POULTRY SCIENCE
·
2020
·
IF 2.659
Jingle Jiang,Siyi Liu,Tuniyaz Jamal,Tengxin Ding,Lina Qi,Zengpeng Lv,Debing Yu,Fangxiong Shi
The objective of this study was to investigate the effects of dietary 3 kinds of sweeteners supplementation on growth performance, serum biochemicals, and jejunal physiological functions of broiler chickens for 21 D. A total of one hundred ninety-two 1-day-old male Ross 308 broiler chicks were randomly divided into 4 treatments with 6 replicates for each treatment. The treatments were basal diet ( CON ), a basal diet supplemented with 250 mg/kg stevioside ( STE ), a basal diet supplemented with 100 mg/kg sucralose ( SUC ), and a basal diet supplemented with 600 mg/kg saccharin sodium ( SAC ). All birds were housed in 3-level battery cages. The results showed that dietary STE supplementation increased ( P < 0.05) growth performance, serum total protein, serum albumin, and jejunal antioxidant capacity of broiler chickens. Both SUC and SAC supplementation decreased ( P < 0.05) serum total protein and albumin. Dietary SAC supplementation impaired the intestinal integrity, permeability, and mucus layer of the jejunum in broiler chickens. In addition, SAC supplementation elevated ( P < 0.05) the transcription expression level of jejunal bitter taste receptors and induced excessive jejunal apoptosis. Our data suggest that STE could be potentially applied as a growth-promoting and antioxidant feed additive in broiler chickens. Whereas, dietary supplementation with high level SAC has side-effects on the jejunal physiological functions of broiler chickens.
研究领域:
动物营养学
·
家禽科学
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DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2017
·Vol. 23
·期 5
·1076-1085
·
IF 1.665
Q. Zou,Y. Huang,J. Cao,H. Zhao,G. Wang,Y. Li,Q. Pan
The present research evaluated the effects of four feeding stimulants on feed intake (FI), growth performance, body composition, serum biochemical parameters, digestive enzyme activities and appetite-related gene expression for juvenile GIFT tilapia. Five experimental high plant-based diets (with 0, 0.4 g kg −1 dimethyl-β-propiothetin (DMPT), 0.6 g kg −1 dimethylthetin (DMT), 1.8 g kg −1 tryptophan (Trp) and 6 g kg −1 betaine (Bet), respectively supplemented) were fed to juvenile GIFT tilapia ( Oreochromis sp .) (3.34 ± 0.01 g) for 8 weeks. The results showed that FI was significantly increased by Trp and DMT. The highest specific growth rate (SGR) and protein efficiency ratio (PER) was observed for fish fed Bet diet, followed by DMT. Serum triglyceride was significantly increased by DMPT. Fish fed Bet diet showed significantly higher intestinal protease, liver protease and intestinal amylase activities. The neuropeptide Y (NPY) mRNA expression in brain was significantly improved by Trp, and ghrelin mRNA expression in stomach was significantly increased by DMT. It is concluded that supplementation of Trp or DMT could act as an effective feeding stimulants (FS) in high plant-based diets for juvenile GIFT tilapia, which might be associated with the relatively higher orexigenic (NPY or ghrelin) genes expression, and supplementation of Bet could improve SGR, PER and reduce feed conversion ratio (FCR) of juvenile GIFT tilapia.
研究领域:
水产养殖学
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营养学
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分子生物学
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DOI
高影响因子
EUROPEAN JOURNAL OF NUTRITION
·
2023
·Vol. 62
·期 6
·2687-2703
·
IF 5
Zhuang Kejin,Meng Weihong,Shu Xin,Liang Defu,Wang Lidong,Zhang Dongjie
Purpose When blended, animal and plant proteins can complement each other in terms of amino acid composition and release time. In this study, we investigated whether the blended protein diet has a better feeding effect than the single protein diet, and to reveal the differences in growth and intestinal microbiota composition caused by the blended protein diet. Methods Forty Sprague Dawley (SD) rats received diets with different protein sources, including casein (C), whey protein (WP), black soybean protein (BSP), and black soybean-whey blended protein (BS-WP), for eight weeks. To investigate the effects of blended protein supplement on gut microbiota and metabolites, we performed a high throughput 16S rDNA sequencing and fecal metabolomics profiling. In addition, we determined growth and serum biochemical indices, and conducted intestinal morphology analyses. Results Compared to those in the BSP and WP groups, the daily body weight gain and feed conversion efficiency increased in the BS-WP group. Serum biochemical indices indicated that the protein utilization efficiency of the WP and BS-WP groups was relatively high, and the BS-WP blended protein diet improved the protein adoption rate. The BS-WP blended protein diet also improved intestinal tissue morphology and promoted intestinal villi development compared to the single protein diets. Furthermore, dietary protein altered the composition of gut microbiota, the gut microbial diversity of rats fed with the BS-WP diet was significantly ( P < 0.05) higher than that of the other groups. The difference in dietary protein corresponded with an alteration of fecal amino acids and their metabolites, and tryptophan and tyrosine metabolism were the key mechanisms leading to the changes in fecal microbial composition. Conclusion Dietary protein sources played an important role in the growth and development of rats by influencing intestinal metabolism and microbial composition. The BS-WP blended protein diet was more conducive to nutrient absorption than the single protein diet. Furthermore, blended protein increased the diversity of intestinal microbes and aided the establishment of intestinal barrier function.
研究领域:
营养学
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微生物学
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代谢组学
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动物科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Ge Sun,Yubiao Huang,Xiaoyu Cheng,Lijun Fang,Chunling Wang,Yinlong Zhang
Anlotinib, a multitargeted tyrosine kinase inhibitor (TKI) with a dual effect of inhibiting both tumor angiogenesis and tumor cell proliferation, holds great promise in cancer therapy. However, its clinical application has been limited due to off-target side effects. With the rapid development of drug delivery systems, there is increasing potential to improve therapeutic efficacy while reducing the toxicity associated with free drugs. Among these, liposomes stand out due to their excellent biocompatibility, and several liposomal drug formulations have been approved by the U.S. Food and Drug Administration (FDA). In this study, we prepared anlotinib-loaded liposomes (designated as Lipo-Anlotinib) using the ammonium sulfate gradient method, aiming to enhance its clinical applicability through a more efficient delivery system. The antitumor activity of Lipo-Anlotinib was evaluated in vitro against three cell lines (A549, 4T1, and CT26). Additionally, Lipo-Anlotinib exhibited improved therapeutic efficacy over free anlotinib in a 4T1 subcutaneous tumor model. Serum biochemical analysis and histological examination revealed that Lipo-Anlotinib exhibited reduced toxicity in comparison to free anlotinib. This liposomal formulation offers a promising approach to enhancing the antitumor efficacy while mitigating the side effects of anlotinib.
研究领域:
药理学
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肿瘤学
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纳米技术
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生物医学工程
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癌症治疗
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产品关联
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2022
·
IF 5.062
Jiarong Hang,Yu Chen,Pengyao Tian,Ruonan Yu,Miao Wang,Min Zhao
Niclosamide (NIC) has been found to have outstanding antitumour activity in recent years, but its clinical application has been limited by its poor water solubility. In this study, nanomicelles (PEG 2K -Fmoc-Ibuprofen micelles , PEG 2K -FIbu) were prepared as carriers to improve the solubility and bioavailability of NIC. Nuclear magnetic resonance (NMR) confirmed its successful preparation. The drug-carrying micelles (PEG 2K -FIbu/NIC) were then prepared using a thin film dispersion method. PEG 2K -FIbu and PEG 2K -FIbu/NIC were analysed in detail in terms of particle size, polymer dispersion index, stability and encapsulation efficiency. Finally, the in vivo effects of PEG 2K -FIbu/NIC were tested in a mouse liver cancer model. The particle size of PEG 2K -FIbu was 11.11 nm. The encapsulation rate was 70.4% and the tumour inhibition rate was 55.98%. Tumours were significantly reduced and damage to the liver was greatly reduced. The histopathological sections and serum biochemical indices also showed that the symptoms were significantly improved after administration. In summary, PEG 2K -FIbu/NIC is a potential drug delivery system of NIC to effectively treat liver cancer, providing an effective dosage form for NIC.
研究领域:
药学
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癌症研究
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药物递送系统
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Water Biology and Security
·
2023
·
IF 0
Chaolin Jiang,Xingchen Huo,Lingjie Tang,Meidi Hu,Chunrong Yang,Daji Luo,Jianguo Su
Grass carp hemorrhagic disease caused by grass carp reovirus (GCRV) results in significant economic losses to the global grass carp aquaculture industry. Oral vaccination is an ideal choice for disease precaution in aquaculture. However, oral vaccine can be degraded in the gut. Therefore, the selection of loading materials is essential. In this study, the S6 and S7 fragments (encoding the outer capsid protein VP4 and fibronectin VP56 of GCRV) and grass carp interferons (IFNs), including IFN1, IFN3, and IFNγ2 were used to create DNA vaccines and adjuvants based on pcDNA3.1, respectively. The oral DNA vaccine was encapsulated in poly(lactic-co-glycolic acid) (PLGA) and polyvinyl alcohol (PVA) with IFNs. The PLGA-PVA (PP) nano-microspheres were prepared by double emulsion-solvent evaporation technique. Using transmission electron microscopy and dynamic light scattering assays, it was determined the vaccines had a spherical structure with uniform particle size (643.5 ± 35.3 nm). The nano-microspheres possessed excellent encapsulation efficiency (81.6 ± 2.6%) and loading rate (0.54 ± 0.02%), and simultaneously exhibited negligible hemolytic activity and cell toxicity. The protection rate and tissue viral loads post-GCRV challenge in grass carp were assessed. The oral PP nano-microsphere with pVP4 + pIFN1 (PP41) vaccine increased protection rate by 44% compared with the control group and was correlated with relatively low viral loads in the spleen, head kidney, and hindgut. Further, three crucial serum biochemical indexes, total superoxide dismutase (TSOD), complement C3 (C3), and lysozyme (LZM), were also dramatically increased. Furthermore, mRNA expressions of representative immune-related genes (IgM, IFN1, IFNγ2, MHC-Ⅰ, and CD8α) in the head kidney and spleen were significantly enhanced. In addition, mRNA expression of IgT was significantly boosted in the hindgut. The results indicate that DNA vaccine capsulated with PP is effective against GCRV infection. The present study provides insights into a prospective strategy for oral vaccine development in aquaculture.
研究领域:
水产养殖
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免疫学
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病毒学
·
兽医学
✓ 已验证
DOI
JOURNAL OF ETHNOPHARMACOLOGY
·
2022
·
IF 5.195
Zhen Xue,Lingxin Zhuo,Bowen Zhang,Lingmeng Zhu,Xinran Xiang,Chunxia Zhang,Wenyuan Liu,Guangguo Tan,Wenting Liao
Ethnopharmacological relevance Qifu decoction (QFD) is a famous traditional Chinese medicine (TCM) composed of Astragali Radix (HuangQi) and Aconiti Lateralis Radix Praeparaia (Fuzi), which can alleviate doxorubicin (DOX)-induced cardiotoxicity (DIC). However, its protective mechanism remains obscured. Aim of the study The present study aimed to uncover the cardioprotective mechanism and the synergistic effect of QFD against DIC in mice. Materials and methods The cardioprotective activity of QFD against DIC was assessed by electrocardiogram, serum biochemical assays and histopathology. Mass spectrometry-based metabolomic approach was conducted to elucidate the preventive mechanisms of QFD, HuangQi decoction (HQD), and Fuzi decoction (FZD) against DIC. QFD, HQD, FZD-targeted metabolic pathways were identified and compared to investigate the synergistic mechanism of QFD by computational systems analysis. Quantitative real-time PCR (qRT-PCR) was further employed to validate the key metabolic pathways at the level of the gene. Results The electrocardiogram combined with the biochemical analysis and histopathology showed that the protection effects were sorted as QFD > HQD ≈ FZD. A total of 41 metabolites contributing to DIC were identified in the mice serum, among which 32, 12 and 10 metabolites were significantly reverted by QFD, HQD and FZD, respectively. Metabolic pathway analysis revealed that DOX perturbed 12 metabolic pathways, and QFD, HQD, and FZD-treated groups could significantly reverse 12, 7 and 6 metabolic pathways of these 12 metabolic pathways. Metabolic pathway and qRT-PCR revealed that QFD could protect DIC mainly by regulating energy metabolism, amino acids metabolism, arachidonic acid metabolism and glycerophospholipid metabolism, and HQD and FZD mutually reinforced each other. Conclusion These evidences revealed that QFD was a promising drug candidate for DIC by maintaining metabolic homeostasis. Meanwhile, this work provided a useful approach for evaluating the efficacy and the synergistic effects of TCMs against cardiomyopathy .
研究领域:
药理学
·
中药学
·
代谢组学
·
心脏病学
·
系统生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
·
2020
·
IF 7.635
Cijun Shuai,Yun Cheng,Wenjing Yang,Pei Feng,Youwen Yang,Chongxian He,Fangwei Qi,Shuping Peng
Most of the existing scaffolds do not provide physical stimulation to cells in scaffold-guided bone regeneration. In this work, a superparamagnetic scaffold was fabricated using selective laser sintering with polyglycolic acid as matrix and Fe 3 O 4 nanoparticles as internal magnetic source. Additionally, a self-developed external static magnetic field (SMF) was used to provide external magnetic source. In this case, the magnetic moment of the Fe 3 O 4 nanoparticles rearranged along the direction of SMF, thereby generating a locally enhanced magnetic field. The enhanced magnetic field might directly stimulate cell surface receptors and activate downstream signaling pathways. As a consequence, the human umbilical cord-derived mesenchymal stem cells cultivated in the scaffold exhibited significantly enhanced gene expression of alkaline phosphatase , osteocalcin, type I collagen and runt-related transcription factor-2. In vivo tests revealed that the bone regeneration was accelerated with bone mineral density (with SMF: 515 ± 50 mg/cc, without SMF: 326 ± 15 mg/cc), percentage of bone volume/tissue volume (with SMF: 92 ± 3.5%, without SMF: 73 ± 5.2%) and new bone area fraction (with SMF: 86 ± 2.2%, without SMF: 70 ± 1.6%) after 8 weeks' implantation. The biodistribution and serum biochemistry analysis demonstrated that superparamagnetic scaffold possessed favorable biosafety.
研究领域:
生物材料
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组织工程
·
再生医学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2021
·
IF 4.36
Pu-Yang Gong,Yu-Jie Guo,Yu-Shan Tian,Li-Fei Gu,Jin Qi,Bo-Yang Yu
Ethnopharmacological relevance In traditional Chinese medicine (TCM) and modern pharmacodynamics , dried Rehmannia Radix (DRR) possesses prominent anti-thrombotic activity that decreases after processing by nine steaming and drying cycles to develop processed Rehmannia Radix (PRR). Due to the complexity of the DRR components, the chemical mechanism leading to efficacy changes of DRR caused by processing is still unclear. Aim of study This study aimed to trace the anti-thrombotic active compounds of DRR and different degrees of processed RR (PRR) and to evaluate the synergistic effects among different active components. Materials and methods The anti-thrombotic active chemical fraction of DRR extracts was evaluated. Targeted fractions of the processed products of RR were prepared at different processing stages. The changes in monosaccharides , oligosaccharides and secondary metabolites during processing were characterized by multidimensional high-performance liquid chromatography (HPLC). The anti-thrombotic effects of targeted fractions of different RR samples were evaluated by analyzing the length of tail thrombus (LT) and serum biochemical indicators in carrageenan-induced tail-thrombus mice. The spectrum-effect relationships were investigated by partial least squares regression (PLSR) analysis and gray correlation analysis (GRA). Finally, the active compounds were screened by spectrum-effect relationship analysis and validated in vivo , and their synergistic effects were determined by Webb's fraction multiplication method. Results Six ingredients highly associated with anti-thrombotic activities were screened out by the spectrum-effect relationship analysis, of which oligosaccharides (stachyose, sucrose and raffinose) and iridoid glycosides (catalpol, leonuride and melitoside) possessed a synergistic effect on tumor necrosis factors (TNF-α), interleukin 1β (IL-1β) and plasminogen activator inhibitor 1 (PAI-1)/tissue-type plasminogen activator (t-PA) ratio in vivo with synergistic coefficient (SC) > 1. Conclusion The main material basis of the anti-thrombotic activities of DRR is oligosaccharide components of stachyose, raffinose and sucrose, iridoid glycosides components of catalpol , leonuride and melittoside. The two kinds of components exert synergistic anti-thrombotic effects by inhibiting the expression of inflammatory factors and regulating the balance of the fibrinolysis system.
研究领域:
民族药理学
·
药理动力学
·
传统中药
·
化学分析
·
血栓研究
·
生物统计学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）