale, high-density culture of hepatocytes. In this design, convection across the cell culture zone was generated to mimic sinusoid blood flow (SBF) based on the pressure difference between two fluids flowing in a countercurrent manner on either side of the cell culture zone. First, the distributions of living and dead cells in different culture subzones under various perfusion flow rates were observed, analysed, and compared. Then, the enhanced transport of nutrients was experimentally validated in relation to the viability of cells and theoretically explained by comparing the fluid velocity and oxygen concentration distribution in the cell culture zone in counterflow and coflow modes. Finally, the functions of the SBF-mimicked liver microsystem were assessed on the basis of specific metabolites, synthesized proteins, and bilirubin detoxification of hepatocytes, with collagen and alginate as extracellular matrices. Under this design, the density of hepatocytes cultured at the 3-mm-thickness scale reached ~7 × 10 7 cells/ml on Day 7, and the metabolism and detoxification functions of the cells worked well. In addition, a liver rope-like structure and sphere-like clusters of cells were observed. This work provides insight for the design of a bionic liver microsystem.
研究领域:
组织工程
·
生物医学工程
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Chenchen Liu,Xiaoming Liu,Zhihao Wei,Zong Chang,Yaowei Bai,Pei Zeng,Qi Cao,Changjun Tie,Ziqiao Lei,Peng Sun,Huageng Liang,Qinchao Sun,Xiaoping Zhang
Magnetic resonance angiography (MRA) contrast agents are extensively utilized in clinical practice due to their capability of improving the image resolution and sensitivity. However, the clinically approved MRA contrast agents have the disadvantages of a limited acquisition time window and high dose administration for effective imaging. Herein, albumin-coated gadolinium-based nanoparticles (BSA-Gd) were meticulously developed for in vivo ultrahigh-resolution MRA. Compared to Gd-DTPA, BSA-Gd exhibits a significantly higher longitudinal relaxivity (r1 = 76.7 mM–1 s–1), nearly 16-fold greater than that of Gd-DTPA, and an extended blood circulation time (t1/2 = 40 min), enabling a dramatically enhanced high-resolution imaging of microvessels (sub-200 μm) and low dose imaging (about 1/16 that of Gd-DTPA). Furthermore, the clinically significant fine vessels were successfully mapped in large mammals, including a circle of Willis, kidney and liver vascular branches, tumor vessels, and differentiated arteries from veins using dynamic contrast-enhanced MRA BSA-Gd, and have superior imaging capability and biocompatibility, and their clinical applications hold substantial promise.
研究领域:
医学影像学
·
纳米医学
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
npj Flexible Electronics
·
2024
·Vol. 8
·期 1
·1-16
·
IF 12.3
Deng Mengkang,Du Chentao,Fang Jiayuan,Xu Changshun,Guo Chenhui,Huang Jiantao,Li Kang,Chen Lei,Zhang Yuan-Ting,Chang Yu,Pan Tingrui
Continuous hemodynamic monitoring in a wearable means can play a crucial role in managing hypertension and preventing catastrophic cardiovascular events. In this study, we have described the fully wearable tonometric device, referred to as flexible adaptive sensing tonometry (FAST), which is capable of continuous and accurate monitoring of hemodynamic parameters within the medical-grade precision. In particular, the FAST system integrates a 1 × 8 unit array of highly sensitive and highly flexible iontronic sensing (FITS) with 1 mm spatial resolution and a closed-loop motion system. The flexible tonometric architecture has been used to determine the radial arterial position with high sensitivity and high conformability, which simplifies the biaxial searching process of the traditional applanation tonometry into a highly efficient uniaxial applanation while keeping the medical-precision assessments. Importantly, a self-calibration algorithm can be automatically implemented during the applanation process, from which the intra-arterial blood pressure wave can be continuously predicted within the medical-grade precision, and subsequently, multi-parametric hemodynamic analysis can be performed in real-time. Experimental validations on health volunteers have demonstrated that the FAST measurements are all within the required accuracy of the clinical standards for continuous pulse wave assessments, blood pressure monitoring as well as other key hemodynamic parameter evaluations. Therefore, the FAST system, by integrating the flexible iontronic sensing array, provides a real-time, medical-grade hemodynamic monitoring solution in a continuously wearable manner, from which remote patient-centered monitoring can be delivered with both medical precision and convenience.
研究领域:
生物医学工程
·
医疗器械
·
健康监测
✓ 已验证
产品关联
DOI
高影响因子
Bioengineered
·
2021
·
IF 3.269
Yingnan Dai,Yeping Chen,Guoqian Wei,Li Zha,Xueqi Li
Ivabradine (Iva), a heart rate reducing agent that specifically inhibits the pacemaker I(f) ionic current, has been demonstrated to be cardioprotective in many cardiovascular diseases. Autophagy is an evolutionarily conserved metabolic process that regulates cardiac homeostasis. This study is aimed to explore whether autophagy is functionally involved in the cardioprotective effect of Iva in a rat model of myocardial infarction (MI). We observed that Iva treatment (po, 10 mg/kg/day) showed significant recovery on the hemodynamics parameters in MI rats, including left ventricular systolic pressure, left ventricular end diastolic pressure, and maximal ascending/descending rate of left ventricular pressure. Also, Iva treatment dramatically decreased infarct size, inhibited myocardial apoptosis, and reduced the levels of pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in MI rats. Moreover, Iva treatment enhanced autophagy and inhibited PI3K/AKT/mTOR/p70S6K pathway in MI rats. Simultaneously, we observed that autophagy enhancer rapamycin (ip, 10 mg/kg/day) showed similar cardioprotective effects with Iva. Furthermore, we observed that addition of autophagy inhibitor 3-methyladenine (ip, 10 mg/kg/day) counteracted the therapeutic effect of Iva, addressing that Iva attenuated post-MI cardiac injury by enhancing autophagy. In summary, these findings demonstrated that Iva attenuated MI in rats by enhancing autophagy, and PI3K/AKT/mTOR/p70S6K pathway might be involved in the process. Autophagy activation by Iva may be a potential therapeutic strategy for the treatment of MI.
研究领域:
心脏病学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）